Reviewed, UniProtKB/Swiss-Prot Q3ULJ0 (GPD1L_MOUSE)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase 1-like protein
    EC=1.1.1.8

Gene names

Name:	Gpd1l
Synonyms:	Kiaa0089

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH.
Subcellular location	CytoplasmPotential.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3ULJ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3ULJ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     321-351: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Glycerol-3-phosphate dehydrogenase 1-like protein		PRO_0000286512
Regions
	Nucleotide binding	12 – 17	6	NAD By similarity
	Region	271 – 272	2	Substrate binding By similarity
Sites
	Active site	206	1	Proton acceptor By similarity
	Binding site	43	1	NAD By similarity
	Binding site	99	1	NAD By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	155	1	NAD; via amide nitrogen By similarity
	Binding site	271	1	NAD By similarity
	Binding site	298	1	NAD; via amide nitrogen By similarity
	Binding site	300	1	NAD By similarity
Natural variations
	Alternative sequence	321 – 351	31	Missing in isoform 2.		VSP_025062
Experimental info
	Sequence conflict	26	1	S → G in BAC36001. Ref.2
	Sequence conflict	179	1	F → S in BAC36001. Ref.2
	Sequence conflict	202	1	C → W in BAC36001. Ref.2
	Sequence conflict	212	1	G → V in BAC36001. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: BE192D41F592E577	FASTA	351	38,226
	10 20 30 40 50 60 MAAAPLKVCI VGSGNWGSAV AKIIGSNVKT LQKFSSTVKM WVFEETVNGR KLTDIINNDH 70 80 90 100 110 120 ENVKYLPGHK LPENVVAVPN LSEAVQDADL LVFVIPHQFI HKICDEITGR VPEKALGITL 130 140 150 160 170 180 IKGIDEGPDG LKLISDIIRE KMGIDISVLM GANIASEVAA EKFCETTIGS KVMQNGLLFK 190 200 210 220 230 240 ELLQTPNFRI TVVDDADTVE LCGALKNIVA VGAGFCDGLR CGDNTKAAVI RLGLMEMIAF 250 260 270 280 290 300 AKIFCKGQVS TATFLESCGV ADLITTCYGG RNRRVAEAFA RTGKTIEELE KELLNGQKLQ 310 320 330 340 350 GPQTSAEVYR ILRQKGLLDK FPLFTAVYQI CYEGRPVTQM LSCLQSHPEH I
	Isoform 2 [UniParc]. Checksum: 690C96D09F52BC54		320	34,634
	10 20 30 40 50 60 MAAAPLKVCI VGSGNWGSAV AKIIGSNVKT LQKFSSTVKM WVFEETVNGR KLTDIINNDH 70 80 90 100 110 120 ENVKYLPGHK LPENVVAVPN LSEAVQDADL LVFVIPHQFI HKICDEITGR VPEKALGITL 130 140 150 160 170 180 IKGIDEGPDG LKLISDIIRE KMGIDISVLM GANIASEVAA EKFCETTIGS KVMQNGLLFK 190 200 210 220 230 240 ELLQTPNFRI TVVDDADTVE LCGALKNIVA VGAGFCDGLR CGDNTKAAVI RLGLMEMIAF 250 260 270 280 290 300 AKIFCKGQVS TATFLESCGV ADLITTCYGG RNRRVAEAFA RTGKTIEELE KELLNGQKLQ 310 320 GPQTSAEVYR ILRQKGLLDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Fetal brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Stomach and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Eye.
[4]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 207-220, MASS SPECTROMETRY. Tissue: Hippocampus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK172886 mRNA. Translation: BAD32164.1. Different initiation. AK050572 mRNA. Translation: BAC34327.1. AK075845 mRNA. Translation: BAC36001.1. AK145475 mRNA. Translation: BAE26458.1. BC037729 mRNA. Translation: AAH37729.1. Different initiation.
RefSeq	NP_780589.2.
UniGene	Mm.38198
3D structure databases
SMR	Q3ULJ0. Positions 4-346.
ModBase	Search...
PTM databases
PhosphoSite	Q3ULJ0.
Genome annotation databases
Ensembl	ENSMUSG00000050627. Mus musculus. [Contig view]
GeneID	333433.
KEGG	mmu:333433.
NMPDR	fig|10090.3.peg.20992.
Organism-specific databases
MGI	MGI:1289257. Gpd1l.
Rouge	Search...
Phylogenomic databases
HOVERGEN	Q3ULJ0.
Gene expression databases
ArrayExpress	Q3ULJ0.
CleanEx	MM_GPD1L.
Family and domain databases
InterPro	IPR017751. NAD-dep_Gly3P_DH_euk. IPR006168. NAD-dep_Gly3P_DHase. IPR011128. NAD-dep_Gly3P_DHase_N. IPR006109. NAD_Gly3P_DHase_C. [Graphical view]
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00957. NAD_G3PDH. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	399982.
SOURCE	Search...

Entry information

Entry name

GPD1L_MOUSE

Accession

Primary (citable) accession number: Q3ULJ0
Secondary accession number(s): Q6A0D2 , Q8BVZ7, Q8BWM5, Q8CFN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	November 25, 2008
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)