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Q3ULB5 (PAK6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase PAK 6

EC=2.7.11.1
Alternative name(s):
p21-activated kinase 6
Short name=PAK-6
Gene names
Name:Pak6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length682 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Interacts with the androgen receptor AR. Interacts with IQGAP1 and PPM1B By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Note: Cotranslocates into nucleus with AR in response to androgen induction By similarity.

Post-translational modification

Autophosphorylated. Phosphorylated by MAP2K6/MAPKK6, leading to PAK6 activation By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 CRIB domain.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 682682Serine/threonine-protein kinase PAK 6
PRO_0000278117

Regions

Domain12 – 2514CRIB
Domain408 – 659252Protein kinase
Nucleotide binding414 – 4229ATP By similarity
Region26 – 407382Linker By similarity
Compositional bias4 – 85Poly-Lys

Sites

Active site5271Proton acceptor By similarity
Binding site4371ATP By similarity

Amino acid modifications

Modified residue5611Phosphoserine; by autocatalysis By similarity

Experimental info

Sequence conflict6671C → R in BAE34737. Ref.1
Sequence conflict6671C → R in BAE34725. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q3ULB5 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: F9D415D49A130C0C

FASTA68274,867
        10         20         30         40         50         60 
MFRKKKKKRP EISAPQNFQH RVHTSFDPKE GKFVGLPPQW QNILDTLRRP KPVVDPSRIT 

        70         80         90        100        110        120 
RVQLQPMKTV VRGSSVPTEG YISGLLNDIQ KLSVISSNTL RGRSPTSRRR AQSLGLLGDD 

       130        140        150        160        170        180 
QWAADPDMYL QSPQSEHTDP HGLYLSCNGG TPAGHRQVPW PEPQSPQALP NGMAAKAQSL 

       190        200        210        220        230        240 
GPAEFQGASQ RCLQQLGACL QSSPPGTSPP MATGRRGVKV AKHSSEEARP QSCLVGSAIG 

       250        260        270        280        290        300 
RPGGEGSPSP KNQESSLKHR LFRSMFLSTP ATGAASSSKP VPLPQNKPNS AFRPPQKDSS 

       310        320        330        340        350        360 
SNLVAKAQSL PSEQPMGTFS PLTTSDTSSP QKSLRTAPAA GPLPGRSSPA GSPRTRHAQI 

       370        380        390        400        410        420 
STSNLYLPQD PTVAKGALGG EDTGIVTHEQ FKAALRMVVD QGDPRLLLDS YVKIGEGSTG 

       430        440        450        460        470        480 
IVCLAREKHS GRQVAVKMMD LRKQQRRELL FNEVVIMRDY QHLNVVEMYK SYLVGEELWV 

       490        500        510        520        530        540 
LMEFLQGGAL TDIISQVRLN EEQIATVCEA VLQALAYLHA QGVIHRDIKS DSILLTLDGR 

       550        560        570        580        590        600 
VKLSDFGFCA QISKDVPKRK SLVGTPYWMA PEVISRSLYA TEVDIWSLGI MVIEMVDGEP 

       610        620        630        640        650        660 
PYFSDSPVQA MKRLRDSAPP KLKNSYKVSP VLRDFLDRML VREPQERATA QELLDHPFLL 

       670        680 
QTGLPECLVP LIQLYRKQTS TC 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Visual cortex.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK145601 mRNA. Translation: BAE26534.1.
AK158913 mRNA. Translation: BAE34725.1.
AK158944 mRNA. Translation: BAE34737.1.
AL845470 Genomic DNA. Translation: CAM17416.1.
CCDSCCDS16580.1.
RefSeqNP_001028426.2. NM_001033254.3.
NP_001139326.1. NM_001145854.1.
UniGeneMm.241857.

3D structure databases

ProteinModelPortalQ3ULB5.
SMRQ3ULB5. Positions 11-45, 386-675.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ3ULB5.

Proteomic databases

PRIDEQ3ULB5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000099557; ENSMUSP00000097153; ENSMUSG00000074923.
ENSMUST00000110853; ENSMUSP00000106477; ENSMUSG00000074923.
GeneID214230.
KEGGmmu:214230.
UCSCuc008lsf.2. mouse.

Organism-specific databases

CTD56924.
MGIMGI:2679420. Pak6.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00670000097939.
HOGENOMHOG000234205.
HOVERGENHBG108518.
InParanoidQ3ULB5.
KOK05735.
OMAVPWPEPQ.
OrthoDBEOG73804D.
PhylomeDBQ3ULB5.
TreeFamTF105352.

Gene expression databases

BgeeQ3ULB5.
CleanExMM_PAK6.
GenevestigatorQ3ULB5.

Family and domain databases

Gene3D3.90.810.10. 1 hit.
InterProIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00786. PBD. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00285. PBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio374228.
PROQ3ULB5.
SOURCESearch...

Entry information

Entry namePAK6_MOUSE
AccessionPrimary (citable) accession number: Q3ULB5
Secondary accession number(s): Q3TY26
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot