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Protein

cAMP-specific 3',5'-cyclic phosphodiesterase 4C

Gene

Pde4c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by rolipram.By similarity

Pathwayi: 3',5'-cyclic AMP degradation

This protein is involved in step 1 of the subpathway that synthesizes AMP from 3',5'-cyclic AMP.
Proteins known to be involved in this subpathway in this organism are:
  1. High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B (Pde8b), cAMP-specific 3',5'-cyclic phosphodiesterase 4A (Pde4a), cAMP-specific 3',5'-cyclic phosphodiesterase 4C (Pde4c), High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A (Pde8a), cAMP-specific 3',5'-cyclic phosphodiesterase 4D (Pde4d), High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A (Pde7a), cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A (Pde10a), cAMP-specific 3',5'-cyclic phosphodiesterase 7B (Pde7b)
This subpathway is part of the pathway 3',5'-cyclic AMP degradation, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from 3',5'-cyclic AMP, the pathway 3',5'-cyclic AMP degradation and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei389Proton donorBy similarity1
Metal bindingi393Divalent metal cation 1By similarity1
Metal bindingi429Divalent metal cation 1By similarity1
Metal bindingi430Divalent metal cation 1By similarity1
Metal bindingi430Divalent metal cation 2By similarity1
Binding sitei430cAMPBy similarity1
Metal bindingi547Divalent metal cation 1By similarity1
Binding sitei547cAMPBy similarity1
Sitei550Binds AMP, but not cAMPBy similarity1
Binding sitei598cAMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi389 – 393cAMPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-180024. DARPP-32 events.
R-MMU-418555. G alpha (s) signalling events.
UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase 4C (EC:3.1.4.53)
Gene namesi
Name:Pde4c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:99556. Pde4c.

Subcellular locationi

GO - Cellular componenti

  • cilium Source: UniProtKB
  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2111373.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988121 – 686cAMP-specific 3',5'-cyclic phosphodiesterase 4CAdd BLAST686

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9PhosphoserineCombined sources1
Modified residuei28PhosphoserineCombined sources1
Modified residuei40PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei642PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UEI1.
PaxDbiQ3UEI1.
PRIDEiQ3UEI1.

PTM databases

iPTMnetiQ3UEI1.
PhosphoSitePlusiQ3UEI1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031842.
CleanExiMM_PDE4C.
ExpressionAtlasiQ3UEI1. baseline and differential.

Interactioni

Subunit structurei

Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2.

Protein-protein interaction databases

IntActiQ3UEI1. 1 interactor.
STRINGi10090.ENSMUSP00000034307.

Structurei

3D structure databases

ProteinModelPortaliQ3UEI1.
SMRiQ3UEI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi659 – 668Poly-Glu10

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3689. Eukaryota.
ENOG410XRI7. LUCA.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000236297.
HOVERGENiHBG108239.
InParanoidiQ3UEI1.
KOiK13293.
OMAiEDTNRWG.
OrthoDBiEOG091G06CD.
PhylomeDBiQ3UEI1.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UEI1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRSGTALSF LWTERVREPV DSGVAPVSPL GGGVILRRFS GTLLLPPLSS
60 70 80 90 100
RLGSSGEAES AAHVVFTIGT QGTQRNLGSA QSSFDLENGL PGGKGLLDAQ
110 120 130 140 150
SGPSLGRALQ PPVHHVQRRE SFLYRSDSDH EPSPKAVSRT SSAASDLHGE
160 170 180 190 200
DMIVTPFAQV LASLRTVRNN VAALAHGPGS ATRQVLLGTP PHSSQQAAPT
210 220 230 240 250
EDSGLQLVQE TLEELDWCLE QLETLQTRRS VGEMASNKFK RMLNRELSYL
260 270 280 290 300
SETSRSGNQV SEYISQTFLD QQAEVELPQP PTEDDPWPMA QITELRRSSH
310 320 330 340 350
TSLPTAAIPR FGVQTDQEEQ LAKELEDTNK WGLDVFKVAE LSGNRPLTAV
360 370 380 390 400
IFSVFQERDL LKTFQIPADT LLAYLLTLEG HYHSDVAYHN SMHAADVVQS
410 420 430 440 450
AHVLLGTPAL EAVFTDLEVL AAIFACAIHD VDHPGVSNQF LINTNSELAL
460 470 480 490 500
MYNDSSVLEN HHLAVGFKLL QGENCDIFRN LSTKQRLSLR RMVIDMVLAT
510 520 530 540 550
DMSKHMSLLA DLKTMVETKK VTSLGVLLLD NYSDRIQVLQ SLVHCADLSN
560 570 580 590 600
PAKPLPLYRQ WTERIMAEFF QQGDRERESG LDISPMCDKH TASMEKSQVG
610 620 630 640 650
FIDYIAQPLW ETWADLVHPD AQELLDTLED NREWYQSRIP CSPPHTMGSD
660 670 680
RFKFELTLEE AEEEEEEEDE GQCTALNRES SELPST
Length:686
Mass (Da):76,090
Last modified:October 11, 2005 - v1
Checksum:i4B510001564A2A08
GO
Isoform 2 (identifier: Q3UEI1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     537-570: Missing.

Note: No experimental confirmation available.
Show »
Length:652
Mass (Da):72,106
Checksum:i75B22BC077830AA9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016663537 – 570Missing in isoform 2. 1 PublicationAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149514 mRNA. Translation: BAE28930.1.
BC030873 mRNA. Translation: AAH30873.1.
CCDSiCCDS22378.1. [Q3UEI1-2]
CCDS80891.1. [Q3UEI1-1]
RefSeqiNP_001297394.1. NM_001310465.1. [Q3UEI1-1]
NP_963901.1. NM_201607.2. [Q3UEI1-2]
UniGeneiMm.255180.

Genome annotation databases

EnsembliENSMUST00000034307; ENSMUSP00000034307; ENSMUSG00000031842. [Q3UEI1-2]
ENSMUST00000110095; ENSMUSP00000105722; ENSMUSG00000031842. [Q3UEI1-1]
GeneIDi110385.
KEGGimmu:110385.
UCSCiuc009mbg.1. mouse. [Q3UEI1-2]
uc009mbh.1. mouse. [Q3UEI1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149514 mRNA. Translation: BAE28930.1.
BC030873 mRNA. Translation: AAH30873.1.
CCDSiCCDS22378.1. [Q3UEI1-2]
CCDS80891.1. [Q3UEI1-1]
RefSeqiNP_001297394.1. NM_001310465.1. [Q3UEI1-1]
NP_963901.1. NM_201607.2. [Q3UEI1-2]
UniGeneiMm.255180.

3D structure databases

ProteinModelPortaliQ3UEI1.
SMRiQ3UEI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3UEI1. 1 interactor.
STRINGi10090.ENSMUSP00000034307.

Chemistry databases

ChEMBLiCHEMBL2111373.

PTM databases

iPTMnetiQ3UEI1.
PhosphoSitePlusiQ3UEI1.

Proteomic databases

MaxQBiQ3UEI1.
PaxDbiQ3UEI1.
PRIDEiQ3UEI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034307; ENSMUSP00000034307; ENSMUSG00000031842. [Q3UEI1-2]
ENSMUST00000110095; ENSMUSP00000105722; ENSMUSG00000031842. [Q3UEI1-1]
GeneIDi110385.
KEGGimmu:110385.
UCSCiuc009mbg.1. mouse. [Q3UEI1-2]
uc009mbh.1. mouse. [Q3UEI1-1]

Organism-specific databases

CTDi5143.
MGIiMGI:99556. Pde4c.

Phylogenomic databases

eggNOGiKOG3689. Eukaryota.
ENOG410XRI7. LUCA.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000236297.
HOVERGENiHBG108239.
InParanoidiQ3UEI1.
KOiK13293.
OMAiEDTNRWG.
OrthoDBiEOG091G06CD.
PhylomeDBiQ3UEI1.
TreeFamiTF314638.

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.
ReactomeiR-MMU-180024. DARPP-32 events.
R-MMU-418555. G alpha (s) signalling events.

Miscellaneous databases

ChiTaRSiPde4c. mouse.
PROiQ3UEI1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031842.
CleanExiMM_PDE4C.
ExpressionAtlasiQ3UEI1. baseline and differential.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDE4C_MOUSE
AccessioniPrimary (citable) accession number: Q3UEI1
Secondary accession number(s): Q8K0P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.