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Q3UHD9

- AGAP2_MOUSE

UniProt

Q3UHD9 - AGAP2_MOUSE

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Protein

Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Gene

Agap2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. Aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase (By similarity).By similarity

Enzyme regulationi

GAP activity is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi407 – 4148GTPSequence Analysis
Nucleotide bindingi451 – 4555GTPSequence Analysis
Nucleotide bindingi509 – 5124GTPSequence Analysis
Zinc fingeri940 – 96324C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ARF GTPase activator activity Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. protein kinase activator activity Source: Ensembl
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. mammary gland alveolus development Source: MGI
  2. negative regulation of apoptotic process Source: MGI
  3. negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
  4. positive regulation of JAK-STAT cascade Source: MGI
  5. positive regulation of mammary gland epithelial cell proliferation Source: MGI
  6. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  7. regulation of ARF GTPase activity Source: InterPro
  8. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Ligandi

GTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_229826. Netrin-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2
Short name:
AGAP-2
Alternative name(s):
Centaurin-gamma-1
Short name:
Cnt-g1
Phosphatidylinositol 3-kinase enhancer
Short name:
PIKE
Gene namesi
Name:Agap2
Synonyms:Centg1, Kiaa0167
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:3580016. Agap2.

Subcellular locationi

Cytoplasm. Nucleus By similarity

GO - Cellular componenti

  1. mitochondrion Source: Ensembl
  2. nucleolus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11861186Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2PRO_0000235913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei632 – 6321PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UHD9.
PaxDbiQ3UHD9.
PRIDEiQ3UHD9.

PTM databases

PhosphoSiteiQ3UHD9.

Expressioni

Gene expression databases

BgeeiQ3UHD9.
GenevestigatoriQ3UHD9.

Interactioni

Subunit structurei

Interacts with EPB41L1, PLCG1, NF2, HOMER1 and HOMER2.By similarity

Protein-protein interaction databases

BioGridi229744. 3 interactions.
IntActiQ3UHD9. 7 interactions.
MINTiMINT-5046762.
STRINGi10090.ENSMUSP00000043466.

Structurei

3D structure databases

ProteinModelPortaliQ3UHD9.
SMRiQ3UHD9. Positions 396-570, 668-746, 867-908, 928-1150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini670 – 904235PHPROSITE-ProRule annotationAdd
BLAST
Domaini925 – 1045121Arf-GAPPROSITE-ProRule annotationAdd
BLAST
Repeati1084 – 111330ANK 1Add
BLAST
Repeati1117 – 114630ANK 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2323Interaction with EPB41L1By similarityAdd
BLAST
Regioni180 – 22546Interactions with HOMER1 and NF2By similarityAdd
BLAST
Regioni262 – 384123Interaction with PLCG1By similarityAdd
BLAST
Regioni399 – 566168G domainAdd
BLAST

Domaini

G domain binds GTP and has GTPase activity.
Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
PH domain binds phospholipids and is required for nuclear targeting.

Sequence similaritiesi

Belongs to the centaurin gamma-like family.Curated
Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri940 – 96324C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5347.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000007233.
HOVERGENiHBG054045.
InParanoidiQ3UHD9.
KOiK17848.
OMAiDTANRVW.
OrthoDBiEOG72VH5V.
PhylomeDBiQ3UHD9.
TreeFamiTF317762.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR013684. MIRO-like.
IPR027417. P-loop_NTPase.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF08477. Miro. 1 hit.
PF00169. PH. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3UHD9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAAG APGARGSEPR
60 70 80 90 100
DPGSPRGSEE PGKKRHERLF HRQDALWIST SSAGTGGAEP PALSPAPASP
110 120 130 140 150
ARPVSPAPGR RLSLWAAPPG PPLSGGLSPD SKPGGAPSSS RRPLLSSPSW
160 170 180 190 200
GGPEPEGRTG GGVPGSSSPH PGTGSRRLKV APPPPAPKPF KTVTTSGAKA
210 220 230 240 250
GGGKGAGSRL SWPESEGKPR VKGSKSTAGT GASAAAAGGG GSAAVTTSGG
260 270 280 290 300
VGAGAGTRGK LSPRKGKSKT LDNSDLHPGP SAGSPPLTVP AIPVPATSVT
310 320 330 340 350
ATSTQPLGPA PPITLEPPAP GLKRGREGGR ASTRDRKMLK FISGIFTKST
360 370 380 390 400
GGPPGPGPLP GPQGLSSSSG SRELLGAELR TSPKAVVNSQ EWTLSRSIPE
410 420 430 440 450
LRLGVLGDVR SGKSSLIHRF LTGSYQVLEK IESEQYKKEM LVDGQTHLVL
460 470 480 490 500
IREEAGAPDA KFSGWADAVI FVFSLEDESS FQAVSRLHGQ LSSLRGEGRG
510 520 530 540 550
GLALALVGTQ DRISASSPRV VGDARARALC TDMKRCSYYE TCATYGLNVD
560 570 580 590 600
RVFQEVAQKV VTLRKQQQLL AACKSLPSSP SHSAASTPVA GQASNGGHTS
610 620 630 640 650
DYSSSLPSSP NVGHRELRAE AAAVAGLSTP GSLHRAAKRR TSLFANRRGS
660 670 680 690 700
DAEKRSLDSR GETTGSGRAI PIKQSFLLKR SGNSLNKEWK KKYVTLSSNG
710 720 730 740 750
FLLYHPSIND YIHSTHGKEM DLLRTTVKVP GKRPPRAISA FGPSASINGL
760 770 780 790 800
VKDMSTVQMG EGPEASTPMP SPSPSPSSLQ LPTDQTSKHL LKPDRNLARA
810 820 830 840 850
LSTDCTPSGD LSPLSREPPP SPMVKKQRRK KLSTPSKTEG SAVQAEAKRK
860 870 880 890 900
MWKLKSFGSL RNIYKAEENF EFLIVSSTGQ TWHFEAASFE ERDAWVQAIE
910 920 930 940 950
SQILASLQCC ESSKVKLRTD SQSEAVAIQA IRNAKGNSTC VDCGAPNPTW
960 970 980 990 1000
ASLNLGALIC IECSGIHRNL GTHLSRVRSL DLDDWPRELT LVLTAIGNDT
1010 1020 1030 1040 1050
ANRVWESDTR GRAKPTRDSS REERESWIRA KYEQLLFLAP LGTTEEPLGR
1060 1070 1080 1090 1100
QLWAAVEAQD VAAVLLLLAH ARHGPLDTSV EDPQLRSPLH LAAELAHVVI
1110 1120 1130 1140 1150
TQLLLWYGAD VAARDAQGRT ALFYARQAGS QLCADILLQH GCPGEGGSTA
1160 1170 1180
TTPSAATTPS ITATPSPRRR SSAASLGRVD TTIALV
Length:1,186
Mass (Da):124,511
Last modified:October 11, 2005 - v1
Checksum:iE9B4F3EDB66CBEC8
GO
Isoform 2 (identifier: Q3UHD9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     847-866: Missing.

Note: No experimental confirmation available.

Show »
Length:1,166
Mass (Da):122,103
Checksum:i6148D768484401DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti381 – 3811T → A in BAD90236. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei847 – 86620Missing in isoform 2. 1 PublicationVSP_018542Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147446 mRNA. Translation: BAE27918.1.
AK220325 mRNA. Translation: BAD90236.1.
CCDSiCCDS24228.1. [Q3UHD9-1]
RefSeqiNP_001028435.1. NM_001033263.5. [Q3UHD9-1]
NP_001287943.1. NM_001301014.1. [Q3UHD9-2]
UniGeneiMm.336761.

Genome annotation databases

EnsembliENSMUST00000039259; ENSMUSP00000043466; ENSMUSG00000025422. [Q3UHD9-1]
GeneIDi216439.
KEGGimmu:216439.
UCSCiuc007hhx.1. mouse. [Q3UHD9-1]
uc007hhy.1. mouse. [Q3UHD9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147446 mRNA. Translation: BAE27918.1 .
AK220325 mRNA. Translation: BAD90236.1 .
CCDSi CCDS24228.1. [Q3UHD9-1 ]
RefSeqi NP_001028435.1. NM_001033263.5. [Q3UHD9-1 ]
NP_001287943.1. NM_001301014.1. [Q3UHD9-2 ]
UniGenei Mm.336761.

3D structure databases

ProteinModelPortali Q3UHD9.
SMRi Q3UHD9. Positions 396-570, 668-746, 867-908, 928-1150.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 229744. 3 interactions.
IntActi Q3UHD9. 7 interactions.
MINTi MINT-5046762.
STRINGi 10090.ENSMUSP00000043466.

PTM databases

PhosphoSitei Q3UHD9.

Proteomic databases

MaxQBi Q3UHD9.
PaxDbi Q3UHD9.
PRIDEi Q3UHD9.

Protocols and materials databases

DNASUi 216439.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000039259 ; ENSMUSP00000043466 ; ENSMUSG00000025422 . [Q3UHD9-1 ]
GeneIDi 216439.
KEGGi mmu:216439.
UCSCi uc007hhx.1. mouse. [Q3UHD9-1 ]
uc007hhy.1. mouse. [Q3UHD9-2 ]

Organism-specific databases

CTDi 116986.
MGIi MGI:3580016. Agap2.
Rougei Search...

Phylogenomic databases

eggNOGi COG5347.
GeneTreei ENSGT00760000118874.
HOGENOMi HOG000007233.
HOVERGENi HBG054045.
InParanoidi Q3UHD9.
KOi K17848.
OMAi DTANRVW.
OrthoDBi EOG72VH5V.
PhylomeDBi Q3UHD9.
TreeFami TF317762.

Enzyme and pathway databases

Reactomei REACT_229826. Netrin-1 signaling.

Miscellaneous databases

NextBioi 375150.
PROi Q3UHD9.
SOURCEi Search...

Gene expression databases

Bgeei Q3UHD9.
Genevestigatori Q3UHD9.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
2.30.29.30. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR013684. MIRO-like.
IPR027417. P-loop_NTPase.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
[Graphical view ]
Pfami PF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF08477. Miro. 1 hit.
PF00169. PH. 1 hit.
[Graphical view ]
PRINTSi PR00405. REVINTRACTNG.
SMARTi SM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 138-1186 (ISOFORM 2).
    Tissue: Fetal brain.

Entry informationi

Entry nameiAGAP2_MOUSE
AccessioniPrimary (citable) accession number: Q3UHD9
Secondary accession number(s): Q5DU45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 11, 2005
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3