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Protein

GRB2-associated and regulator of MAPK protein

Gene

Garem1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an adapter protein that plays a role in intracellular signaling cascades triggered either by the cell surface activated epidermal growth factor receptor and/or cytoplasmic protein tyrosine kinases. Promotes activation of the MAPK/ERK signaling pathway. Plays a role in the regulation of cell proliferation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMitogen

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-associated and regulator of MAPK protein
Alternative name(s):
GRB2-associated and regulator of MAPK1
Gene namesi
Name:Garem1
Synonyms:Fam59a, Garem, Gm944, Kiaa4238
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2685790. Garem1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002776481 – 876GRB2-associated and regulator of MAPK proteinAdd BLAST876

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei105PhosphotyrosineBy similarity1
Modified residuei453PhosphotyrosineBy similarity1
Modified residuei609PhosphoserineCombined sources1
Modified residuei613PhosphoserineCombined sources1

Post-translational modificationi

On EGF stimulation, phosphorylated on Tyr-105 and Tyr-453.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UFT3.
PaxDbiQ3UFT3.
PRIDEiQ3UFT3.

PTM databases

iPTMnetiQ3UFT3.
PhosphoSitePlusiQ3UFT3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042680.
CleanExiMM_GM944.
GenevisibleiQ3UFT3. MM.

Interactioni

Subunit structurei

Interacts with EGFR. Interacts (via proline-rich domain and phosphorylated at Tyr-105 and Tyr-453) with GRB2 (via SH3 domains); the interaction occurs upon EGF stimulation. Interacts (phosphorylated at Tyr-453) with PTPN11; the interaction increases MAPK/ERK activity and does not affect the GRB2/SOS complex formation (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ3UFT3. 1 interactor.
STRINGi10090.ENSMUSP00000048914.

Structurei

3D structure databases

ProteinModelPortaliQ3UFT3.
SMRiQ3UFT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini811 – 876SAMAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 320CABITAdd BLAST309
Regioni498 – 550Necessary for interaction with GRB2By similarityAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi491 – 566Pro-richAdd BLAST76

Sequence similaritiesi

Belongs to the GAREM family.Curated

Phylogenomic databases

eggNOGiENOG410II0U. Eukaryota.
ENOG410XSPW. LUCA.
GeneTreeiENSGT00530000063834.
HOGENOMiHOG000074085.
HOVERGENiHBG060860.
InParanoidiQ3UFT3.
OMAiCQEQFDI.
OrthoDBiEOG091G0K06.
TreeFamiTF329726.

Family and domain databases

InterProiView protein in InterPro
IPR025946. CABIT_dom.
IPR013761. SAM/pointed_sf.
PfamiView protein in Pfam
PF12736. CABIT. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UFT3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPAPSLGCS LKDVKWSPVA MPLDLLVSTY RLPQIARLDS GECVEGLREN
60 70 80 90 100
DFLLIHSCRQ WTTITAHSLE EGHYVIGPKI EIPVHYAGQF KLLEQDRDIK
110 120 130 140 150
EPVQYFNSVE EVAKAFPERV YVMEEITFNV KVASGECNED TEVYNITLCT
160 170 180 190 200
GDELTLMGQA EILYAKTFKE KSRLNTIFKK IGKLNSISKL GKGKMPCLIC
210 220 230 240 250
MNHRTNESIS LPFQCKGRFS TRSPLELQMQ EGEHTIRNIV EKTRLPVNVT
260 270 280 290 300
VPSPPPRNPY DLHFIREGHR YKFVNIQTKT VVVCCVLRNN KILPMHFPLH
310 320 330 340 350
LTVPKFSLPE HQVKGDMWPE TLVHHWLGIC QEQFDIDEYS RAVRDVKTDW
360 370 380 390 400
NEDCKSPKKG RCSGHNHLPN SLSYARDELT QSFHRLSVCV YGNNLHGNSE
410 420 430 440 450
VNLHGCRDLG GEWAPFPHDI LPYQDSGDSG SDYLFPEANE ESAGIPGKTE
460 470 480 490 500
VPYEELWLEE GKPSRQPLTR SLSEKSRCDT LRGSTRSTCA PSSPPTPATL
510 520 530 540 550
GATIKSSEIA LPPPPVPPKS EAVREECRLL NAPPVPPRSA KPLSTSPSIP
560 570 580 590 600
PRTVKPVRPQ TRSPSPTLSY YSSGLHNIIT QSDTSPPNSA PVSCYPCTRV
610 620 630 640 650
KSDSVDPKSP FGSPSAEALS SRLSWPNHYS GASENQTRSD FLLDPSRSYS
660 670 680 690 700
YPRQKTPGTP KRTCPAPFDF EGCELLGSPP STTSAEFSSS GVPSCPKSAS
710 720 730 740 750
YCLENSEDNS FAAGMTKQSV SCPALPPRAP KPVEQKATPE TSPLPLKIDG
760 770 780 790 800
AEEDPTAGSL DLSEDQYFVR KGMQDIFSVS YPFSSPLHLQ LAPRSCGDGS
810 820 830 840 850
PWQPPADLSG LSIEEVSKSL RFIGLSEDVI AFFVTEKIDG NLLVQLTEEI
860 870
LSEDFKLSKL QVKKILQFIN GWRPKI
Length:876
Mass (Da):97,250
Last modified:October 3, 2012 - v2
Checksum:i8CC941A2A072DE0A
GO
Isoform 2 (identifier: Q3UFT3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     578-578: I → M
     579-876: Missing.

Show »
Length:578
Mass (Da):64,994
Checksum:i26CF9712841A40CB
GO

Sequence cautioni

The sequence BAD90542 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti324H → N in BAE28477 (PubMed:16141072).Curated1
Sequence conflicti652P → H in BAE28477 (PubMed:16141072).Curated1
Sequence conflicti857L → Q in BAE36773 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023049578I → M in isoform 2. 1 Publication1
Alternative sequenceiVSP_023050579 – 876Missing in isoform 2. 1 PublicationAdd BLAST298

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK148318 mRNA. Translation: BAE28477.1.
AK162177 mRNA. Translation: BAE36773.1.
AK220564 mRNA. Translation: BAD90542.1. Different initiation.
AC127570 Genomic DNA. No translation available.
AC158774 Genomic DNA. No translation available.
CH466557 Genomic DNA. Translation: EDK96964.1.
BC049983 mRNA. Translation: AAH49983.1.
CCDSiCCDS29091.1. [Q3UFT3-1]
RefSeqiNP_001028617.2. NM_001033445.2. [Q3UFT3-1]
UniGeneiMm.312276.

Genome annotation databases

EnsembliENSMUST00000049260; ENSMUSP00000048914; ENSMUSG00000042680. [Q3UFT3-1]
GeneIDi381126.
KEGGimmu:381126.
UCSCiuc008efg.1. mouse. [Q3UFT3-1]
uc008efh.1. mouse. [Q3UFT3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGARE1_MOUSE
AccessioniPrimary (citable) accession number: Q3UFT3
Secondary accession number(s): G5E8B7
, Q3TSA2, Q5DTF6, Q80V96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: October 3, 2012
Last modified: November 22, 2017
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families