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Protein

Ubiquitin carboxyl-terminal hydrolase 19

Gene

Usp19

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme that regulates the degradation of various proteins. Deubiquitinates and prevents proteasomal degradation of RNF123 which in turn stimulates CDKN1B ubiquitin-dependent degradation thereby playing a role in cell proliferation. Involved in decreased protein synthesis in atrophying skeletal muscle. Modulates transcription of major myofibrillar proteins. Also involved in turnover of endoplasmic-reticulum-associated degradation (ERAD) substrates (By similarity). Regulates the stability of BIRC2/c-IAP1 and BIRC3/c-IAP2 by preventing thier ubiquitination. Required for cells to mount an appropriate response to hypoxia and rescues HIF1A from degradation in a non-catalytic manner. Exhibits a preference towards 'Lys-63'-linked Ubiquitin chains (By similarity). Plays an important role in 17 beta-estradiol (E2)-inhibited myogenesis. Decreases the levels of ubiquitinated proteins during skeletal muscle formation and acts to repress myogenesis.By similarity1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei548NucleophilePROSITE-ProRule annotation1
Active sitei1207Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri833 – 875MYND-typePROSITE-ProRule annotationAdd BLAST43

GO - Molecular functioni

  • Hsp90 protein binding Source: MGI
  • Lys48-specific deubiquitinase activity Source: ParkinsonsUK-UCL
  • metal ion binding Source: UniProtKB-KW
  • thiol-dependent ubiquitin-specific protease activity Source: UniProtKB
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.024.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 19 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 19
Ubiquitin thioesterase 19
Ubiquitin-specific-processing protease 19
Gene namesi
Name:Usp19
Synonyms:Kiaa0891
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1918722. Usp19.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 1333CytoplasmicSequence analysisAdd BLAST1333
Transmembranei1334 – 1354HelicalSequence analysisAdd BLAST21
Topological domaini1355 – 1360LumenalSequence analysis6

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951571 – 1360Ubiquitin carboxyl-terminal hydrolase 19Add BLAST1360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei220PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UJD6.
PaxDbiQ3UJD6.
PeptideAtlasiQ3UJD6.
PRIDEiQ3UJD6.

PTM databases

iPTMnetiQ3UJD6.
PhosphoSitePlusiQ3UJD6.
SwissPalmiQ3UJD6.

Expressioni

Inductioni

Up-regulated by ESR1 in the presence of 17 beta-estradiol (E2).1 Publication

Gene expression databases

BgeeiENSMUSG00000006676.
CleanExiMM_USP19.
ExpressionAtlasiQ3UJD6. baseline and differential.
GenevisibleiQ3UJD6. MM.

Interactioni

Subunit structurei

Interacts with and stabilizes RNF123 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts with HIF1A (via N-terminus) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
FKBP6O753442EBI-9359023,EBI-744771From a different organism.

GO - Molecular functioni

  • Hsp90 protein binding Source: MGI
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi214730. 3 interactors.
IntActiQ3UJD6. 26 interactors.
STRINGi10090.ENSMUSP00000006854.

Structurei

3D structure databases

ProteinModelPortaliQ3UJD6.
SMRiQ3UJD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 140CS 1PROSITE-ProRule annotationAdd BLAST90
Domaini322 – 424CS 2PROSITE-ProRule annotationAdd BLAST103
Domaini539 – 1256USPAdd BLAST718

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi524 – 530Poly-Glu7

Sequence similaritiesi

Contains 2 CS domains.PROSITE-ProRule annotation
Contains 1 MYND-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri833 – 875MYND-typePROSITE-ProRule annotationAdd BLAST43

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG1870. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000074206.
HOVERGENiHBG061889.
InParanoidiQ3UJD6.
KOiK11847.
OMAiAMNAHTP.
OrthoDBiEOG091G021E.
PhylomeDBiQ3UJD6.
TreeFamiTF106276.

Family and domain databases

Gene3Di2.60.40.790. 2 hits.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR002893. Znf_MYND.
[Graphical view]
PfamiPF04969. CS. 2 hits.
PF00443. UCH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 2 hits.
PROSITEiPS51203. CS. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UJD6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAGASATGP RRGPPGLEEA TSKKKQKDRA NLESKDGDAR RVSLPRKEPT
60 70 80 90 100
KDELLLDWRQ SADEVIVKLR VGTGPVRLED VDAAFTDTDC VVRLPDGRQW
110 120 130 140 150
GGVFFAEIQS SCTKVQARKG GLLQLVLPKK VPLLTWPSLL KPLGTQELVP
160 170 180 190 200
GLQCQENGQE LSPIALEPGS EPRRAKQEAR NQKRAQGRGE VGSGAGPGTQ
210 220 230 240 250
AGPSAKRAVH LRRGPEGEGS MDGPGPQGDA PSFLSDSATQ VEAEEKLCAP
260 270 280 290 300
PMNTQTSLLS SEKSLALLTV EKTVSPRNDP VAPVMVQDRD PEPEQEDQVK
310 320 330 340 350
EEMALGADPT ALVEEPESMV NLAFVKNDSY EKGPDSVVVH VYVKEIRRDS
360 370 380 390 400
SRVLFREQDF TLIFQTRDGN FLRLHPGCGP HTIFRWQVKL RNLIEPEQCT
410 420 430 440 450
FCFTASRIDI CLRKRQSQRW GGLEAPATRG AVGGAKVAVP TGPTPLDSTP
460 470 480 490 500
PGGGPHPLTG QEEARAVEKE KPKARSEDSG LDGVVARTPL EHVAPKPDPH
510 520 530 540 550
LASPKPTCMV PPMPHSPVSG DSVEEDEEEE KKVCLPGFTG LVNLGNTCFM
560 570 580 590 600
NSVIQSLSNT RELRDFFHDR SFEAEINYNN PLGTGGRLAI GFAVLLRALW
610 620 630 640 650
KGTHQAFQPS KLKAIVASKA SQFTGYAQHD AQEFMAFLLD GLHEDLNRIQ
660 670 680 690 700
NKPYTETVDS DGRPDEVVAE EAWQRHKMRN DSFIVDLFQG QYKSKLVCPV
710 720 730 740 750
CAKVSITFDP FLYLPVPLPQ KQKVLPIFYF AREPHSKPIK FLVSVSKENS
760 770 780 790 800
SASEVLDSLS QSVHVKPENL RLAEVIKNRF HRVFLPSHSL DAVSPTDVLL
810 820 830 840 850
CFELLSPELA KERVVVLEVQ QRPQVPSIPI SKCAACQRKQ QSEEEKLKRC
860 870 880 890 900
TRCYRVGYCN QFCQKTHWPD HKGLCRPENI GYPFLVSVPA SRLTYARLAQ
910 920 930 940 950
LLEGYARYSV SVFQPPFQPG RMALESQSPG CTTLLSTSSL EAGDSEREPI
960 970 980 990 1000
QPSELQLVTP VAEGDTGAHR VWPPADRGPV PSTSGLSSEM LASGPIEGCP
1010 1020 1030 1040 1050
LLAGERVSRP EAAVPGYQHS SESVNTHTPQ FFIYKIDASN REQRLEDKGE
1060 1070 1080 1090 1100
TPLELGDDCS LALVWRNNER LQEFVLVASK ELECAEDPGS AGEAARAGHF
1110 1120 1130 1140 1150
TLDQCLNLFT RPEVLAPEEA WYCPQCKQHR EASKQLLLWR LPNVLIVQLK
1160 1170 1180 1190 1200
RFSFRSFIWR DKINDLVEFP VRNLDLSKFC IGQKEEQLPS YDLYAVINHY
1210 1220 1230 1240 1250
GGMIGGHYTA CARLPNDRSS QRSDVGWRLF DDSTVTTVDE SQVVTRYAYV
1260 1270 1280 1290 1300
LFYRRRNSPV ERPPRASHSE HHPDLGPAAE AAASQASRIW QELEAEEEMV
1310 1320 1330 1340 1350
PEGPGPLGPW GPQDWVGPPP RGPTTPDEGC LRYFVLGTVA ALVALVLNVF
1360
YPLVSQSRWR
Length:1,360
Mass (Da):150,549
Last modified:October 11, 2005 - v1
Checksum:iFE9C3020D2CD9AED
GO
Isoform 2 (identifier: Q3UJD6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1286-1340: ASRIWQELEA...LRYFVLGTVA → GLGPGQAPEV...PTYSNMEEVD
     1341-1360: Missing.

Show »
Length:1,323
Mass (Da):146,283
Checksum:i73E85AA9778738F7
GO

Sequence cautioni

The sequence BAC65678 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140L → LK in BAE42739 (PubMed:16141072).Curated1
Sequence conflicti142P → S in AAH60613 (PubMed:15489334).Curated1
Sequence conflicti430 – 431Missing in BAE42739 (PubMed:16141072).Curated2
Sequence conflicti669A → T in BAE42739 (PubMed:16141072).Curated1
Sequence conflicti1058D → E in BAE42739 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0267691286 – 1340ASRIW…LGTVA → GLGPGQAPEVAPTRTAPERF APPVDRPAPTYSNMEEVD in isoform 2. 3 PublicationsAdd BLAST55
Alternative sequenceiVSP_0267701341 – 1360Missing in isoform 2. 3 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122396 mRNA. Translation: BAC65678.4. Different initiation.
AK146504 mRNA. Translation: BAE27219.1.
AK160807 mRNA. Translation: BAE36025.1.
AK171942 mRNA. Translation: BAE42739.1.
BC046824 mRNA. Translation: AAH46824.1.
BC060613 mRNA. Translation: AAH60613.1.
CCDSiCCDS23529.1. [Q3UJD6-1]
CCDS52924.1. [Q3UJD6-2]
RefSeqiNP_001161843.1. NM_001168371.2.
NP_001161844.1. NM_001168372.2. [Q3UJD6-2]
NP_001161845.1. NM_001168373.2.
NP_082080.3. NM_027804.4. [Q3UJD6-1]
NP_663382.2. NM_145407.3.
UniGeneiMm.289706.

Genome annotation databases

EnsembliENSMUST00000006854; ENSMUSP00000006854; ENSMUSG00000006676. [Q3UJD6-1]
ENSMUST00000085044; ENSMUSP00000082119; ENSMUSG00000006676. [Q3UJD6-2]
GeneIDi71472.
KEGGimmu:71472.
UCSCiuc009rpt.2. mouse. [Q3UJD6-1]
uc009rpv.2. mouse. [Q3UJD6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122396 mRNA. Translation: BAC65678.4. Different initiation.
AK146504 mRNA. Translation: BAE27219.1.
AK160807 mRNA. Translation: BAE36025.1.
AK171942 mRNA. Translation: BAE42739.1.
BC046824 mRNA. Translation: AAH46824.1.
BC060613 mRNA. Translation: AAH60613.1.
CCDSiCCDS23529.1. [Q3UJD6-1]
CCDS52924.1. [Q3UJD6-2]
RefSeqiNP_001161843.1. NM_001168371.2.
NP_001161844.1. NM_001168372.2. [Q3UJD6-2]
NP_001161845.1. NM_001168373.2.
NP_082080.3. NM_027804.4. [Q3UJD6-1]
NP_663382.2. NM_145407.3.
UniGeneiMm.289706.

3D structure databases

ProteinModelPortaliQ3UJD6.
SMRiQ3UJD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214730. 3 interactors.
IntActiQ3UJD6. 26 interactors.
STRINGi10090.ENSMUSP00000006854.

Protein family/group databases

MEROPSiC19.024.

PTM databases

iPTMnetiQ3UJD6.
PhosphoSitePlusiQ3UJD6.
SwissPalmiQ3UJD6.

Proteomic databases

MaxQBiQ3UJD6.
PaxDbiQ3UJD6.
PeptideAtlasiQ3UJD6.
PRIDEiQ3UJD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006854; ENSMUSP00000006854; ENSMUSG00000006676. [Q3UJD6-1]
ENSMUST00000085044; ENSMUSP00000082119; ENSMUSG00000006676. [Q3UJD6-2]
GeneIDi71472.
KEGGimmu:71472.
UCSCiuc009rpt.2. mouse. [Q3UJD6-1]
uc009rpv.2. mouse. [Q3UJD6-2]

Organism-specific databases

CTDi10869.
MGIiMGI:1918722. Usp19.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1870. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000074206.
HOVERGENiHBG061889.
InParanoidiQ3UJD6.
KOiK11847.
OMAiAMNAHTP.
OrthoDBiEOG091G021E.
PhylomeDBiQ3UJD6.
TreeFamiTF106276.

Enzyme and pathway databases

ReactomeiR-MMU-5689880. Ub-specific processing proteases.

Miscellaneous databases

ChiTaRSiUsp19. mouse.
PROiQ3UJD6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006676.
CleanExiMM_USP19.
ExpressionAtlasiQ3UJD6. baseline and differential.
GenevisibleiQ3UJD6. MM.

Family and domain databases

Gene3Di2.60.40.790. 2 hits.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR002893. Znf_MYND.
[Graphical view]
PfamiPF04969. CS. 2 hits.
PF00443. UCH. 1 hit.
PF01753. zf-MYND. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 2 hits.
PROSITEiPS51203. CS. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP19_MOUSE
AccessioniPrimary (citable) accession number: Q3UJD6
Secondary accession number(s): Q3TAC9
, Q3TUE6, Q6P9T0, Q80TP5, Q80ZW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.