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Protein

Trinucleotide repeat-containing gene 6C protein

Gene

Tnrc6c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in RNA-mediated gene silencing by micro-RNAs (miRNAs). Required for miRNA-dependent translational repression of complementary mRNAs by argonaute family proteins As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • embryonic hemopoiesis Source: MGI
  • embryonic organ development Source: MGI
  • endoderm development Source: MGI
  • endoderm formation Source: MGI
  • gene silencing by miRNA Source: MGI
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
  • positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
  • regulation of gene silencing by miRNA Source: MGI
Complete GO annotation...

Keywords - Biological processi

RNA-mediated gene silencing, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Trinucleotide repeat-containing gene 6C protein
Gene namesi
Name:Tnrc6c
Synonyms:Kiaa1582
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2443265. Tnrc6c.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16901690Trinucleotide repeat-containing gene 6C proteinPRO_0000278527Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei465 – 4651PhosphoserineCombined sources
Modified residuei714 – 7141PhosphoserineBy similarity
Modified residuei776 – 7761PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3UHC0.
MaxQBiQ3UHC0.
PaxDbiQ3UHC0.
PRIDEiQ3UHC0.

PTM databases

iPTMnetiQ3UHC0.
PhosphoSiteiQ3UHC0.

Expressioni

Gene expression databases

BgeeiQ3UHC0.
CleanExiMM_TNRC6C.

Interactioni

Subunit structurei

Interacts with one or more of the argonaute family proteins AGO1, AGO2, AGO3 and AGO4. Interacts with CNOT1; the interaction mediates the association with the CCR4-NOT complex. Interacts with PAN3; the interaction mediates the association with the PAN complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi229897. 1 interaction.
IntActiQ3UHC0. 1 interaction.
STRINGi10090.ENSMUSP00000026658.

Structurei

3D structure databases

ProteinModelPortaliQ3UHC0.
SMRiQ3UHC0. Positions 923-993, 1513-1593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini928 – 97346UBAPROSITE-ProRule annotationAdd
BLAST
Domaini1565 – 163268RRMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 921921Sufficient for interaction with argonaute family proteinsBy similarityAdd
BLAST
Regioni1260 – 1690431Silencing domain; interaction with CNOT1 and PAN3By similarityAdd
BLAST
Regioni1371 – 1690320Sufficient for translational repression when tethered to a target mRNABy similarityAdd
BLAST
Regioni1371 – 141747Required for interaction with PABPC1By similarityAdd
BLAST
Regioni1381 – 139919PABPC1-interacting motif-2 (PAM2)By similarityAdd
BLAST
Regioni1596 – 169095Interaction with the CCR4-NOT complexBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1156 – 121459Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi57 – 604Poly-Ser
Compositional biasi204 – 430227Gly-richAdd
BLAST
Compositional biasi904 – 9107Poly-Ser
Compositional biasi1215 – 124834Pro-richAdd
BLAST

Domaini

The silencing domain, also known as C-terminal effector domain (CED), can act in autonomous repression, including both translational inhibition and mRNA degradation.By similarity

Sequence similaritiesi

Belongs to the GW182 family.Curated
Contains 1 UBA domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFAU. Eukaryota.
ENOG41110AX. LUCA.
HOVERGENiHBG062594.
InParanoidiQ3UHC0.
KOiK18412.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR019486. Argonaute_hook_dom.
IPR026805. GW182_M_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR032226. TNRC6_PABC-bd.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF10427. Ago_hook. 1 hit.
PF12938. M_domain. 1 hit.
PF00076. RRM_1. 1 hit.
PF16608. TNRC6-PABC_bdg. 2 hits.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UHC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATGSAQSSF PSHLKKTNGS HGTNGALVQS PSNQSALGAG GTNGNGGVAR
60 70 80 90 100
VWGVATSSSS GLAHCSVGGG DGKMDNMIGD GRSQNCWGAS NSNAGINLNL
110 120 130 140 150
NPNANPAAWP VLGHEGTVAT GNPSSICSPV SAIGQNMGSQ NGNPVGALGA
160 170 180 190 200
WGNLLPQESA EPQTSTSQNV SFSVQPQNLN TDGPNNTNPM NSSPNPINAM
210 220 230 240 250
QTNGLPNWGM AVGMGAIIPP HLQGLPGANG SSVSQGSGSG GEGMGSSVWG
260 270 280 290 300
LSPGNPATGS TNCGFSQGNG DTVNSALSAK QNGSSSAVQK EGNGGNAWDS
310 320 330 340 350
GPPAGPGILA WGRGSGTNGI GNIHSGAWGH PSRSSSNGVN GEWGKPPNQH
360 370 380 390 400
SSSDISGKGS TGWDSASAAS QTPALQPGSE HMNSWAKATS SGTTASEGSS
410 420 430 440 450
DGSGNHNEGS TGREGTGEGR RRDKGVLDQG HIQLPRNDLD PRVLSNSGWG
460 470 480 490 500
QTPVKQNTAW EFEESPRSER KNDNGTEAWG SIATQPSNSG GKTDGSIMNS
510 520 530 540 550
TNTSSVSGWV SSPPAAVPAN TSWGDSNNKA PSGPGVWGDS ISSTAVNNAA
560 570 580 590 600
ATKSGHAWSG TVNQEDKSPT WGEPQKPKSQ NWGDGQRANP AWSTGAGDWA
610 620 630 640 650
DSSSVLGHLG DGKKNGSGWD ADGNRSGSGW NDATRCGTSG WGSGTNAKVN
660 670 680 690 700
PGTNWGESLK PGPQQNWAHK PQDNNVSNWG GAASVKQTGT GWIGGPLPVK
710 720 730 740 750
QKDSSEATGW EEPSPPSIRR KMEIDDGTSA WGDPSTYNNK TVNMWDRNNP
760 770 780 790 800
VIQSSTTAPA TPTTPTSSST THRAETPPSH QAGTQLNRSP LLGPVSSGWG
810 820 830 840 850
EMPSVHSKAE NSWGEPSSPC TLVDNGTAAW GKPPSSGSGW DHPAEPVVPF
860 870 880 890 900
GRASAPAAAP ALCKPASKSM QEGWGSGADE MNLGTSQWED EDGDMWNNAA
910 920 930 940 950
SQESSSSCSS WGNTSKKGLQ KGMKTPGKQD EAWIMSRLIK QLTDMGFPRE
960 970 980 990 1000
PAEEALKSNS MNLDQAMSAL LEKKVDMDKR GLGMTDYNGM VTKPLGCRPP
1010 1020 1030 1040 1050
ISKESSMDRP TFLDKLTLSF SNQDGGLVEE PTTSPFLPSP SLKLPLSNSA
1060 1070 1080 1090 1100
LPSQALGGVA SGLGMQNLNS SRQIPSGNLG VFGNSGAAQA RTMQQPPVQP
1110 1120 1130 1140 1150
LNSSQPSLRA QVPQFLSPQV QAQLLQFAAK NIGLSPALLT SPINPQHMTM
1160 1170 1180 1190 1200
LNQLYQLQLA YQRLQIQQQM LQAQRNVSGP MRQQEQQVAR TITNLQQQIQ
1210 1220 1230 1240 1250
QHQRQLAQAL LVKQPPPPPP PPHLSLHPSA GKSGMESFPP PPQAPGLPDL
1260 1270 1280 1290 1300
QTKEQQSSPN TFAPYPLAGL NPNMNVNSID MSSGLSVKDP SQSQSRLPQW
1310 1320 1330 1340 1350
THPNSMGNLS SAASPLDQNP SKHGAIPGGL SIGPPGKSSI DDSYGRYDLI
1360 1370 1380 1390 1400
QNSESPASPP VAVPHSWSRA KSDSDKISNG SSISWPPEFH PGVPWKGLQN
1410 1420 1430 1440 1450
IDPENDPDVT PGSVPTGPTI NTTIQDVNRY LLKSGGKLSD IKSTWSSGPA
1460 1470 1480 1490 1500
SHTQASLSHE LWKVPRNTTA PTRPPPGLAN PKPSSTWGTS PLGWTSSYSS
1510 1520 1530 1540 1550
GSAWSTDTSG RTSSWLVLRN LTPQIDGSTL RTLCLQHGPL ITFHLNLTQG
1560 1570 1580 1590 1600
NAVVRYSSKE EAAKAQKSLH MCVLGNTTIL AEFAGEEEVN RFLAQGQALP
1610 1620 1630 1640 1650
PTSSWQSSSG GSQPRLGTSG STHGLVRSDT AHWNTPCLSG KGSSELLWGG
1660 1670 1680 1690
VPQYSSSLWG PPSAEDARVI GSPTPLNTLL PGDLLSGESI
Length:1,690
Mass (Da):175,775
Last modified:February 20, 2007 - v2
Checksum:i4788764371891904
GO

Sequence cautioni

The sequence BAE27937.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1521 – 15211L → P in BAE21997 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047249 mRNA. Translation: BAC33005.1.
AK134064 mRNA. Translation: BAE21997.1.
AK147471 mRNA. Translation: BAE27937.1. Different initiation.
AK122529 mRNA. Translation: BAC65811.1.
RefSeqiNP_932139.2. NM_198022.2.
UniGeneiMm.40272.
Mm.473244.

Genome annotation databases

GeneIDi217351.
KEGGimmu:217351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047249 mRNA. Translation: BAC33005.1.
AK134064 mRNA. Translation: BAE21997.1.
AK147471 mRNA. Translation: BAE27937.1. Different initiation.
AK122529 mRNA. Translation: BAC65811.1.
RefSeqiNP_932139.2. NM_198022.2.
UniGeneiMm.40272.
Mm.473244.

3D structure databases

ProteinModelPortaliQ3UHC0.
SMRiQ3UHC0. Positions 923-993, 1513-1593.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229897. 1 interaction.
IntActiQ3UHC0. 1 interaction.
STRINGi10090.ENSMUSP00000026658.

PTM databases

iPTMnetiQ3UHC0.
PhosphoSiteiQ3UHC0.

Proteomic databases

EPDiQ3UHC0.
MaxQBiQ3UHC0.
PaxDbiQ3UHC0.
PRIDEiQ3UHC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi217351.
KEGGimmu:217351.

Organism-specific databases

CTDi57690.
MGIiMGI:2443265. Tnrc6c.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IFAU. Eukaryota.
ENOG41110AX. LUCA.
HOVERGENiHBG062594.
InParanoidiQ3UHC0.
KOiK18412.

Miscellaneous databases

ChiTaRSiTnrc6c. mouse.
PROiQ3UHC0.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UHC0.
CleanExiMM_TNRC6C.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR019486. Argonaute_hook_dom.
IPR026805. GW182_M_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR032226. TNRC6_PABC-bd.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF10427. Ago_hook. 1 hit.
PF12938. M_domain. 1 hit.
PF00076. RRM_1. 1 hit.
PF16608. TNRC6-PABC_bdg. 2 hits.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF54928. SSF54928. 1 hit.
PROSITEiPS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain, Cerebellum and Thymus.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 329-1690.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Spleen.

Entry informationi

Entry nameiTNR6C_MOUSE
AccessioniPrimary (citable) accession number: Q3UHC0
Secondary accession number(s): Q3UZ61, Q80TB6, Q8BXF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: June 8, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.