Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase

Gene

Alg10b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative alpha-1,2-glucosyltransferase, which adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol (By similarity). When coupled to KCNH2 may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by mediating glycosylation of KCNH2 (By similarity). Has a role in maintenance of cochlear outer hair cell function (PubMed:24303013).By similarity1 Publication

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->2)-D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.By similarity

GO - Molecular functioni

GO - Biological processi

  • auditory receptor cell development Source: MGI
  • dolichol-linked oligosaccharide biosynthetic process Source: InterPro
  • sensory perception of sound Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Protein family/group databases

CAZyiGT59. Glycosyltransferase Family 59.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseBy similarity (EC:2.4.1.256By similarity)
Alternative name(s):
Alpha-1,2-glucosyltransferase ALG10-A
Alpha-2-glucosyltransferase ALG10-B
Asparagine-linked glycosylation protein 10 homolog B
Gene namesi
Name:Alg10b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:2146159. Alg10b.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 64ExtracellularSequence analysisAdd BLAST37
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21
Topological domaini86 – 97CytoplasmicSequence analysisAdd BLAST12
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 126ExtracellularSequence analysis8
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Topological domaini148 – 150CytoplasmicSequence analysis3
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 175ExtracellularSequence analysis4
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 256CytoplasmicSequence analysisAdd BLAST60
Transmembranei257 – 277HelicalSequence analysisAdd BLAST21
Topological domaini278 – 283ExtracellularSequence analysis6
Transmembranei284 – 304HelicalSequence analysisAdd BLAST21
Topological domaini305 – 317CytoplasmicSequence analysisAdd BLAST13
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Topological domaini339 – 365ExtracellularSequence analysisAdd BLAST27
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 392CytoplasmicSequence analysis6
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 436ExtracellularSequence analysisAdd BLAST23
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Topological domaini458 – 473CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi389L → S: Significant hearing impairment, associated with loss or degeneration of cochlear outer hair cells. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003200641 – 474Putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferaseAdd BLAST474

Proteomic databases

MaxQBiQ3UGP8.
PaxDbiQ3UGP8.
PeptideAtlasiQ3UGP8.
PRIDEiQ3UGP8.

PTM databases

iPTMnetiQ3UGP8.
PhosphoSitePlusiQ3UGP8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000075470.
GenevisibleiQ3UGP8. MM.

Interactioni

Subunit structurei

Interacts with KCNH1 and KCNH2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000097882.

Structurei

3D structure databases

ProteinModelPortaliQ3UGP8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALG10 glucosyltransferase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2642. Eukaryota.
ENOG410YGXE. LUCA.
GeneTreeiENSGT00390000012906.
HOGENOMiHOG000240902.
HOVERGENiHBG053222.
InParanoidiQ3UGP8.
KOiK03850.
OMAiRKVQPRH.
OrthoDBiEOG091G0A27.
PhylomeDBiQ3UGP8.
TreeFamiTF300150.

Family and domain databases

InterProiIPR016900. Alg10.
[Graphical view]
PANTHERiPTHR12989. PTHR12989. 1 hit.
PfamiPF04922. DIE2_ALG10. 1 hit.
[Graphical view]
PIRSFiPIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3UGP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLEGYYFS AALSCTFLVS CLLFSAFSRA LREPYMDEIF HLPQAQRYCE
60 70 80 90 100
GRFSLSQWDP MITTLPGLYL VSVGVVKPAS WLLGWSEHVI CSIGVLRFVN
110 120 130 140 150
LLFSVGNFYL LYLLFRKVQP RNKASSSIQR ILSTLTLAVF PTLYFFNFLY
160 170 180 190 200
YTEAGSVFFT LFAYLMCLYG NHRTSALLGF CGFMFRQTNI IWAAFCAGHL
210 220 230 240 250
IAQKCSEAWK IELQKKKEER LAPTKGPLSE LRRVLQFLLV YAMSLKNLRM
260 270 280 290 300
LFLLTWPYVL LLLAFFAFVV VNGGIVVGDR SSHEACLHFP QLFYFFSFTA
310 320 330 340 350
FFSFPHLLSL TKVKTFLSLV WKRRVQFSVV TLVSILLVWK FTYVHKYLLA
360 370 380 390 400
DNRHYTFYVW KRVFQRHEVV KYLLVPAYIF AGWAIADSLK AKSIFWNLMF
410 420 430 440 450
FVCLVASTVP QKLLEFRYFI LPYIIYRLNI PLPPISRLVC ELGCYTVVNF
460 470
VTFYIFLNKT FQWPNSQDIQ RFMW
Length:474
Mass (Da):55,465
Last modified:October 11, 2005 - v1
Checksum:i8D18F21134AC089F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti211I → T in BAE38718 (PubMed:16141072).Curated1
Sequence conflicti221L → F in BAE38718 (PubMed:16141072).Curated1
Sequence conflicti240V → L in BAE38718 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147821 mRNA. Translation: BAE28159.1.
AK166344 mRNA. Translation: BAE38718.1.
CCDSiCCDS27757.1.
RefSeqiNP_001028613.1. NM_001033441.3.
UniGeneiMm.17853.

Genome annotation databases

EnsembliENSMUST00000100309; ENSMUSP00000097882; ENSMUSG00000075470.
GeneIDi380959.
KEGGimmu:380959.
UCSCiuc007xhj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147821 mRNA. Translation: BAE28159.1.
AK166344 mRNA. Translation: BAE38718.1.
CCDSiCCDS27757.1.
RefSeqiNP_001028613.1. NM_001033441.3.
UniGeneiMm.17853.

3D structure databases

ProteinModelPortaliQ3UGP8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000097882.

Protein family/group databases

CAZyiGT59. Glycosyltransferase Family 59.

PTM databases

iPTMnetiQ3UGP8.
PhosphoSitePlusiQ3UGP8.

Proteomic databases

MaxQBiQ3UGP8.
PaxDbiQ3UGP8.
PeptideAtlasiQ3UGP8.
PRIDEiQ3UGP8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100309; ENSMUSP00000097882; ENSMUSG00000075470.
GeneIDi380959.
KEGGimmu:380959.
UCSCiuc007xhj.1. mouse.

Organism-specific databases

CTDi144245.
MGIiMGI:2146159. Alg10b.

Phylogenomic databases

eggNOGiKOG2642. Eukaryota.
ENOG410YGXE. LUCA.
GeneTreeiENSGT00390000012906.
HOGENOMiHOG000240902.
HOVERGENiHBG053222.
InParanoidiQ3UGP8.
KOiK03850.
OMAiRKVQPRH.
OrthoDBiEOG091G0A27.
PhylomeDBiQ3UGP8.
TreeFamiTF300150.

Miscellaneous databases

PROiQ3UGP8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000075470.
GenevisibleiQ3UGP8. MM.

Family and domain databases

InterProiIPR016900. Alg10.
[Graphical view]
PANTHERiPTHR12989. PTHR12989. 1 hit.
PfamiPF04922. DIE2_ALG10. 1 hit.
[Graphical view]
PIRSFiPIRSF028810. Alpha1_2_glucosyltferase_Alg10. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAG10B_MOUSE
AccessioniPrimary (citable) accession number: Q3UGP8
Secondary accession number(s): Q3TLS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.