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Protein

Amidophosphoribosyltransferase

Gene

Ppat

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (Ppat), Amidophosphoribosyltransferase (Ppat), Amidophosphoribosyltransferase (Ppat), Amidophosphoribosyltransferase (Ppat)
  2. Trifunctional purine biosynthetic protein adenosine-3 (Gart), Trifunctional purine biosynthetic protein adenosine-3 (Gart)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12For GATase activityUniRule annotation1
Metal bindingi280Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi426Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi503Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi506Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferaseUniRule annotation, Transferase
Biological processPurine biosynthesisUniRule annotation
LigandIron, Iron-sulfurUniRule annotation, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
AmidophosphoribosyltransferaseUniRule annotation (EC:2.4.2.14UniRule annotation)
Short name:
ATaseUniRule annotation
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferaseUniRule annotation
Gene namesi
Name:PpatImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:2387203. Ppat.

PTM / Processingi

Proteomic databases

PaxDbiQ3UGU3.
PeptideAtlasiQ3UGU3.

Expressioni

Gene expression databases

GenevisibleiQ3UGU3. MM.

Structurei

3D structure databases

ProteinModelPortaliQ3UGU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 261Glutamine amidotransferase type-2InterPro annotationAdd BLAST250

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0572. Eukaryota.
COG0034. LUCA.
HOVERGENiHBG002589.
KOiK00764.

Family and domain databases

CDDicd00715. GPATase_N. 1 hit.
cd06223. PRTases_typeI. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR005854. PurF.
IPR035584. PurF_N.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3UGU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELEELGIRE ECGVFGCIAS GDWPTQLDVP HVITLGLVGL QHRGQESAGI
60 70 80 90 100
VTSDGSAVPK FRVHKGMGLV NHVFTEDNLK KLYDSNLGIG HTRYATTGKC
110 120 130 140 150
ELENCQPFVV ETLHGKIAVA HNGELVNAAR LRKKLLRQGI GLSTSSDSEM
160 170 180 190 200
ITQLLAYTPP QEKDDAPDWV ARIKNLMKEA PAAYSLVIMH RDFIYAVRDP
210 220 230 240 250
YGNRPLCIGR LMPVSDVNDK EKKSSETEGW VVSSESCSFL SIGARYCHEV
260 270 280 290 300
KPGEIVEISR HGIRTLDIIP RSNGDPVAFC IFEYVYFARP DSMFEDQMVY
310 320 330 340 350
TVRYRCGQQL AIEAPVEADL VSTVPESATP AALGYATKCG LPYVEVLCKN
360 370 380 390 400
RYVGRTFIHP TLRLRQLGVA KKFGVSSDNF KGKRIVLIDD SIVRGNTISP
410 420 430 440 450
IIKLLKESGA KEVHIRVASP PIKYPCFMGI NIPTKEELIA NKPEFDCLAE
460 470 480 490 500
YLGANSVVYL SVEGLVSSVQ QEIKFKKQKV KKHDIAIQEN GNGLEYFEKT
510
GHCTACLTGQ YPVELEW
Length:517
Mass (Da):57,355
Last modified:October 11, 2005 - v1
Checksum:iFAA15F6386729DC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147750 mRNA. Translation: BAE28114.1.
RefSeqiNP_742158.1. NM_172146.2.
UniGeneiMm.202337.

Genome annotation databases

GeneIDi231327.
KEGGimmu:231327.

Similar proteinsi

Entry informationi

Entry nameiQ3UGU3_MOUSE
AccessioniPrimary (citable) accession number: Q3UGU3
Entry historyiIntegrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: August 30, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)