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Protein

Amidophosphoribosyltransferase

Gene

Ppat

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Note: Binds 1 magnesium ion per subunit.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei12 – 121For GATase activityUniRule annotation
Metal bindingi280 – 2801Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi426 – 4261Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi503 – 5031Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi506 – 5061Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  2. iron-sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. nucleoside metabolic process Source: InterPro
  3. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

Iron, Iron-sulfurUniRule annotation, MagnesiumUniRule annotation, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Gene namesi
Name:PpatImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:2387203. Ppat.

Expressioni

Gene expression databases

GenevestigatoriQ3UGU3.

Structurei

3D structure databases

ProteinModelPortaliQ3UGU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Phylogenomic databases

HOVERGENiHBG002589.
KOiK00764.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UGU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELEELGIRE ECGVFGCIAS GDWPTQLDVP HVITLGLVGL QHRGQESAGI
60 70 80 90 100
VTSDGSAVPK FRVHKGMGLV NHVFTEDNLK KLYDSNLGIG HTRYATTGKC
110 120 130 140 150
ELENCQPFVV ETLHGKIAVA HNGELVNAAR LRKKLLRQGI GLSTSSDSEM
160 170 180 190 200
ITQLLAYTPP QEKDDAPDWV ARIKNLMKEA PAAYSLVIMH RDFIYAVRDP
210 220 230 240 250
YGNRPLCIGR LMPVSDVNDK EKKSSETEGW VVSSESCSFL SIGARYCHEV
260 270 280 290 300
KPGEIVEISR HGIRTLDIIP RSNGDPVAFC IFEYVYFARP DSMFEDQMVY
310 320 330 340 350
TVRYRCGQQL AIEAPVEADL VSTVPESATP AALGYATKCG LPYVEVLCKN
360 370 380 390 400
RYVGRTFIHP TLRLRQLGVA KKFGVSSDNF KGKRIVLIDD SIVRGNTISP
410 420 430 440 450
IIKLLKESGA KEVHIRVASP PIKYPCFMGI NIPTKEELIA NKPEFDCLAE
460 470 480 490 500
YLGANSVVYL SVEGLVSSVQ QEIKFKKQKV KKHDIAIQEN GNGLEYFEKT
510
GHCTACLTGQ YPVELEW
Length:517
Mass (Da):57,355
Last modified:October 10, 2005 - v1
Checksum:iFAA15F6386729DC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147750 mRNA. Translation: BAE28114.1.
RefSeqiNP_742158.1. NM_172146.2.
UniGeneiMm.202337.

Genome annotation databases

GeneIDi231327.
KEGGimmu:231327.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147750 mRNA. Translation: BAE28114.1.
RefSeqiNP_742158.1. NM_172146.2.
UniGeneiMm.202337.

3D structure databases

ProteinModelPortaliQ3UGU3.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC44.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi231327.
KEGGimmu:231327.

Organism-specific databases

CTDi5471.
MGIiMGI:2387203. Ppat.

Phylogenomic databases

HOVERGENiHBG002589.
KOiK00764.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Miscellaneous databases

NextBioi380505.
SOURCEiSearch...

Gene expression databases

GenevestigatoriQ3UGU3.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "High-efficiency full-length cDNA cloning."
    Carninci P., Hayashizaki Y.
    Methods Enzymol. 303:19-44(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
  2. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
    Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
    Genome Res. 10:1617-1630(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
  4. "Functional annotation of a full-length mouse cDNA collection."
    The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium
    Nature 409:685-690(2000)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
  5. "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
    The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
    Nature 420:563-573(2001)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
  6. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
  7. "The Transcriptional Landscape of the Mammalian Genome."
    The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
    Science 309:1559-1563(2004)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
  8. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.

Entry informationi

Entry nameiQ3UGU3_MOUSE
AccessioniPrimary (citable) accession number: Q3UGU3
Entry historyi
Integrated into UniProtKB/TrEMBL: October 10, 2005
Last sequence update: October 10, 2005
Last modified: March 31, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.