Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ankyrin repeat domain-containing protein 27

Gene

Ankrd27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (By similarity). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (By similarity). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (PubMed:19403694, PubMed:21187289). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (PubMed:19745841, PubMed:22171327). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (By similarity). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (By similarity). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (PubMed:26620560).By similarity3 Publications

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB-KW
  • guanyl-nucleotide exchange factor activity Source: MGI
  • Rab GTPase binding Source: UniProtKB
  • SNARE binding Source: UniProtKB

GO - Biological processi

  • early endosome to late endosome transport Source: MGI
  • endosome to melanosome transport Source: UniProtKB
  • negative regulation of SNARE complex assembly Source: MGI
  • neuron projection morphogenesis Source: UniProtKB
  • positive regulation of dendrite morphogenesis Source: UniProtKB
  • positive regulation of GTPase activity Source: GO_Central
  • protein transport Source: UniProtKB-KW
  • retrograde transport, endosome to plasma membrane Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat domain-containing protein 27
Alternative name(s):
VPS9 domain-containing protein
VPS9-ankyrin-repeat protein
Gene namesi
Name:Ankrd27
Synonyms:Varp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2444103. Ankrd27.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • cytosol Source: GOC
  • early endosome Source: MGI
  • late endosome Source: MGI
  • lysosome Source: MGI
  • melanosome Source: UniProtKB
  • membrane Source: MGI
  • neuron projection Source: UniProtKB
  • plasma membrane Source: MGI
  • transport vesicle Source: UniProtKB
  • tubular endosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi310D → A: Inhibits dendrite formation. 1 Publication1
Mutagenesisi350Y → A: Inhibits dendrite formation. 1 Publication1
Mutagenesisi509Q → A: Disrupts interaction with RAB32 and RAB38; inhibits peripheral distribution of TYRP1 in melanocytes; no effect on dendrite formation in melanocytes. 2 Publications1
Mutagenesisi550Y → A: Disrupts interaction with RAB32 and RAB38; inhibits peripheral distribution of TYRP1 in melanocytes; no effect on dendrite formation in melanocytes. 2 Publications1
Mutagenesisi575W → A: Impairs interaction with RAB32 and RAB38. 1 Publication1
Mutagenesisi577Y → A: Impairs interaction with RAB32 and RAB38. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745571 – 1048Ankyrin repeat domain-containing protein 27Add BLAST1048

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei961PhosphoserineCombined sources1
Modified residuei969PhosphoserineBy similarity1
Modified residuei1022PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UMR0.
PaxDbiQ3UMR0.
PRIDEiQ3UMR0.

PTM databases

iPTMnetiQ3UMR0.
PhosphoSitePlusiQ3UMR0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034867.
CleanExiMM_ANKRD27.
ExpressionAtlasiQ3UMR0. baseline and differential.
GenevisibleiQ3UMR0. MM.

Interactioni

Subunit structurei

Interacts with RAB21 (GDP-bound form), VPS29, KIF5A, KIF5C, GOLGA4 (By similarity). Interacts with RAB32 (GTP-bound form), RAB38 (GTP-bound form), VAMP7 (PubMed:19403694, PubMed:21187289). Interacts with low affinity with RAB5 (By similarity). ANKRD27:RAB32 heterodimers can homodimerize to form tetramers (By similarity). Can interact with RAB38 or RAB32, VPS29 and VAMP7 simultaneously (By similarity). A decreased interaction with RAB32 seen in the presence of SGSM2 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rab38Q8QZZ85EBI-1993429,EBI-1993463

GO - Molecular functioni

  • Rab GTPase binding Source: UniProtKB
  • SNARE binding Source: UniProtKB

Protein-protein interaction databases

BioGridi232848. 1 interactor.
IntActiQ3UMR0. 2 interactors.
STRINGi10090.ENSMUSP00000041751.

Structurei

3D structure databases

ProteinModelPortaliQ3UMR0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini233 – 371VPS9PROSITE-ProRule annotationAdd BLAST139
Repeati396 – 426ANK 1Add BLAST31
Repeati462 – 491ANK 2Add BLAST30
Repeati495 – 524ANK 3Add BLAST30
Repeati528 – 557ANK 4Add BLAST30
Repeati564 – 593ANK 5Add BLAST30
Repeati597 – 627ANK 6Add BLAST31
Repeati668 – 698ANK 7Add BLAST31
Repeati742 – 771ANK 8Add BLAST30
Repeati775 – 804ANK 9Add BLAST30
Repeati808 – 837ANK 10Add BLAST30
Repeati841 – 870ANK 11Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 372Sufficient for GEF activity towards RAB21By similarityAdd BLAST372
Regioni396 – 460Sufficient for interaction with VPS29By similarityAdd BLAST65
Regioni451 – 729Interaction with RAB321 PublicationAdd BLAST279
Regioni451 – 600Interaction with RAB381 PublicationAdd BLAST150
Regioni658 – 707Required for interaction with VAMP7By similarity1 PublicationAdd BLAST50
Regioni692 – 745Sufficient for interaction with VPS29By similarityAdd BLAST54

Sequence similaritiesi

Contains 11 ANK repeats.PROSITE-ProRule annotation
Contains 1 VPS9 domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiENOG410IQ81. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00780000121977.
HOGENOMiHOG000033958.
HOVERGENiHBG080845.
InParanoidiQ3UMR0.
KOiK20175.
OMAiIDCLFKH.
OrthoDBiEOG091G07HY.
PhylomeDBiQ3UMR0.
TreeFamiTF351261.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR003123. VPS9.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 2 hits.
PF02204. VPS9. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
SM00167. VPS9. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 8 hits.
PS51205. VPS9. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UMR0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALYDEDLLK NPFYLALQKW RPDLCSKVAQ IHGIVLVPCR GSLPGSVQAS
60 70 80 90 100
CQFESYVLVP TEGHFQTLDG KAVVIEGNRI KLGAGFACLL SVPILFEETF
110 120 130 140 150
YNEKEESFSI LCIAHPLERR ETSEEPSAPA DPFSLKTIED VREFLGRHSE
160 170 180 190 200
KFDKNIASFH RTFRECERKS LRHHIDSVNA LYTKCLQQLL RDSHLKVLAK
210 220 230 240 250
QEAQMNLMKQ AVEMYVHHDI YDLIFKYVGT MEASEDAAFN KITRSLQDLQ
260 270 280 290 300
QKDIGVKPEF SFNIPRAKRE LGQLNKCTSP QQKLLCLRKV VQLMTQSPSQ
310 320 330 340 350
RVNLETMCAD DLLSVLLYLL VKTEIPNWMA NLSYIKNFRF SSSAKDELGY
360 370 380 390 400
CLTSVEAAIE YIRQGSLSTK TPDAEGFGDR LFLKQRMNLL SQMTSTPIDC
410 420 430 440 450
LFKHIASGNQ KEVERLLSQD DQDKDAMQKM CHPLCSCEDC EKLISGRLND
460 470 480 490 500
PSVVTPFSRD DRGQTPLHVA ALCGQASLID FLVSKGAVVN ATDYHGSTPL
510 520 530 540 550
HLACQKGFQS VTLLLLHYKA STEVQDNNGN TPLHLACTYG QEDCVKALVY
560 570 580 590 600
YDVQACRLDI GNEKGDTALH IAARWGYEGI IETLLQNGAP TAVQNRLKET
610 620 630 640 650
PLKCALNSKI LSIMEAHHLS SDRRPRPSEV PAQSPTRSVD SISQGSSTSS
660 670 680 690 700
FSSISVSFRQ EEVKKDYREV EKLLRAVADG DLEMVRYLLE WTEDDLDDVE
710 720 730 740 750
DAISTVDLEF CHPLCQCPKC APAQKLARIS ANGLSVNVTN QDGFSPLHMA
760 770 780 790 800
ALHGRTDLVP LLLKHGAYSG ARNTSQAVPL HLACQQGHFQ VAKCLLDSNA
810 820 830 840 850
KPNKKDLSGN TPLICACSAG HHEVAALLLQ HGASINACNN KGNTALHEAV
860 870 880 890 900
MGRHTLVVEL LLFYGASVDI LNKRQYTAAD CAEQDSKIME LLQVVPGCVA
910 920 930 940 950
SLDSVEEADH EGYVTVEIRR KWNPKMYNLP EEPLRRQFCL INPGGRFQER
960 970 980 990 1000
TSRETMGRDR SVPDLAGRSL QEPEKQRVTG KQSDLSDLSR YQTSEEGNKG
1010 1020 1030 1040
LPERPVSRQA APGHRPMVRR HTVNDAAILQ VPEVTVHLTT HEASVPQS
Length:1,048
Mass (Da):116,809
Last modified:February 6, 2007 - v2
Checksum:i63636820D0CC4D2C
GO
Isoform 2 (identifier: Q3UMR0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     670-724: Missing.

Note: No experimental confirmation available.
Show »
Length:993
Mass (Da):110,604
Checksum:i855BDE37439764D2
GO
Isoform 3 (identifier: Q3UMR0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     630-670: Missing.

Note: No experimental confirmation available.
Show »
Length:1,007
Mass (Da):112,333
Checksum:iD14EAB10424F63F5
GO

Sequence cautioni

The sequence AAP94281 differs from that shown. Reason: Frameshift at positions 920 and 929.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti281 – 310QQKLL…TMCAD → TAEAAVPEEGGPAHDTISQP ESELGDHVCR in BAC39537 (PubMed:16141072).CuratedAdd BLAST30
Sequence conflicti371 – 372TP → PQ in BAD21367 (PubMed:15449545).Curated2
Sequence conflicti373 – 377DAEGF → VRSPC in AAH16493 (PubMed:15489334).Curated5
Sequence conflicti601P → Q in BAE26038 (PubMed:16141072).Curated1
Sequence conflicti609K → KV in BAD21367 (PubMed:15449545).Curated1
Sequence conflicti730S → P in AAP94281 (PubMed:16525121).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022791630 – 670Missing in isoform 3. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_022792670 – 724Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336500 mRNA. Translation: AAP94281.1. Frameshift.
AK085796 mRNA. Translation: BAC39537.1.
AK144733 mRNA. Translation: BAE26038.1.
BC016493 mRNA. Translation: AAH16493.1.
BC065093 mRNA. Translation: AAH65093.1.
AK131117 Transcribed RNA. Translation: BAD21367.1.
CCDSiCCDS21154.1. [Q3UMR0-1]
RefSeqiNP_663608.3. NM_145633.3. [Q3UMR0-1]
NP_839994.1. NM_178263.3.
UniGeneiMm.272620.

Genome annotation databases

EnsembliENSMUST00000040844; ENSMUSP00000041751; ENSMUSG00000034867. [Q3UMR0-1]
ENSMUST00000190503; ENSMUSP00000140259; ENSMUSG00000034867. [Q3UMR0-2]
GeneIDi245886.
KEGGimmu:245886.
UCSCiuc009gkd.1. mouse. [Q3UMR0-1]
uc012fin.1. mouse. [Q3UMR0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY336500 mRNA. Translation: AAP94281.1. Frameshift.
AK085796 mRNA. Translation: BAC39537.1.
AK144733 mRNA. Translation: BAE26038.1.
BC016493 mRNA. Translation: AAH16493.1.
BC065093 mRNA. Translation: AAH65093.1.
AK131117 Transcribed RNA. Translation: BAD21367.1.
CCDSiCCDS21154.1. [Q3UMR0-1]
RefSeqiNP_663608.3. NM_145633.3. [Q3UMR0-1]
NP_839994.1. NM_178263.3.
UniGeneiMm.272620.

3D structure databases

ProteinModelPortaliQ3UMR0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi232848. 1 interactor.
IntActiQ3UMR0. 2 interactors.
STRINGi10090.ENSMUSP00000041751.

PTM databases

iPTMnetiQ3UMR0.
PhosphoSitePlusiQ3UMR0.

Proteomic databases

MaxQBiQ3UMR0.
PaxDbiQ3UMR0.
PRIDEiQ3UMR0.

Protocols and materials databases

DNASUi245886.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040844; ENSMUSP00000041751; ENSMUSG00000034867. [Q3UMR0-1]
ENSMUST00000190503; ENSMUSP00000140259; ENSMUSG00000034867. [Q3UMR0-2]
GeneIDi245886.
KEGGimmu:245886.
UCSCiuc009gkd.1. mouse. [Q3UMR0-1]
uc012fin.1. mouse. [Q3UMR0-2]

Organism-specific databases

CTDi84079.
MGIiMGI:2444103. Ankrd27.

Phylogenomic databases

eggNOGiENOG410IQ81. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00780000121977.
HOGENOMiHOG000033958.
HOVERGENiHBG080845.
InParanoidiQ3UMR0.
KOiK20175.
OMAiIDCLFKH.
OrthoDBiEOG091G07HY.
PhylomeDBiQ3UMR0.
TreeFamiTF351261.

Enzyme and pathway databases

ReactomeiR-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

ChiTaRSiAnkrd27. mouse.
PROiQ3UMR0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034867.
CleanExiMM_ANKRD27.
ExpressionAtlasiQ3UMR0. baseline and differential.
GenevisibleiQ3UMR0. MM.

Family and domain databases

Gene3Di1.25.40.20. 4 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR003123. VPS9.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 2 hits.
PF02204. VPS9. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 8 hits.
SM00167. VPS9. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 8 hits.
PS51205. VPS9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANR27_MOUSE
AccessioniPrimary (citable) accession number: Q3UMR0
Secondary accession number(s): Q6KAU0
, Q6P1F9, Q7TNY8, Q8BUD2, Q91W65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 30, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.