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Protein

Eukaryotic translation initiation factor 3 subunit J-A

Gene

Eif3j1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.By similarityUniRule annotation

GO - Molecular functioni

  1. translation initiation factor activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. formation of translation preinitiation complex Source: UniProtKB-HAMAP
  2. regulation of translational initiation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

ReactomeiREACT_236458. Ribosomal scanning and start codon recognition.
REACT_249044. Formation of a pool of free 40S subunits.
REACT_249316. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_256488. Translation initiation complex formation.
REACT_259469. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_262078. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit J-AUniRule annotation
Short name:
eIF3j-AUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 1-AUniRule annotation
eIF-3-alpha-AUniRule annotation
eIF3 p35UniRule annotation
Gene namesi
Name:Eif3j1
Synonyms:Eif3s1-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1925905. Eif3j1.

Subcellular locationi

Cytoplasm By similarityUniRule annotation

GO - Cellular componenti

  1. eukaryotic 43S preinitiation complex Source: UniProtKB-HAMAP
  2. eukaryotic 48S preinitiation complex Source: UniProtKB-HAMAP
  3. eukaryotic translation initiation factor 3 complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Eukaryotic translation initiation factor 3 subunit J-APRO_0000419334Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarityUniRule annotation
Modified residuei16 – 161PhosphoserineBy similarityUniRule annotation
Modified residuei23 – 231PhosphoserineBy similarityUniRule annotation
Modified residuei112 – 1121PhosphothreonineBy similarityUniRule annotation
Modified residuei130 – 1301PhosphoserineBy similarityUniRule annotation
Modified residuei257 – 2571PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.By similarityUniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UGC7.
PaxDbiQ3UGC7.
PRIDEiQ3UGC7.

Expressioni

Gene expression databases

BgeeiQ3UGC7.
ExpressionAtlasiQ3UGC7. baseline.
GenevestigatoriQ3UGC7.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.By similarityUniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028668.

Structurei

3D structure databases

ProteinModelPortaliQ3UGC7.
SMRiQ3UGC7. Positions 145-214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 7269Sufficient for interaction with EIF3BUniRule annotationAdd
BLAST
Regioni246 – 26116Promotes stable association with the 40S ribosomeUniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili73 – 13866UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-3 subunit J family.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG247523.
GeneTreeiENSGT00390000018400.
HOGENOMiHOG000238746.
HOVERGENiHBG066230.
InParanoidiQ3UGC7.
KOiK03245.
OMAiSTCGIDA.
OrthoDBiEOG7J70H8.
PhylomeDBiQ3UGC7.
TreeFamiTF101514.

Family and domain databases

Gene3Di1.10.246.60. 1 hit.
HAMAPiMF_03009. eIF3j.
InterProiIPR023194. eIF3-like_dom.
IPR013906. eIF3j.
[Graphical view]
PANTHERiPTHR21681. PTHR21681. 1 hit.
PfamiPF08597. eIF3_subunit. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UGC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAAAAAA AGDSDSWDAD TFSMEDPVRK VAGGGTAGGD RWEGEDEDED
60 70 80 90 100
VKDNWDDDDD ENKEEAEVKP EVKISEKKKI AEKIKEKERQ QKKRQEEIKK
110 120 130 140 150
RLEEPEESKV LTPEEQLADK LRLKKLQEES DLELAKETFG VNNTVYGIDA
160 170 180 190 200
MNPSSRDDFT EFGKLLKDKI TQYEKSLYYA SFLEALVRDV CISLEIDDLK
210 220 230 240 250
KITNSLTVLC SEKQKQEKQS KAKKKKKGVV PGGGLKATMK DDLADYGGYE
260
GGYVQDYEDF M
Length:261
Mass (Da):29,344
Last modified:October 11, 2005 - v1
Checksum:i5BED22967289E651
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147154 mRNA. Translation: BAE27721.1.
AK148004 mRNA. Translation: BAE28282.1.
AK154934 mRNA. Translation: BAE32935.1.
AK161038 mRNA. Translation: BAE36162.1.
AL845457 Genomic DNA. Translation: CAM21780.1.
CH466519 Genomic DNA. Translation: EDL28081.1.
CCDSiCCDS38219.1.
RefSeqiNP_653128.2. NM_144545.4.
XP_006500478.1. XM_006500415.1.
UniGeneiMm.458184.

Genome annotation databases

EnsembliENSMUST00000028668; ENSMUSP00000028668; ENSMUSG00000027236.
GeneIDi78655.
KEGGimmu:78655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK147154 mRNA. Translation: BAE27721.1.
AK148004 mRNA. Translation: BAE28282.1.
AK154934 mRNA. Translation: BAE32935.1.
AK161038 mRNA. Translation: BAE36162.1.
AL845457 Genomic DNA. Translation: CAM21780.1.
CH466519 Genomic DNA. Translation: EDL28081.1.
CCDSiCCDS38219.1.
RefSeqiNP_653128.2. NM_144545.4.
XP_006500478.1. XM_006500415.1.
UniGeneiMm.458184.

3D structure databases

ProteinModelPortaliQ3UGC7.
SMRiQ3UGC7. Positions 145-214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028668.

Proteomic databases

MaxQBiQ3UGC7.
PaxDbiQ3UGC7.
PRIDEiQ3UGC7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028668; ENSMUSP00000028668; ENSMUSG00000027236.
GeneIDi78655.
KEGGimmu:78655.

Organism-specific databases

CTDi78655.
MGIiMGI:1925905. Eif3j1.

Phylogenomic databases

eggNOGiNOG247523.
GeneTreeiENSGT00390000018400.
HOGENOMiHOG000238746.
HOVERGENiHBG066230.
InParanoidiQ3UGC7.
KOiK03245.
OMAiSTCGIDA.
OrthoDBiEOG7J70H8.
PhylomeDBiQ3UGC7.
TreeFamiTF101514.

Enzyme and pathway databases

ReactomeiREACT_236458. Ribosomal scanning and start codon recognition.
REACT_249044. Formation of a pool of free 40S subunits.
REACT_249316. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_256488. Translation initiation complex formation.
REACT_259469. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_262078. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

NextBioi349286.
PROiQ3UGC7.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UGC7.
ExpressionAtlasiQ3UGC7. baseline.
GenevestigatoriQ3UGC7.

Family and domain databases

Gene3Di1.10.246.60. 1 hit.
HAMAPiMF_03009. eIF3j.
InterProiIPR023194. eIF3-like_dom.
IPR013906. eIF3j.
[Graphical view]
PANTHERiPTHR21681. PTHR21681. 1 hit.
PfamiPF08597. eIF3_subunit. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported and NODImported.
    Tissue: AmnionImported, Dendritic cellImported, Embryonic liverImported and MelanocyteImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6JImported.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiEI3JA_MOUSE
AccessioniPrimary (citable) accession number: Q3UGC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: October 11, 2005
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.