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Q3UMU9 (HDGR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hepatoma-derived growth factor-related protein 2

Short name=HRP-2
Gene names
Name:Hdgfrp2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length669 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Associates with chromatin. Isoform 1 and isoform 3 bind to condensed chromatin in mitotic cells. Isoform 4 binds to non-condensed chromatin in the presence of HDGF. Ref.2

Subunit structure

Interacts with HDGF. Isoform 4 selectively interacts with HDGF (N-terminally processed form). Interacts with trimethylated 'Lys-36' of histone H3 (H3K36me3). Interacts with trimethylated 'Lys-79' of histone H3 (H3K79me3), but has higher affinity for H3K36me3 By similarity. Ref.2

Subcellular location

Nucleus. Note: Isoform 4 displays a punctate pattern and colocalizes with N-terminally processed HDFG. Ref.2

Tissue specificity

Ubiquitously expressed. Ref.2

Sequence similarities

Belongs to the HDGF family.

Contains 1 PWWP domain.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription, DNA-templated

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from direct assay Ref.2. Source: MGI

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

chromatin binding

Inferred from direct assay Ref.2. Source: MGI

protein binding

Inferred from physical interaction Ref.2. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HdgfP518594EBI-7627932,EBI-2943087

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3UMU9-1)

Also known as: Isoform a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3UMU9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     226-226: Missing.
     635-635: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q3UMU9-3)

Also known as: Isoform b;

The sequence of this isoform differs from the canonical sequence as follows:
     224-225: KK → KKHPTGYACPQ
Isoform 4 (identifier: Q3UMU9-4)

Also known as: Isoform c;

The sequence of this isoform differs from the canonical sequence as follows:
     66-118: Missing.
     635-635: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 669669Hepatoma-derived growth factor-related protein 2
PRO_0000317644

Regions

Domain7 – 6458PWWP
Coiled coil550 – 57526 Potential
Compositional bias142 – 271130Ser-rich
Compositional bias322 – 36342Arg-rich

Amino acid modifications

Modified residue3661Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Modified residue3671Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Modified residue4501Phosphoserine Ref.7
Modified residue6351Phosphoserine Ref.7

Natural variations

Alternative sequence66 – 11853Missing in isoform 4.
VSP_047648
Alternative sequence224 – 2252KK → KKHPTGYACPQ in isoform 3.
VSP_031117
Alternative sequence2261Missing in isoform 2.
VSP_031118
Alternative sequence6351Missing in isoform 2 and isoform 4.
VSP_031119

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Isoform a) [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 01228985D4616848

FASTA66974,291
        10         20         30         40         50         60 
MPHAFKPGDL VFAKMKGYPH WPARIDDIAD GAVKPPPNKY PIFFFGTHET AFLGPKDLFP 

        70         80         90        100        110        120 
YDKCKDKYGK PNKRKGFNEG LWEIQNNPHA SYSAPPPVSS SDSEAPEADL GCGSDVDKDK 

       130        140        150        160        170        180 
ESRRVMTVTA VTTTATSDRM ESDSDSDKSS DHSGLKRKTP VLKVSVSKRA RRASSDLDQA 

       190        200        210        220        230        240 
SVSPSEEDSE SPSESEKTSD QDFTPEKKTA ARPPRRGPLG GRKKKKVPSA SDSDSKADSD 

       250        260        270        280        290        300 
GAKEEPVVTA QPSPSSSSSS SSSSSSDSDV SVKKPPRGRK PAEKPPPKPR GRRPKPERPP 

       310        320        330        340        350        360 
STSSSDSDSD SGEVDRISEW KRRDEERRRE LEARRRREQE EELRRLREQE REEKERRKER 

       370        380        390        400        410        420 
AERGGSSGEE LEDEEPVKKR SRKARGRGTP SSSDSEPEGE LGKEGKKLAK KSQLPGSESA 

       430        440        450        460        470        480 
RKPGQKEKRG RPDEKPRARP VKVERTRKRS EGLSLERKGE KKKEPSVEER LQKLHSEIKF 

       490        500        510        520        530        540 
ALKVDNPDVR KCLSALEELG TLQVTSQILQ KNTDVVATLK KIRRYKANKD VMAKAAEVYT 

       550        560        570        580        590        600 
RLKSRVLGPK VEALQKVNKA GAEKERADNE KLEEQPGEQA PRELAEDEPS TDRSAPVNGE 

       610        620        630        640        650        660 
ATSQKGENME DRAQEDGQDS EDGPRGGSSE ELHDSPRDNS DPAKPGNERQ DHERTRLASE 


SANDDNEDS 

« Hide

Isoform 2 [UniParc].

Checksum: E8B865571B20D78B
Show »

FASTA66774,075
Isoform 3 (Isoform b) [UniParc].

Checksum: 034861DA18787C49
Show »

FASTA67875,246
Isoform 4 (Isoform c) [UniParc].

Checksum: 71CD1E3AEE4761EE
Show »

FASTA61568,530

References

« Hide 'large scale' references
[1]"Hepatoma-derived growth factor belongs to a gene family in mice showing significant homology in the amino terminus."
Izumoto Y., Kuroda T., Harada H., Kishimoto T., Nakamura H.
Biochem. Biophys. Res. Commun. 238:26-32(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
Tissue: Testis.
[2]"Interaction of HRP-2 isoforms with HDGF: chromatin binding of a specific heteromer."
Thakar K., Votteler I., Kelkar D., Shidore T., Gupta S., Kelm S., Dietz F.
FEBS J. 279:737-751(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4), ALTERNATIVE SPLICING, FUNCTION, INTERACTION WITH HDGF, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Strain: C57BL/6J.
Tissue: Heart, Lung and Spleen.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[6]"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells."
Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.
J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Teratocarcinoma.
[7]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366; SER-367; SER-450 AND SER-635, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[8]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366 AND SER-367, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D63850 mRNA. Translation: BAA22896.1.
FN687734 mRNA. Translation: CBK52221.2.
AK143616 mRNA. Translation: BAE25467.1.
AK144669 mRNA. Translation: BAE25999.1.
AK146813 mRNA. Translation: BAE27453.1.
AK146918 mRNA. Translation: BAE27530.1.
BC003741 mRNA. Translation: AAH03741.1.
CCDSCCDS28894.1. [Q3UMU9-1]
PIRJC5662.
RefSeqNP_032259.1. NM_008233.2. [Q3UMU9-1]
XP_006523769.1. XM_006523706.1. [Q3UMU9-3]
UniGeneMm.279188.

3D structure databases

ProteinModelPortalQ3UMU9.
SMRQ3UMU9. Positions 1-93, 466-535.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ3UMU9. 3 interactions.
MINTMINT-4104792.

PTM databases

PhosphoSiteQ3UMU9.

Proteomic databases

MaxQBQ3UMU9.
PaxDbQ3UMU9.
PRIDEQ3UMU9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002911; ENSMUSP00000002911; ENSMUSG00000002833. [Q3UMU9-1]
GeneID15193.
KEGGmmu:15193.
UCSCuc008dau.1. mouse. [Q3UMU9-1]
uc008dav.2. mouse. [Q3UMU9-2]
uc008daw.1. mouse. [Q3UMU9-3]

Organism-specific databases

CTD84717.
MGIMGI:1194492. Hdgfrp2.

Phylogenomic databases

eggNOGNOG80217.
GeneTreeENSGT00530000063013.
HOGENOMHOG000230488.
HOVERGENHBG099722.
InParanoidQ3UMU9.
OMASDHSGLK.
OrthoDBEOG7NKKMQ.
PhylomeDBQ3UMU9.
TreeFamTF105385.

Gene expression databases

BgeeQ3UMU9.
CleanExMM_HDGFRP2.
GenevestigatorQ3UMU9.

Family and domain databases

Gene3D1.20.930.10. 1 hit.
InterProIPR021567. LEDGF.
IPR000313. PWWP_dom.
IPR017923. TFIIS_N.
[Graphical view]
PfamPF11467. LEDGF. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTSM00293. PWWP. 1 hit.
[Graphical view]
PROSITEPS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287723.
PROQ3UMU9.
SOURCESearch...

Entry information

Entry nameHDGR2_MOUSE
AccessionPrimary (citable) accession number: Q3UMU9
Secondary accession number(s): D6CHX5 expand/collapse secondary AC list , O35540, Q3UIH6, Q99L92
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 17, 2006
Last modified: July 9, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot