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Protein

Trinucleotide repeat-containing gene 6A protein

Gene

Tnrc6a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs) (PubMed:22187428). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins (PubMed:22187428). As a scaffolding protein, associates with argonaute proteins bound to partially complementary mRNAs, and can simultaneously recruit CCR4-NOT and PAN deadenylase complexes (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein-containing complex scaffold activity Source: MGI
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processRNA-mediated gene silencing, Translation regulation

Enzyme and pathway databases

ReactomeiR-MMU-426496 Post-transcriptional silencing by small RNAs
R-MMU-5578749 Transcriptional regulation by small RNAs

Names & Taxonomyi

Protein namesi
Recommended name:
Trinucleotide repeat-containing gene 6A protein
Gene namesi
Name:Tnrc6a
Synonyms:Kiaa1460
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2385292 Tnrc6a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Development up until embryonic stage E9.5 is normal. After this point, mortality rates increase rapidly with less than 5% survival at stage E12.5. The yolk sac endoderm is abnormally thin with fewer mitotic cells. In addition there are fewer blood islands in the yolk sac, associated with impaired hematopoiesis. Expression of many miRNA-regulated genes in the yolk sac is abnormal, and in particular there is marked derepression of genes involved in regulation of apoptosis and the cell cycle. miRNA biogenesis is not affected.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003739801 – 1896Trinucleotide repeat-containing gene 6A proteinAdd BLAST1896

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei724PhosphoserineBy similarity1
Modified residuei863PhosphoserineBy similarity1
Modified residuei976PhosphoserineBy similarity1
Modified residuei1197PhosphoserineBy similarity1
Modified residuei1255PhosphoserineBy similarity1
Modified residuei1406PhosphothreonineBy similarity1
Modified residuei1520PhosphoserineBy similarity1
Modified residuei1804PhosphoserineBy similarity1
Modified residuei1825PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3UHK8
MaxQBiQ3UHK8
PaxDbiQ3UHK8
PRIDEiQ3UHK8

PTM databases

iPTMnetiQ3UHK8
PhosphoSitePlusiQ3UHK8

Expressioni

Developmental stagei

Detected in the yolk sac endoderm at embryonic stage E9.5.1 Publication

Interactioni

Subunit structurei

Interacts with AGO2. Interacts with AGO1, AGO3 and AGO4. Interacts with CNOT1; the interaction is direct and mediates the association with the CCR4-NOT complex. Interacts with ZC3H12A.By similarity

GO - Molecular functioni

  • protein-containing complex scaffold activity Source: MGI

Protein-protein interaction databases

BioGridi231455, 1 interactor
DIPiDIP-60538N
IntActiQ3UHK8, 3 interactors
STRINGi10090.ENSMUSP00000091595

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5UMZX-ray1.90D/E1164-1172[»]
ProteinModelPortaliQ3UHK8
SMRiQ3UHK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1716 – 1788RRMAdd BLAST73

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 917Interaction with argonaute family proteinsBy similarityAdd BLAST917
Regioni239 – 488Sufficient for interaction with AGO1, AGO3 and AGO4By similarityAdd BLAST250
Regioni255 – 331Sufficient for interaction with AGO2By similarityAdd BLAST77
Regioni303 – 384Sufficient for interaction with AGO2By similarityAdd BLAST82
Regioni325 – 424Sufficient for interaction with AGO2By similarityAdd BLAST100
Regioni394 – 480Sufficient for interaction with AGO2By similarityAdd BLAST87
Regioni487 – 736Sufficient for interaction with AGO2By similarityAdd BLAST250
Regioni551 – 1279Sufficient for interaction with AGO1 and AGO4By similarityAdd BLAST729
Regioni1059 – 1129Sufficient for interaction with AGO2By similarityAdd BLAST71
Regioni1605 – 1896Sufficient for interaction with AGO2By similarityAdd BLAST292

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 54Lys-richAdd BLAST24
Compositional biasi93 – 113Gln-richAdd BLAST21
Compositional biasi334 – 337Poly-Ser4
Compositional biasi1127 – 1130Poly-Glu4
Compositional biasi1332 – 1415Gln-richAdd BLAST84
Compositional biasi1592 – 1597Poly-Ser6
Compositional biasi1890 – 1893Poly-Gly4

Sequence similaritiesi

Belongs to the GW182 family.Curated

Phylogenomic databases

eggNOGiENOG410IF5S Eukaryota
ENOG41110AX LUCA
GeneTreeiENSGT00410000025966
HOGENOMiHOG000049171
InParanoidiQ3UHK8
KOiK18412
OMAiNLNSVRQ
PhylomeDBiQ3UHK8

Family and domain databases

CDDicd12711 RRM_TNRC6A, 1 hit
Gene3Di3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR019486 Argonaute_hook_dom
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR032226 TNRC6_PABC-bd
IPR033501 TNRC6A
IPR034924 TNRC6A_RRM
PANTHERiPTHR13020:SF28 PTHR13020:SF28, 2 hits
PfamiView protein in Pfam
PF10427 Ago_hook, 1 hit
PF16608 TNRC6-PABC_bdg, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit

Sequencei

Sequence statusi: Complete.

Q3UHK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRELEAKATK DVERNLSRDL VQEEEQLMEE KKKKKDDKKK KEAAQKKATE
60 70 80 90 100
QKIKVPEQIK PSVSQPQPAN SDNGTSTATS TNNNAKRATA SNQQPPPPQQ
110 120 130 140 150
QQPQQEQQQQ QPQALPRYPR EVPPRFRHQE HKQLLKRGQH FPVIAANLGS
160 170 180 190 200
AVKVLNSQSE SSAVTNQQPQ NNGEVQNSKS QSDINHNTSG SHYENCQRGP
210 220 230 240 250
VSSTSDCSTS CKNAVNDLLE KEAWPSAPGS DPELAPECID ADSASNSESE
260 270 280 290 300
RNITVMASGN TGGEKDGLRN STGLGSQSKF VVGSSSNNVG HGSSTGPWGF
310 320 330 340 350
PHGALISTCQ VSVDAPESKP ESSNNRMNAW GTVSSSSNGG LNPSTLNSAS
360 370 380 390 400
NHGAWPVLEN NGLALKGPVG SGSSGINIQC STIGQMPNNQ NINSKVSGSS
410 420 430 440 450
THGTWGSLQE TCEPEVSGTQ KVSFSGQPQN ITTETTGPNN TTNFMTSSLP
460 470 480 490 500
NSGSVQNNEL PTSNPGAWRV STMNHPQIQA PSVMNGTSLS HLSNGESKTG
510 520 530 540 550
GSYGTTWGAY GSNYSGDKCA GPNGQANGDT VNATLMQPGI NGPMGTNFQV
560 570 580 590 600
NTNKGGGVWE PGTVNSQSSP WGSGNGANSG GSRRGWGSPA QNTGTGLSSV
610 620 630 640 650
EWNKLPSNQH SNDSANGNGK KLTNGWKSTE EDDQGSATSQ TNEQNSVWAK
660 670 680 690 700
AGGTVESDGS AESTGRLEEK VTGESQSRDR RKIDQHTLLQ SIVNRTDLDP
710 720 730 740 750
RVLSNSGWGQ TPIKQNTAWD TETSPRGERK TDNGTEAWGS SATQTFNSGA
760 770 780 790 800
CTDKTSPNSN DTSSVSGWGD PKPTLRWGDS KGSNCQGGWE DDSAATGMIK
810 820 830 840 850
SNQWGGCKED KSTWNDSQKS KQGWGDGQKS SQGWSISAGD NWGESSRSNH
860 870 880 890 900
WGEANKKSSS GGSDSDRSIS GWNELGKTSS FTWGNNINPN NSSGWDESSK
910 920 930 940 950
PNSSQGWGDP PKCNQSLGWG DSSKPVSSPD WNKQQDIVGS WGIPPATSKP
960 970 980 990 1000
PGTGWLGGPI PAPAKEEEPT GWEEPSPESI RRKMEIDDGT SAWGDPSKYN
1010 1020 1030 1040 1050
YKNVNMWNKN IPEASGRSDQ QAQMHRLLPA ASAVSSKETS SGSGWGEPWA
1060 1070 1080 1090 1100
EPSTPATTVD NGTSAWGKPI DSGPSWGEPI TAASNASTWG SSSVGPQSLS
1110 1120 1130 1140 1150
KSGPKSMQDG WCGDDMPLPG SRPTGWEEEE DVEIGMWNSN SSQELNSSLN
1160 1170 1180 1190 1200
WPPYTKKMSS KGLSGKKRRR ERGMMKGGNK QEDAWINPFV KQFSNISFSR
1210 1220 1230 1240 1250
DSPEENVQSN KMDLSGGMLQ DKRMEIDKHS LNIGDYNRTV GKGPGSRPQI
1260 1270 1280 1290 1300
SKESSMERNP YFDKNGNPNM FGVGNTAAQP RGMQQPPAQP LSSSQPNLRA
1310 1320 1330 1340 1350
QVPPPLLSPQ VPVSLLKYAP NNGGLNPLFG PQQVAMLNQL SQLNQLSQIS
1360 1370 1380 1390 1400
QLQRLLAQQQ RAQSQRSAPS ANRQQQDQQG RPLSVQQQMM QQSRQLDPSL
1410 1420 1430 1440 1450
LVKQQTPPSQ QPLHQPAMKS FLDNVMPHTT PELQKGPSPV NAFSNFPIGL
1460 1470 1480 1490 1500
NSNLNVNMDM NSIKEPQSRL RKWTTVDSMS VNTSLDQNSS KHGAISSGFR
1510 1520 1530 1540 1550
LEESPFVPYD FMNSSTSPAS PPGSIGDGWP RAKSPNGSSS VNWPPEFRPG
1560 1570 1580 1590 1600
EPWKGYPNID PETDPYVTPG SVINSLSINT VREVDHLRDR NSGSSSSLNT
1610 1620 1630 1640 1650
TLPSTSAWSS IRASNYNVPL SSTAQSTSAR NSDSKLTWSP GSVTNTSLAH
1660 1670 1680 1690 1700
ELWKVPLPPK NITAPSRPPP GLTGQKPPLS TWDNSPLRVG GGWGNSDARY
1710 1720 1730 1740 1750
TPGSSWGESS SGRITNWLVL KNLTPQIDGS TLRTLCMQHG PLITFHLNLP
1760 1770 1780 1790 1800
HGNALVRYSS KEEVVKAQKS LHMCVLGNTT ILAEFASEEE ISRFFAQSQS
1810 1820 1830 1840 1850
LTPSPGWQSL GSSQSRLGSL DCSHSFSSRT DVNHWNGAGL SGANCGDLHG
1860 1870 1880 1890
TSLWGTPHYS TSLWGPPSSD PRGISSPSPI NAFLSVDHLG GGGESM
Length:1,896
Mass (Da):203,150
Last modified:January 31, 2018 - v2
Checksum:i58858C69497903BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti587G → E in BAE27849 (PubMed:16141072).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147327 mRNA Translation: BAE27849.1
AC138364 Genomic DNA No translation available.
AC141887 Genomic DNA No translation available.
CCDSiCCDS57583.1
RefSeqiNP_659174.3, NM_144925.3
UniGeneiMm.102305

Genome annotation databases

EnsembliENSMUST00000094053; ENSMUSP00000091595; ENSMUSG00000052707
GeneIDi233833
KEGGimmu:233833

Similar proteinsi

Entry informationi

Entry nameiTNR6A_MOUSE
AccessioniPrimary (citable) accession number: Q3UHK8
Secondary accession number(s): F6ZMJ4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 31, 2018
Last modified: May 23, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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