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Protein

Hepatoma-derived growth factor-related protein 2

Gene

Hdgfrp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with chromatin. Isoform 1 and isoform 3 bind to condensed chromatin in mitotic cells. Isoform 4 binds to non-condensed chromatin in the presence of HDGF.1 Publication

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatoma-derived growth factor-related protein 2
Short name:
HRP-2
Gene namesi
Name:Hdgfrp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1194492. Hdgfrp2.

Subcellular locationi

Nucleus 1 Publication
Note: Isoform 4 displays a punctate pattern and colocalizes with N-terminally processed HDFG.

GO - Cellular componenti

  1. mitochondrion Source: MGI
  2. nucleoplasm Source: MGI
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 669669Hepatoma-derived growth factor-related protein 2PRO_0000317644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei366 – 3661Phosphoserine5 Publications
Modified residuei367 – 3671Phosphoserine5 Publications
Modified residuei450 – 4501Phosphoserine1 Publication
Modified residuei628 – 6281PhosphoserineBy similarity
Modified residuei629 – 6291PhosphoserineBy similarity
Modified residuei635 – 6351Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UMU9.
PaxDbiQ3UMU9.
PRIDEiQ3UMU9.

PTM databases

PhosphoSiteiQ3UMU9.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiQ3UMU9.
CleanExiMM_HDGFRP2.
GenevestigatoriQ3UMU9.

Interactioni

Subunit structurei

Interacts with HDGF. Isoform 4 selectively interacts with HDGF (N-terminally processed form). Interacts with trimethylated 'Lys-36' of histone H3 (H3K36me3). Interacts with trimethylated 'Lys-79' of histone H3 (H3K79me3), but has higher affinity for H3K36me3 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
HdgfP518594EBI-7627961,EBI-2943087

Protein-protein interaction databases

IntActiQ3UMU9. 3 interactions.
MINTiMINT-4104792.

Structurei

3D structure databases

ProteinModelPortaliQ3UMU9.
SMRiQ3UMU9. Positions 1-93, 466-535.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 6458PWWPPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili550 – 57526Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi142 – 271130Ser-richAdd
BLAST
Compositional biasi322 – 36342Arg-richAdd
BLAST

Sequence similaritiesi

Belongs to the HDGF family.Curated
Contains 1 PWWP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG80217.
GeneTreeiENSGT00530000063013.
HOGENOMiHOG000230488.
HOVERGENiHBG099722.
InParanoidiQ3UMU9.
OMAiSDHSGLK.
OrthoDBiEOG7NKKMQ.
PhylomeDBiQ3UMU9.
TreeFamiTF105385.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR021567. LEDGF.
IPR000313. PWWP_dom.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF11467. LEDGF. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UMU9-1) [UniParc]FASTAAdd to basket

Also known as: Isoform a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPHAFKPGDL VFAKMKGYPH WPARIDDIAD GAVKPPPNKY PIFFFGTHET
60 70 80 90 100
AFLGPKDLFP YDKCKDKYGK PNKRKGFNEG LWEIQNNPHA SYSAPPPVSS
110 120 130 140 150
SDSEAPEADL GCGSDVDKDK ESRRVMTVTA VTTTATSDRM ESDSDSDKSS
160 170 180 190 200
DHSGLKRKTP VLKVSVSKRA RRASSDLDQA SVSPSEEDSE SPSESEKTSD
210 220 230 240 250
QDFTPEKKTA ARPPRRGPLG GRKKKKVPSA SDSDSKADSD GAKEEPVVTA
260 270 280 290 300
QPSPSSSSSS SSSSSSDSDV SVKKPPRGRK PAEKPPPKPR GRRPKPERPP
310 320 330 340 350
STSSSDSDSD SGEVDRISEW KRRDEERRRE LEARRRREQE EELRRLREQE
360 370 380 390 400
REEKERRKER AERGGSSGEE LEDEEPVKKR SRKARGRGTP SSSDSEPEGE
410 420 430 440 450
LGKEGKKLAK KSQLPGSESA RKPGQKEKRG RPDEKPRARP VKVERTRKRS
460 470 480 490 500
EGLSLERKGE KKKEPSVEER LQKLHSEIKF ALKVDNPDVR KCLSALEELG
510 520 530 540 550
TLQVTSQILQ KNTDVVATLK KIRRYKANKD VMAKAAEVYT RLKSRVLGPK
560 570 580 590 600
VEALQKVNKA GAEKERADNE KLEEQPGEQA PRELAEDEPS TDRSAPVNGE
610 620 630 640 650
ATSQKGENME DRAQEDGQDS EDGPRGGSSE ELHDSPRDNS DPAKPGNERQ
660
DHERTRLASE SANDDNEDS
Length:669
Mass (Da):74,291
Last modified:October 17, 2006 - v1
Checksum:i01228985D4616848
GO
Isoform 2 (identifier: Q3UMU9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     226-226: Missing.
     635-635: Missing.

Note: No experimental confirmation available.

Show »
Length:667
Mass (Da):74,075
Checksum:iE8B865571B20D78B
GO
Isoform 3 (identifier: Q3UMU9-3) [UniParc]FASTAAdd to basket

Also known as: Isoform b

The sequence of this isoform differs from the canonical sequence as follows:
     224-225: KK → KKHPTGYACPQ

Show »
Length:678
Mass (Da):75,246
Checksum:i034861DA18787C49
GO
Isoform 4 (identifier: Q3UMU9-4) [UniParc]FASTAAdd to basket

Also known as: Isoform c

The sequence of this isoform differs from the canonical sequence as follows:
     66-118: Missing.
     635-635: Missing.

Show »
Length:615
Mass (Da):68,530
Checksum:i71CD1E3AEE4761EE
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei66 – 11853Missing in isoform 4. 1 PublicationVSP_047648Add
BLAST
Alternative sequencei224 – 2252KK → KKHPTGYACPQ in isoform 3. 2 PublicationsVSP_031117
Alternative sequencei226 – 2261Missing in isoform 2. 1 PublicationVSP_031118
Alternative sequencei635 – 6351Missing in isoform 2 and isoform 4. 2 PublicationsVSP_031119

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63850 mRNA. Translation: BAA22896.1.
FN687734 mRNA. Translation: CBK52221.2.
AK143616 mRNA. Translation: BAE25467.1.
AK144669 mRNA. Translation: BAE25999.1.
AK146813 mRNA. Translation: BAE27453.1.
AK146918 mRNA. Translation: BAE27530.1.
BC003741 mRNA. Translation: AAH03741.1.
CCDSiCCDS28894.1. [Q3UMU9-1]
PIRiJC5662.
RefSeqiNP_032259.1. NM_008233.2. [Q3UMU9-1]
XP_006523769.1. XM_006523706.1. [Q3UMU9-3]
UniGeneiMm.279188.

Genome annotation databases

EnsembliENSMUST00000002911; ENSMUSP00000002911; ENSMUSG00000002833. [Q3UMU9-1]
GeneIDi15193.
KEGGimmu:15193.
UCSCiuc008dau.1. mouse. [Q3UMU9-1]
uc008dav.2. mouse. [Q3UMU9-2]
uc008daw.1. mouse. [Q3UMU9-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63850 mRNA. Translation: BAA22896.1.
FN687734 mRNA. Translation: CBK52221.2.
AK143616 mRNA. Translation: BAE25467.1.
AK144669 mRNA. Translation: BAE25999.1.
AK146813 mRNA. Translation: BAE27453.1.
AK146918 mRNA. Translation: BAE27530.1.
BC003741 mRNA. Translation: AAH03741.1.
CCDSiCCDS28894.1. [Q3UMU9-1]
PIRiJC5662.
RefSeqiNP_032259.1. NM_008233.2. [Q3UMU9-1]
XP_006523769.1. XM_006523706.1. [Q3UMU9-3]
UniGeneiMm.279188.

3D structure databases

ProteinModelPortaliQ3UMU9.
SMRiQ3UMU9. Positions 1-93, 466-535.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3UMU9. 3 interactions.
MINTiMINT-4104792.

PTM databases

PhosphoSiteiQ3UMU9.

Proteomic databases

MaxQBiQ3UMU9.
PaxDbiQ3UMU9.
PRIDEiQ3UMU9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002911; ENSMUSP00000002911; ENSMUSG00000002833. [Q3UMU9-1]
GeneIDi15193.
KEGGimmu:15193.
UCSCiuc008dau.1. mouse. [Q3UMU9-1]
uc008dav.2. mouse. [Q3UMU9-2]
uc008daw.1. mouse. [Q3UMU9-3]

Organism-specific databases

CTDi84717.
MGIiMGI:1194492. Hdgfrp2.

Phylogenomic databases

eggNOGiNOG80217.
GeneTreeiENSGT00530000063013.
HOGENOMiHOG000230488.
HOVERGENiHBG099722.
InParanoidiQ3UMU9.
OMAiSDHSGLK.
OrthoDBiEOG7NKKMQ.
PhylomeDBiQ3UMU9.
TreeFamiTF105385.

Miscellaneous databases

NextBioi287723.
PROiQ3UMU9.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UMU9.
CleanExiMM_HDGFRP2.
GenevestigatoriQ3UMU9.

Family and domain databases

Gene3Di1.20.930.10. 1 hit.
InterProiIPR021567. LEDGF.
IPR000313. PWWP_dom.
IPR017923. TFIIS_N.
[Graphical view]
PfamiPF11467. LEDGF. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEiPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hepatoma-derived growth factor belongs to a gene family in mice showing significant homology in the amino terminus."
    Izumoto Y., Kuroda T., Harada H., Kishimoto T., Nakamura H.
    Biochem. Biophys. Res. Commun. 238:26-32(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/c.
    Tissue: Testis.
  2. "Interaction of HRP-2 isoforms with HDGF: chromatin binding of a specific heteromer."
    Thakar K., Votteler I., Kelkar D., Shidore T., Gupta S., Kelm S., Dietz F.
    FEBS J. 279:737-751(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), ALTERNATIVE SPLICING, FUNCTION, INTERACTION WITH HDGF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: BALB/c.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J.
    Tissue: Heart, Lung and Spleen.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. "A differential phosphoproteomic analysis of retinoic acid-treated P19 cells."
    Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.
    J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Teratocarcinoma.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366; SER-367; SER-450 AND SER-635, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiHDGR2_MOUSE
AccessioniPrimary (citable) accession number: Q3UMU9
Secondary accession number(s): D6CHX5
, O35540, Q3UIH6, Q99L92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 17, 2006
Last modified: March 4, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.