Q3UHK8 (TNR6A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trinucleotide repeat-containing gene 6A protein

Gene names

Name:	Tnrc6a
Synonyms:	Kiaa1460

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1896 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins By similarity.
Subunit structure	Interacts with EIF2C1/AGO1, EIF2C2/AGO2, EIF2C3/AGO3 and EIF2C4/AGO4 By similarity.
Subcellular location	Cytoplasm › P-body By similarity. Note: Mammalian P-bodies are also known as GW bodies (GWBs) By similarity.
Sequence similarities	Belongs to the GW182 family. Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Biological process	RNA-mediated gene silencing Translation regulation
Cellular component	Cytoplasm
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to starvation Inferred from direct assay. Source: MGI negative regulation of translation involved in gene silencing by miRNA Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasmic mRNA processing body Inferred from electronic annotation. Source: UniProtKB-SubCell micro-ribonucleoprotein complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1896	1896	Trinucleotide repeat-containing gene 6A protein		PRO_0000373980
Regions
	Domain	1716 – 1788	73	RRM
	Region	239 – 488	250	Sufficient for interaction with EIF2C1, EIF2C3 and EIF2C4 By similarity
	Region	255 – 331	77	Sufficient for interaction with EIF2C2 By similarity
	Region	303 – 384	82	Sufficient for interaction with EIF2C2 By similarity
	Region	325 – 424	100	Sufficient for interaction with EIF2C2 By similarity
	Region	394 – 480	87	Sufficient for interaction with EIF2C2 By similarity
	Region	487 – 736	250	Sufficient for interaction with EIF2C2 By similarity
	Region	551 – 1279	729	Sufficient for interaction with EIF2C1 and EIF2C4 By similarity
	Region	1059 – 1129	71	Sufficient for interaction with EIF2C2 By similarity
	Region	1605 – 1896	292	Sufficient for interaction with EIF2C2 By similarity
	Compositional bias	31 – 54	24	Lys-rich
	Compositional bias	93 – 113	21	Gln-rich
	Compositional bias	334 – 337	4	Poly-Ser
	Compositional bias	1127 – 1130	4	Poly-Glu
	Compositional bias	1332 – 1415	84	Gln-rich
	Compositional bias	1592 – 1597	6	Poly-Ser
	Compositional bias	1890 – 1893	4	Poly-Gly
Amino acid modifications
	Modified residue	976	1	Phosphoserine Ref.2
	Modified residue	1406	1	Phosphothreonine By similarity
	Modified residue	1520	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3UHK8 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: D70578F1D6531608	FASTA	1,896	203,222
	10 20 30 40 50 60 MRELEAKATK DVERNLSRDL VQEEEQLMEE KKKKKDDKKK KEAAQKKATE QKIKVPEQIK 70 80 90 100 110 120 PSVSQPQPAN SDNGTSTATS TNNNAKRATA SNQQPPPPQQ QQPQQEQQQQ QPQALPRYPR 130 140 150 160 170 180 EVPPRFRHQE HKQLLKRGQH FPVIAANLGS AVKVLNSQSE SSAVTNQQPQ NNGEVQNSKS 190 200 210 220 230 240 QSDINHNTSG SHYENCQRGP VSSTSDCSTS CKNAVNDLLE KEAWPSAPGS DPELAPECID 250 260 270 280 290 300 ADSASNSESE RNITVMASGN TGGEKDGLRN STGLGSQSKF VVGSSSNNVG HGSSTGPWGF 310 320 330 340 350 360 PHGALISTCQ VSVDAPESKP ESSNNRMNAW GTVSSSSNGG LNPSTLNSAS NHGAWPVLEN 370 380 390 400 410 420 NGLALKGPVG SGSSGINIQC STIGQMPNNQ NINSKVSGSS THGTWGSLQE TCEPEVSGTQ 430 440 450 460 470 480 KVSFSGQPQN ITTETTGPNN TTNFMTSSLP NSGSVQNNEL PTSNPGAWRV STMNHPQIQA 490 500 510 520 530 540 PSVMNGTSLS HLSNGESKTG GSYGTTWGAY GSNYSGDKCA GPNGQANGDT VNATLMQPGI 550 560 570 580 590 600 NGPMGTNFQV NTNKGGGVWE PGTVNSQSSP WGSGNGANSG GSRRGWESPA QNTGTGLSSV 610 620 630 640 650 660 EWNKLPSNQH SNDSANGNGK KLTNGWKSTE EDDQGSATSQ TNEQNSVWAK AGGTVESDGS 670 680 690 700 710 720 AESTGRLEEK VTGESQSRDR RKIDQHTLLQ SIVNRTDLDP RVLSNSGWGQ TPIKQNTAWD 730 740 750 760 770 780 TETSPRGERK TDNGTEAWGS SATQTFNSGA CTDKTSPNSN DTSSVSGWGD PKPTLRWGDS 790 800 810 820 830 840 KGSNCQGGWE DDSAATGMIK SNQWGGCKED KSTWNDSQKS KQGWGDGQKS SQGWSISAGD 850 860 870 880 890 900 NWGESSRSNH WGEANKKSSS GGSDSDRSIS GWNELGKTSS FTWGNNINPN NSSGWDESSK 910 920 930 940 950 960 PNSSQGWGDP PKCNQSLGWG DSSKPVSSPD WNKQQDIVGS WGIPPATSKP PGTGWLGGPI 970 980 990 1000 1010 1020 PAPAKEEEPT GWEEPSPESI RRKMEIDDGT SAWGDPSKYN YKNVNMWNKN IPEASGRSDQ 1030 1040 1050 1060 1070 1080 QAQMHRLLPA ASAVSSKETS SGSGWGEPWA EPSTPATTVD NGTSAWGKPI DSGPSWGEPI 1090 1100 1110 1120 1130 1140 TAASNASTWG SSSVGPQSLS KSGPKSMQDG WCGDDMPLPG SRPTGWEEEE DVEIGMWNSN 1150 1160 1170 1180 1190 1200 SSQELNSSLN WPPYTKKMSS KGLSGKKRRR ERGMMKGGNK QEDAWINPFV KQFSNISFSR 1210 1220 1230 1240 1250 1260 DSPEENVQSN KMDLSGGMLQ DKRMEIDKHS LNIGDYNRTV GKGPGSRPQI SKESSMERNP 1270 1280 1290 1300 1310 1320 YFDKNGNPNM FGVGNTAAQP RGMQQPPAQP LSSSQPNLRA QVPPPLLSPQ VPVSLLKYAP 1330 1340 1350 1360 1370 1380 NNGGLNPLFG PQQVAMLNQL SQLNQLSQIS QLQRLLAQQQ RAQSQRSAPS ANRQQQDQQG 1390 1400 1410 1420 1430 1440 RPLSVQQQMM QQSRQLDPSL LVKQQTPPSQ QPLHQPAMKS FLDNVMPHTT PELQKGPSPV 1450 1460 1470 1480 1490 1500 NAFSNFPIGL NSNLNVNMDM NSIKEPQSRL RKWTTVDSMS VNTSLDQNSS KHGAISSGFR 1510 1520 1530 1540 1550 1560 LEESPFVPYD FMNSSTSPAS PPGSIGDGWP RAKSPNGSSS VNWPPEFRPG EPWKGYPNID 1570 1580 1590 1600 1610 1620 PETDPYVTPG SVINSLSINT VREVDHLRDR NSGSSSSLNT TLPSTSAWSS IRASNYNVPL 1630 1640 1650 1660 1670 1680 SSTAQSTSAR NSDSKLTWSP GSVTNTSLAH ELWKVPLPPK NITAPSRPPP GLTGQKPPLS 1690 1700 1710 1720 1730 1740 TWDNSPLRVG GGWGNSDARY TPGSSWGESS SGRITNWLVL KNLTPQIDGS TLRTLCMQHG 1750 1760 1770 1780 1790 1800 PLITFHLNLP HGNALVRYSS KEEVVKAQKS LHMCVLGNTT ILAEFASEEE ISRFFAQSQS 1810 1820 1830 1840 1850 1860 LTPSPGWQSL GSSQSRLGSL DCSHSFSSRT DVNHWNGAGL SGANCGDLHG TSLWGTPHYS 1870 1880 1890 TSLWGPPSSD PRGISSPSPI NAFLSVDHLG GGGESM

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-976, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK147327 mRNA. Translation: BAE27849.1.
IPI	IPI00670741.
RefSeq	NP_659174.3. NM_144925.3.
UniGene	Mm.102305.
3D structure databases
ProteinModelPortal	Q3UHK8.
SMR	Q3UHK8. Positions 1169-1246, 1709-1797.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3UHK8.
PTM databases
PhosphoSite	Q3UHK8.
Proteomic databases
PRIDE	Q3UHK8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	233833.
KEGG	mmu:233833.
UCSC	uc009jpb.1. mouse.
Organism-specific databases
CTD	27327.
MGI	MGI:2385292. Tnrc6a.
Rouge	Search...
Phylogenomic databases
HOGENOM	HBG446435.
InParanoid	Q3UHK8.
OrthoDB	EOG4DFPMT.
Gene expression databases
ArrayExpress	Q3UHK8.
Bgee	Q3UHK8.
Genevestigator	Q3UHK8.
Family and domain databases
InterPro	IPR019486. Argonaute_hook_dom. IPR012677. Nucleotide-bd_a/b_plait. IPR000504. RRM_dom. IPR009060. UBA-like. [Graphical view]
Gene3D	G3DSA:3.30.70.330. a_b_plait_nuc_bd. 1 hit.
Pfam	PF10427. Ago_hook. 1 hit. [Graphical view]
SMART	SM00360. RRM. 1 hit. [Graphical view]
SUPFAM	SSF46934. UBA_like. 1 hit.
PROSITE	PS50102. RRM. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	381871.
SOURCE	Search...

Entry information

Entry name

TNR6A_MOUSE

Accession

Primary (citable) accession number: Q3UHK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	October 11, 2005
Last modified:	November 16, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program