Q3UJF0 (GPR55_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G-protein coupled receptor 55

Gene names

Name:	Gpr55
Synonyms:	Gm218

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for L-alpha-lysophosphatidylinositol (LPI). LPI induces Ca2+ release from intracellular stores via the heterotrimeric G protein GNA13 and RHOA By similarity. Putative cannabinoid receptor By similarity. May play a role in bone physiology by regulating osteoclast number and function By similarity. May be involved in hyperalgesia associated with inflammatory and neuropathic pain. Ref.3
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Disruption phenotype	Mutant mice are resistant to mechanical hyperalgesia and have increased levels of anti-inflammatory cytokines. Ref.3
Miscellaneous	The classification of this protein as a cannabinoid receptor remains a contentious issue due to conflicting pharmacological results.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of phospholipase C activity Inferred from sequence or structural similarity. Source: UniProtKB bone resorption Inferred from electronic annotation. Source: Compara negative regulation of osteoclast differentiation Inferred from electronic annotation. Source: Compara positive regulation of ERK1 and ERK2 cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of Rho protein signal transduction Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cannabinoid receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	G-protein coupled receptor 55		PRO_0000233978
Regions
	Topological domain	1 – 20	20	Extracellular Potential
	Transmembrane	21 – 41	21	Helical; Name=1; Potential
	Topological domain	42 – 57	16	Cytoplasmic Potential
	Transmembrane	58 – 78	21	Helical; Name=2; Potential
	Topological domain	79 – 93	15	Extracellular Potential
	Transmembrane	94 – 114	21	Helical; Name=3; Potential
	Topological domain	115 – 136	22	Cytoplasmic Potential
	Transmembrane	137 – 157	21	Helical; Name=4; Potential
	Topological domain	158 – 179	22	Extracellular Potential
	Transmembrane	180 – 200	21	Helical; Name=5; Potential
	Topological domain	201 – 239	39	Cytoplasmic Potential
	Transmembrane	240 – 260	21	Helical; Name=6; Potential
	Topological domain	261 – 279	19	Extracellular Potential
	Transmembrane	280 – 300	21	Helical; Name=7; Potential
	Topological domain	301 – 327	27	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	8	1	N-linked (GlcNAc...) Potential
	Glycosylation	170	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	226 – 231	6	WVQKRA → LGTKREP in BAE27205. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q3UJF0 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 4B33F94E3CCAEA1C	FASTA	327	38,090
	10 20 30 40 50 60 MSQPERDNCS FDSVDKLTRT LQLAVHIPTF LLGLVLNLLA IRGFSAFLKK RKLDYIATSI 70 80 90 100 110 120 YMINLAVFDL LLVLSLPFKM VLPQVESPLP SFCTLVECLY FISMYGSVFT ICFISLDRFL 130 140 150 160 170 180 AIQYPILASH LRSPRKTFGI CCIIWMLVWI GSIPIYTFHR EVERYKCFHN MSDVTWSASV 190 200 210 220 230 240 FFPLEIFGFL LPMGIMGFCS YRSIHILLRR PDSTEDWVQQ RDTKGWVQKR ACIWTIATNL 250 260 270 280 290 300 VIFVVSFLPV HLGFFLQYLV RNRFILDCRM KQGISLFLQL SLCFSNINCC LDVFCYYFVI 310 320 KEFRMRIKAH RPSTIKLVNQ DTMVSRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Amnion.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The putative cannabinoid receptor GPR55 plays a role in mechanical hyperalgesia associated with inflammatory and neuropathic pain." Staton P.C., Hatcher J.P., Walker D.J., Morrison A.D., Shapland E.M., Hughes J.P., Chong E., Mander P.K., Green P.J., Billinton A., Fulleylove M., Lancaster H.C., Smith J.C., Bailey L.T., Wise A., Brown A.J., Richardson J.C., Chessell I.P. Pain 139:225-236(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK146484 mRNA. Translation: BAE27205.1. AC102506 Genomic DNA. No translation available. AC107707 Genomic DNA. No translation available.
IPI	IPI00339865. IPI01007981.
RefSeq	NP_001028462.2. NM_001033290.2.
UniGene	Mm.244158.
3D structure databases
ProteinModelPortal	Q3UJF0.
SMR	Q3UJF0. Positions 12-314.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q3UJF0.
Proteomic databases
PRIDE	Q3UJF0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000086975; ENSMUSP00000084196; ENSMUSG00000049608.
GeneID	227326.
KEGG	mmu:227326.
Organism-specific databases
CTD	9290.
MGI	MGI:2685064. Gpr55.
Phylogenomic databases
eggNOG	NOG145477.
GeneTree	ENSGT00530000063922.
HOGENOM	HOG000013155.
HOVERGEN	HBG096388.
InParanoid	Q3UJF0.
KO	K08413.
OMA	CFHNMSD.
OrthoDB	EOG4JT063.
Gene expression databases
CleanEx	MM_GPR55.
Genevestigator	Q3UJF0.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

GPR55_MOUSE

Accession

Primary (citable) accession number: Q3UJF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program