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Protein

Sorting nexin-27

Gene

Snx27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.1 Publication

GO - Molecular functioni

GO - Biological processi

  • endosomal transport Source: MGI
  • endosome to lysosome transport Source: MGI
  • intracellular protein transport Source: UniProtKB
  • response to drug Source: Ensembl
  • retrograde transport, endosome to plasma membrane Source: UniProtKB
  • signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-27
Gene namesi
Name:Snx27
Synonyms:Kiaa0488
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1923992. Snx27.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB-SubCell
  • early endosome Source: MGI
  • early endosome membrane Source: UniProtKB-SubCell
  • endosome Source: MGI
  • immunological synapse Source: UniProtKB
  • nucleoplasm Source: MGI
  • retromer complex Source: Ensembl
  • WASH complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Growth retardation followed by lethality. Some mice die in the uterus during embryonic development. Newborn mice that survive fail to thrive and all die at different times within the first 3 weeks. The body weight of newborn is lower than wild-type mice, and the postnatal growth is severely retarded, with a clear retardation of body weight gain. The growth retardation is not only reflected in the body weight, but also in multiple organs, such as the spleen, kidney, liver, heart and intestine. Mice also show neuronal deficits in the hippocampus and cortex: despite a normal neuroanatomy, defects in synaptic function, learning and memory and a reduction in the amounts of ionotropic glutamate receptors (NMDA and AMPA receptors) are observed.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 539539Sorting nexin-27PRO_0000315357Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei60 – 601PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UHD6.
PaxDbiQ3UHD6.
PeptideAtlasiQ3UHD6.
PRIDEiQ3UHD6.

PTM databases

iPTMnetiQ3UHD6.
PhosphoSiteiQ3UHD6.

Expressioni

Tissue specificityi

Expressed in cells of hematopoietic origin.1 Publication

Gene expression databases

BgeeiENSMUSG00000028136.
CleanExiMM_SNX27.
ExpressionAtlasiQ3UHD6. baseline and differential.
GenevisibleiQ3UHD6. MM.

Interactioni

Subunit structurei

Core component of the SNX27-retromer, a multiprotein complex composed of SNX27, the WASH complex and the retromer complex. Interacts (via the FERM-like regions) with the WASH complex. Interacts with SNX1. Interacts with CYTIP. Interacts with DGKZ. Interacts with MCC (By similarity). Interacts (via PDZ domain) with a number of target transmembrane proteins (via PDZ-binding motif): ABCC4, ADRB2, ARHGEF7, GRIA1, GRIA2, GRIN1, GRIN2A GRIN2C, KCNJ6, KCNJ9 and SLC2A1/GLUT1.By similarity2 Publications

Protein-protein interaction databases

BioGridi218291. 1 interaction.
DIPiDIP-60337N.
IntActiQ3UHD6. 2 interactions.
MINTiMINT-1858525.
STRINGi10090.ENSMUSP00000102904.

Structurei

3D structure databases

ProteinModelPortaliQ3UHD6.
SMRiQ3UHD6. Positions 39-133, 160-263, 272-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 13494PDZPROSITE-ProRule annotationAdd
BLAST
Domaini159 – 267109PXPROSITE-ProRule annotationAdd
BLAST
Domaini271 – 36090Ras-associatingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni271 – 36090FERM-like region F1By similarityAdd
BLAST
Regioni371 – 41949FERM-like region F2By similarityAdd
BLAST
Regioni423 – 523101FERM-like region F3By similarityAdd
BLAST

Domaini

The PDZ domain mediates binding to a subset of proteins containing a PDZ-binding motif at the C-terminus: the specificity for PDZ-binding motif is provided by the 2 residues located upstream of the canonical PDZ-binding motif. The PDZ domain also mediates binding to the retromer complex via direct interaction with VPS26 (VPS26A or VPS26B).By similarity
The PX domain mediates binding to phosphatidylinositol 3-phosphate (PtdIns(3P)) and localization to early endosome membranes.By similarity

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IMRD. Eukaryota.
ENOG410XPAD. LUCA.
GeneTreeiENSGT00530000063147.
HOGENOMiHOG000016641.
HOVERGENiHBG059206.
InParanoidiQ3UHD6.
KOiK17936.
OMAiCEQRKMV.
OrthoDBiEOG091G147N.
PhylomeDBiQ3UHD6.
TreeFamiTF318398.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001478. PDZ.
IPR001683. Phox.
IPR000159. RA_dom.
IPR028667. SNX27.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR12431:SF17. PTHR12431:SF17. 1 hit.
PfamiPF00595. PDZ. 1 hit.
PF00787. PX. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
PS50195. PX. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UHD6-1) [UniParc]FASTAAdd to basket
Also known as: SNX27a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADEDGEGIH PSAPHRNGGG GGGSGLHCAG NGGGGGGGPR VVRIVKSESG
60 70 80 90 100
YGFNVRGQVS EGGQLRSING ELYAPLQHVS AVLPGGAADR AGVRKGDRIL
110 120 130 140 150
EVNGVNVEGA THKQVVDLIR AGEKELILTV LSVPPHEADN LDPSDDSLGQ
160 170 180 190 200
SFYDYTEKQA VPISVPTYKH VEQNGEKFVV YNVYMAGRQL CSKRYREFAI
210 220 230 240 250
LHQNLKREFA NFTFPRLPGK WPFSLSEQQL DARRRGLEEY LEKVCSIRVI
260 270 280 290 300
GESDIMQEFL SESDENYNGV SDVELRVALP DGTTVTVRVK KNSTTDQVYQ
310 320 330 340 350
AIAAKVGMDS TTVNYFALFE VINHSFVRKL APNEFPHKLY VQNYTSAVPG
360 370 380 390 400
TCLTIRKWLF TTEEEVLLND NDLAVTYFFH QAVDDVKKGY IKAEEKSYQL
410 420 430 440 450
QKLHEQRKMV MYLNMLRTCE GYNEIIFPHC ACDSRRKGHV ITAISITHFK
460 470 480 490 500
LHACTEEGQL ENQVIAFEWD EMQRWDTDEE GMAFCFEYAR GEKKPRWVKI
510 520 530
FTPYFNYMHE CFERVFCELK WRKENIFQMA RSQQRDVAT
Length:539
Mass (Da):60,989
Last modified:January 15, 2008 - v2
Checksum:i0EF5B84253A90B4D
GO
Isoform 2 (identifier: Q3UHD6-2) [UniParc]FASTAAdd to basket
Also known as: SNX27b

The sequence of this isoform differs from the canonical sequence as follows:
     525-539: NIFQMARSQQRDVAT → EY

Show »
Length:526
Mass (Da):59,534
Checksum:i4623CCCAC326F5AC
GO

Sequence cautioni

The sequence BAB30966 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB30966 differs from that shown. Reason: Erroneous termination at position 258. Translated as Glu.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei525 – 53915NIFQM…RDVAT → EY in isoform 2. 2 PublicationsVSP_030540Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017836 mRNA. Translation: BAB30966.1. Sequence problems.
AK147452 mRNA. Translation: BAE27921.1.
AK122296 mRNA. Translation: BAC65578.1.
BC053495 mRNA. Translation: AAH53495.1.
CCDSiCCDS38537.1. [Q3UHD6-1]
CCDS38538.1. [Q3UHD6-2]
RefSeqiNP_001075953.1. NM_001082484.2. [Q3UHD6-1]
NP_083997.1. NM_029721.2. [Q3UHD6-2]
UniGeneiMm.28160.

Genome annotation databases

EnsembliENSMUST00000029783; ENSMUSP00000029783; ENSMUSG00000028136. [Q3UHD6-2]
ENSMUST00000107283; ENSMUSP00000102904; ENSMUSG00000028136. [Q3UHD6-1]
GeneIDi76742.
KEGGimmu:76742.
UCSCiuc008qgr.2. mouse. [Q3UHD6-2]
uc008qgs.2. mouse. [Q3UHD6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017836 mRNA. Translation: BAB30966.1. Sequence problems.
AK147452 mRNA. Translation: BAE27921.1.
AK122296 mRNA. Translation: BAC65578.1.
BC053495 mRNA. Translation: AAH53495.1.
CCDSiCCDS38537.1. [Q3UHD6-1]
CCDS38538.1. [Q3UHD6-2]
RefSeqiNP_001075953.1. NM_001082484.2. [Q3UHD6-1]
NP_083997.1. NM_029721.2. [Q3UHD6-2]
UniGeneiMm.28160.

3D structure databases

ProteinModelPortaliQ3UHD6.
SMRiQ3UHD6. Positions 39-133, 160-263, 272-523.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218291. 1 interaction.
DIPiDIP-60337N.
IntActiQ3UHD6. 2 interactions.
MINTiMINT-1858525.
STRINGi10090.ENSMUSP00000102904.

PTM databases

iPTMnetiQ3UHD6.
PhosphoSiteiQ3UHD6.

Proteomic databases

MaxQBiQ3UHD6.
PaxDbiQ3UHD6.
PeptideAtlasiQ3UHD6.
PRIDEiQ3UHD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029783; ENSMUSP00000029783; ENSMUSG00000028136. [Q3UHD6-2]
ENSMUST00000107283; ENSMUSP00000102904; ENSMUSG00000028136. [Q3UHD6-1]
GeneIDi76742.
KEGGimmu:76742.
UCSCiuc008qgr.2. mouse. [Q3UHD6-2]
uc008qgs.2. mouse. [Q3UHD6-1]

Organism-specific databases

CTDi81609.
MGIiMGI:1923992. Snx27.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IMRD. Eukaryota.
ENOG410XPAD. LUCA.
GeneTreeiENSGT00530000063147.
HOGENOMiHOG000016641.
HOVERGENiHBG059206.
InParanoidiQ3UHD6.
KOiK17936.
OMAiCEQRKMV.
OrthoDBiEOG091G147N.
PhylomeDBiQ3UHD6.
TreeFamiTF318398.

Miscellaneous databases

ChiTaRSiSnx27. mouse.
PROiQ3UHD6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028136.
CleanExiMM_SNX27.
ExpressionAtlasiQ3UHD6. baseline and differential.
GenevisibleiQ3UHD6. MM.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.30.1520.10. 1 hit.
InterProiIPR001478. PDZ.
IPR001683. Phox.
IPR000159. RA_dom.
IPR028667. SNX27.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR12431:SF17. PTHR12431:SF17. 1 hit.
PfamiPF00595. PDZ. 1 hit.
PF00787. PX. 1 hit.
PF00788. RA. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF54236. SSF54236. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
PS50195. PX. 1 hit.
PS50200. RA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNX27_MOUSE
AccessioniPrimary (citable) accession number: Q3UHD6
Secondary accession number(s): Q7TQL6, Q80TZ1, Q9CYB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: September 7, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.