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Protein

NEDD8-activating enzyme E1 regulatory subunit

Gene

Nae1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M.UniRule annotation

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathwayUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00885.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-activating enzyme E1 regulatory subunitUniRule annotation
Gene namesi
Name:Nae1Imported
Synonyms:Appbp1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2384561. Nae1.

PTM / Processingi

Proteomic databases

PRIDEiQ3UK27.

Expressioni

Gene expression databases

BgeeiQ3UK27.
GenevisibleiQ3UK27. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034349.

Structurei

3D structure databases

SMRiQ3UK27. Positions 9-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 518506ThiFInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2016. Eukaryota.
COG0476. LUCA.
HOVERGENiHBG079761.
KOiK04532.
OMAiCRKANIV.
PhylomeDBiQ3UK27.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR030667. APP-BP1.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
PIRSFiPIRSF039099. APP-BP1. 1 hit.
SUPFAMiSSF69572. SSF69572. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3UK27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQPGKILKE QKYDRQLRLW GDHGQEALES AHVCLINATA TGTEILKNLV
60 70 80 90 100
LPGIGSFTII DGNLVSGEDA GNNFFLQKSS IGKNRAQAAM EFLQELNSDV
110 120 130 140 150
SGSFVEESPE NLLDNDPSFF CRFTIVVATQ LLESTLLRLA DVLWNSQIPL
160 170 180 190 200
LICRTYGLVG YMRIIIKEHP VIESHPDNAL EDLRLDKPFP ELREHLQSYD
210 220 230 240 250
LDHMEKKDHS HTPWIVIIAK YLAQWYNETN GRIPKSYKEK EDFRDLIRQG
260 270 280 290 300
ILKNENGAPE DEENFEEAIK NVNTALNTTQ IPSSIEDIFN DDRCINITKQ
310 320 330 340 350
TPTFWILARA LKEFVAKEGQ GNLPVRGTIP DMIADSNKYI KLQNVYREKA
360 370 380 390 400
KKDAAAVGNH VAKLLQSVGQ APESISEKEL KLLCSNSAFL RVVRCRSLAE
410 420 430 440 450
EYGLDTVNKD EIISSMDNPD NEIVLYLMLR AVDRFHKQHG RYPGVSNYQV
460 470 480 490 500
EEDIGKLKSC LTGFLQEYGL SVMVKDDYVH EFCRYGAAEP HTIAAFLGGA
510 520 530
AAQEVIKIIT KQFVIFNNTY IYSGMSQTSA TFQL
Length:534
Mass (Da):60,274
Last modified:October 11, 2005 - v1
Checksum:i5CFCABFE5FA7AC62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC130543 Genomic DNA. No translation available.
AK146202 mRNA. Translation: BAE26975.1.
RefSeqiNP_659180.1. NM_144931.3.
UniGeneiMm.237670.

Genome annotation databases

EnsembliENSMUST00000034349; ENSMUSP00000034349; ENSMUSG00000031878.
GeneIDi234664.
KEGGimmu:234664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC130543 Genomic DNA. No translation available.
AK146202 mRNA. Translation: BAE26975.1.
RefSeqiNP_659180.1. NM_144931.3.
UniGeneiMm.237670.

3D structure databases

SMRiQ3UK27. Positions 9-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034349.

Proteomic databases

PRIDEiQ3UK27.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034349; ENSMUSP00000034349; ENSMUSG00000031878.
GeneIDi234664.
KEGGimmu:234664.

Organism-specific databases

CTDi8883.
MGIiMGI:2384561. Nae1.

Phylogenomic databases

eggNOGiKOG2016. Eukaryota.
COG0476. LUCA.
HOVERGENiHBG079761.
KOiK04532.
OMAiCRKANIV.
PhylomeDBiQ3UK27.

Enzyme and pathway databases

UniPathwayiUPA00885.

Miscellaneous databases

NextBioi382253.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UK27.
GenevisibleiQ3UK27. MM.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR030667. APP-BP1.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
PIRSFiPIRSF039099. APP-BP1. 1 hit.
SUPFAMiSSF69572. SSF69572. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "High-efficiency full-length cDNA cloning."
    Carninci P., Hayashizaki Y.
    Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DBA/2Imported.
  2. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
    Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
    Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DBA/2Imported.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DBA/2Imported.
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DBA/2Imported.
  5. "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
    The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
    Nature 420:563-573(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DBA/2Imported.
  6. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DBA/2Imported.
  7. "The Transcriptional Landscape of the Mammalian Genome."
    The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
    Science 309:1559-1563(2005)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DBA/2Imported.
  8. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DBA/2Imported.
  9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6JImported.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Ensembl
    Submitted (AUG-2015) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: C57BL/6JImported.

Entry informationi

Entry nameiQ3UK27_MOUSE
AccessioniPrimary (citable) accession number: Q3UK27
Entry historyi
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: April 13, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.