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Protein

NEDD8-activating enzyme E1 regulatory subunit

Gene

Nae1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M.UniRule annotation

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathwayUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00885

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-activating enzyme E1 regulatory subunitUniRule annotation
Gene namesi
Name:Nae1Imported
Synonyms:Appbp1Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:2384561 Nae1

PTM / Processingi

Proteomic databases

PRIDEiQ3UK27

Expressioni

Gene expression databases

BgeeiENSMUSG00000031878
ExpressionAtlasiQ3UK27 baseline and differential
GenevisibleiQ3UK27 MM

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ3UK27
SMRiQ3UK27
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 518ThiFInterPro annotationAdd BLAST506

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2016 Eukaryota
COG0476 LUCA
HOVERGENiHBG079761
KOiK04532
OMAiNDDRCIN
OrthoDBiEOG091G05WI
PhylomeDBiQ3UK27

Family and domain databases

InterProiView protein in InterPro
IPR030667 APP-BP1
IPR000594 ThiF_NAD_FAD-bd
IPR035985 Ubiquitin-activating_enz
PANTHERiPTHR10953:SF29 PTHR10953:SF29, 1 hit
PfamiView protein in Pfam
PF00899 ThiF, 1 hit
PIRSFiPIRSF039099 APP-BP1, 1 hit
SUPFAMiSSF69572 SSF69572, 1 hit

Sequencei

Sequence statusi: Complete.

Q3UK27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQPGKILKE QKYDRQLRLW GDHGQEALES AHVCLINATA TGTEILKNLV
60 70 80 90 100
LPGIGSFTII DGNLVSGEDA GNNFFLQKSS IGKNRAQAAM EFLQELNSDV
110 120 130 140 150
SGSFVEESPE NLLDNDPSFF CRFTIVVATQ LLESTLLRLA DVLWNSQIPL
160 170 180 190 200
LICRTYGLVG YMRIIIKEHP VIESHPDNAL EDLRLDKPFP ELREHLQSYD
210 220 230 240 250
LDHMEKKDHS HTPWIVIIAK YLAQWYNETN GRIPKSYKEK EDFRDLIRQG
260 270 280 290 300
ILKNENGAPE DEENFEEAIK NVNTALNTTQ IPSSIEDIFN DDRCINITKQ
310 320 330 340 350
TPTFWILARA LKEFVAKEGQ GNLPVRGTIP DMIADSNKYI KLQNVYREKA
360 370 380 390 400
KKDAAAVGNH VAKLLQSVGQ APESISEKEL KLLCSNSAFL RVVRCRSLAE
410 420 430 440 450
EYGLDTVNKD EIISSMDNPD NEIVLYLMLR AVDRFHKQHG RYPGVSNYQV
460 470 480 490 500
EEDIGKLKSC LTGFLQEYGL SVMVKDDYVH EFCRYGAAEP HTIAAFLGGA
510 520 530
AAQEVIKIIT KQFVIFNNTY IYSGMSQTSA TFQL
Length:534
Mass (Da):60,274
Last modified:October 11, 2005 - v1
Checksum:i5CFCABFE5FA7AC62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146202 mRNA Translation: BAE26975.1
RefSeqiNP_659180.1, NM_144931.3
UniGeneiMm.237670

Genome annotation databases

GeneIDi234664
KEGGimmu:234664

Similar proteinsi

Entry informationi

Entry nameiQ3UK27_MOUSE
AccessioniPrimary (citable) accession number: Q3UK27
Entry historyiIntegrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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