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Q3UHD9

- AGAP2_MOUSE

UniProt

Q3UHD9 - AGAP2_MOUSE

Protein

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Gene

Agap2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. Aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase By similarity.By similarity

    Enzyme regulationi

    GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi407 – 4148GTPSequence Analysis
    Nucleotide bindingi451 – 4555GTPSequence Analysis
    Nucleotide bindingi509 – 5124GTPSequence Analysis
    Zinc fingeri940 – 96324C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ARF GTPase activator activity Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. protein kinase activator activity Source: Ensembl
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. mammary gland alveolus development Source: MGI
    2. negative regulation of apoptotic process Source: MGI
    3. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
    4. positive regulation of JAK-STAT cascade Source: MGI
    5. positive regulation of mammary gland epithelial cell proliferation Source: MGI
    6. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    7. regulation of ARF GTPase activity Source: InterPro
    8. small GTPase mediated signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Keywords - Ligandi

    GTP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
    Short name:
    AGAP-2
    Alternative name(s):
    Centaurin-gamma-1
    Short name:
    Cnt-g1
    Phosphatidylinositol 3-kinase enhancer
    Short name:
    PIKE
    Gene namesi
    Name:Agap2
    Synonyms:Centg1, Kiaa0167
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:3580016. Agap2.

    Subcellular locationi

    Cytoplasm. Nucleus By similarity

    GO - Cellular componenti

    1. mitochondrion Source: Ensembl
    2. nucleolus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11861186Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2PRO_0000235913Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei632 – 6321PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ3UHD9.
    PRIDEiQ3UHD9.

    PTM databases

    PhosphoSiteiQ3UHD9.

    Expressioni

    Gene expression databases

    BgeeiQ3UHD9.
    GenevestigatoriQ3UHD9.

    Interactioni

    Subunit structurei

    Interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2.By similarity

    Protein-protein interaction databases

    BioGridi229744. 3 interactions.
    IntActiQ3UHD9. 7 interactions.
    MINTiMINT-5046762.
    STRINGi10090.ENSMUSP00000043466.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3UHD9.
    SMRiQ3UHD9. Positions 396-570, 668-746, 867-908, 928-1150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini670 – 904235PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini925 – 1045121Arf-GAPPROSITE-ProRule annotationAdd
    BLAST
    Repeati1084 – 111330ANK 1Add
    BLAST
    Repeati1117 – 114630ANK 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 2323Interaction with EPB41L1By similarityAdd
    BLAST
    Regioni180 – 22546Interactions with HOMER1 and NF2By similarityAdd
    BLAST
    Regioni262 – 384123Interaction with PLCG1By similarityAdd
    BLAST
    Regioni399 – 566168G domainAdd
    BLAST

    Domaini

    G domain binds GTP and has GTPase activity.
    Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
    PH domain binds phospholipids and is required for nuclear targeting.

    Sequence similaritiesi

    Belongs to the centaurin gamma-like family.Curated
    Contains 2 ANK repeats.PROSITE-ProRule annotation
    Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri940 – 96324C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    ANK repeat, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5347.
    GeneTreeiENSGT00740000115306.
    HOGENOMiHOG000007233.
    HOVERGENiHBG054045.
    InParanoidiQ3UHD9.
    KOiK17848.
    OMAiDTANRVW.
    OrthoDBiEOG72VH5V.
    PhylomeDBiQ3UHD9.
    TreeFamiTF317762.

    Family and domain databases

    Gene3Di1.25.40.20. 1 hit.
    2.30.29.30. 2 hits.
    3.40.50.300. 1 hit.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001164. ArfGAP.
    IPR013684. MIRO-like.
    IPR027417. P-loop_NTPase.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view]
    PfamiPF12796. Ank_2. 1 hit.
    PF01412. ArfGap. 1 hit.
    PF08477. Miro. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view]
    PRINTSiPR00405. REVINTRACTNG.
    SMARTiSM00248. ANK. 2 hits.
    SM00105. ArfGap. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS50115. ARFGAP. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS51419. RAB. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3UHD9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAAG APGARGSEPR     50
    DPGSPRGSEE PGKKRHERLF HRQDALWIST SSAGTGGAEP PALSPAPASP 100
    ARPVSPAPGR RLSLWAAPPG PPLSGGLSPD SKPGGAPSSS RRPLLSSPSW 150
    GGPEPEGRTG GGVPGSSSPH PGTGSRRLKV APPPPAPKPF KTVTTSGAKA 200
    GGGKGAGSRL SWPESEGKPR VKGSKSTAGT GASAAAAGGG GSAAVTTSGG 250
    VGAGAGTRGK LSPRKGKSKT LDNSDLHPGP SAGSPPLTVP AIPVPATSVT 300
    ATSTQPLGPA PPITLEPPAP GLKRGREGGR ASTRDRKMLK FISGIFTKST 350
    GGPPGPGPLP GPQGLSSSSG SRELLGAELR TSPKAVVNSQ EWTLSRSIPE 400
    LRLGVLGDVR SGKSSLIHRF LTGSYQVLEK IESEQYKKEM LVDGQTHLVL 450
    IREEAGAPDA KFSGWADAVI FVFSLEDESS FQAVSRLHGQ LSSLRGEGRG 500
    GLALALVGTQ DRISASSPRV VGDARARALC TDMKRCSYYE TCATYGLNVD 550
    RVFQEVAQKV VTLRKQQQLL AACKSLPSSP SHSAASTPVA GQASNGGHTS 600
    DYSSSLPSSP NVGHRELRAE AAAVAGLSTP GSLHRAAKRR TSLFANRRGS 650
    DAEKRSLDSR GETTGSGRAI PIKQSFLLKR SGNSLNKEWK KKYVTLSSNG 700
    FLLYHPSIND YIHSTHGKEM DLLRTTVKVP GKRPPRAISA FGPSASINGL 750
    VKDMSTVQMG EGPEASTPMP SPSPSPSSLQ LPTDQTSKHL LKPDRNLARA 800
    LSTDCTPSGD LSPLSREPPP SPMVKKQRRK KLSTPSKTEG SAVQAEAKRK 850
    MWKLKSFGSL RNIYKAEENF EFLIVSSTGQ TWHFEAASFE ERDAWVQAIE 900
    SQILASLQCC ESSKVKLRTD SQSEAVAIQA IRNAKGNSTC VDCGAPNPTW 950
    ASLNLGALIC IECSGIHRNL GTHLSRVRSL DLDDWPRELT LVLTAIGNDT 1000
    ANRVWESDTR GRAKPTRDSS REERESWIRA KYEQLLFLAP LGTTEEPLGR 1050
    QLWAAVEAQD VAAVLLLLAH ARHGPLDTSV EDPQLRSPLH LAAELAHVVI 1100
    TQLLLWYGAD VAARDAQGRT ALFYARQAGS QLCADILLQH GCPGEGGSTA 1150
    TTPSAATTPS ITATPSPRRR SSAASLGRVD TTIALV 1186
    Length:1,186
    Mass (Da):124,511
    Last modified:October 11, 2005 - v1
    Checksum:iE9B4F3EDB66CBEC8
    GO
    Isoform 2 (identifier: Q3UHD9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         847-866: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,166
    Mass (Da):122,103
    Checksum:i6148D768484401DA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti381 – 3811T → A in BAD90236. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei847 – 86620Missing in isoform 2. 1 PublicationVSP_018542Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK147446 mRNA. Translation: BAE27918.1.
    AK220325 mRNA. Translation: BAD90236.1.
    CCDSiCCDS24228.1. [Q3UHD9-1]
    RefSeqiNP_001028435.1. NM_001033263.4. [Q3UHD9-1]
    XP_006513623.1. XM_006513560.1. [Q3UHD9-2]
    UniGeneiMm.336761.

    Genome annotation databases

    EnsembliENSMUST00000039259; ENSMUSP00000043466; ENSMUSG00000025422. [Q3UHD9-1]
    GeneIDi216439.
    KEGGimmu:216439.
    UCSCiuc007hhx.1. mouse. [Q3UHD9-1]
    uc007hhy.1. mouse. [Q3UHD9-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK147446 mRNA. Translation: BAE27918.1 .
    AK220325 mRNA. Translation: BAD90236.1 .
    CCDSi CCDS24228.1. [Q3UHD9-1 ]
    RefSeqi NP_001028435.1. NM_001033263.4. [Q3UHD9-1 ]
    XP_006513623.1. XM_006513560.1. [Q3UHD9-2 ]
    UniGenei Mm.336761.

    3D structure databases

    ProteinModelPortali Q3UHD9.
    SMRi Q3UHD9. Positions 396-570, 668-746, 867-908, 928-1150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229744. 3 interactions.
    IntActi Q3UHD9. 7 interactions.
    MINTi MINT-5046762.
    STRINGi 10090.ENSMUSP00000043466.

    PTM databases

    PhosphoSitei Q3UHD9.

    Proteomic databases

    PaxDbi Q3UHD9.
    PRIDEi Q3UHD9.

    Protocols and materials databases

    DNASUi 216439.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039259 ; ENSMUSP00000043466 ; ENSMUSG00000025422 . [Q3UHD9-1 ]
    GeneIDi 216439.
    KEGGi mmu:216439.
    UCSCi uc007hhx.1. mouse. [Q3UHD9-1 ]
    uc007hhy.1. mouse. [Q3UHD9-2 ]

    Organism-specific databases

    CTDi 116986.
    MGIi MGI:3580016. Agap2.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG5347.
    GeneTreei ENSGT00740000115306.
    HOGENOMi HOG000007233.
    HOVERGENi HBG054045.
    InParanoidi Q3UHD9.
    KOi K17848.
    OMAi DTANRVW.
    OrthoDBi EOG72VH5V.
    PhylomeDBi Q3UHD9.
    TreeFami TF317762.

    Miscellaneous databases

    NextBioi 375150.
    PROi Q3UHD9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3UHD9.
    Genevestigatori Q3UHD9.

    Family and domain databases

    Gene3Di 1.25.40.20. 1 hit.
    2.30.29.30. 2 hits.
    3.40.50.300. 1 hit.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR001164. ArfGAP.
    IPR013684. MIRO-like.
    IPR027417. P-loop_NTPase.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view ]
    Pfami PF12796. Ank_2. 1 hit.
    PF01412. ArfGap. 1 hit.
    PF08477. Miro. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view ]
    PRINTSi PR00405. REVINTRACTNG.
    SMARTi SM00248. ANK. 2 hits.
    SM00105. ArfGap. 1 hit.
    SM00233. PH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    PS50115. ARFGAP. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-1186 (ISOFORM 2).
      Tissue: Fetal brain.

    Entry informationi

    Entry nameiAGAP2_MOUSE
    AccessioniPrimary (citable) accession number: Q3UHD9
    Secondary accession number(s): Q5DU45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3