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Q3UHD9 (AGAP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Short name=AGAP-2
Alternative name(s):
Centaurin-gamma-1
Short name=Cnt-g1
Phosphatidylinositol 3-kinase enhancer
Short name=PIKE
Gene names
Name:Agap2
Synonyms:Centg1, Kiaa0167
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1186 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. Aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase By similarity.

Enzyme regulation

GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation By similarity.

Subunit structure

Interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2 By similarity.

Subcellular location

Cytoplasm. Nucleus By similarity.

Domain

G domain binds GTP and has GTPase activity.

Arf-GAP domain interacts with G domain and may regulate its GTPase activity.

PH domain binds phospholipids and is required for nuclear targeting.

Sequence similarities

Belongs to the centaurin gamma-like family.

Contains 2 ANK repeats.

Contains 1 Arf-GAP domain.

Contains 1 PH domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainANK repeat
Repeat
Zinc-finger
   LigandGTP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionGTPase activation
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmammary gland alveolus development

Inferred from mutant phenotype PubMed 20075866. Source: MGI

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 16841086PubMed 20075866. Source: MGI

negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Inferred from electronic annotation. Source: Ensembl

positive regulation of JAK-STAT cascade

Inferred from mutant phenotype PubMed 20075866. Source: MGI

positive regulation of mammary gland epithelial cell proliferation

Inferred from mutant phenotype PubMed 20075866. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 20075866. Source: MGI

regulation of ARF GTPase activity

Inferred from electronic annotation. Source: InterPro

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: Ensembl

nucleolus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionARF GTPase activator activity

Inferred from electronic annotation. Source: InterPro

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 20075866. Source: MGI

protein kinase activator activity

Inferred from electronic annotation. Source: Ensembl

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3UHD9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3UHD9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     847-866: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11861186Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
PRO_0000235913

Regions

Domain670 – 904235PH
Domain925 – 1045121Arf-GAP
Repeat1084 – 111330ANK 1
Repeat1117 – 114630ANK 2
Nucleotide binding407 – 4148GTP Potential
Nucleotide binding451 – 4555GTP Potential
Nucleotide binding509 – 5124GTP Potential
Zinc finger940 – 96324C4-type
Region1 – 2323Interaction with EPB41L1 By similarity
Region180 – 22546Interactions with HOMER1 and NF2 By similarity
Region262 – 384123Interaction with PLCG1 By similarity
Region399 – 566168G domain

Amino acid modifications

Modified residue6321Phosphoserine By similarity

Natural variations

Alternative sequence847 – 86620Missing in isoform 2.
VSP_018542

Experimental info

Sequence conflict3811T → A in BAD90236. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: E9B4F3EDB66CBEC8

FASTA1,186124,511
        10         20         30         40         50         60 
MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAAG APGARGSEPR DPGSPRGSEE 

        70         80         90        100        110        120 
PGKKRHERLF HRQDALWIST SSAGTGGAEP PALSPAPASP ARPVSPAPGR RLSLWAAPPG 

       130        140        150        160        170        180 
PPLSGGLSPD SKPGGAPSSS RRPLLSSPSW GGPEPEGRTG GGVPGSSSPH PGTGSRRLKV 

       190        200        210        220        230        240 
APPPPAPKPF KTVTTSGAKA GGGKGAGSRL SWPESEGKPR VKGSKSTAGT GASAAAAGGG 

       250        260        270        280        290        300 
GSAAVTTSGG VGAGAGTRGK LSPRKGKSKT LDNSDLHPGP SAGSPPLTVP AIPVPATSVT 

       310        320        330        340        350        360 
ATSTQPLGPA PPITLEPPAP GLKRGREGGR ASTRDRKMLK FISGIFTKST GGPPGPGPLP 

       370        380        390        400        410        420 
GPQGLSSSSG SRELLGAELR TSPKAVVNSQ EWTLSRSIPE LRLGVLGDVR SGKSSLIHRF 

       430        440        450        460        470        480 
LTGSYQVLEK IESEQYKKEM LVDGQTHLVL IREEAGAPDA KFSGWADAVI FVFSLEDESS 

       490        500        510        520        530        540 
FQAVSRLHGQ LSSLRGEGRG GLALALVGTQ DRISASSPRV VGDARARALC TDMKRCSYYE 

       550        560        570        580        590        600 
TCATYGLNVD RVFQEVAQKV VTLRKQQQLL AACKSLPSSP SHSAASTPVA GQASNGGHTS 

       610        620        630        640        650        660 
DYSSSLPSSP NVGHRELRAE AAAVAGLSTP GSLHRAAKRR TSLFANRRGS DAEKRSLDSR 

       670        680        690        700        710        720 
GETTGSGRAI PIKQSFLLKR SGNSLNKEWK KKYVTLSSNG FLLYHPSIND YIHSTHGKEM 

       730        740        750        760        770        780 
DLLRTTVKVP GKRPPRAISA FGPSASINGL VKDMSTVQMG EGPEASTPMP SPSPSPSSLQ 

       790        800        810        820        830        840 
LPTDQTSKHL LKPDRNLARA LSTDCTPSGD LSPLSREPPP SPMVKKQRRK KLSTPSKTEG 

       850        860        870        880        890        900 
SAVQAEAKRK MWKLKSFGSL RNIYKAEENF EFLIVSSTGQ TWHFEAASFE ERDAWVQAIE 

       910        920        930        940        950        960 
SQILASLQCC ESSKVKLRTD SQSEAVAIQA IRNAKGNSTC VDCGAPNPTW ASLNLGALIC 

       970        980        990       1000       1010       1020 
IECSGIHRNL GTHLSRVRSL DLDDWPRELT LVLTAIGNDT ANRVWESDTR GRAKPTRDSS 

      1030       1040       1050       1060       1070       1080 
REERESWIRA KYEQLLFLAP LGTTEEPLGR QLWAAVEAQD VAAVLLLLAH ARHGPLDTSV 

      1090       1100       1110       1120       1130       1140 
EDPQLRSPLH LAAELAHVVI TQLLLWYGAD VAARDAQGRT ALFYARQAGS QLCADILLQH 

      1150       1160       1170       1180 
GCPGEGGSTA TTPSAATTPS ITATPSPRRR SSAASLGRVD TTIALV 

« Hide

Isoform 2 [UniParc].

Checksum: 6148D768484401DA
Show »

FASTA1,166122,103

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[2]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-1186 (ISOFORM 2).
Tissue: Fetal brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK147446 mRNA. Translation: BAE27918.1.
AK220325 mRNA. Translation: BAD90236.1.
CCDSCCDS24228.1. [Q3UHD9-1]
RefSeqNP_001028435.1. NM_001033263.4. [Q3UHD9-1]
XP_006513623.1. XM_006513560.1. [Q3UHD9-2]
UniGeneMm.336761.

3D structure databases

ProteinModelPortalQ3UHD9.
SMRQ3UHD9. Positions 396-570, 668-746, 867-908, 928-1150.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid229744. 3 interactions.
IntActQ3UHD9. 7 interactions.
MINTMINT-5046762.
STRING10090.ENSMUSP00000043466.

PTM databases

PhosphoSiteQ3UHD9.

Proteomic databases

PaxDbQ3UHD9.
PRIDEQ3UHD9.

Protocols and materials databases

DNASU216439.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000039259; ENSMUSP00000043466; ENSMUSG00000025422. [Q3UHD9-1]
GeneID216439.
KEGGmmu:216439.
UCSCuc007hhx.1. mouse. [Q3UHD9-1]
uc007hhy.1. mouse. [Q3UHD9-2]

Organism-specific databases

CTD116986.
MGIMGI:3580016. Agap2.
RougeSearch...

Phylogenomic databases

eggNOGCOG5347.
GeneTreeENSGT00740000115306.
HOGENOMHOG000007233.
HOVERGENHBG054045.
InParanoidQ3UHD9.
KOK17848.
OMADTANRVW.
OrthoDBEOG72VH5V.
PhylomeDBQ3UHD9.
TreeFamTF317762.

Gene expression databases

BgeeQ3UHD9.
GenevestigatorQ3UHD9.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR013684. MIRO-like.
IPR027417. P-loop_NTPase.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
[Graphical view]
PfamPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF08477. Miro. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSPR00405. REVINTRACTNG.
SMARTSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio375150.
PROQ3UHD9.
SOURCESearch...

Entry information

Entry nameAGAP2_MOUSE
AccessionPrimary (citable) accession number: Q3UHD9
Secondary accession number(s): Q5DU45
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot