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Protein

Adhesion G protein-coupled receptor B1

Gene

Adgrb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatidylserine receptor which enhances the engulfment of apoptotic cells (PubMed:17960134). Also mediates the binding and engulfment of Gram-negative bacteria (PubMed:21245295, PubMed:26838550, PubMed:26838550). Stimulates production of reactive oxygen species by macrophages in response to Gram-negative bacteria, resulting in enhanced microbicidal macrophage activity (By similarity). In the gastric mucosa, required for recognition and engulfment of apoptotic gastric epithelial cells (By similarity). Promotes myoblast fusion (PubMed:23615608). Activates the Rho pathway in a G-protein-dependent manner (By similarity). Inhibits MDM2-mediated ubiquitination and degradation of DLG4/PSD95, promoting DLG4 stability and regulating synaptic plasticity (PubMed:25751059). Required for the formation of dendritic spines by ensuring the correct localization of PARD3 and TIAM1 (By similarity). Potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53/TP53 signal in suppression of glioblastoma (By similarity).By similarity6 Publications
Vasculostatin-120: Inhibits angiogenesis in a CD36-dependent manner.By similarity
Vasculostatin-40: Inhibits angiogenesis.By similarity

GO - Molecular functioni

  • G-protein coupled receptor activity Source: UniProtKB
  • lipopolysaccharide binding Source: UniProtKB
  • PDZ domain binding Source: UniProtKB
  • phosphatidylserine binding Source: UniProtKB
  • receptor activity Source: UniProtKB

GO - Biological processi

  • apoptotic cell clearance Source: MGI
  • cell surface receptor signaling pathway Source: InterPro
  • defense response to Gram-negative bacterium Source: UniProtKB
  • engulfment of apoptotic cell Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • muscle organ development Source: UniProtKB-KW
  • negative regulation of angiogenesis Source: UniProtKB
  • negative regulation of endothelial cell migration Source: UniProtKB
  • negative regulation of protein catabolic process Source: UniProtKB
  • negative regulation of protein ubiquitination Source: UniProtKB
  • nervous system development Source: UniProtKB-KW
  • phagocytosis, engulfment Source: UniProtKB
  • phagocytosis, recognition Source: UniProtKB
  • positive regulation of myoblast fusion Source: UniProtKB
  • positive regulation of reactive oxygen species biosynthetic process Source: UniProtKB
  • positive regulation of synapse assembly Source: MGI
  • regulation of synaptic plasticity Source: UniProtKB

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processImmunity, Innate immunity, Myogenesis, Neurogenesis, Phagocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor B1Imported
Alternative name(s):
Brain-specific angiogenesis inhibitor 11 Publication
Cleaved into the following 2 chains:
Vasculostatin-120By similarity
Short name:
Vstat120By similarity
Vasculostatin-40By similarity
Short name:
Vstat-40By similarity
Gene namesi
Name:Adgrb1Imported
Synonyms:Bai11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1933736. Adgrb1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 948ExtracellularSequence analysisAdd BLAST915
Transmembranei949 – 969Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini970 – 980CytoplasmicSequence analysisAdd BLAST11
Transmembranei981 – 1001Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini1002 – 1008ExtracellularSequence analysis7
Transmembranei1009 – 1029Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini1030 – 1052CytoplasmicSequence analysisAdd BLAST23
Transmembranei1053 – 1073Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini1074 – 1093ExtracellularSequence analysisAdd BLAST20
Transmembranei1094 – 1114Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini1115 – 1136CytoplasmicSequence analysisAdd BLAST22
Transmembranei1137 – 1157Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini1158 – 1166ExtracellularSequence analysis9
Transmembranei1167 – 1187Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini1188 – 1582CytoplasmicSequence analysisAdd BLAST395

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Secreted, Synapse

Pathology & Biotechi

Disruption phenotypei

Viable and fertile with normal brain anatomy but mutants display severe deficits in hippocampus-dependent spatial learning and memory that are accompanied by enhanced long-term potentiation, impaired long-term depression, a thinning of the postsynaptic density at hippocampal synapses, reduced protein levels of Dlg4 and increased Dlg4 polyubiquitination (PubMed:25751059). Smaller myofibers than wild-type animals and impaired muscle regeneration after injury (PubMed:23615608). Impaired bacterial clearance following E.coli infection (PubMed:26838550). RNAi-mediated knockdown in embryos results in greatly reduced dendritic spine density and small but significant increases in spine length and decreases in spine diameter (PubMed:23595754).4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1487 – 1489RKR → AAA: Reduced binding to ELMO1 and failure to promote engulfment of apoptotic thymocytes. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000024504634 – 1582Adhesion G protein-coupled receptor B1Add BLAST1549
ChainiPRO_000044180734 – 926Vasculostatin-120By similarityAdd BLAST893
ChainiPRO_000044180634 – 327Vasculostatin-40By similarityAdd BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi273 ↔ 309PROSITE-ProRule annotation
Disulfide bondi277 ↔ 314PROSITE-ProRule annotation
Disulfide bondi288 ↔ 299PROSITE-ProRule annotation
Disulfide bondi366 ↔ 400PROSITE-ProRule annotation
Disulfide bondi370 ↔ 406PROSITE-ProRule annotation
Disulfide bondi381 ↔ 390PROSITE-ProRule annotation
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi421 ↔ 456PROSITE-ProRule annotation
Disulfide bondi425 ↔ 461PROSITE-ProRule annotation
Disulfide bondi436 ↔ 446PROSITE-ProRule annotation
Disulfide bondi479 ↔ 514PROSITE-ProRule annotation
Disulfide bondi483 ↔ 519PROSITE-ProRule annotation
Disulfide bondi494 ↔ 504PROSITE-ProRule annotation
Disulfide bondi534 ↔ 569PROSITE-ProRule annotation
Disulfide bondi538 ↔ 574PROSITE-ProRule annotation
Disulfide bondi549 ↔ 559PROSITE-ProRule annotation
Disulfide bondi581 ↔ 616PROSITE-ProRule annotation
Disulfide bondi604 ↔ 634PROSITE-ProRule annotation
Glycosylationi607N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei609PhosphothreonineBy similarity1
Glycosylationi692N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi844N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi877N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi881N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi884 ↔ 921PROSITE-ProRule annotation
Disulfide bondi909 ↔ 923PROSITE-ProRule annotation
Modified residuei1467PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved to produce vasculostatin-40 and vasculostatin-120. Vasculostatin-40 is the major form and is produced through proteolytic cleavage by MMP14 between residues 321 and 329 with cleavage likely to be between Ser-326 and Leu-327.By similarity
Ubiquitinated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei926 – 927CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ3UHD1.
PeptideAtlasiQ3UHD1.
PRIDEiQ3UHD1.

PTM databases

iPTMnetiQ3UHD1.
PhosphoSitePlusiQ3UHD1.

Expressioni

Tissue specificityi

In brain, widespread expression in all neuropil-rich zones including spinal cord gray matter, cerebellar molecular layer, cerebral cortex, thalamic nuclei and basal ganglia with no expression in white matter (at protein level) (PubMed:20888903). In the cerebellar molecular layer, highly expressed in interneuron processes whereas Purkinje cells and their dendrites show weaker expression (at protein level) (PubMed:20888903). In the olfactory bulb, highly expressed in glomeruli (at protein level) (PubMed:20888903). In the retina, highly concentrated in the outer and inner plexiform layers (at protein level) (PubMed:20888903). Expressed in brain (PubMed:11245925). Enriched in hippocampus and cortex (PubMed:23595754). Also detected in other tissues including bone marrow and spleen (PubMed:17960134).4 Publications

Gene expression databases

BgeeiENSMUSG00000034730.
CleanExiMM_BAI1.
ExpressionAtlasiQ3UHD1. baseline and differential.
GenevisibleiQ3UHD1. MM.

Interactioni

Subunit structurei

Interacts with ELMO1 and DOCK1 (PubMed:17960134). When bound to ELMO1 and DOCK1, acts as a module to promote apoptotic cell engulfment (PubMed:17960134). Interacts with MDM2; the interaction results in inhibition of MDM2-mediated ubiquitination and degradation of DLG4/PSD95 (PubMed:25751059). Interacts with PARD3 and TIAM1; the interaction is required for correct dendritic localization of PARD3 and TIAM1 and for dendritic spine formation (By similarity). Interacts with MAGI1, MAGI3 and BAIAP2 (By similarity). Interacts with PHYHIP (PubMed:11245925). Interacts with DLG4 (via PDZ domain) (By similarity). Vasculostatin-120: Interacts with CD36 (By similarity). Vasculostatin-120: Interacts with ARRB2 (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ELMO1Q92556-19EBI-911280,EBI-15668002From a different organism.

GO - Molecular functioni

  • PDZ domain binding Source: UniProtKB

Protein-protein interaction databases

BioGridi223612. 3 interactors.
DIPiDIP-37711N.
IntActiQ3UHD1. 4 interactors.
STRINGi10090.ENSMUSP00000046097.

Structurei

3D structure databases

ProteinModelPortaliQ3UHD1.
SMRiQ3UHD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini261 – 315TSP type-1 1PROSITE-ProRule annotationAdd BLAST55
Domaini354 – 407TSP type-1 2PROSITE-ProRule annotationAdd BLAST54
Domaini409 – 462TSP type-1 3PROSITE-ProRule annotationAdd BLAST54
Domaini467 – 520TSP type-1 4PROSITE-ProRule annotationAdd BLAST54
Domaini522 – 575TSP type-1 5PROSITE-ProRule annotationAdd BLAST54
Domaini881 – 938GPSPROSITE-ProRule annotationAdd BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni927 – 943N-terminal stalk following vasculostatin-120 cleavage which is not required for signaling activityBy similarityAdd BLAST17
Regioni1363 – 1582Involved in interaction with MAGI1By similarityAdd BLAST220
Regioni1579 – 1582Indispensable for interaction with MAGI1By similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1382 – 1449Pro-richAdd BLAST68

Domaini

The TSP type-1 repeats in the extracellular domain mediate binding to phosphatidylserine (PubMed:17960134). They are also required for bacterial recognition and binding to bacterial outer membrane lipopolysaccharide (PubMed:21245295).2 Publications

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEA0. Eukaryota.
ENOG4111FBM. LUCA.
GeneTreeiENSGT00900000140832.
HOGENOMiHOG000230916.
HOVERGENiHBG004813.
InParanoidiQ3UHD1.
KOiK04596.
OMAiSWRGCRT.
OrthoDBiEOG091G00C7.
PhylomeDBiQ3UHD1.
TreeFamiTF331634.

Family and domain databases

Gene3Di2.20.100.10. 5 hits.
InterProiView protein in InterPro
IPR032471. GAIN_dom_N.
IPR017981. GPCR_2-like.
IPR008077. GPCR_2_brain_angio_inhib.
IPR001879. GPCR_2_extracellular_dom.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
IPR000884. TSP1_rpt.
IPR036383. TSP1_rpt_sf.
PfamiView protein in Pfam
PF00002. 7tm_2. 1 hit.
PF16489. GAIN. 1 hit.
PF01825. GPS. 1 hit.
PF02793. HRM. 1 hit.
PF00090. TSP_1. 5 hits.
PRINTSiPR01694. BAIPRECURSOR.
PR00249. GPCRSECRETIN.
SMARTiView protein in SMART
SM00303. GPS. 1 hit.
SM00008. HormR. 1 hit.
SM00209. TSP1. 5 hits.
SUPFAMiSSF82895. SSF82895. 5 hits.
PROSITEiView protein in PROSITE
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50092. TSP1. 5 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UHD1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGQAAAPGP IWILAPLLLL LLLLGRWARA ASGADIGPGT EQCTTLVQGK
60 70 80 90 100
FFGYFSAAAV FPANASRCSW TLRNPDPRRY TLYMKVAKAP APCSGPGRVR
110 120 130 140 150
TYQFDSFLES TRTYLGVESF DEVLRLCDSS APLAFLQASK QFLQMQRQQP
160 170 180 190 200
PQDGDLGPQG EFPSSSDDFS VEYLVVGNRN PSHAACQMLC RWLDACLAGS
210 220 230 240 250
RSSHPCGIMQ TPCACLGGDV GDPASSPLVP RGDVCLRDGV AGGPENCLTS
260 270 280 290 300
LTQDRGGHGS AGGWKLWSLW GECTRDCGGG LQTRTRTCLP TLGVEGGGCE
310 320 330 340 350
GVLEEGRLCN RKACGPTGRS SSRSQSLRST DARRREEFGD ELQQFGFPSP
360 370 380 390 400
QTGDPAAEEW SPWSVCSSTC GEGWQTRTRF CVSSSYSTQC SGPLREQRLC
410 420 430 440 450
NNSAVCPVHG AWDEWSPWSL CSSTCGRGFR DRTRTCRPPQ FGGNPCEGPE
460 470 480 490 500
KQTKFCNIAL CPGRAVDGNW NEWSSWSTCS ASCSQGRQQR TRECNGPSYG
510 520 530 540 550
GAECQGHWVE TRDCFLQQCP VDGKWQAWAS WGSCSVTCGG GSQRRERVCS
560 570 580 590 600
GPFFGGAACQ GPQDEYRQCG AQRCPEPHEI CDEDNFGAVV WKETPAGEVA
610 620 630 640 650
AVRCPRNATG LILRRCELDE EGIAFWEPPT YIRCVSIDYR NIQMMTREHL
660 670 680 690 700
AKAQRGLPGE GVSEVIQTLL EISQDGTSYS GDLLSTIDVL RNMTEIFRRA
710 720 730 740 750
YYSPTPGDVQ NFVQIISNLL AEENRDKWEE AQLMGPNAKE LFRLVEDFVD
760 770 780 790 800
VIGFRMKDLR DAYQVTDNLV LSIHKLPASG ATDISFPMKG WRATGDWAKV
810 820 830 840 850
PEDRVTVSKS VFSTGLAEAD DSSVFVVGTV LYRNLGSFLA LQRNTTVLNS
860 870 880 890 900
KVISVTVKPP PRSLLTPLEI EFAHMYNGTT NQTCILWDET DGPSSSAPPQ
910 920 930 940 950
LGPWSWRGCR TVPLDALRTR CLCDRLSTFA ILAQLSADAT MDKVTVPSVT
960 970 980 990 1000
LIVGCGVSSL TLLMLVIIYV SVWRYIRSER SVILINFCLS IISSNALILI
1010 1020 1030 1040 1050
GQTQTRNKVV CTLVAAFLHF FFLSSFCWVL TEAWQSYMAV TGRLRSRLVR
1060 1070 1080 1090 1100
KRFLCLGWGL PALVVAISVG FTKAKGYSTM NYCWLSLEGG LLYAFVGPAA
1110 1120 1130 1140 1150
AVVLVNMVIG ILVFNKLVSK DGITDKKLKE RAGASLWSSC VVLPLLALTW
1160 1170 1180 1190 1200
MSAVLAVTDR RSALFQILFA VFDSLEGFVI VMVHCILRRE VQDAVKCRVV
1210 1220 1230 1240 1250
DRQEEGNGDS GGSFQNGHAQ LMTDFEKDVD LACRSVLNKD IAACRTATIT
1260 1270 1280 1290 1300
GTFKRPSLPE EEKMKLAKGP PPTFNSLPAN VSKLHLHGSP RYPGGPLPDF
1310 1320 1330 1340 1350
PNHSLTLKKD KAPKSSFIGD GDIFKKLDSE LSRAQEKALD TSYVILPTAT
1360 1370 1380 1390 1400
ATLRPKPKEE PKYSINIDQM PQTRLIHLSM APDASFPTRS PPAREPPGGA
1410 1420 1430 1440 1450
PPEVPPVQPP PPPPPPPPPP QQPIPPPPTL EPAPPSLGDT GEPAAHPGPS
1460 1470 1480 1490 1500
SGAGAKNENV ATLSVSSLER RKSRYAELDF EKIMHTRKRH QDMFQDLNRK
1510 1520 1530 1540 1550
LQHAAEKEKE VPGADSKPEK QQTPNKRAWE SLRKPHGTPA WVKKELEPLP
1560 1570 1580
PSPLELRSVE WEKAGATIPL VGQDIIDLQT EV
Length:1,582
Mass (Da):173,297
Last modified:October 11, 2005 - v1
Checksum:i6D1AA6D46E2E223B
GO
Isoform 2 (identifier: Q3UHD1-2)
Sequence is not available
Note: Observed very weakly in the kidney, skeletal muscle, skin, stomach, thymus and brain from embryonic day 18. By neonatal day 1, the expression is targeted only to the brain.
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti264W → L in AAN86966 (PubMed:11245925).Curated1
Sequence conflicti275 – 286RDCGG…QTRTR → AGLRGRPANSNP in AAN86966 (PubMed:11245925).CuratedAdd BLAST12
Sequence conflicti300E → K in AAN86966 (PubMed:11245925).Curated1
Sequence conflicti821D → G in BAE28021 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY168408 mRNA. Translation: AAN86966.1.
AK147494 mRNA. Translation: BAE27949.1.
AK147456 mRNA. Translation: BAE27923.1.
AK147459 mRNA. Translation: BAE27926.1.
AK147607 mRNA. Translation: BAE28021.1.
CCDSiCCDS70635.1. [Q3UHD1-1]
RefSeqiNP_778156.2. NM_174991.3. [Q3UHD1-1]
UniGeneiMm.43133.

Genome annotation databases

EnsembliENSMUST00000042035; ENSMUSP00000046097; ENSMUSG00000034730. [Q3UHD1-1]
GeneIDi107831.
KEGGimmu:107831.
UCSCiuc007wco.1. mouse. [Q3UHD1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAGRB1_MOUSE
AccessioniPrimary (citable) accession number: Q3UHD1
Secondary accession number(s): Q3UH36, Q8CGM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 11, 2005
Last modified: November 22, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families