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Protein

WASH complex subunit 7

Gene

Kiaa1033

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875).By similarity1 Publication

GO - Biological processi

  • endosomal transport Source: UniProtKB
  • endosome organization Source: MGI
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
WASH complex subunit 7Curated
Gene namesi
Name:Kiaa1033Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2441787. A230046K03Rik.

Subcellular locationi

GO - Cellular componenti

  • early endosome Source: UniProtKB-SubCell
  • endosome Source: MGI
  • nucleoplasm Source: MGI
  • WASH complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 11731172WASH complex subunit 7PRO_0000282576Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei7 – 71PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3UMB9.
MaxQBiQ3UMB9.
PaxDbiQ3UMB9.
PRIDEiQ3UMB9.

PTM databases

iPTMnetiQ3UMB9.
PhosphoSiteiQ3UMB9.
SwissPalmiQ3UMB9.

Expressioni

Gene expression databases

BgeeiQ3UMB9.
CleanExiMM_A230046K03RIK.
GenevisibleiQ3UMB9. MM.

Interactioni

Subunit structurei

Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASH1, FAM21, KIAA1033/SWIP, KIAA0196/strumpellin and CCDC53. The WASH core complex associates via FAM21 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi235172. 1 interaction.
MINTiMINT-1858088.
STRINGi10090.ENSMUSP00000039322.

Structurei

3D structure databases

ProteinModelPortaliQ3UMB9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni705 – 1173469Sufficient for interaction with KIAA0196By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili27 – 5630Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the WASHS7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3578. Eukaryota.
ENOG410XRKB. LUCA.
GeneTreeiENSGT00390000002524.
HOGENOMiHOG000031074.
HOVERGENiHBG106578.
InParanoidiQ3UMB9.
KOiK18465.
OMAiQFLFDEH.
OrthoDBiEOG70087B.
PhylomeDBiQ3UMB9.
TreeFamiTF324604.

Family and domain databases

InterProiIPR028283. WASH-7_C.
IPR028282. WASH-7_central.
IPR028191. WASH-7_N.
IPR027307. WASH7.
[Graphical view]
PANTHERiPTHR31409:SF0. PTHR31409:SF0. 1 hit.
PfamiPF14746. WASH-7_C. 1 hit.
PF14744. WASH-7_mid. 1 hit.
PF14745. WASH-7_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3UMB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVDTLSPDW DFDRVDDGSQ KIHAEVQLKN YGRFLEEYTS QLRRIEDALD
60 70 80 90 100
DLIGDVWDFN LDPIALKLLP YEQSSLLELI KTENKVLNKV ITVYAALCCE
110 120 130 140 150
IKKLKYEAET KFYNGLLFYG EGATDSSMVE GDCQIQMGRF VSFLQELSCF
160 170 180 190 200
VTRCYEVVMN VIHQLAALYI SNKIGPKIIE TTGVHFQTMY EHLGELLTVL
210 220 230 240 250
LTLDEIVDNH VTLKDHWTMY KRLLKSVHHN PSKFGIQEEK LKPFEKFLLK
260 270 280 290 300
LEGQLLDGMI FQACIEQQFD SLNGGISVSK NSTFAEEFAH SIRSIFANVE
310 320 330 340 350
AKLGEPSEID QRDKYVGICG LFVLHFQIFR TVDKKFYKSL LDICKKVPAI
360 370 380 390 400
TLTANIIWFP DNFLIHKMPA AAKLLDRKSL QAIKIHRDTF LQQKAQSLNK
410 420 430 440 450
DVQSYYVFVS SWMMKMESML SKEQRMDTFA EDLTNRCNVF IQGFLYAYSI
460 470 480 490 500
STIIKTTMNL YMSMQKPMTK TSVKALCRLI ELLKAIEHMF YRRSMVVADS
510 520 530 540 550
VSHITQHLQH QALSSISVAK KRVISDKKYS EQRLDVLSAL VLAENTLNGP
560 570 580 590 600
STKQRRLIVS LALSVGTQMK TFKDEELFPL QVVMKKLDLI SELRERVQAQ
610 620 630 640 650
CDCCFLYWHR AVFPIYLDDV YENAVDAARL HYMFSALRDC VPAMMHSRHL
660 670 680 690 700
ESHELLLDCY DKEIMDILNE HLLDKLCKEI EKDLRLSVHT HLKLDDRNPF
710 720 730 740 750
KVGRKDLALF FSLNPIRFFN RFIDIRAYVT HYLDKTFYNL TTVALHDWAT
760 770 780 790 800
YSEMRNLATQ RYGLVMTEAH LPSQTLEQGL DVLEIMRNIH IFVSRYLYNL
810 820 830 840 850
NNQIFIERTS NNKHLNTINI RHIANSIRTH GTGIMNTTVN FTYQFLKKKF
860 870 880 890 900
YIFSQFMYDE HIKSRLIKDI RFFREIKDQN DHKYPFDRAE KFNRGIRKLG
910 920 930 940 950
ITPEGQSYLD QFRQLISQIG NAMGYIRMIR SGGLHCSSNA IRFVPDLEDI
960 970 980 990 1000
VSFEELVKEE GLAEETLRAA RHLDSVLSDH TRNSAEGTEY FKMLVDVFAP
1010 1020 1030 1040 1050
EFRRPKNIHL RNFYIIVPPL TLNFVEHSIS CKEKLNKKNK LGAAFTDDGF
1060 1070 1080 1090 1100
AMGVAYTLKL LDQYQEFDSL HWFQSVREKY IKEIRAVAKQ QNVQSTSQDE
1110 1120 1130 1140 1150
KLLQTMNLTQ KRLEVYLQEF ELLYFSLSSA RIFFRADKTA AEENQEKKEK
1160 1170
EEETKTSNGD GPESTVSADP VVK
Length:1,173
Mass (Da):136,370
Last modified:April 3, 2007 - v2
Checksum:iB4473C932FAE26AF
GO

Sequence cautioni

The sequence AAH20314.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH20314.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE36266.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC153508 Genomic DNA. No translation available.
AK038582 mRNA. Translation: BAC30055.1.
AK145000 mRNA. Translation: BAE26179.1.
AK161247 mRNA. Translation: BAE36266.1. Different initiation.
AK166233 mRNA. Translation: BAE38648.1.
BC020314 mRNA. Translation: AAH20314.1. Sequence problems.
AK129269 mRNA. Translation: BAC98079.1.
CCDSiCCDS36015.1.
RefSeqiNP_001028547.2. NM_001033375.2.
UniGeneiMm.491194.

Genome annotation databases

EnsembliENSMUST00000038388; ENSMUSP00000039322; ENSMUSG00000034560.
GeneIDi319277.
KEGGimmu:319277.
UCSCiuc007gkj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC153508 Genomic DNA. No translation available.
AK038582 mRNA. Translation: BAC30055.1.
AK145000 mRNA. Translation: BAE26179.1.
AK161247 mRNA. Translation: BAE36266.1. Different initiation.
AK166233 mRNA. Translation: BAE38648.1.
BC020314 mRNA. Translation: AAH20314.1. Sequence problems.
AK129269 mRNA. Translation: BAC98079.1.
CCDSiCCDS36015.1.
RefSeqiNP_001028547.2. NM_001033375.2.
UniGeneiMm.491194.

3D structure databases

ProteinModelPortaliQ3UMB9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi235172. 1 interaction.
MINTiMINT-1858088.
STRINGi10090.ENSMUSP00000039322.

PTM databases

iPTMnetiQ3UMB9.
PhosphoSiteiQ3UMB9.
SwissPalmiQ3UMB9.

Proteomic databases

EPDiQ3UMB9.
MaxQBiQ3UMB9.
PaxDbiQ3UMB9.
PRIDEiQ3UMB9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038388; ENSMUSP00000039322; ENSMUSG00000034560.
GeneIDi319277.
KEGGimmu:319277.
UCSCiuc007gkj.1. mouse.

Organism-specific databases

MGIiMGI:2441787. A230046K03Rik.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3578. Eukaryota.
ENOG410XRKB. LUCA.
GeneTreeiENSGT00390000002524.
HOGENOMiHOG000031074.
HOVERGENiHBG106578.
InParanoidiQ3UMB9.
KOiK18465.
OMAiQFLFDEH.
OrthoDBiEOG70087B.
PhylomeDBiQ3UMB9.
TreeFamiTF324604.

Miscellaneous databases

NextBioi394515.
PROiQ3UMB9.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UMB9.
CleanExiMM_A230046K03RIK.
GenevisibleiQ3UMB9. MM.

Family and domain databases

InterProiIPR028283. WASH-7_C.
IPR028282. WASH-7_central.
IPR028191. WASH-7_N.
IPR027307. WASH7.
[Graphical view]
PANTHERiPTHR31409:SF0. PTHR31409:SF0. 1 hit.
PfamiPF14746. WASH-7_C. 1 hit.
PF14744. WASH-7_mid. 1 hit.
PF14745. WASH-7_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1145 AND 818-1173.
    Strain: C57BL/6J.
    Tissue: Hypothalamus, Mammary gland and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 89-1145.
    Strain: Czech II.
    Tissue: Mammary tumor.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 124-1173.
    Tissue: Brain.
  5. "The Arp2/3 activator WASH controls the fission of endosomes through a large multiprotein complex."
    Derivery E., Sousa C., Gautier J.J., Lombard B., Loew D., Gautreau A.
    Dev. Cell 17:712-723(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE WASH COMPLEX.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiWASH7_MOUSE
AccessioniPrimary (citable) accession number: Q3UMB9
Secondary accession number(s): Q3TTQ9
, Q6ZPZ7, Q8CAK8, Q8CGK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: May 11, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.