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Q3UIZ8

- MYLK3_MOUSE

UniProt

Q3UIZ8 - MYLK3_MOUSE

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Protein

Myosin light chain kinase 3

Gene
Mylk3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (1 Publication), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (1 Publication). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.2 Publications

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Cofactori

Magnesium By similarity.

Kineticsi

  1. KM=4.3 µM for MYL21 Publication

Vmax=0.26 µmol/min/mg enzyme

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei520 – 5201ATP By similarity
Active sitei612 – 6121Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi497 – 5059ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin-dependent protein kinase activity Source: BHF-UCL
  3. myosin light chain kinase activity Source: BHF-UCL

GO - Biological processi

  1. cardiac myofibril assembly Source: Ensembl
  2. cellular response to interleukin-1 Source: Ensembl
  3. positive regulation of sarcomere organization Source: Ensembl
  4. protein phosphorylation Source: BHF-UCL
  5. regulation of vascular permeability involved in acute inflammatory response Source: Ensembl
  6. sarcomere organization Source: BHF-UCL
  7. sarcomerogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 3 (EC:2.7.11.18)
Alternative name(s):
Cardiac-MyBP-C-associated Ca/CaM kinase
Short name:
Cardiac-MLCK
Gene namesi
Name:Mylk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:2443063. Mylk3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: BHF-UCL
  2. cytosol Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 795795Myosin light chain kinase 3PRO_0000272201Add
BLAST

Post-translational modificationi

Phosphorylated on serine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UIZ8.
PaxDbiQ3UIZ8.
PRIDEiQ3UIZ8.

PTM databases

PhosphoSiteiQ3UIZ8.

Expressioni

Tissue specificityi

Restricted to cardiomyocytes (at protein level). Down-regulated in heart after experimental myocardial infarction at the protein level; no significant changes at the mRNA level.1 Publication

Developmental stagei

Up-regulated in the heart from 10.5 dpc to neonates and further increased in adults. Down-regulated in aged hearts (at protein level).1 Publication

Gene expression databases

ArrayExpressiQ3UIZ8.
BgeeiQ3UIZ8.
CleanExiMM_MYLK3.
GenevestigatoriQ3UIZ8.

Interactioni

Protein-protein interaction databases

IntActiQ3UIZ8. 1 interaction.
MINTiMINT-4112993.

Structurei

3D structure databases

ProteinModelPortaliQ3UIZ8.
SMRiQ3UIZ8. Positions 429-765.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini491 – 746256Protein kinaseAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117629.
HOGENOMiHOG000233016.
HOVERGENiHBG080416.
InParanoidiQ3UIZ8.
KOiK00907.
OMAiFRMVVAV.
OrthoDBiEOG73FQMV.
PhylomeDBiQ3UIZ8.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3UIZ8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSGVSEEDPE GLAPQGLPAL GGACLATMDK KLNVLTEKVD RLLHFQEDVT    50
EKLQCVCQGM DHLEQDLHRL EASRELSLAG SGSTPPTTAQ AAWPEVLELV 100
RAVRQEGAQH GARLEALFKM VVAVDRAITL VGSTFQNSKV ADFIMQGTVP 150
GRKGSLADGP EENKEQAEVA GVKPNHVLTT GGVQADASRT LWEESQKEDI 200
PVRTVEGLPL IINTSLKGAD LTQAGASLRQ GVEVLGPGQV PLPTEAESRL 250
PETASENTGA TLELSVAIDR ISEVLTSLKM SQGGGQETSS SKPDCWLSEE 300
AMRLSSGPLP QPLGPLTPDS DIHSGDALPR IPINMQEMAT PGELLETQSG 350
SPIGSAEAPG LGTVLEDQIP KGARPFPPLP KRSSNNGGMS AEEEIGSGAE 400
PMRGPSLATR DWRDETVGTT DLQQGIDPGA VSPEPGKDHA AQGPGRTEAG 450
RLSSAAEAAI VVLDDSAAPP APFEHRVVSI KDTLISAGYT VSQHEVLGGG 500
RFGQVHRCTE RSTGLALAAK IIKVKNVKDR EDVKNEVNIM NQLSHVNLIQ 550
LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVV LFTRQICEGV 600
HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFG 650
TPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI 700
VNCSWDFDAD TFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP 750
AKASGSNVRL RSQQLLQKYM AQSKWKKHFH VVTAVNRLRK FPTCP 795
Length:795
Mass (Da):86,372
Last modified:October 11, 2005 - v1
Checksum:i9B9874D50E6EBA20
GO
Isoform 2 (identifier: Q3UIZ8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-162: Missing.
     777-795: KHFHVVTAVNRLRKFPTCP → VFWVFFSKSCI

Note: No experimental confirmation available.

Show »
Length:724
Mass (Da):78,790
Checksum:iAE80A5AB899A23C4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei100 – 16263Missing in isoform 2. VSP_022369Add
BLAST
Alternative sequencei777 – 79519KHFHV…FPTCP → VFWVFFSKSCI in isoform 2. VSP_022370Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU403565 mRNA. Translation: ABY89726.1.
AK031546 mRNA. Translation: BAE43277.1.
AK052858 mRNA. Translation: BAC35177.1.
AK146683 mRNA. Translation: BAE27357.1.
CCDSiCCDS40422.1. [Q3UIZ8-1]
RefSeqiNP_780650.2. NM_175441.5. [Q3UIZ8-1]
UniGeneiMm.32804.

Genome annotation databases

EnsembliENSMUST00000034133; ENSMUSP00000034133; ENSMUSG00000031698. [Q3UIZ8-1]
ENSMUST00000121972; ENSMUSP00000113960; ENSMUSG00000031698. [Q3UIZ8-2]
GeneIDi213435.
KEGGimmu:213435.
UCSCiuc009mps.1. mouse. [Q3UIZ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU403565 mRNA. Translation: ABY89726.1 .
AK031546 mRNA. Translation: BAE43277.1 .
AK052858 mRNA. Translation: BAC35177.1 .
AK146683 mRNA. Translation: BAE27357.1 .
CCDSi CCDS40422.1. [Q3UIZ8-1 ]
RefSeqi NP_780650.2. NM_175441.5. [Q3UIZ8-1 ]
UniGenei Mm.32804.

3D structure databases

ProteinModelPortali Q3UIZ8.
SMRi Q3UIZ8. Positions 429-765.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3UIZ8. 1 interaction.
MINTi MINT-4112993.

PTM databases

PhosphoSitei Q3UIZ8.

Proteomic databases

MaxQBi Q3UIZ8.
PaxDbi Q3UIZ8.
PRIDEi Q3UIZ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034133 ; ENSMUSP00000034133 ; ENSMUSG00000031698 . [Q3UIZ8-1 ]
ENSMUST00000121972 ; ENSMUSP00000113960 ; ENSMUSG00000031698 . [Q3UIZ8-2 ]
GeneIDi 213435.
KEGGi mmu:213435.
UCSCi uc009mps.1. mouse. [Q3UIZ8-1 ]

Organism-specific databases

CTDi 91807.
MGIi MGI:2443063. Mylk3.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117629.
HOGENOMi HOG000233016.
HOVERGENi HBG080416.
InParanoidi Q3UIZ8.
KOi K00907.
OMAi FRMVVAV.
OrthoDBi EOG73FQMV.
PhylomeDBi Q3UIZ8.
TreeFami TF314166.

Miscellaneous databases

NextBioi 373954.
PROi Q3UIZ8.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q3UIZ8.
Bgeei Q3UIZ8.
CleanExi MM_MYLK3.
Genevestigatori Q3UIZ8.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION.
    Tissue: Heart.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Heart and Testis.
  3. Cited for: FUNCTION.

Entry informationi

Entry nameiMYLK3_MOUSE
AccessioniPrimary (citable) accession number: Q3UIZ8
Secondary accession number(s): B0LY41, Q3V3V0, Q8BWD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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