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Q3UGC7

- EI3JA_MOUSE

UniProt

Q3UGC7 - EI3JA_MOUSE

Protein

Eukaryotic translation initiation factor 3 subunit J-A

Gene

Eif3j1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.By similarityUniRule annotation

    GO - Molecular functioni

    1. translation initiation factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. formation of translation preinitiation complex Source: UniProtKB-HAMAP
    2. regulation of translational initiation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 3 subunit J-AUniRule annotation
    Short name:
    eIF3j-AUniRule annotation
    Alternative name(s):
    Eukaryotic translation initiation factor 3 subunit 1-AUniRule annotation
    eIF-3-alpha-AUniRule annotation
    eIF3 p35UniRule annotation
    Gene namesi
    Name:Eif3j1
    Synonyms:Eif3s1-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1925905. Eif3j1.

    Subcellular locationi

    Cytoplasm By similarityUniRule annotation

    GO - Cellular componenti

    1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
    2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
    3. eukaryotic translation initiation factor 3 complex Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Eukaryotic translation initiation factor 3 subunit J-APRO_0000419334Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141PhosphoserineBy similarityUniRule annotation
    Modified residuei16 – 161PhosphoserineBy similarityUniRule annotation
    Modified residuei23 – 231PhosphoserineBy similarityUniRule annotation
    Modified residuei112 – 1121PhosphothreonineBy similarityUniRule annotation
    Modified residuei130 – 1301PhosphoserineBy similarityUniRule annotation
    Modified residuei257 – 2571PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated. Phosphorylation is enhanced upon serum stimulation.By similarityUniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ3UGC7.
    PaxDbiQ3UGC7.
    PRIDEiQ3UGC7.

    Expressioni

    Gene expression databases

    BgeeiQ3UGC7.
    GenevestigatoriQ3UGC7.

    Interactioni

    Subunit structurei

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.By similarityUniRule annotation

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000028668.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3UGC7.
    SMRiQ3UGC7. Positions 145-214.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 7269Sufficient for interaction with EIF3BUniRule annotationAdd
    BLAST
    Regioni246 – 26116Promotes stable association with the 40S ribosomeUniRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili73 – 13866UniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the eIF-3 subunit J family.UniRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG247523.
    GeneTreeiENSGT00390000018400.
    HOGENOMiHOG000238746.
    HOVERGENiHBG066230.
    InParanoidiQ3UGC7.
    KOiK03245.
    OMAiSTCGIDA.
    OrthoDBiEOG7J70H8.
    PhylomeDBiQ3UGC7.
    TreeFamiTF101514.

    Family and domain databases

    Gene3Di1.10.246.60. 1 hit.
    HAMAPiMF_03009. eIF3j.
    InterProiIPR023194. eIF3-like_dom.
    IPR013906. eIF3j.
    [Graphical view]
    PANTHERiPTHR21681. PTHR21681. 1 hit.
    PfamiPF08597. eIF3_subunit. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3UGC7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAAAAAAAA AGDSDSWDAD TFSMEDPVRK VAGGGTAGGD RWEGEDEDED    50
    VKDNWDDDDD ENKEEAEVKP EVKISEKKKI AEKIKEKERQ QKKRQEEIKK 100
    RLEEPEESKV LTPEEQLADK LRLKKLQEES DLELAKETFG VNNTVYGIDA 150
    MNPSSRDDFT EFGKLLKDKI TQYEKSLYYA SFLEALVRDV CISLEIDDLK 200
    KITNSLTVLC SEKQKQEKQS KAKKKKKGVV PGGGLKATMK DDLADYGGYE 250
    GGYVQDYEDF M 261
    Length:261
    Mass (Da):29,344
    Last modified:October 11, 2005 - v1
    Checksum:i5BED22967289E651
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK147154 mRNA. Translation: BAE27721.1.
    AK148004 mRNA. Translation: BAE28282.1.
    AK154934 mRNA. Translation: BAE32935.1.
    AK161038 mRNA. Translation: BAE36162.1.
    AL845457 Genomic DNA. Translation: CAM21780.1.
    CH466519 Genomic DNA. Translation: EDL28081.1.
    CCDSiCCDS38219.1.
    RefSeqiNP_653128.2. NM_144545.4.
    XP_006500478.1. XM_006500415.1.
    UniGeneiMm.458184.

    Genome annotation databases

    EnsembliENSMUST00000028668; ENSMUSP00000028668; ENSMUSG00000027236.
    GeneIDi78655.
    KEGGimmu:78655.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK147154 mRNA. Translation: BAE27721.1 .
    AK148004 mRNA. Translation: BAE28282.1 .
    AK154934 mRNA. Translation: BAE32935.1 .
    AK161038 mRNA. Translation: BAE36162.1 .
    AL845457 Genomic DNA. Translation: CAM21780.1 .
    CH466519 Genomic DNA. Translation: EDL28081.1 .
    CCDSi CCDS38219.1.
    RefSeqi NP_653128.2. NM_144545.4.
    XP_006500478.1. XM_006500415.1.
    UniGenei Mm.458184.

    3D structure databases

    ProteinModelPortali Q3UGC7.
    SMRi Q3UGC7. Positions 145-214.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000028668.

    Proteomic databases

    MaxQBi Q3UGC7.
    PaxDbi Q3UGC7.
    PRIDEi Q3UGC7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028668 ; ENSMUSP00000028668 ; ENSMUSG00000027236 .
    GeneIDi 78655.
    KEGGi mmu:78655.

    Organism-specific databases

    CTDi 78655.
    MGIi MGI:1925905. Eif3j1.

    Phylogenomic databases

    eggNOGi NOG247523.
    GeneTreei ENSGT00390000018400.
    HOGENOMi HOG000238746.
    HOVERGENi HBG066230.
    InParanoidi Q3UGC7.
    KOi K03245.
    OMAi STCGIDA.
    OrthoDBi EOG7J70H8.
    PhylomeDBi Q3UGC7.
    TreeFami TF101514.

    Miscellaneous databases

    NextBioi 349286.
    PROi Q3UGC7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3UGC7.
    Genevestigatori Q3UGC7.

    Family and domain databases

    Gene3Di 1.10.246.60. 1 hit.
    HAMAPi MF_03009. eIF3j.
    InterProi IPR023194. eIF3-like_dom.
    IPR013906. eIF3j.
    [Graphical view ]
    PANTHERi PTHR21681. PTHR21681. 1 hit.
    Pfami PF08597. eIF3_subunit. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6JImported and NODImported.
      Tissue: AmnionImported, Dendritic cellImported, Embryonic liverImported and MelanocyteImported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6JImported.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiEI3JA_MOUSE
    AccessioniPrimary (citable) accession number: Q3UGC7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2012
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3