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Q3UHD9

- AGAP2_MOUSE

UniProt

Q3UHD9 - AGAP2_MOUSE

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Protein
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Gene
Agap2, Centg1, Kiaa0167
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. Aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase By similarity.

Enzyme regulationi

GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi407 – 4148GTP Reviewed prediction
Nucleotide bindingi451 – 4555GTP Reviewed prediction
Nucleotide bindingi509 – 5124GTP Reviewed prediction
Zinc fingeri940 – 96324C4-type
Add
BLAST

GO - Molecular functioni

  1. ARF GTPase activator activity Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. protein binding Source: MGI
  4. protein kinase activator activity Source: Ensembl
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. mammary gland alveolus development Source: MGI
  2. negative regulation of apoptotic process Source: MGI
  3. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
  4. positive regulation of JAK-STAT cascade Source: MGI
  5. positive regulation of mammary gland epithelial cell proliferation Source: MGI
  6. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  7. regulation of ARF GTPase activity Source: InterPro
  8. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Short name:
AGAP-2
Alternative name(s):
Centaurin-gamma-1
Short name:
Cnt-g1
Phosphatidylinositol 3-kinase enhancer
Short name:
PIKE
Gene namesi
Name:Agap2
Synonyms:Centg1, Kiaa0167
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:3580016. Agap2.

Subcellular locationi

Cytoplasm. Nucleus By similarity

GO - Cellular componenti

  1. mitochondrion Source: Ensembl
  2. nucleolus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11861186Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
PRO_0000235913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei632 – 6321Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3UHD9.
PRIDEiQ3UHD9.

PTM databases

PhosphoSiteiQ3UHD9.

Expressioni

Gene expression databases

BgeeiQ3UHD9.
GenevestigatoriQ3UHD9.

Interactioni

Subunit structurei

Interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2 By similarity.

Protein-protein interaction databases

BioGridi229744. 3 interactions.
IntActiQ3UHD9. 7 interactions.
MINTiMINT-5046762.
STRINGi10090.ENSMUSP00000043466.

Structurei

3D structure databases

ProteinModelPortaliQ3UHD9.
SMRiQ3UHD9. Positions 396-570, 668-746, 867-908, 928-1150.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini670 – 904235PH
Add
BLAST
Domaini925 – 1045121Arf-GAP
Add
BLAST
Repeati1084 – 111330ANK 1
Add
BLAST
Repeati1117 – 114630ANK 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2323Interaction with EPB41L1 By similarity
Add
BLAST
Regioni180 – 22546Interactions with HOMER1 and NF2 By similarity
Add
BLAST
Regioni262 – 384123Interaction with PLCG1 By similarity
Add
BLAST
Regioni399 – 566168G domain
Add
BLAST

Domaini

G domain binds GTP and has GTPase activity.
Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
PH domain binds phospholipids and is required for nuclear targeting.

Sequence similaritiesi

Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain.

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00740000115306.
HOGENOMiHOG000007233.
HOVERGENiHBG054045.
InParanoidiQ3UHD9.
KOiK17848.
OMAiDTANRVW.
OrthoDBiEOG72VH5V.
PhylomeDBiQ3UHD9.
TreeFamiTF317762.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR013684. MIRO-like.
IPR027417. P-loop_NTPase.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF08477. Miro. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3UHD9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAAG APGARGSEPR     50
DPGSPRGSEE PGKKRHERLF HRQDALWIST SSAGTGGAEP PALSPAPASP 100
ARPVSPAPGR RLSLWAAPPG PPLSGGLSPD SKPGGAPSSS RRPLLSSPSW 150
GGPEPEGRTG GGVPGSSSPH PGTGSRRLKV APPPPAPKPF KTVTTSGAKA 200
GGGKGAGSRL SWPESEGKPR VKGSKSTAGT GASAAAAGGG GSAAVTTSGG 250
VGAGAGTRGK LSPRKGKSKT LDNSDLHPGP SAGSPPLTVP AIPVPATSVT 300
ATSTQPLGPA PPITLEPPAP GLKRGREGGR ASTRDRKMLK FISGIFTKST 350
GGPPGPGPLP GPQGLSSSSG SRELLGAELR TSPKAVVNSQ EWTLSRSIPE 400
LRLGVLGDVR SGKSSLIHRF LTGSYQVLEK IESEQYKKEM LVDGQTHLVL 450
IREEAGAPDA KFSGWADAVI FVFSLEDESS FQAVSRLHGQ LSSLRGEGRG 500
GLALALVGTQ DRISASSPRV VGDARARALC TDMKRCSYYE TCATYGLNVD 550
RVFQEVAQKV VTLRKQQQLL AACKSLPSSP SHSAASTPVA GQASNGGHTS 600
DYSSSLPSSP NVGHRELRAE AAAVAGLSTP GSLHRAAKRR TSLFANRRGS 650
DAEKRSLDSR GETTGSGRAI PIKQSFLLKR SGNSLNKEWK KKYVTLSSNG 700
FLLYHPSIND YIHSTHGKEM DLLRTTVKVP GKRPPRAISA FGPSASINGL 750
VKDMSTVQMG EGPEASTPMP SPSPSPSSLQ LPTDQTSKHL LKPDRNLARA 800
LSTDCTPSGD LSPLSREPPP SPMVKKQRRK KLSTPSKTEG SAVQAEAKRK 850
MWKLKSFGSL RNIYKAEENF EFLIVSSTGQ TWHFEAASFE ERDAWVQAIE 900
SQILASLQCC ESSKVKLRTD SQSEAVAIQA IRNAKGNSTC VDCGAPNPTW 950
ASLNLGALIC IECSGIHRNL GTHLSRVRSL DLDDWPRELT LVLTAIGNDT 1000
ANRVWESDTR GRAKPTRDSS REERESWIRA KYEQLLFLAP LGTTEEPLGR 1050
QLWAAVEAQD VAAVLLLLAH ARHGPLDTSV EDPQLRSPLH LAAELAHVVI 1100
TQLLLWYGAD VAARDAQGRT ALFYARQAGS QLCADILLQH GCPGEGGSTA 1150
TTPSAATTPS ITATPSPRRR SSAASLGRVD TTIALV 1186
Length:1,186
Mass (Da):124,511
Last modified:October 11, 2005 - v1
Checksum:iE9B4F3EDB66CBEC8
GO
Isoform 2 (identifier: Q3UHD9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     847-866: Missing.

Note: No experimental confirmation available.

Show »
Length:1,166
Mass (Da):122,103
Checksum:i6148D768484401DA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei847 – 86620Missing in isoform 2.
VSP_018542Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti381 – 3811T → A in BAD90236. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK147446 mRNA. Translation: BAE27918.1.
AK220325 mRNA. Translation: BAD90236.1.
CCDSiCCDS24228.1. [Q3UHD9-1]
RefSeqiNP_001028435.1. NM_001033263.4. [Q3UHD9-1]
XP_006513623.1. XM_006513560.1. [Q3UHD9-2]
UniGeneiMm.336761.

Genome annotation databases

EnsembliENSMUST00000039259; ENSMUSP00000043466; ENSMUSG00000025422. [Q3UHD9-1]
GeneIDi216439.
KEGGimmu:216439.
UCSCiuc007hhx.1. mouse. [Q3UHD9-1]
uc007hhy.1. mouse. [Q3UHD9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK147446 mRNA. Translation: BAE27918.1 .
AK220325 mRNA. Translation: BAD90236.1 .
CCDSi CCDS24228.1. [Q3UHD9-1 ]
RefSeqi NP_001028435.1. NM_001033263.4. [Q3UHD9-1 ]
XP_006513623.1. XM_006513560.1. [Q3UHD9-2 ]
UniGenei Mm.336761.

3D structure databases

ProteinModelPortali Q3UHD9.
SMRi Q3UHD9. Positions 396-570, 668-746, 867-908, 928-1150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 229744. 3 interactions.
IntActi Q3UHD9. 7 interactions.
MINTi MINT-5046762.
STRINGi 10090.ENSMUSP00000043466.

PTM databases

PhosphoSitei Q3UHD9.

Proteomic databases

PaxDbi Q3UHD9.
PRIDEi Q3UHD9.

Protocols and materials databases

DNASUi 216439.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000039259 ; ENSMUSP00000043466 ; ENSMUSG00000025422 . [Q3UHD9-1 ]
GeneIDi 216439.
KEGGi mmu:216439.
UCSCi uc007hhx.1. mouse. [Q3UHD9-1 ]
uc007hhy.1. mouse. [Q3UHD9-2 ]

Organism-specific databases

CTDi 116986.
MGIi MGI:3580016. Agap2.
Rougei Search...

Phylogenomic databases

eggNOGi COG5347.
GeneTreei ENSGT00740000115306.
HOGENOMi HOG000007233.
HOVERGENi HBG054045.
InParanoidi Q3UHD9.
KOi K17848.
OMAi DTANRVW.
OrthoDBi EOG72VH5V.
PhylomeDBi Q3UHD9.
TreeFami TF317762.

Miscellaneous databases

NextBioi 375150.
PROi Q3UHD9.
SOURCEi Search...

Gene expression databases

Bgeei Q3UHD9.
Genevestigatori Q3UHD9.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR013684. MIRO-like.
IPR027417. P-loop_NTPase.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF08477. Miro. 1 hit.
PF00169. PH. 1 hit.
[Graphical view ]
PRINTSi PR00405. REVINTRACTNG.
SMARTi SM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-1186 (ISOFORM 2).
    Tissue: Fetal brain.

Entry informationi

Entry nameiAGAP2_MOUSE
AccessioniPrimary (citable) accession number: Q3UHD9
Secondary accession number(s): Q5DU45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 11, 2005
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi