Reviewed, UniProtKB/Swiss-Prot Q3UHJ0 (AAK1_MOUSE)

Names and origin

Protein names

Recommended name:
    AP2-associated protein kinase 1
    EC=2.7.11.1
Alternative name(s):
    Adaptor-associated kinase 1

Gene names

Name:	Aak1
Synonyms:	Kiaa1048

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	959 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Stimulated by clathrin By similarity.
Subunit structure	Interacts with alpha-adaptin and AP-2 By similarity.
Subcellular location	Membrane; Peripheral membrane proteinBy similarity. Cell membrane; Peripheral membrane proteinBy similarity. Membrane › clathrin-coated pitBy similarity. Note= Active when found in clathrin-coated pits at the plasma membrane By similarity.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell membrane Coated pit Membrane
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3UHJ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3UHJ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     509-589: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 959	959	AP2-associated protein kinase 1		PRO_0000250579
Regions
	Domain	46 – 315	270	Protein kinase
	Nucleotide binding	52 – 60	9	ATP By similarity
	Compositional bias	22 – 25	4	Poly-Gly
	Compositional bias	397 – 612	216	Gln-rich
	Compositional bias	656 – 661	6	Poly-Ala
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	74	1	ATP By similarity
Amino acid modifications
	Modified residue	21	1	Phosphoserine
	Modified residue	221	1	Phosphothreonine By similarity
	Modified residue	222	1	Phosphothreonine By similarity
	Modified residue	234	1	Phosphotyrosine By similarity
	Modified residue	235	1	Phosphoserine By similarity
	Modified residue	389	1	Phosphothreonine By similarity
	Modified residue	604	1	Phosphothreonine
	Modified residue	616	1	Phosphoserine By similarity
	Modified residue	618	1	Phosphothreonine
	Modified residue	621	1	Phosphoserine
	Modified residue	622	1	Phosphoserine By similarity
	Modified residue	635	1	Phosphoserine
	Modified residue	638	1	Phosphothreonine
	Modified residue	650	1	Phosphoserine
	Modified residue	668	1	Phosphoserine By similarity
	Modified residue	674	1	Phosphoserine By similarity
	Modified residue	729	1	Phosphoserine
	Modified residue	935	1	Phosphoserine
	Modified residue	936	1	Phosphoserine
Natural variations
	Alternative sequence	509 – 589	81	Missing in isoform 2.		VSP_020670
Experimental info
	Sequence conflict	79	1	N → K in BAE27867. Ref.1
	Sequence conflict	686	1	N → S in BAC98082. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: B7D666EFD56D097A	FASTA	959	103,346
	10 20 30 40 50 60 MKKFFDSRRE QGSSGLGSGS SGGGGSSSGL GSGYIGRVFG IGRQQVTVDE VLAEGGFALV 70 80 90 100 110 120 FLVRTSNGVK CALKRMFVNN EHDLQVCKRE IQIMRDLSGH KNIVGYIDSS INNVSSGDVW 130 140 150 160 170 180 EVLILMDFCR GGQVVNLMNQ RLQTGFTENE VLQIFCDTCE AVARLHQCKT PIIHRDLKVE 190 200 210 220 230 240 NILLHDRGHY VLCDFGSATN KFQNPQAEGV NAVEDEIKKY TTLSYRAPEM VNLYSGKIIT 250 260 270 280 290 300 TKADIWALGC LLYKLCYFTL PFGESQVAIC DGSFTIPDNS RYSQDMHCLI RYMLEPDPDK 310 320 330 340 350 360 RPDIYQVSYF SFKLLKKECP VPNVQNSPIP AKLPEPVKAS EAAVKKTQPK ARLTDPIPTT 370 380 390 400 410 420 ETSIAPRQRP KAGQTQPNPG ILPIQPALTP RKRATVQPLP QAAGPSNQPG LLPSVSQPKA 430 440 450 460 470 480 QATPSQPLQS SQPKQPQAPP TPQQTPATQT QGLPTQAQAT PQHQQQHLLK QQQQQQQQPQ 490 500 510 520 530 540 QPTAPPQPAG TFYQQQQQQQ QQQAQTQQFQ AVHPAAQQPV TAQFPVGSQG GAQQQLMQNF 550 560 570 580 590 600 YHQQQQQQQQ QQQLMAQQAA LQQKTAVVVP QSQAQPATAP QAAAAQEPGQ IQAPVRQQPK 610 620 630 640 650 660 VQTTPPPTIQ GQKVGSLTPP SSPKTQRAGH RRILSDVTHS AVFGVPASKS TQLLQAAAAE 670 680 690 700 710 720 ASLNKSKSAT TTPSGSPRTS QQNVSNASEG STWNPFDDDN FSKLTAEELL NKDFAKLGEG 730 740 750 760 770 780 KLPEKLGGSA ESLIPGFQPT QGDAFTTPSF SAGTAEKRKG GQAVDSGIPL LSVSDPFIPL 790 800 810 820 830 840 QVPDAPEKLI EGLKSPDTSL LLPDLLPMTD PFGSTSDAVI DKADVAVESL IPGLEPPVAQ 850 860 870 880 890 900 RLPSQTESVT SNRTDSLTGE DSLLDCSLLS NPTAGLLEEF APIALSAPTH KAAEDSNLIS 910 920 930 940 950 GFGVAEGSEK VAEDEFDPIP VLITKNTQGG HSRNSSGSSE SSLPNLARSL LLVDQLIDL
	Isoform 2 [UniParc]. Checksum: 3AE736423984C27D		878	94,661
	10 20 30 40 50 60 MKKFFDSRRE QGSSGLGSGS SGGGGSSSGL GSGYIGRVFG IGRQQVTVDE VLAEGGFALV 70 80 90 100 110 120 FLVRTSNGVK CALKRMFVNN EHDLQVCKRE IQIMRDLSGH KNIVGYIDSS INNVSSGDVW 130 140 150 160 170 180 EVLILMDFCR GGQVVNLMNQ RLQTGFTENE VLQIFCDTCE AVARLHQCKT PIIHRDLKVE 190 200 210 220 230 240 NILLHDRGHY VLCDFGSATN KFQNPQAEGV NAVEDEIKKY TTLSYRAPEM VNLYSGKIIT 250 260 270 280 290 300 TKADIWALGC LLYKLCYFTL PFGESQVAIC DGSFTIPDNS RYSQDMHCLI RYMLEPDPDK 310 320 330 340 350 360 RPDIYQVSYF SFKLLKKECP VPNVQNSPIP AKLPEPVKAS EAAVKKTQPK ARLTDPIPTT 370 380 390 400 410 420 ETSIAPRQRP KAGQTQPNPG ILPIQPALTP RKRATVQPLP QAAGPSNQPG LLPSVSQPKA 430 440 450 460 470 480 QATPSQPLQS SQPKQPQAPP TPQQTPATQT QGLPTQAQAT PQHQQQHLLK QQQQQQQQPQ 490 500 510 520 530 540 QPTAPPQPAG TFYQQQQQQQ QQQAQTQQQI QAPVRQQPKV QTTPPPTIQG QKVGSLTPPS 550 560 570 580 590 600 SPKTQRAGHR RILSDVTHSA VFGVPASKST QLLQAAAAEA SLNKSKSATT TPSGSPRTSQ 610 620 630 640 650 660 QNVSNASEGS TWNPFDDDNF SKLTAEELLN KDFAKLGEGK LPEKLGGSAE SLIPGFQPTQ 670 680 690 700 710 720 GDAFTTPSFS AGTAEKRKGG QAVDSGIPLL SVSDPFIPLQ VPDAPEKLIE GLKSPDTSLL 730 740 750 760 770 780 LPDLLPMTDP FGSTSDAVID KADVAVESLI PGLEPPVAQR LPSQTESVTS NRTDSLTGED 790 800 810 820 830 840 SLLDCSLLSN PTAGLLEEFA PIALSAPTHK AAEDSNLISG FGVAEGSEKV AEDEFDPIPV 850 860 870 LITKNTQGGH SRNSSGSSES SLPNLARSLL LVDQLIDL

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Visual cortex.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H. DNA Res. 10:167-180(2003) [PubMed: 14621295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 417-959 (ISOFORM 1). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 473-959 (ISOFORM 2). Strain: C57BL/6. Tissue: Brain.
[4]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; THR-604; THR-618; SER-621; SER-635; SER-650; SER-729; SER-935 AND SER-936, MASS SPECTROMETRY. Tissue: Brain cortex.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-635; THR-638 AND SER-650, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK147363 mRNA. Translation: BAE27867.1. AK158879 mRNA. Translation: BAE34710.1. AK129272 mRNA. Translation: BAC98082.1. BC043125 mRNA. Translation: AAH43125.1.
RefSeq	NP_001035195.1. NP_808430.2.
UniGene	Mm.221038 Mm.466728
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q3UHJ0.
Genome annotation databases
Ensembl	ENSMUSG00000057230. Mus musculus. [Contig view]
GeneID	269774.
KEGG	mmu:269774.
Organism-specific databases
MGI	MGI:1098687. Aak1.
Rouge	Search...
Phylogenomic databases
HOVERGEN	Q3UHJ0.
Gene expression databases
ArrayExpress	Q3UHJ0.
GermOnline	ENSMUSG00000057230. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	393024.
SOURCE	Search...

Entry information

Entry name

AAK1_MOUSE

Accession

Primary (citable) accession number: Q3UHJ0
Secondary accession number(s): Q3TY53, Q6ZPZ6, Q80XP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 3, 2006
Last modified:	November 25, 2008
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)