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Protein

E3 ubiquitin-protein ligase DTX3L

Gene

Dtx3l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation (By similarity). In concert with PARP9, plays a role in PARP1-dependent DNA damage repair. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri569 – 60840RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase DTX3L (EC:6.3.2.-)
Alternative name(s):
Protein deltex-3-like
Gene namesi
Name:Dtx3l
Synonyms:Bbap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2656973. Dtx3l.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Localizes at sites of DNA damage in a PARP1-dependent manner.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 748747E3 ubiquitin-protein ligase DTX3LPRO_0000390470Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei9 – 91PhosphoserineBy similarity
Modified residuei215 – 2151PhosphoserineBy similarity
Modified residuei536 – 5361PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3UIR3.
MaxQBiQ3UIR3.
PaxDbiQ3UIR3.
PeptideAtlasiQ3UIR3.
PRIDEiQ3UIR3.

PTM databases

iPTMnetiQ3UIR3.
PhosphoSiteiQ3UIR3.

Expressioni

Developmental stagei

Developmentally regulated. Expressed prominently in the thymus and specific regions of the brain, and more weakly expressed in the gut. In adults, highly expressed in the thymus and intestine.1 Publication

Gene expression databases

BgeeiENSMUSG00000049502.
GenevisibleiQ3UIR3. MM.

Interactioni

Subunit structurei

Homodimer and heterodimer. Can heterodimerize with DTX1, enhancing its ubiquitin ligase activity in vitro. Interacts with PARP9/BAL1. Found in a complex with MYO6 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ3UIR3. 4 interactions.
MINTiMINT-1858179.
STRINGi10090.ENSMUSP00000080601.

Structurei

3D structure databases

ProteinModelPortaliQ3UIR3.
SMRiQ3UIR3. Positions 565-611, 614-748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Deltex family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri569 – 60840RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEH5. Eukaryota.
ENOG4111QEI. LUCA.
GeneTreeiENSGT00440000035370.
HOGENOMiHOG000112292.
HOVERGENiHBG051417.
InParanoidiQ3UIR3.
KOiK06058.
OMAiDLNCNLF.
OrthoDBiEOG091G06M5.
PhylomeDBiQ3UIR3.
TreeFamiTF325526.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UIR3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSPDPPSP LLVRLRESIP KAHRKLEIYF QSRASGGGEC SVQPVGPSAP
60 70 80 90 100
DTYEVKFLKK ADKEKVLKKS EHEMLVHNKP VTIVLETTKK PVEDLRPRLP
110 120 130 140 150
SLTQPVETPS SRPPSLTGSL DEALCDDIHP QDGLVSNSVD SVVQKIFLAV
160 170 180 190 200
TAELNCDLLS KEQRASITTV CPHIIKSMEG SDGIKKVCGN FKDIEKIHHF
210 220 230 240 250
LSEQLLEREQ KRKGSEQKRK CAPQKHTPPD VEREPPDQSS IQVPVLLLEY
260 270 280 290 300
FKHVNPGRLE FIEYKFGVNI EIQASSPNMV TVGFTSSPFG NVEEASQSFV
310 320 330 340 350
RDFQKCSQSL KQDCISLEEH QRAKEVRQEL SRCFPKLLIK GQGRTLTLLG
360 370 380 390 400
SPADISAATE KVSQGLGLRP VKITASGYTT GIEVDSTRFK LLEPELLQEI
410 420 430 440 450
SEIEQKFNTR GKVQEKGQKT CILFVPKDKD LDLSVQSYTG FTDAFQRATW
460 470 480 490 500
QLRTEVLSLK GLGKERARLH NTKFADNFKK EHPNVHFVTS QESVTLTGLP
510 520 530 540 550
HHLAQAMQYV SKRMGLAPSS GEKLAMDQET PMEISSSDPH GDQQENAALP
560 570 580 590 600
APRGTSSSPA ASKGTEDYCV ICMDTISNKH VLPKCKHEFC TSCISKAMLI
610 620 630 640 650
KPVCPVCLTS YGIQKGNQPE GTMSYSTQKG SLPGYEGCGT IVINYEIKDG
660 670 680 690 700
IQTKEHPNPG KAYHGTRRTA YLPDNTEGRK VLDLLHEAFK HRLTFTIGYS
710 720 730 740
RATGVSDVIT WNDIHHKTSK FGGPANFGYP DPDYLKRVKE ELKAKGIE
Length:748
Mass (Da):83,044
Last modified:October 11, 2005 - v1
Checksum:iC9CD9704F66DCF1B
GO
Isoform 2 (identifier: Q3UIR3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     654-748: KEHPNPGKAY...KEELKAKGIE → VSVPEEHGFL...NDFPRKSFIL

Note: No experimental confirmation available.
Show »
Length:691
Mass (Da):76,579
Checksum:i782067CD356EA5F6
GO

Sequence cautioni

The sequence BAC41115 differs from that shown. Reason: Frameshift at position 746. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei654 – 74895KEHPN…AKGIE → VSVPEEHGFLFAMLELPKSN EASYSLLDNDFPRKSFIL in isoform 2. 1 PublicationVSP_038533Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146802 mRNA. Translation: BAE27443.1.
AK090152 mRNA. Translation: BAC41115.1. Frameshift.
CH466521 Genomic DNA. Translation: EDK97899.1.
BC137694 mRNA. Translation: AAI37695.1.
CCDSiCCDS37325.1. [Q3UIR3-1]
RefSeqiNP_001013389.2. NM_001013371.2. [Q3UIR3-1]
UniGeneiMm.390852.

Genome annotation databases

EnsembliENSMUST00000081933; ENSMUSP00000080601; ENSMUSG00000049502. [Q3UIR3-1]
ENSMUST00000114885; ENSMUSP00000110535; ENSMUSG00000049502. [Q3UIR3-2]
GeneIDi209200.
KEGGimmu:209200.
UCSCiuc007zbv.1. mouse. [Q3UIR3-1]
uc007zbw.1. mouse. [Q3UIR3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146802 mRNA. Translation: BAE27443.1.
AK090152 mRNA. Translation: BAC41115.1. Frameshift.
CH466521 Genomic DNA. Translation: EDK97899.1.
BC137694 mRNA. Translation: AAI37695.1.
CCDSiCCDS37325.1. [Q3UIR3-1]
RefSeqiNP_001013389.2. NM_001013371.2. [Q3UIR3-1]
UniGeneiMm.390852.

3D structure databases

ProteinModelPortaliQ3UIR3.
SMRiQ3UIR3. Positions 565-611, 614-748.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3UIR3. 4 interactions.
MINTiMINT-1858179.
STRINGi10090.ENSMUSP00000080601.

PTM databases

iPTMnetiQ3UIR3.
PhosphoSiteiQ3UIR3.

Proteomic databases

EPDiQ3UIR3.
MaxQBiQ3UIR3.
PaxDbiQ3UIR3.
PeptideAtlasiQ3UIR3.
PRIDEiQ3UIR3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081933; ENSMUSP00000080601; ENSMUSG00000049502. [Q3UIR3-1]
ENSMUST00000114885; ENSMUSP00000110535; ENSMUSG00000049502. [Q3UIR3-2]
GeneIDi209200.
KEGGimmu:209200.
UCSCiuc007zbv.1. mouse. [Q3UIR3-1]
uc007zbw.1. mouse. [Q3UIR3-2]

Organism-specific databases

CTDi151636.
MGIiMGI:2656973. Dtx3l.

Phylogenomic databases

eggNOGiENOG410IEH5. Eukaryota.
ENOG4111QEI. LUCA.
GeneTreeiENSGT00440000035370.
HOGENOMiHOG000112292.
HOVERGENiHBG051417.
InParanoidiQ3UIR3.
KOiK06058.
OMAiDLNCNLF.
OrthoDBiEOG091G06M5.
PhylomeDBiQ3UIR3.
TreeFamiTF325526.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ3UIR3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049502.
GenevisibleiQ3UIR3. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDTX3L_MOUSE
AccessioniPrimary (citable) accession number: Q3UIR3
Secondary accession number(s): Q8BN11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: October 11, 2005
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.