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Q3UGU3 (Q3UGU3_MOUSE) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485

Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485
Gene names
Name:Ppat MGI 2387203
OrganismMus musculus (Mouse) EMBL BAE28114.1
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3

Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site121For GATase activity By similarity PIRSR PIRSR000485-1
Metal binding2801Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding4261Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding5031Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
Metal binding5061Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

Sequence LengthMass (Da)Tools
Q3UGU3 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: FAA15F6386729DC1

FASTA51757,355
        10         20         30         40         50         60 
MELEELGIRE ECGVFGCIAS GDWPTQLDVP HVITLGLVGL QHRGQESAGI VTSDGSAVPK 

        70         80         90        100        110        120 
FRVHKGMGLV NHVFTEDNLK KLYDSNLGIG HTRYATTGKC ELENCQPFVV ETLHGKIAVA 

       130        140        150        160        170        180 
HNGELVNAAR LRKKLLRQGI GLSTSSDSEM ITQLLAYTPP QEKDDAPDWV ARIKNLMKEA 

       190        200        210        220        230        240 
PAAYSLVIMH RDFIYAVRDP YGNRPLCIGR LMPVSDVNDK EKKSSETEGW VVSSESCSFL 

       250        260        270        280        290        300 
SIGARYCHEV KPGEIVEISR HGIRTLDIIP RSNGDPVAFC IFEYVYFARP DSMFEDQMVY 

       310        320        330        340        350        360 
TVRYRCGQQL AIEAPVEADL VSTVPESATP AALGYATKCG LPYVEVLCKN RYVGRTFIHP 

       370        380        390        400        410        420 
TLRLRQLGVA KKFGVSSDNF KGKRIVLIDD SIVRGNTISP IIKLLKESGA KEVHIRVASP 

       430        440        450        460        470        480 
PIKYPCFMGI NIPTKEELIA NKPEFDCLAE YLGANSVVYL SVEGLVSSVQ QEIKFKKQKV 

       490        500        510 
KKHDIAIQEN GNGLEYFEKT GHCTACLTGQ YPVELEW 

« Hide

References

[1]"High-efficiency full-length cDNA cloning."
Carninci P., Hayashizaki Y.
Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J EMBL BAE28114.1.
[2]"Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J EMBL BAE28114.1.
[3]"RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer."
Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P., Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N., Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A. expand/collapse author list , Yamamoto R., Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S., Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y., Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y., Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.
Genome Res. 10:1757-1771(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J EMBL BAE28114.1.
[4]"Functional annotation of a full-length mouse cDNA collection."
The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium
Nature 409:685-690(2001)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J EMBL BAE28114.1.
[5]"Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
Nature 420:563-573(2002)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J EMBL BAE28114.1.
[6]Arakawa T., Carninci P., Fukuda S., Hashizume W., Hayashida K., Hori F., Iida J., Imamura K., Imotani K., Itoh M., Kanagawa S., Kawai J., Kojima M., Konno H., Murata M., Nakamura M., Ninomiya N., Nishiyori H. expand/collapse author list , Nomura K., Ohno M., Sakazume N., Sano H., Sasaki D., Shibata K., Shiraki T., Tagami M., Tagami Y., Waki K., Watahiki A., Muramatsu M., Hayashizaki Y.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J EMBL BAE28114.1.
[7]"The Transcriptional Landscape of the Mammalian Genome."
The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
Science 309:1559-1563(2005)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J EMBL BAE28114.1.
[8]"Antisense Transcription in the Mammalian Transcriptome."
RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium
Science 309:1564-1566(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C57BL/6J EMBL BAE28114.1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK147750 mRNA. Translation: BAE28114.1.
RefSeqNP_742158.1. NM_172146.2.
UniGeneMm.202337.

3D structure databases

ProteinModelPortalQ3UGU3.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID231327.
KEGGmmu:231327.

Organism-specific databases

CTD5471.
MGIMGI:2387203. Ppat.

Phylogenomic databases

HOVERGENHBG002589.
InParanoidQ3UGU3.
KOK00764.

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Gene expression databases

GenevestigatorQ3UGU3.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00310. GATase_2. 2 hits.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio380505.
SOURCESearch...

Entry information

Entry nameQ3UGU3_MOUSE
AccessionPrimary (citable) accession number: Q3UGU3
Entry history
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)