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Protein

Large subunit GTPase 1 homolog

Gene

Lsg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase required for the XPO1/CRM1-mediated nuclear export of the 60S ribosomal subunit. Probably acts by mediating the release of NMD3 from the 60S ribosomal subunit after export into the cytoplasm (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi212 – 215GTPSequence analysis4
Nucleotide bindingi379 – 386GTPSequence analysis8
Nucleotide bindingi423 – 426GTPSequence analysis4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Large subunit GTPase 1 homolog (EC:3.6.1.-)
Gene namesi
Name:Lsg1
Synonyms:D16Bwg1547e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:107236. Lsg1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003245551 – 644Large subunit GTPase 1 homologAdd BLAST644

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei93PhosphoserineBy similarity1
Modified residuei97PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3UM18.
MaxQBiQ3UM18.
PaxDbiQ3UM18.
PeptideAtlasiQ3UM18.
PRIDEiQ3UM18.

PTM databases

iPTMnetiQ3UM18.
PhosphoSitePlusiQ3UM18.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022538.
CleanExiMM_LSG1.
ExpressionAtlasiQ3UM18. baseline and differential.
GenevisibleiQ3UM18. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112860.

Structurei

3D structure databases

ProteinModelPortaliQ3UM18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini164 – 430CP-type GPROSITE-ProRule annotationAdd BLAST267

Domaini

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Sequence similaritiesi

Belongs to the TRAFAC class YlqF/YawG GTPase family. LSG1 subfamily.PROSITE-ProRule annotation
Contains 1 CP-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1424. Eukaryota.
COG1161. LUCA.
GeneTreeiENSGT00530000063453.
HOGENOMiHOG000172552.
HOVERGENiHBG097657.
InParanoidiQ3UM18.
KOiK14539.
OMAiEDDWQTC.
OrthoDBiEOG091G07PS.
TreeFamiTF105747.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR030378. G_CP_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UM18-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRRRAPGGG SLGRVLIRQQ TQRSRSHRHT DSWLHTSELN DGYDWGRLNL
60 70 80 90 100
QSVTEQSSLE DFLATAELAG TEFVAEKLNI KFVPPEARTG LLSFEESQRI
110 120 130 140 150
KKLHEENRQF LCIPRRPNWD RKTSPEELKQ AEKDNFLKWR RQLVRLEEEQ
160 170 180 190 200
KLILTPFERN LDFWRQLWRV IERSDIVVQI VDARNPLLFR CEDLECYVKE
210 220 230 240 250
IDAAKENVIL INKADLLTAE QRFAWAVHFE KEGVKVIFWS ALAETDHLNG
260 270 280 290 300
DLKEEVDSVA GDTNKTESES SSLDANEIPH RDLISLSEES ASDSGDSKYE
310 320 330 340 350
DCQEDEEEDW QTCSEEDSVP EEEEGCNADS ETQNRKNAEN QQVNNDSYLV
360 370 380 390 400
SKQELLELFK KLHTGKKVKD GQLTVGLVGY PNVGKSSTIN TIMGNKKVSV
410 420 430 440 450
SATPGHTKHF QTLYVEPGLC LCDCPGLVMP SFVSTKAEMI CNGILPIDQM
460 470 480 490 500
RDHVPPVSLV CQNIPRRVLE VTYGINIIKP REDEDPYRPP TSEELLTAYG
510 520 530 540 550
CMRGFMTAHG QPDQPRSARY ILKDYVGGKL LYCHPPPGKD PVAFQHQHQQ
560 570 580 590 600
LLESKVKGGE LRLQPGKGRK AKQIENVVDK TFFHQENVRA LTKGVQAVMG
610 620 630 640
YKPGHGLVTA AAASAENVPG KPWKKHGNRN KKEKSRRLYK HLDV
Length:644
Mass (Da):73,157
Last modified:March 18, 2008 - v2
Checksum:iDB6962294D44EA96
GO
Isoform 2 (identifier: Q3UM18-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     146-174: Missing.

Note: No experimental confirmation available.
Show »
Length:615
Mass (Da):69,429
Checksum:iD6283E3F6D7B3493
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti284I → F in BAE26280 (PubMed:16141072).Curated1
Sequence conflicti291A → E in BAE26280 (PubMed:16141072).Curated1
Sequence conflicti298K → E in BAE26280 (PubMed:16141072).Curated1
Sequence conflicti320P → PE in BAE26280 (PubMed:16141072).Curated1
Sequence conflicti327N → P in BAE26280 (PubMed:16141072).Curated1
Sequence conflicti338A → T in BAE26280 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_032275146 – 174Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK135345 mRNA. Translation: BAE22495.1.
AK145186 mRNA. Translation: BAE26280.1.
AK146895 mRNA. Translation: BAE27512.1.
BC043724 mRNA. Translation: AAH43724.1.
BC080306 mRNA. Translation: AAH80306.1.
CCDSiCCDS37311.1. [Q3UM18-1]
RefSeqiNP_835170.1. NM_178069.5. [Q3UM18-1]
UniGeneiMm.567.

Genome annotation databases

EnsembliENSMUST00000117363; ENSMUSP00000112860; ENSMUSG00000022538. [Q3UM18-1]
GeneIDi224092.
KEGGimmu:224092.
UCSCiuc007ywv.1. mouse. [Q3UM18-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK135345 mRNA. Translation: BAE22495.1.
AK145186 mRNA. Translation: BAE26280.1.
AK146895 mRNA. Translation: BAE27512.1.
BC043724 mRNA. Translation: AAH43724.1.
BC080306 mRNA. Translation: AAH80306.1.
CCDSiCCDS37311.1. [Q3UM18-1]
RefSeqiNP_835170.1. NM_178069.5. [Q3UM18-1]
UniGeneiMm.567.

3D structure databases

ProteinModelPortaliQ3UM18.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112860.

PTM databases

iPTMnetiQ3UM18.
PhosphoSitePlusiQ3UM18.

Proteomic databases

EPDiQ3UM18.
MaxQBiQ3UM18.
PaxDbiQ3UM18.
PeptideAtlasiQ3UM18.
PRIDEiQ3UM18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117363; ENSMUSP00000112860; ENSMUSG00000022538. [Q3UM18-1]
GeneIDi224092.
KEGGimmu:224092.
UCSCiuc007ywv.1. mouse. [Q3UM18-1]

Organism-specific databases

CTDi55341.
MGIiMGI:107236. Lsg1.

Phylogenomic databases

eggNOGiKOG1424. Eukaryota.
COG1161. LUCA.
GeneTreeiENSGT00530000063453.
HOGENOMiHOG000172552.
HOVERGENiHBG097657.
InParanoidiQ3UM18.
KOiK14539.
OMAiEDDWQTC.
OrthoDBiEOG091G07PS.
TreeFamiTF105747.

Miscellaneous databases

ChiTaRSiLsg1. mouse.
PROiQ3UM18.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022538.
CleanExiMM_LSG1.
ExpressionAtlasiQ3UM18. baseline and differential.
GenevisibleiQ3UM18. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR030378. G_CP_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51721. G_CP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLSG1_MOUSE
AccessioniPrimary (citable) accession number: Q3UM18
Secondary accession number(s): Q68ED9, Q80V36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.