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Protein

7-methylguanosine phosphate-specific 5'-nucleotidase

Gene

Nt5c3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity).By similarity

Catalytic activityi

N(7)-methyl-GMP + H2O = N(7)-methyl-guanosine + phosphate.By similarity
CMP + H2O = cytidine + phosphate.By similarity
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei41 – 411NucleophileBy similarity
Metal bindingi41 – 411MagnesiumBy similarity
Active sitei43 – 431Proton donorBy similarity
Metal bindingi43 – 431Magnesium; via carbonyl oxygenBy similarity
Binding sitei88 – 881CMPBy similarity
Binding sitei88 – 881N(7)-methyl-GMPBy similarity
Binding sitei205 – 2051SubstrateBy similarity
Metal bindingi230 – 2301MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-429958. mRNA decay by 3' to 5' exoribonuclease.

Names & Taxonomyi

Protein namesi
Recommended name:
7-methylguanosine phosphate-specific 5'-nucleotidaseBy similarity (EC:3.1.3.91By similarity)
Short name:
7-methylguanosine nucleotidase
Alternative name(s):
Cytosolic 5'-nucleotidase 3B
Cytosolic 5'-nucleotidase III-like proteinBy similarity (EC:3.1.3.5By similarity)
Short name:
cN-III-like protein
N(7)-methylguanylate 5'-phosphatase
Gene namesi
Name:Nt5c3b
Synonyms:Nt5c3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1915356. Nt5c3b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3003007-methylguanosine phosphate-specific 5'-nucleotidasePRO_0000328949Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei256 – 2561N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ3UFY7.
MaxQBiQ3UFY7.
PaxDbiQ3UFY7.
PRIDEiQ3UFY7.

PTM databases

PhosphoSiteiQ3UFY7.

Expressioni

Gene expression databases

BgeeiQ3UFY7.
GenevisibleiQ3UFY7. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ3UFY7. 1 interaction.
MINTiMINT-4121322.
STRINGi10090.ENSMUSP00000090360.

Structurei

3D structure databases

ProteinModelPortaliQ3UFY7.
SMRiQ3UFY7. Positions 18-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni156 – 1572Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the pyrimidine 5'-nucleotidase family.Curated

Phylogenomic databases

eggNOGiKOG3128. Eukaryota.
ENOG410ZQJ8. LUCA.
GeneTreeiENSGT00390000012959.
HOVERGENiHBG059750.
InParanoidiQ3UFY7.
KOiK01081.
OMAiIISEECR.
OrthoDBiEOG7XSTHV.
TreeFamiTF314663.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view]
PfamiPF05822. UMPH-1. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01544. HAD-SF-IE. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UFY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEEVSSLMK ATVLMRQPGR VQEIVGALRR GGGDRLQVIS DFDMTLSRFA
60 70 80 90 100
YNGQRCPSSH NILDNSKIIS EDCRKELTEL FHHYYPIEID PHRTIKEKLP
110 120 130 140 150
HMVQWWSKAH SLLCQQRIQK VQIAQVVGES TAMLREGYKT FFDTLYQNNI
160 170 180 190 200
PLFIFSAGIG DILEEIIRQM KVFHPNIHIV SNYMDFSEDG FLKGFKGQLI
210 220 230 240 250
HTYNKNSSVC ENSSYFQQLQ NKTNIILLGD SIGDLTMADG VPGVQNILKI
260 270 280 290 300
GFLNDKVEER RERYMDSYDI VLEKDETLDV VNGLLRHILY QGDCVELQGS
Length:300
Mass (Da):34,425
Last modified:April 3, 2013 - v3
Checksum:i9208C1438FBFD2A2
GO
Isoform 2 (identifier: Q3UFY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     257-261: VEERR → LPEAQ
     262-300: Missing.

Note: No experimental confirmation available.
Show »
Length:261
Mass (Da):29,782
Checksum:i8518D31B3AE3A9DD
GO
Isoform 3 (identifier: Q3UFY7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     77-85: LTELFHHYY → CGETCNPRG
     86-300: Missing.

Note: No experimental confirmation available.
Show »
Length:85
Mass (Da):9,432
Checksum:iEAC65C5C364AF0B9
GO
Isoform 4 (identifier: Q3UFY7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.

Show »
Length:292
Mass (Da):33,578
Checksum:i3AE26BCE06833085
GO

Sequence cautioni

The sequence AAH15307.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB24814.1 differs from that shown. Reason: Frameshift at position 18. Curated
The sequence BAC38632.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE37053.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAM23034.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAM23036.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti158 – 1581G → R in BAB24814 (PubMed:16141072).Curated
Sequence conflicti185 – 1851D → H in BAB24814 (PubMed:16141072).Curated
Sequence conflicti189 – 1891D → H in BAB24814 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 88Missing in isoform 4. 1 PublicationVSP_046298
Alternative sequencei77 – 859LTELFHHYY → CGETCNPRG in isoform 3. 1 PublicationVSP_032851
Alternative sequencei86 – 300215Missing in isoform 3. 1 PublicationVSP_032852Add
BLAST
Alternative sequencei257 – 2615VEERR → LPEAQ in isoform 2. 1 PublicationVSP_032853
Alternative sequencei262 – 30039Missing in isoform 2. 1 PublicationVSP_032854Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006972 mRNA. Translation: BAB24814.1. Frameshift.
AK082816 mRNA. Translation: BAC38632.1. Different initiation.
AK148222 mRNA. Translation: BAE28422.1.
AK162770 mRNA. Translation: BAE37053.1. Different initiation.
AK166393 mRNA. Translation: BAE38749.1.
AL590968 Genomic DNA. Translation: CAM23034.1. Sequence problems.
AL590968 Genomic DNA. Translation: CAM23035.1.
AL590968 Genomic DNA. Translation: CAM23036.1. Sequence problems.
BC015307 mRNA. Translation: AAH15307.1. Different initiation.
CCDSiCCDS48930.1. [Q3UFY7-2]
CCDS48931.1. [Q3UFY7-1]
RefSeqiNP_001096120.1. NM_001102650.1. [Q3UFY7-1]
NP_080837.3. NM_026561.4. [Q3UFY7-2]
XP_006534106.1. XM_006534043.2. [Q3UFY7-2]
XP_011247499.1. XM_011249197.1. [Q3UFY7-1]
UniGeneiMm.28738.

Genome annotation databases

EnsembliENSMUST00000092688; ENSMUSP00000090360; ENSMUSG00000017176. [Q3UFY7-1]
ENSMUST00000107397; ENSMUSP00000103020; ENSMUSG00000017176. [Q3UFY7-4]
ENSMUST00000107398; ENSMUSP00000103021; ENSMUSG00000017176. [Q3UFY7-2]
ENSMUST00000107399; ENSMUSP00000103022; ENSMUSG00000017176. [Q3UFY7-2]
GeneIDi68106.
KEGGimmu:68106.
UCSCiuc007llc.1. mouse. [Q3UFY7-2]
uc007lld.2. mouse. [Q3UFY7-1]
uc007llg.1. mouse. [Q3UFY7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006972 mRNA. Translation: BAB24814.1. Frameshift.
AK082816 mRNA. Translation: BAC38632.1. Different initiation.
AK148222 mRNA. Translation: BAE28422.1.
AK162770 mRNA. Translation: BAE37053.1. Different initiation.
AK166393 mRNA. Translation: BAE38749.1.
AL590968 Genomic DNA. Translation: CAM23034.1. Sequence problems.
AL590968 Genomic DNA. Translation: CAM23035.1.
AL590968 Genomic DNA. Translation: CAM23036.1. Sequence problems.
BC015307 mRNA. Translation: AAH15307.1. Different initiation.
CCDSiCCDS48930.1. [Q3UFY7-2]
CCDS48931.1. [Q3UFY7-1]
RefSeqiNP_001096120.1. NM_001102650.1. [Q3UFY7-1]
NP_080837.3. NM_026561.4. [Q3UFY7-2]
XP_006534106.1. XM_006534043.2. [Q3UFY7-2]
XP_011247499.1. XM_011249197.1. [Q3UFY7-1]
UniGeneiMm.28738.

3D structure databases

ProteinModelPortaliQ3UFY7.
SMRiQ3UFY7. Positions 18-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3UFY7. 1 interaction.
MINTiMINT-4121322.
STRINGi10090.ENSMUSP00000090360.

PTM databases

PhosphoSiteiQ3UFY7.

Proteomic databases

EPDiQ3UFY7.
MaxQBiQ3UFY7.
PaxDbiQ3UFY7.
PRIDEiQ3UFY7.

Protocols and materials databases

DNASUi68106.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092688; ENSMUSP00000090360; ENSMUSG00000017176. [Q3UFY7-1]
ENSMUST00000107397; ENSMUSP00000103020; ENSMUSG00000017176. [Q3UFY7-4]
ENSMUST00000107398; ENSMUSP00000103021; ENSMUSG00000017176. [Q3UFY7-2]
ENSMUST00000107399; ENSMUSP00000103022; ENSMUSG00000017176. [Q3UFY7-2]
GeneIDi68106.
KEGGimmu:68106.
UCSCiuc007llc.1. mouse. [Q3UFY7-2]
uc007lld.2. mouse. [Q3UFY7-1]
uc007llg.1. mouse. [Q3UFY7-3]

Organism-specific databases

CTDi115024.
MGIiMGI:1915356. Nt5c3b.

Phylogenomic databases

eggNOGiKOG3128. Eukaryota.
ENOG410ZQJ8. LUCA.
GeneTreeiENSGT00390000012959.
HOVERGENiHBG059750.
InParanoidiQ3UFY7.
KOiK01081.
OMAiIISEECR.
OrthoDBiEOG7XSTHV.
TreeFamiTF314663.

Enzyme and pathway databases

ReactomeiR-MMU-429958. mRNA decay by 3' to 5' exoribonuclease.

Miscellaneous databases

PROiQ3UFY7.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UFY7.
GenevisibleiQ3UFY7. MM.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
InterProiIPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view]
PfamiPF05822. UMPH-1. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01544. HAD-SF-IE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
    Strain: C57BL/6J.
    Tissue: Testis and Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-300 (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Kidney.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry namei5NT3B_MOUSE
AccessioniPrimary (citable) accession number: Q3UFY7
Secondary accession number(s): A2A4I2
, A2A4I3, Q3TLP3, Q8BHU9, Q91WE8, Q9D9F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: April 3, 2013
Last modified: June 8, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.