Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

cAMP-specific 3',5'-cyclic phosphodiesterase 4C

Gene

Pde4c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by rolipram.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei389 – 3891Proton donorBy similarity
Metal bindingi393 – 3931Divalent metal cation 1By similarity
Metal bindingi429 – 4291Divalent metal cation 1By similarity
Metal bindingi430 – 4301Divalent metal cation 1By similarity
Metal bindingi430 – 4301Divalent metal cation 2By similarity
Binding sitei430 – 4301cAMPBy similarity
Metal bindingi547 – 5471Divalent metal cation 1By similarity
Binding sitei547 – 5471cAMPBy similarity
Sitei550 – 5501Binds AMP, but not cAMPBy similarity
Binding sitei598 – 5981cAMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi389 – 3935cAMPBy similarity

GO - Molecular functioni

  1. 3',5'-cyclic-nucleotide phosphodiesterase activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP catabolic process Source: UniProtKB-UniPathway
  2. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_203795. DARPP-32 events.
REACT_242871. G alpha (s) signalling events.
UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase 4C (EC:3.1.4.53)
Gene namesi
Name:Pde4c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:99556. Pde4c.

Subcellular locationi

Cell projectioncilium 1 Publication

GO - Cellular componenti

  1. extracellular space Source: MGI
  2. primary cilium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686cAMP-specific 3',5'-cyclic phosphodiesterase 4CPRO_0000198812Add
BLAST

Proteomic databases

MaxQBiQ3UEI1.
PaxDbiQ3UEI1.
PRIDEiQ3UEI1.

PTM databases

PhosphoSiteiQ3UEI1.

Expressioni

Gene expression databases

BgeeiQ3UEI1.
CleanExiMM_PDE4C.
ExpressionAtlasiQ3UEI1. baseline and differential.
GenevestigatoriQ3UEI1.

Interactioni

Subunit structurei

Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2.

Protein-protein interaction databases

IntActiQ3UEI1. 1 interaction.
STRINGi10090.ENSMUSP00000105722.

Structurei

3D structure databases

ProteinModelPortaliQ3UEI1.
SMRiQ3UEI1. Positions 317-640.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi659 – 66810Poly-Glu

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG122287.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000236297.
HOVERGENiHBG108239.
InParanoidiQ3UEI1.
KOiK13293.
OMAiLQAENCN.
OrthoDBiEOG7HQNBC.
PhylomeDBiQ3UEI1.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UEI1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRSGTALSF LWTERVREPV DSGVAPVSPL GGGVILRRFS GTLLLPPLSS
60 70 80 90 100
RLGSSGEAES AAHVVFTIGT QGTQRNLGSA QSSFDLENGL PGGKGLLDAQ
110 120 130 140 150
SGPSLGRALQ PPVHHVQRRE SFLYRSDSDH EPSPKAVSRT SSAASDLHGE
160 170 180 190 200
DMIVTPFAQV LASLRTVRNN VAALAHGPGS ATRQVLLGTP PHSSQQAAPT
210 220 230 240 250
EDSGLQLVQE TLEELDWCLE QLETLQTRRS VGEMASNKFK RMLNRELSYL
260 270 280 290 300
SETSRSGNQV SEYISQTFLD QQAEVELPQP PTEDDPWPMA QITELRRSSH
310 320 330 340 350
TSLPTAAIPR FGVQTDQEEQ LAKELEDTNK WGLDVFKVAE LSGNRPLTAV
360 370 380 390 400
IFSVFQERDL LKTFQIPADT LLAYLLTLEG HYHSDVAYHN SMHAADVVQS
410 420 430 440 450
AHVLLGTPAL EAVFTDLEVL AAIFACAIHD VDHPGVSNQF LINTNSELAL
460 470 480 490 500
MYNDSSVLEN HHLAVGFKLL QGENCDIFRN LSTKQRLSLR RMVIDMVLAT
510 520 530 540 550
DMSKHMSLLA DLKTMVETKK VTSLGVLLLD NYSDRIQVLQ SLVHCADLSN
560 570 580 590 600
PAKPLPLYRQ WTERIMAEFF QQGDRERESG LDISPMCDKH TASMEKSQVG
610 620 630 640 650
FIDYIAQPLW ETWADLVHPD AQELLDTLED NREWYQSRIP CSPPHTMGSD
660 670 680
RFKFELTLEE AEEEEEEEDE GQCTALNRES SELPST
Length:686
Mass (Da):76,090
Last modified:October 11, 2005 - v1
Checksum:i4B510001564A2A08
GO
Isoform 2 (identifier: Q3UEI1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     537-570: Missing.

Note: No experimental confirmation available.

Show »
Length:652
Mass (Da):72,106
Checksum:i75B22BC077830AA9
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei537 – 57034Missing in isoform 2. 1 PublicationVSP_016663Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149514 mRNA. Translation: BAE28930.1.
BC030873 mRNA. Translation: AAH30873.1.
CCDSiCCDS22378.1. [Q3UEI1-2]
RefSeqiNP_963901.1. NM_201607.2. [Q3UEI1-2]
UniGeneiMm.255180.

Genome annotation databases

EnsembliENSMUST00000034307; ENSMUSP00000034307; ENSMUSG00000031842. [Q3UEI1-2]
ENSMUST00000110095; ENSMUSP00000105722; ENSMUSG00000031842. [Q3UEI1-1]
GeneIDi110385.
KEGGimmu:110385.
UCSCiuc009mbg.1. mouse. [Q3UEI1-2]
uc009mbh.1. mouse. [Q3UEI1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149514 mRNA. Translation: BAE28930.1.
BC030873 mRNA. Translation: AAH30873.1.
CCDSiCCDS22378.1. [Q3UEI1-2]
RefSeqiNP_963901.1. NM_201607.2. [Q3UEI1-2]
UniGeneiMm.255180.

3D structure databases

ProteinModelPortaliQ3UEI1.
SMRiQ3UEI1. Positions 317-640.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3UEI1. 1 interaction.
STRINGi10090.ENSMUSP00000105722.

Chemistry

ChEMBLiCHEMBL2111373.

PTM databases

PhosphoSiteiQ3UEI1.

Proteomic databases

MaxQBiQ3UEI1.
PaxDbiQ3UEI1.
PRIDEiQ3UEI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034307; ENSMUSP00000034307; ENSMUSG00000031842. [Q3UEI1-2]
ENSMUST00000110095; ENSMUSP00000105722; ENSMUSG00000031842. [Q3UEI1-1]
GeneIDi110385.
KEGGimmu:110385.
UCSCiuc009mbg.1. mouse. [Q3UEI1-2]
uc009mbh.1. mouse. [Q3UEI1-1]

Organism-specific databases

CTDi5143.
MGIiMGI:99556. Pde4c.

Phylogenomic databases

eggNOGiNOG122287.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000236297.
HOVERGENiHBG108239.
InParanoidiQ3UEI1.
KOiK13293.
OMAiLQAENCN.
OrthoDBiEOG7HQNBC.
PhylomeDBiQ3UEI1.
TreeFamiTF314638.

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.
ReactomeiREACT_203795. DARPP-32 events.
REACT_242871. G alpha (s) signalling events.

Miscellaneous databases

ChiTaRSiPde4c. mouse.
NextBioi363905.
PROiQ3UEI1.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UEI1.
CleanExiMM_PDE4C.
ExpressionAtlasiQ3UEI1. baseline and differential.
GenevestigatoriQ3UEI1.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Kidney.
  3. "Polycystin-2 and phosphodiesterase 4C are components of a ciliary A-kinase anchoring protein complex that is disrupted in cystic kidney diseases."
    Choi Y.H., Suzuki A., Hajarnis S., Ma Z., Chapin H.C., Caplan M.J., Pontoglio M., Somlo S., Igarashi P.
    Proc. Natl. Acad. Sci. U.S.A. 108:10679-10684(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH AKAP5; ADCY5; ADCY6 AND PKD2.

Entry informationi

Entry nameiPDE4C_MOUSE
AccessioniPrimary (citable) accession number: Q3UEI1
Secondary accession number(s): Q8K0P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2005
Last modified: February 4, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.