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Q3UIZ8

- MYLK3_MOUSE

UniProt

Q3UIZ8 - MYLK3_MOUSE

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Protein

Myosin light chain kinase 3

Gene

Mylk3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.2 Publications

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Cofactori

Magnesium.By similarity

Kineticsi

  1. KM=4.3 µM for MYL21 Publication

Vmax=0.26 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei520 – 5201ATPPROSITE-ProRule annotation
Active sitei612 – 6121Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi497 – 5059ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calmodulin-dependent protein kinase activity Source: BHF-UCL
  3. myosin light chain kinase activity Source: BHF-UCL

GO - Biological processi

  1. cardiac myofibril assembly Source: Ensembl
  2. cellular response to interleukin-1 Source: Ensembl
  3. positive regulation of sarcomere organization Source: Ensembl
  4. protein phosphorylation Source: BHF-UCL
  5. regulation of vascular permeability involved in acute inflammatory response Source: Ensembl
  6. sarcomere organization Source: BHF-UCL
  7. sarcomerogenesis Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 3 (EC:2.7.11.18)
Alternative name(s):
Cardiac-MyBP-C-associated Ca/CaM kinase
Short name:
Cardiac-MLCK
Gene namesi
Name:Mylk3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:2443063. Mylk3.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: BHF-UCL
  2. cytosol Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 795795Myosin light chain kinase 3PRO_0000272201Add
BLAST

Post-translational modificationi

Phosphorylated on serine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UIZ8.
PaxDbiQ3UIZ8.
PRIDEiQ3UIZ8.

PTM databases

PhosphoSiteiQ3UIZ8.

Expressioni

Tissue specificityi

Restricted to cardiomyocytes (at protein level). Down-regulated in heart after experimental myocardial infarction at the protein level; no significant changes at the mRNA level.1 Publication

Developmental stagei

Up-regulated in the heart from 10.5 dpc to neonates and further increased in adults. Down-regulated in aged hearts (at protein level).1 Publication

Gene expression databases

BgeeiQ3UIZ8.
CleanExiMM_MYLK3.
ExpressionAtlasiQ3UIZ8. baseline and differential.
GenevestigatoriQ3UIZ8.

Interactioni

Protein-protein interaction databases

IntActiQ3UIZ8. 1 interaction.
MINTiMINT-4112993.

Structurei

3D structure databases

ProteinModelPortaliQ3UIZ8.
SMRiQ3UIZ8. Positions 429-765.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini491 – 746256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118877.
HOGENOMiHOG000233016.
HOVERGENiHBG080416.
InParanoidiQ3UIZ8.
KOiK00907.
OMAiFRMVVAV.
OrthoDBiEOG73FQMV.
PhylomeDBiQ3UIZ8.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3UIZ8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGVSEEDPE GLAPQGLPAL GGACLATMDK KLNVLTEKVD RLLHFQEDVT
60 70 80 90 100
EKLQCVCQGM DHLEQDLHRL EASRELSLAG SGSTPPTTAQ AAWPEVLELV
110 120 130 140 150
RAVRQEGAQH GARLEALFKM VVAVDRAITL VGSTFQNSKV ADFIMQGTVP
160 170 180 190 200
GRKGSLADGP EENKEQAEVA GVKPNHVLTT GGVQADASRT LWEESQKEDI
210 220 230 240 250
PVRTVEGLPL IINTSLKGAD LTQAGASLRQ GVEVLGPGQV PLPTEAESRL
260 270 280 290 300
PETASENTGA TLELSVAIDR ISEVLTSLKM SQGGGQETSS SKPDCWLSEE
310 320 330 340 350
AMRLSSGPLP QPLGPLTPDS DIHSGDALPR IPINMQEMAT PGELLETQSG
360 370 380 390 400
SPIGSAEAPG LGTVLEDQIP KGARPFPPLP KRSSNNGGMS AEEEIGSGAE
410 420 430 440 450
PMRGPSLATR DWRDETVGTT DLQQGIDPGA VSPEPGKDHA AQGPGRTEAG
460 470 480 490 500
RLSSAAEAAI VVLDDSAAPP APFEHRVVSI KDTLISAGYT VSQHEVLGGG
510 520 530 540 550
RFGQVHRCTE RSTGLALAAK IIKVKNVKDR EDVKNEVNIM NQLSHVNLIQ
560 570 580 590 600
LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVV LFTRQICEGV
610 620 630 640 650
HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFG
660 670 680 690 700
TPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI
710 720 730 740 750
VNCSWDFDAD TFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP
760 770 780 790
AKASGSNVRL RSQQLLQKYM AQSKWKKHFH VVTAVNRLRK FPTCP
Length:795
Mass (Da):86,372
Last modified:October 11, 2005 - v1
Checksum:i9B9874D50E6EBA20
GO
Isoform 2 (identifier: Q3UIZ8-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-162: Missing.
     777-795: KHFHVVTAVNRLRKFPTCP → VFWVFFSKSCI

Note: No experimental confirmation available.

Show »
Length:724
Mass (Da):78,790
Checksum:iAE80A5AB899A23C4
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei100 – 16263Missing in isoform 2. 1 PublicationVSP_022369Add
BLAST
Alternative sequencei777 – 79519KHFHV…FPTCP → VFWVFFSKSCI in isoform 2. 1 PublicationVSP_022370Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU403565 mRNA. Translation: ABY89726.1.
AK031546 mRNA. Translation: BAE43277.1.
AK052858 mRNA. Translation: BAC35177.1.
AK146683 mRNA. Translation: BAE27357.1.
CCDSiCCDS40422.1. [Q3UIZ8-1]
RefSeqiNP_001284541.1. NM_001297612.1.
NP_780650.2. NM_175441.5. [Q3UIZ8-1]
UniGeneiMm.32804.

Genome annotation databases

EnsembliENSMUST00000034133; ENSMUSP00000034133; ENSMUSG00000031698. [Q3UIZ8-1]
ENSMUST00000121972; ENSMUSP00000113960; ENSMUSG00000031698. [Q3UIZ8-2]
GeneIDi213435.
KEGGimmu:213435.
UCSCiuc009mps.1. mouse. [Q3UIZ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
EU403565 mRNA. Translation: ABY89726.1 .
AK031546 mRNA. Translation: BAE43277.1 .
AK052858 mRNA. Translation: BAC35177.1 .
AK146683 mRNA. Translation: BAE27357.1 .
CCDSi CCDS40422.1. [Q3UIZ8-1 ]
RefSeqi NP_001284541.1. NM_001297612.1.
NP_780650.2. NM_175441.5. [Q3UIZ8-1 ]
UniGenei Mm.32804.

3D structure databases

ProteinModelPortali Q3UIZ8.
SMRi Q3UIZ8. Positions 429-765.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3UIZ8. 1 interaction.
MINTi MINT-4112993.

PTM databases

PhosphoSitei Q3UIZ8.

Proteomic databases

MaxQBi Q3UIZ8.
PaxDbi Q3UIZ8.
PRIDEi Q3UIZ8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034133 ; ENSMUSP00000034133 ; ENSMUSG00000031698 . [Q3UIZ8-1 ]
ENSMUST00000121972 ; ENSMUSP00000113960 ; ENSMUSG00000031698 . [Q3UIZ8-2 ]
GeneIDi 213435.
KEGGi mmu:213435.
UCSCi uc009mps.1. mouse. [Q3UIZ8-1 ]

Organism-specific databases

CTDi 91807.
MGIi MGI:2443063. Mylk3.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118877.
HOGENOMi HOG000233016.
HOVERGENi HBG080416.
InParanoidi Q3UIZ8.
KOi K00907.
OMAi FRMVVAV.
OrthoDBi EOG73FQMV.
PhylomeDBi Q3UIZ8.
TreeFami TF314166.

Miscellaneous databases

NextBioi 373954.
PROi Q3UIZ8.
SOURCEi Search...

Gene expression databases

Bgeei Q3UIZ8.
CleanExi MM_MYLK3.
ExpressionAtlasi Q3UIZ8. baseline and differential.
Genevestigatori Q3UIZ8.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION.
    Tissue: Heart.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Heart and Testis.
  3. Cited for: FUNCTION.

Entry informationi

Entry nameiMYLK3_MOUSE
AccessioniPrimary (citable) accession number: Q3UIZ8
Secondary accession number(s): B0LY41, Q3V3V0, Q8BWD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: October 11, 2005
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3