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Protein

N-acetylaspartate synthetase

Gene

Nat8l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake.4 Publications

Miscellaneous

Seems to modulate behavioral effects induced by methamphetamine in vivo.
'Shati' is the name of the symbol at Nagoya castle in Japan.

Catalytic activityi

Acetyl-CoA + L-aspartate = CoA + N-acetyl-L-aspartate.1 Publication

Enzyme regulationi

Aminooxyacetic acid (AOAA) blocks its activity in both cytoplasm and mitochondria.By similarity

GO - Molecular functioni

  • aspartate N-acetyltransferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.17. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylaspartate synthetase (EC:2.3.1.17)
Short name:
NAA synthetase
Alternative name(s):
N-acetyltransferase 8-like protein
Protein Shati
Gene namesi
Name:Nat8l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2447776. Nat8l.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei118 – 138HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003052301 – 299N-acetylaspartate synthetaseAdd BLAST299

Proteomic databases

MaxQBiQ3UGX3.
PaxDbiQ3UGX3.
PeptideAtlasiQ3UGX3.
PRIDEiQ3UGX3.

Expressioni

Tissue specificityi

Expressed in brain, kidney, liver and spleen. In brain, present in neurons but not in astrocytes (at protein level). Expressed in brain, thymus and spleen.3 Publications

Inductioni

By methamphetamine in brain, via dopamine receptor activation (at protein level).1 Publication

Gene expression databases

CleanExiMM_NAT8L.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059313.

Structurei

3D structure databases

ProteinModelPortaliQ3UGX3.
SMRiQ3UGX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 280N-acetyltransferasePROSITE-ProRule annotationAdd BLAST138

Sequence similaritiesi

Belongs to the camello family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IS8M. Eukaryota.
ENOG410YY26. LUCA.
HOGENOMiHOG000113735.
HOVERGENiHBG108173.
InParanoidiQ3UGX3.
KOiK18309.
PhylomeDBiQ3UGX3.
TreeFamiTF324687.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
PfamiView protein in Pfam
PF00583. Acetyltransf_1. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS51186. GNAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3UGX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCGPPDMVC ETKIVATEDH EALPGAKKDA LLVAAGAMWP PLPAAPGPAA
60 70 80 90 100
APPPAAGPQP HGGTGGAGPP EGRGVCIREF RAAEQEAARR IFYDGILERI
110 120 130 140 150
PNTAFRGLRQ HPRTQLLYAL LAALCFAVTR SLLLTCLVPA GLLALRYYYS
160 170 180 190 200
RKVILAYLEC ALHTDMADIE QYYMKPPGSC FWVAVLDGNV VGIVAARAHE
210 220 230 240 250
EDNTVELLRM SVDSRFRGKS IAKALGRRVL EFAMLHNYSA VVLGTTAVKV
260 270 280 290
AAHKLYESLG FRHMGASDHY VLPGMTLSLA ERLFFQVRYH RYRLQLREE
Length:299
Mass (Da):32,777
Last modified:October 11, 2005 - v1
Checksum:i488DC1B586265DAD
GO

Sequence cautioni

The sequence AAH75616 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence ABA54615 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti220S → G in BAE28014 (PubMed:16141072).Curated1
Sequence conflicti220S → G in ABA54615 (PubMed:17626222).Curated1
Sequence conflicti220S → G in AAH75616 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147307 mRNA. Translation: BAE27836.1.
AK147594 mRNA. Translation: BAE28014.1.
AK147703 mRNA. Translation: BAE28084.1.
DQ174094 mRNA. Translation: ABA54615.1. Different initiation.
BC034068 mRNA. Translation: AAH34068.1.
BC075616 mRNA. Translation: AAH75616.1. Different initiation.
CCDSiCCDS19210.1.
RefSeqiNP_001001985.3. NM_001001985.3.
UniGeneiMm.274610.

Genome annotation databases

GeneIDi269642.
KEGGimmu:269642.
UCSCiuc008xbs.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiNAT8L_MOUSE
AccessioniPrimary (citable) accession number: Q3UGX3
Secondary accession number(s): Q3UH43, Q6DID6, Q8K065
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 11, 2005
Last modified: August 30, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families