ID   Q3UK15_MOUSE            Unreviewed;       213 AA.
AC   Q3UK15;
DT   11-OCT-2005, integrated into UniProtKB/TrEMBL.
DT   11-OCT-2005, sequence version 1.
DT   24-JAN-2024, entry version 105.
DE   RecName: Full=TLC domain-containing protein {ECO:0000259|PROSITE:PS50922};
GN   Name=Cers5 {ECO:0000313|MGI:MGI:1919199};
GN   Synonyms=Lass5 {ECO:0000313|MGI:MGI:1919199};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|EMBL:BAE26988.1};
RN   [1] {ECO:0000313|EMBL:BAE26988.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DBA/2 {ECO:0000313|EMBL:BAE26988.1};
RX   PubMed=10349636; DOI=10.1016/S0076-6879(99)03004-9;
RA   Carninci P., Hayashizaki Y.;
RT   "High-efficiency full-length cDNA cloning.";
RL   Methods Enzymol. 303:19-44(1999).
RN   [2] {ECO:0000313|EMBL:BAE26988.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DBA/2 {ECO:0000313|EMBL:BAE26988.1};
RX   PubMed=11042159; DOI=10.1101/gr.145100;
RA   Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M.,
RA   Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.;
RT   "Normalization and subtraction of cap-trapper-selected cDNAs to prepare
RT   full-length cDNA libraries for rapid discovery of new genes.";
RL   Genome Res. 10:1617-1630(2000).
RN   [3] {ECO:0000313|EMBL:BAE26988.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DBA/2 {ECO:0000313|EMBL:BAE26988.1};
RX   PubMed=11076861; DOI=10.1101/gr.152600;
RA   Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P.,
RA   Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N.,
RA   Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A., Yamamoto R.,
RA   Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S.,
RA   Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y.,
RA   Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y.,
RA   Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.;
RT   "RIKEN integrated sequence analysis (RISA) system--384-format sequencing
RT   pipeline with 384 multicapillary sequencer.";
RL   Genome Res. 10:1757-1771(2000).
RN   [4] {ECO:0000313|EMBL:BAE26988.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DBA/2 {ECO:0000313|EMBL:BAE26988.1};
RX   PubMed=11217851; DOI=10.1038/35055500;
RG   The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium;
RT   "Functional annotation of a full-length mouse cDNA collection.";
RL   Nature 409:685-690(2001).
RN   [5] {ECO:0000313|EMBL:BAE26988.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DBA/2 {ECO:0000313|EMBL:BAE26988.1};
RX   PubMed=12466851; DOI=10.1038/nature01266;
RG   The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team;
RT   "Analysis of the mouse transcriptome based on functional annotation of
RT   60,770 full-length cDNAs.";
RL   Nature 420:563-573(2002).
RN   [6] {ECO:0000313|EMBL:BAE26988.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DBA/2 {ECO:0000313|EMBL:BAE26988.1};
RA   Arakawa T., Carninci P., Fukuda S., Hashizume W., Hayashida K., Hori F.,
RA   Iida J., Imamura K., Imotani K., Itoh M., Kanagawa S., Kawai J., Kojima M.,
RA   Konno H., Murata M., Nakamura M., Ninomiya N., Nishiyori H., Nomura K.,
RA   Ohno M., Sakazume N., Sano H., Sasaki D., Shibata K., Shiraki T.,
RA   Tagami M., Tagami Y., Waki K., Watahiki A., Muramatsu M., Hayashizaki Y.;
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:BAE26988.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DBA/2 {ECO:0000313|EMBL:BAE26988.1};
RG   The FANTOM Consortium;
RG   Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group);
RT   "The Transcriptional Landscape of the Mammalian Genome.";
RL   Science 309:1559-1563(2005).
RN   [8] {ECO:0000313|EMBL:BAE26988.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=DBA/2 {ECO:0000313|EMBL:BAE26988.1};
RX   PubMed=16141073; DOI=10.1126/science.1112009;
RG   RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium;
RT   "Antisense Transcription in the Mammalian Transcriptome.";
RL   Science 309:1564-1566(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=octadecanoyl-CoA + sphinganine = CoA + H(+) + N-
CC         (octadecanoyl)-sphinganine; Xref=Rhea:RHEA:36547, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57394, ChEBI:CHEBI:57817,
CC         ChEBI:CHEBI:67033; Evidence={ECO:0000256|ARBA:ARBA00024546};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36548;
CC         Evidence={ECO:0000256|ARBA:ARBA00024546};
CC   -!- PATHWAY: Lipid metabolism; sphingolipid metabolism.
CC       {ECO:0000256|ARBA:ARBA00004760}.
CC   -!- PATHWAY: Sphingolipid metabolism. {ECO:0000256|ARBA:ARBA00004991}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK146220; BAE26988.1; -; mRNA.
DR   AlphaFoldDB; Q3UK15; -.
DR   PeptideAtlas; Q3UK15; -.
DR   AGR; MGI:1919199; -.
DR   MGI; MGI:1919199; Cers5.
DR   UniPathway; UPA00222; -.
DR   ChiTaRS; Cers5; mouse.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050291; F:sphingosine N-acyltransferase activity; IEA:InterPro.
DR   GO; GO:0046513; P:ceramide biosynthetic process; IEA:InterPro.
DR   InterPro; IPR016439; Lag1/Lac1-like.
DR   InterPro; IPR006634; TLC-dom.
DR   PANTHER; PTHR12560:SF8; CERAMIDE SYNTHASE 5; 1.
DR   PANTHER; PTHR12560; LONGEVITY ASSURANCE FACTOR 1 LAG1; 1.
DR   Pfam; PF03798; TRAM_LAG1_CLN8; 1.
DR   PIRSF; PIRSF005225; LAG1_LAC1; 1.
DR   SMART; SM00724; TLC; 1.
DR   PROSITE; PS50922; TLC; 1.
PE   2: Evidence at transcript level;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PROSITE-
KW   ProRule:PRU00205};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|PROSITE-
KW   ProRule:PRU00205};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        68..90
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        110..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          1..139
FT                   /note="TLC"
FT                   /evidence="ECO:0000259|PROSITE:PS50922"
FT   REGION          146..172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        146..164
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   213 AA;  24022 MW;  74B7A2FC112DF430 CRC64;
     MFSQFIDVKR KDFLMMFIHH MIGIMLTTFS YVNNMVRVGA LIFCLHDFAD PLLEAAKMAN
     YARRERLCTT LFVIFGAAFI VSRLAIFPLW ILNTTLFESW EIIGPYPSWW LFNALLLILQ
     VLHAIWSYLI VQTASKALSR GKVSKDDRSD VESSSEEEDE TTHKNNLSGS SSSNGANCMN
     GYMGGSHLAE EQGTCKATGN LHFRASPHLH SCD
//