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Protein

Glycerol-3-phosphate dehydrogenase 1-like protein

Gene

Gpd1l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD(H) balance induced by DPD1L.By similarity

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431NADBy similarity
Binding sitei99 – 991NADBy similarity
Binding sitei122 – 1221SubstrateBy similarity
Binding sitei155 – 1551NAD; via amide nitrogenBy similarity
Active sitei206 – 2061Proton acceptorBy similarity
Binding sitei271 – 2711NADBy similarity
Binding sitei298 – 2981NAD; via amide nitrogenBy similarity
Binding sitei300 – 3001NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176NADBy similarity

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
  2. ion channel binding Source: MGI
  3. NAD binding Source: InterPro
  4. sodium channel regulator activity Source: MGI

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycerol-3-phosphate catabolic process Source: InterPro
  3. NADH metabolic process Source: MGI
  4. NAD metabolic process Source: MGI
  5. negative regulation of peptidyl-serine phosphorylation Source: MGI
  6. negative regulation of protein kinase C signaling Source: MGI
  7. positive regulation of protein localization to cell surface Source: MGI
  8. positive regulation of sodium ion transport Source: MGI
  9. regulation of heart rate Source: MGI
  10. regulation of sodium ion transmembrane transporter activity Source: MGI
  11. regulation of ventricular cardiac muscle cell membrane depolarization Source: MGI
  12. ventricular cardiac muscle cell action potential Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_188640. Synthesis of PA.
REACT_237947. Triglyceride Biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase 1-like protein (EC:1.1.1.8)
Gene namesi
Name:Gpd1l
Synonyms:Kiaa0089
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1289257. Gpd1l.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. glycerol-3-phosphate dehydrogenase complex Source: InterPro
  3. membrane Source: BHF-UCL
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Glycerol-3-phosphate dehydrogenase 1-like proteinPRO_0000286512Add
BLAST

Proteomic databases

MaxQBiQ3ULJ0.
PaxDbiQ3ULJ0.
PRIDEiQ3ULJ0.

PTM databases

PhosphoSiteiQ3ULJ0.

Expressioni

Gene expression databases

BgeeiQ3ULJ0.
CleanExiMM_GPD1L.
ExpressionAtlasiQ3ULJ0. baseline and differential.
GenevestigatoriQ3ULJ0.

Interactioni

Subunit structurei

Interacts with SCN5A.By similarity

Protein-protein interaction databases

IntActiQ3ULJ0. 1 interaction.
MINTiMINT-4113076.

Structurei

3D structure databases

ProteinModelPortaliQ3ULJ0.
SMRiQ3ULJ0. Positions 4-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni271 – 2722Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0240.
GeneTreeiENSGT00390000003114.
HOGENOMiHOG000246855.
HOVERGENiHBG003669.
InParanoidiQ3ULJ0.
KOiK00006.
OMAiKIFCKGQ.
OrthoDBiEOG7ZKSBS.
PhylomeDBiQ3ULJ0.
TreeFamiTF300836.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3ULJ0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAPLKVCI VGSGNWGSAV AKIIGSNVKT LQKFSSTVKM WVFEETVNGR
60 70 80 90 100
KLTDIINNDH ENVKYLPGHK LPENVVAVPN LSEAVQDADL LVFVIPHQFI
110 120 130 140 150
HKICDEITGR VPEKALGITL IKGIDEGPDG LKLISDIIRE KMGIDISVLM
160 170 180 190 200
GANIASEVAA EKFCETTIGS KVMQNGLLFK ELLQTPNFRI TVVDDADTVE
210 220 230 240 250
LCGALKNIVA VGAGFCDGLR CGDNTKAAVI RLGLMEMIAF AKIFCKGQVS
260 270 280 290 300
TATFLESCGV ADLITTCYGG RNRRVAEAFA RTGKTIEELE KELLNGQKLQ
310 320 330 340 350
GPQTSAEVYR ILRQKGLLDK FPLFTAVYQI CYEGRPVTQM LSCLQSHPEH

I
Length:351
Mass (Da):38,226
Last modified:May 15, 2007 - v2
Checksum:iBE192D41F592E577
GO
Isoform 2 (identifier: Q3ULJ0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     321-351: Missing.

Note: No experimental confirmation available.

Show »
Length:320
Mass (Da):34,634
Checksum:i690C96D09F52BC54
GO

Sequence cautioni

The sequence AAH37729.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAD32164.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261S → G in BAC36001. (PubMed:16141072)Curated
Sequence conflicti179 – 1791F → S in BAC36001. (PubMed:16141072)Curated
Sequence conflicti202 – 2021C → W in BAC36001. (PubMed:16141072)Curated
Sequence conflicti212 – 2121G → V in BAC36001. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei321 – 35131Missing in isoform 2. 1 PublicationVSP_025062Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172886 mRNA. Translation: BAD32164.1. Different initiation.
AK050572 mRNA. Translation: BAC34327.1.
AK075845 mRNA. Translation: BAC36001.1.
AK145475 mRNA. Translation: BAE26458.1.
BC037729 mRNA. Translation: AAH37729.1. Different initiation.
CCDSiCCDS23598.1. [Q3ULJ0-1]
RefSeqiNP_780589.3. NM_175380.5. [Q3ULJ0-1]
UniGeneiMm.38198.

Genome annotation databases

EnsembliENSMUST00000084853; ENSMUSP00000081913; ENSMUSG00000050627. [Q3ULJ0-2]
ENSMUST00000146623; ENSMUSP00000117509; ENSMUSG00000050627. [Q3ULJ0-1]
GeneIDi333433.
KEGGimmu:333433.
UCSCiuc009ryj.2. mouse. [Q3ULJ0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK172886 mRNA. Translation: BAD32164.1. Different initiation.
AK050572 mRNA. Translation: BAC34327.1.
AK075845 mRNA. Translation: BAC36001.1.
AK145475 mRNA. Translation: BAE26458.1.
BC037729 mRNA. Translation: AAH37729.1. Different initiation.
CCDSiCCDS23598.1. [Q3ULJ0-1]
RefSeqiNP_780589.3. NM_175380.5. [Q3ULJ0-1]
UniGeneiMm.38198.

3D structure databases

ProteinModelPortaliQ3ULJ0.
SMRiQ3ULJ0. Positions 4-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3ULJ0. 1 interaction.
MINTiMINT-4113076.

PTM databases

PhosphoSiteiQ3ULJ0.

Proteomic databases

MaxQBiQ3ULJ0.
PaxDbiQ3ULJ0.
PRIDEiQ3ULJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084853; ENSMUSP00000081913; ENSMUSG00000050627. [Q3ULJ0-2]
ENSMUST00000146623; ENSMUSP00000117509; ENSMUSG00000050627. [Q3ULJ0-1]
GeneIDi333433.
KEGGimmu:333433.
UCSCiuc009ryj.2. mouse. [Q3ULJ0-1]

Organism-specific databases

CTDi23171.
MGIiMGI:1289257. Gpd1l.
RougeiSearch...

Phylogenomic databases

eggNOGiCOG0240.
GeneTreeiENSGT00390000003114.
HOGENOMiHOG000246855.
HOVERGENiHBG003669.
InParanoidiQ3ULJ0.
KOiK00006.
OMAiKIFCKGQ.
OrthoDBiEOG7ZKSBS.
PhylomeDBiQ3ULJ0.
TreeFamiTF300836.

Enzyme and pathway databases

ReactomeiREACT_188640. Synthesis of PA.
REACT_237947. Triglyceride Biosynthesis.

Miscellaneous databases

NextBioi399982.
PROiQ3ULJ0.
SOURCEiSearch...

Gene expression databases

BgeeiQ3ULJ0.
CleanExiMM_GPD1L.
ExpressionAtlasiQ3ULJ0. baseline and differential.
GenevestigatoriQ3ULJ0.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR03376. glycerol3P_DH. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Fetal brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Stomach and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Eye.
  4. Lubec G., Klug S.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 207-220, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.

Entry informationi

Entry nameiGPD1L_MOUSE
AccessioniPrimary (citable) accession number: Q3ULJ0
Secondary accession number(s): Q6A0D2
, Q8BVZ7, Q8BWM5, Q8CFN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: February 4, 2015
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.