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Q3UIZ8

- MYLK3_MOUSE

UniProt

Q3UIZ8 - MYLK3_MOUSE

Protein

Myosin light chain kinase 3

Gene

Mylk3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.2 Publications

    Catalytic activityi

    ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

    Cofactori

    Magnesium.By similarity

    Kineticsi

    1. KM=4.3 µM for MYL21 Publication

    Vmax=0.26 µmol/min/mg enzyme1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei520 – 5201ATPPROSITE-ProRule annotation
    Active sitei612 – 6121Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi497 – 5059ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calmodulin-dependent protein kinase activity Source: BHF-UCL
    3. myosin light chain kinase activity Source: BHF-UCL

    GO - Biological processi

    1. cardiac myofibril assembly Source: Ensembl
    2. cellular response to interleukin-1 Source: Ensembl
    3. positive regulation of sarcomere organization Source: Ensembl
    4. protein phosphorylation Source: BHF-UCL
    5. regulation of vascular permeability involved in acute inflammatory response Source: Ensembl
    6. sarcomere organization Source: BHF-UCL
    7. sarcomerogenesis Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin light chain kinase 3 (EC:2.7.11.18)
    Alternative name(s):
    Cardiac-MyBP-C-associated Ca/CaM kinase
    Short name:
    Cardiac-MLCK
    Gene namesi
    Name:Mylk3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:2443063. Mylk3.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: BHF-UCL
    2. cytosol Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 795795Myosin light chain kinase 3PRO_0000272201Add
    BLAST

    Post-translational modificationi

    Phosphorylated on serine residues.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ3UIZ8.
    PaxDbiQ3UIZ8.
    PRIDEiQ3UIZ8.

    PTM databases

    PhosphoSiteiQ3UIZ8.

    Expressioni

    Tissue specificityi

    Restricted to cardiomyocytes (at protein level). Down-regulated in heart after experimental myocardial infarction at the protein level; no significant changes at the mRNA level.1 Publication

    Developmental stagei

    Up-regulated in the heart from 10.5 dpc to neonates and further increased in adults. Down-regulated in aged hearts (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiQ3UIZ8.
    BgeeiQ3UIZ8.
    CleanExiMM_MYLK3.
    GenevestigatoriQ3UIZ8.

    Interactioni

    Protein-protein interaction databases

    IntActiQ3UIZ8. 1 interaction.
    MINTiMINT-4112993.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3UIZ8.
    SMRiQ3UIZ8. Positions 429-765.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini491 – 746256Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117629.
    HOGENOMiHOG000233016.
    HOVERGENiHBG080416.
    InParanoidiQ3UIZ8.
    KOiK00907.
    OMAiFRMVVAV.
    OrthoDBiEOG73FQMV.
    PhylomeDBiQ3UIZ8.
    TreeFamiTF314166.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3UIZ8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSGVSEEDPE GLAPQGLPAL GGACLATMDK KLNVLTEKVD RLLHFQEDVT    50
    EKLQCVCQGM DHLEQDLHRL EASRELSLAG SGSTPPTTAQ AAWPEVLELV 100
    RAVRQEGAQH GARLEALFKM VVAVDRAITL VGSTFQNSKV ADFIMQGTVP 150
    GRKGSLADGP EENKEQAEVA GVKPNHVLTT GGVQADASRT LWEESQKEDI 200
    PVRTVEGLPL IINTSLKGAD LTQAGASLRQ GVEVLGPGQV PLPTEAESRL 250
    PETASENTGA TLELSVAIDR ISEVLTSLKM SQGGGQETSS SKPDCWLSEE 300
    AMRLSSGPLP QPLGPLTPDS DIHSGDALPR IPINMQEMAT PGELLETQSG 350
    SPIGSAEAPG LGTVLEDQIP KGARPFPPLP KRSSNNGGMS AEEEIGSGAE 400
    PMRGPSLATR DWRDETVGTT DLQQGIDPGA VSPEPGKDHA AQGPGRTEAG 450
    RLSSAAEAAI VVLDDSAAPP APFEHRVVSI KDTLISAGYT VSQHEVLGGG 500
    RFGQVHRCTE RSTGLALAAK IIKVKNVKDR EDVKNEVNIM NQLSHVNLIQ 550
    LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVV LFTRQICEGV 600
    HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFG 650
    TPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI 700
    VNCSWDFDAD TFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP 750
    AKASGSNVRL RSQQLLQKYM AQSKWKKHFH VVTAVNRLRK FPTCP 795
    Length:795
    Mass (Da):86,372
    Last modified:October 11, 2005 - v1
    Checksum:i9B9874D50E6EBA20
    GO
    Isoform 2 (identifier: Q3UIZ8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         100-162: Missing.
         777-795: KHFHVVTAVNRLRKFPTCP → VFWVFFSKSCI

    Note: No experimental confirmation available.

    Show »
    Length:724
    Mass (Da):78,790
    Checksum:iAE80A5AB899A23C4
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei100 – 16263Missing in isoform 2. 1 PublicationVSP_022369Add
    BLAST
    Alternative sequencei777 – 79519KHFHV…FPTCP → VFWVFFSKSCI in isoform 2. 1 PublicationVSP_022370Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU403565 mRNA. Translation: ABY89726.1.
    AK031546 mRNA. Translation: BAE43277.1.
    AK052858 mRNA. Translation: BAC35177.1.
    AK146683 mRNA. Translation: BAE27357.1.
    CCDSiCCDS40422.1. [Q3UIZ8-1]
    RefSeqiNP_780650.2. NM_175441.5. [Q3UIZ8-1]
    UniGeneiMm.32804.

    Genome annotation databases

    EnsembliENSMUST00000034133; ENSMUSP00000034133; ENSMUSG00000031698. [Q3UIZ8-1]
    ENSMUST00000121972; ENSMUSP00000113960; ENSMUSG00000031698. [Q3UIZ8-2]
    GeneIDi213435.
    KEGGimmu:213435.
    UCSCiuc009mps.1. mouse. [Q3UIZ8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EU403565 mRNA. Translation: ABY89726.1 .
    AK031546 mRNA. Translation: BAE43277.1 .
    AK052858 mRNA. Translation: BAC35177.1 .
    AK146683 mRNA. Translation: BAE27357.1 .
    CCDSi CCDS40422.1. [Q3UIZ8-1 ]
    RefSeqi NP_780650.2. NM_175441.5. [Q3UIZ8-1 ]
    UniGenei Mm.32804.

    3D structure databases

    ProteinModelPortali Q3UIZ8.
    SMRi Q3UIZ8. Positions 429-765.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q3UIZ8. 1 interaction.
    MINTi MINT-4112993.

    PTM databases

    PhosphoSitei Q3UIZ8.

    Proteomic databases

    MaxQBi Q3UIZ8.
    PaxDbi Q3UIZ8.
    PRIDEi Q3UIZ8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034133 ; ENSMUSP00000034133 ; ENSMUSG00000031698 . [Q3UIZ8-1 ]
    ENSMUST00000121972 ; ENSMUSP00000113960 ; ENSMUSG00000031698 . [Q3UIZ8-2 ]
    GeneIDi 213435.
    KEGGi mmu:213435.
    UCSCi uc009mps.1. mouse. [Q3UIZ8-1 ]

    Organism-specific databases

    CTDi 91807.
    MGIi MGI:2443063. Mylk3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117629.
    HOGENOMi HOG000233016.
    HOVERGENi HBG080416.
    InParanoidi Q3UIZ8.
    KOi K00907.
    OMAi FRMVVAV.
    OrthoDBi EOG73FQMV.
    PhylomeDBi Q3UIZ8.
    TreeFami TF314166.

    Miscellaneous databases

    NextBioi 373954.
    PROi Q3UIZ8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q3UIZ8.
    Bgeei Q3UIZ8.
    CleanExi MM_MYLK3.
    Genevestigatori Q3UIZ8.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION.
      Tissue: Heart.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Heart and Testis.
    3. Cited for: FUNCTION.

    Entry informationi

    Entry nameiMYLK3_MOUSE
    AccessioniPrimary (citable) accession number: Q3UIZ8
    Secondary accession number(s): B0LY41, Q3V3V0, Q8BWD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2007
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3