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Q3ULJ0 (GPD1L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase 1-like protein

EC=1.1.1.8
Gene names
Name:Gpd1l
Synonyms:Kiaa0089
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in regulating cardiac sodium current; decreased enzymatic activity with resulting increased levels of glycerol 3-phosphate activating the DPD1L-dependent SCN5A phosphorylation pathway, may ultimately lead to decreased sodium current; cardiac sodium current may also be reduced due to alterations of NAD(H) balance induced by DPD1L By similarity.

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Subunit structure

Interacts with SCN5A By similarity.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Sequence caution

The sequence AAH37729.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAD32164.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processNADH metabolic process

Inferred from mutant phenotype PubMed 19745168. Source: MGI

carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

glycerol-3-phosphate catabolic process

Inferred from electronic annotation. Source: InterPro

negative regulation of peptidyl-serine phosphorylation

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein kinase C signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein localization to cell surface

Inferred from electronic annotation. Source: Ensembl

positive regulation of sodium ion transport

Inferred from electronic annotation. Source: Ensembl

regulation of heart rate

Inferred from electronic annotation. Source: Ensembl

regulation of sodium ion transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

regulation of ventricular cardiac muscle cell membrane depolarization

Inferred from electronic annotation. Source: Ensembl

ventricular cardiac muscle cell action potential

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentglycerol-3-phosphate dehydrogenase complex

Inferred from electronic annotation. Source: InterPro

membrane

Inferred from direct assay PubMed 17967977. Source: BHF-UCL

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionNAD binding

Inferred from electronic annotation. Source: InterPro

glycerol-3-phosphate dehydrogenase [NAD+] activity

Inferred from electronic annotation. Source: UniProtKB-EC

sodium channel regulator activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3ULJ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3ULJ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     321-351: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351Glycerol-3-phosphate dehydrogenase 1-like protein
PRO_0000286512

Regions

Nucleotide binding12 – 176NAD By similarity
Region271 – 2722Substrate binding By similarity

Sites

Active site2061Proton acceptor By similarity
Binding site431NAD By similarity
Binding site991NAD By similarity
Binding site1221Substrate By similarity
Binding site1551NAD; via amide nitrogen By similarity
Binding site2711NAD By similarity
Binding site2981NAD; via amide nitrogen By similarity
Binding site3001NAD By similarity

Natural variations

Alternative sequence321 – 35131Missing in isoform 2.
VSP_025062

Experimental info

Sequence conflict261S → G in BAC36001. Ref.2
Sequence conflict1791F → S in BAC36001. Ref.2
Sequence conflict2021C → W in BAC36001. Ref.2
Sequence conflict2121G → V in BAC36001. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 15, 2007. Version 2.
Checksum: BE192D41F592E577

FASTA35138,226
        10         20         30         40         50         60 
MAAAPLKVCI VGSGNWGSAV AKIIGSNVKT LQKFSSTVKM WVFEETVNGR KLTDIINNDH 

        70         80         90        100        110        120 
ENVKYLPGHK LPENVVAVPN LSEAVQDADL LVFVIPHQFI HKICDEITGR VPEKALGITL 

       130        140        150        160        170        180 
IKGIDEGPDG LKLISDIIRE KMGIDISVLM GANIASEVAA EKFCETTIGS KVMQNGLLFK 

       190        200        210        220        230        240 
ELLQTPNFRI TVVDDADTVE LCGALKNIVA VGAGFCDGLR CGDNTKAAVI RLGLMEMIAF 

       250        260        270        280        290        300 
AKIFCKGQVS TATFLESCGV ADLITTCYGG RNRRVAEAFA RTGKTIEELE KELLNGQKLQ 

       310        320        330        340        350 
GPQTSAEVYR ILRQKGLLDK FPLFTAVYQI CYEGRPVTQM LSCLQSHPEH I 

« Hide

Isoform 2 [UniParc].

Checksum: 690C96D09F52BC54
Show »

FASTA32034,634

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Fetal brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Stomach and Thymus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Eye.
[4]Lubec G., Klug S.
Submitted (MAR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 207-220, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK172886 mRNA. Translation: BAD32164.1. Different initiation.
AK050572 mRNA. Translation: BAC34327.1.
AK075845 mRNA. Translation: BAC36001.1.
AK145475 mRNA. Translation: BAE26458.1.
BC037729 mRNA. Translation: AAH37729.1. Different initiation.
RefSeqNP_780589.3. NM_175380.5.
UniGeneMm.38198.

3D structure databases

ProteinModelPortalQ3ULJ0.
SMRQ3ULJ0. Positions 4-346.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ3ULJ0. 1 interaction.
MINTMINT-4113076.

PTM databases

PhosphoSiteQ3ULJ0.

Proteomic databases

PaxDbQ3ULJ0.
PRIDEQ3ULJ0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000084853; ENSMUSP00000081913; ENSMUSG00000050627. [Q3ULJ0-2]
ENSMUST00000146623; ENSMUSP00000117509; ENSMUSG00000050627. [Q3ULJ0-1]
GeneID333433.
KEGGmmu:333433.
UCSCuc009ryj.2. mouse. [Q3ULJ0-1]

Organism-specific databases

CTD23171.
MGIMGI:1289257. Gpd1l.
RougeSearch...

Phylogenomic databases

eggNOGCOG0240.
GeneTreeENSGT00390000003114.
HOGENOMHOG000246855.
HOVERGENHBG003669.
InParanoidQ3ULJ0.
KOK00006.
OMAKIFCKGQ.
OrthoDBEOG7ZKSBS.
PhylomeDBQ3ULJ0.
TreeFamTF300836.

Gene expression databases

ArrayExpressQ3ULJ0.
BgeeQ3ULJ0.
CleanExMM_GPD1L.
GenevestigatorQ3ULJ0.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR017751. G3P_DH_NAD-dep_euk.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR03376. glycerol3P_DH. 1 hit.
ProtoNetSearch...

Other

NextBio399982.
PROQ3ULJ0.
SOURCESearch...

Entry information

Entry nameGPD1L_MOUSE
AccessionPrimary (citable) accession number: Q3ULJ0
Secondary accession number(s): Q6A0D2 expand/collapse secondary AC list , Q8BVZ7, Q8BWM5, Q8CFN6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 15, 2007
Last modified: April 16, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot