Reviewed, UniProtKB/Swiss-Prot Q3UFY7 (5NT3L_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytosolic 5'-nucleotidase III-like protein
      Short name=cN-III-like protein
    EC=3.1.3.5

Gene names

Name:

Nt5c3l

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Can act both as nucleotidase and as phosphotransferase By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the pyrimidine 5'-nucleotidase family.
Sequence caution	The sequence BAB24814.1 differs from that shown. Reason: Frameshift at position 10. The sequence CAM23034.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAM23036.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase Transferase
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3UFY7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3UFY7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     249-253: VEERR → LPEAQ
     254-292: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q3UFY7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     69-77: LTELFHHYY → CGETCNPRG
     78-292: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Cytosolic 5'-nucleotidase III-like protein		PRO_0000328949
Regions
	Region	148 – 149	2	Substrate binding By similarity
Sites
	Active site	33	1	Nucleophile By similarity
	Active site	35	1	Proton donor By similarity
	Metal binding	33	1	Magnesium By similarity
	Metal binding	35	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	222	1	Magnesium By similarity
	Binding site	197	1	Substrate By similarity
Natural variations
	Alternative sequence	69 – 77	9	LTELFHHYY → CGETCNPRG in isoform 3.		VSP_032851
	Alternative sequence	78 – 292	215	Missing in isoform 3.		VSP_032852
	Alternative sequence	249 – 253	5	VEERR → LPEAQ in isoform 2.		VSP_032853
	Alternative sequence	254 – 292	39	Missing in isoform 2.		VSP_032854
Experimental info
	Sequence conflict	150	1	G → R in BAB24814. Ref.1
	Sequence conflict	177	1	D → H in BAB24814. Ref.1
	Sequence conflict	181	1	D → H in BAB24814. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 8, 2008. Version 2. Checksum: 3AE26BCE06833085	FASTA	292	33,578
	10 20 30 40 50 60 MKATVLMRQP GRVQEIVGAL RRGGGDRLQV ISDFDMTLSR FAYNGQRCPS SHNILDNSKI 70 80 90 100 110 120 ISEDCRKELT ELFHHYYPIE IDPHRTIKEK LPHMVQWWSK AHSLLCQQRI QKVQIAQVVG 130 140 150 160 170 180 ESTAMLREGY KTFFDTLYQN NIPLFIFSAG IGDILEEIIR QMKVFHPNIH IVSNYMDFSE 190 200 210 220 230 240 DGFLKGFKGQ LIHTYNKNSS VCENSSYFQQ LQNKTNIILL GDSIGDLTMA DGVPGVQNIL 250 260 270 280 290 KIGFLNDKVE ERRERYMDSY DIVLEKDETL DVVNGLLRHI LYQGDCVELQ GS
	Isoform 2. Checksum: ED42CD971AD0AF4B	FASTA	253	28,935
	10 20 30 40 50 60 MKATVLMRQP GRVQEIVGAL RRGGGDRLQV ISDFDMTLSR FAYNGQRCPS SHNILDNSKI 70 80 90 100 110 120 ISEDCRKELT ELFHHYYPIE IDPHRTIKEK LPHMVQWWSK AHSLLCQQRI QKVQIAQVVG 130 140 150 160 170 180 ESTAMLREGY KTFFDTLYQN NIPLFIFSAG IGDILEEIIR QMKVFHPNIH IVSNYMDFSE 190 200 210 220 230 240 DGFLKGFKGQ LIHTYNKNSS VCENSSYFQQ LQNKTNIILL GDSIGDLTMA DGVPGVQNIL 250 KIGFLNDKLP EAQ
	Isoform 3. Checksum: 9E0B871DFB76F24E	FASTA	77	8,585
	10 20 30 40 50 60 MKATVLMRQP GRVQEIVGAL RRGGGDRLQV ISDFDMTLSR FAYNGQRCPS SHNILDNSKI 70 ISEDCRKECG ETCNPRG

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J. Tissue: Testis and Thymus.
[2]	The mouse genome sequencing consortium Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK006972 mRNA. Translation: BAB24814.1. Frameshift. AK082816 mRNA. Translation: BAC38632.1. Different initiation. AK148222 mRNA. Translation: BAE28422.1. Different initiation. AK162770 mRNA. Translation: BAE37053.1. Different initiation. AK166393 mRNA. Translation: BAE38749.1. Different initiation. AL590968 Genomic DNA. Translation: CAM23034.1. Sequence problems. AL590968 Genomic DNA. Translation: CAM23035.1. AL590968 Genomic DNA. Translation: CAM23036.1. Sequence problems. BC015307 mRNA. Translation: AAH15307.1. Different initiation.
IPI	IPI00308345. IPI00649681. IPI00652301.
RefSeq	NP_001096120.1. NP_080837.3.
UniGene	Mm.28738
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q3UFY7.
Genome annotation databases
Ensembl	ENSMUSG00000017176. Mus musculus. [Contig view]
GeneID	68106.
KEGG	mmu:68106.
Organism-specific databases
MGI	MGI:1915356. Nt5c3l.
Phylogenomic databases
HOGENOM	Q3UFY7.
HOVERGEN	Q3UFY7.
Enzyme and pathway databases
BRENDA	3.1.3.5. 244.
Gene expression databases
ArrayExpress	Q3UFY7.
Bgee	Q3UFY7.
Family and domain databases
InterPro	IPR006434. HAD-SF_hydro_IE. IPR018012. HAD-SF_hydro_IE_pln. [Graphical view]
Pfam	PF05822. UMPH-1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01544. HAD-SF-IE. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	326426.
SOURCE	Search...

Entry information

Entry name

5NT3L_MOUSE

Accession

Primary (citable) accession number: Q3UFY7
Secondary accession number(s): A2A4I2 , A2A4I3, Q3TLP3, Q8BHU9, Q91WE8, Q9D9F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	June 16, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)