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Protein

N-acetylaspartate synthetase

Gene

Nat8l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake.4 Publications

Catalytic activityi

Acetyl-CoA + L-aspartate = CoA + N-acetyl-L-aspartate.1 Publication

Enzyme regulationi

Aminooxyacetic acid (AOAA) blocks its activity in both cytoplasm and mitochondria.By similarity

GO - Molecular functioni

  • aspartate N-acetyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.17. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylaspartate synthetase (EC:2.3.1.17)
Short name:
NAA synthetase
Alternative name(s):
N-acetyltransferase 8-like protein
Protein Shati
Gene namesi
Name:Nat8l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2447776. Nat8l.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299N-acetylaspartate synthetasePRO_0000305230Add
BLAST

Proteomic databases

MaxQBiQ3UGX3.
PaxDbiQ3UGX3.
PRIDEiQ3UGX3.

Expressioni

Tissue specificityi

Expressed in brain, kidney, liver and spleen. In brain, present in neurons but not in astrocytes (at protein level). Expressed in brain, thymus and spleen.3 Publications

Inductioni

By methamphetamine in brain, via dopamine receptor activation (at protein level).1 Publication

Gene expression databases

BgeeiQ3UGX3.
CleanExiMM_NAT8L.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059313.

Structurei

3D structure databases

ProteinModelPortaliQ3UGX3.
SMRiQ3UGX3. Positions 158-273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 280138N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the camello family.Curated
Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IS8M. Eukaryota.
ENOG410YY26. LUCA.
HOGENOMiHOG000113735.
HOVERGENiHBG108173.
InParanoidiQ3UGX3.
KOiK18309.
OrthoDBiEOG73Z2W2.
PhylomeDBiQ3UGX3.
TreeFamiTF324687.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UGX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCGPPDMVC ETKIVATEDH EALPGAKKDA LLVAAGAMWP PLPAAPGPAA
60 70 80 90 100
APPPAAGPQP HGGTGGAGPP EGRGVCIREF RAAEQEAARR IFYDGILERI
110 120 130 140 150
PNTAFRGLRQ HPRTQLLYAL LAALCFAVTR SLLLTCLVPA GLLALRYYYS
160 170 180 190 200
RKVILAYLEC ALHTDMADIE QYYMKPPGSC FWVAVLDGNV VGIVAARAHE
210 220 230 240 250
EDNTVELLRM SVDSRFRGKS IAKALGRRVL EFAMLHNYSA VVLGTTAVKV
260 270 280 290
AAHKLYESLG FRHMGASDHY VLPGMTLSLA ERLFFQVRYH RYRLQLREE
Length:299
Mass (Da):32,777
Last modified:October 11, 2005 - v1
Checksum:i488DC1B586265DAD
GO

Sequence cautioni

The sequence AAH75616.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence ABA54615.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti220 – 2201S → G in BAE28014 (PubMed:16141072).Curated
Sequence conflicti220 – 2201S → G in ABA54615 (PubMed:17626222).Curated
Sequence conflicti220 – 2201S → G in AAH75616 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147307 mRNA. Translation: BAE27836.1.
AK147594 mRNA. Translation: BAE28014.1.
AK147703 mRNA. Translation: BAE28084.1.
DQ174094 mRNA. Translation: ABA54615.1. Different initiation.
BC034068 mRNA. Translation: AAH34068.1.
BC075616 mRNA. Translation: AAH75616.1. Different initiation.
CCDSiCCDS19210.1.
RefSeqiNP_001001985.3. NM_001001985.3.
UniGeneiMm.274610.

Genome annotation databases

GeneIDi269642.
KEGGimmu:269642.
UCSCiuc008xbs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147307 mRNA. Translation: BAE27836.1.
AK147594 mRNA. Translation: BAE28014.1.
AK147703 mRNA. Translation: BAE28084.1.
DQ174094 mRNA. Translation: ABA54615.1. Different initiation.
BC034068 mRNA. Translation: AAH34068.1.
BC075616 mRNA. Translation: AAH75616.1. Different initiation.
CCDSiCCDS19210.1.
RefSeqiNP_001001985.3. NM_001001985.3.
UniGeneiMm.274610.

3D structure databases

ProteinModelPortaliQ3UGX3.
SMRiQ3UGX3. Positions 158-273.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059313.

Proteomic databases

MaxQBiQ3UGX3.
PaxDbiQ3UGX3.
PRIDEiQ3UGX3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi269642.
KEGGimmu:269642.
UCSCiuc008xbs.1. mouse.

Organism-specific databases

CTDi339983.
MGIiMGI:2447776. Nat8l.

Phylogenomic databases

eggNOGiENOG410IS8M. Eukaryota.
ENOG410YY26. LUCA.
HOGENOMiHOG000113735.
HOVERGENiHBG108173.
InParanoidiQ3UGX3.
KOiK18309.
OrthoDBiEOG73Z2W2.
PhylomeDBiQ3UGX3.
TreeFamiTF324687.

Enzyme and pathway databases

BRENDAi2.3.1.17. 3474.

Miscellaneous databases

ChiTaRSiNat8l. mouse.
NextBioi392959.
PROiQ3UGX3.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UGX3.
CleanExiMM_NAT8L.

Family and domain databases

Gene3Di3.40.630.30. 2 hits.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "A novel molecule 'Shati' is involved in methamphetamine-induced hyperlocomotion, sensitization, and conditioned place preference."
    Niwa M., Nitta A., Mizoguchi H., Ito Y., Noda Y., Nagai T., Nabeshima T.
    J. Neurosci. 27:7604-7615(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-299, IDENTIFICATION, TISSUE SPECIFICITY, INDUCTION BY METHAMPHETAMINE.
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 21-299.
    Strain: C57BL/6J and FVB/N.
    Tissue: Brain and Colon.
  4. "A novel molecule 'shati' increases dopamine uptake via the induction of tumor necrosis factor-alpha in pheochromocytoma-12 cells."
    Niwa M., Nitta A., Cen X., Kitaichi K., Ozaki N., Yamada K., Nabeshima T.
    J. Neurochem. 107:1697-1708(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Methamphetamine-induced neuronal protein NAT8L is the NAA biosynthetic enzyme: implications for specialized acetyl coenzyme A metabolism in the CNS."
    Ariyannur P.S., Moffett J.R., Manickam P., Pattabiraman N., Arun P., Nitta A., Nabeshima T., Madhavarao C.N., Namboodiri A.M.
    Brain Res. 1335:1-13(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "Molecular characterization of N-acetylaspartylglutamate synthetase."
    Becker I., Lodder J., Gieselmann V., Eckhardt M.
    J. Biol. Chem. 285:29156-29164(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNAT8L_MOUSE
AccessioniPrimary (citable) accession number: Q3UGX3
Secondary accession number(s): Q3UH43, Q6DID6, Q8K065
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 11, 2005
Last modified: November 11, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Seems to modulate behavioral effects induced by methamphetamine in vivo.
'Shati' is the name of the symbol at Nagoya castle in Japan.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.