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Protein

Pyruvate kinase

Gene

Pklr

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm7293), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs), Glyceraldehyde-3-phosphate dehydrogenase (Gm3839)
  2. Phosphoglycerate kinase 2 (Pgk2), Phosphoglycerate kinase 1 (Pgk1)
  3. no protein annotated in this organism
  4. Gamma-enolase (Eno2), Beta-enolase (Eno3), Alpha-enolase (Eno1), Enolase 4 (Eno4)
  5. Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKLR (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase PKM (Pkm)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinaseUniRule annotation (EC:2.7.1.40UniRule annotation)
Gene namesi
Name:PklrImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:97604. Pklr.

PTM / Processingi

Proteomic databases

PaxDbiQ3UEH4.
PRIDEiQ3UEH4.

Expressioni

Gene expression databases

GenevisibleiQ3UEH4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035417.

Structurei

3D structure databases

ProteinModelPortaliQ3UEH4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 406352PKInterPro annotationAdd
BLAST
Domaini423 – 540118PK_CInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the pyruvate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOVERGENiHBG000941.
KOiK12406.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UEH4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGPAGYLRR ASVAQLTQEL GTAFFQQQQL PAAMADTFLE HLCLLDIDSE
60 70 80 90 100
PVAARSTSII ATIGPASRSV DRLKEMIKAG MNIARLNFSH GSHEYHAESI
110 120 130 140 150
ANIREAAESF ATSPLSYRPV AIALDTKGPE IRTGVLQGGP ESEVEIVKGS
160 170 180 190 200
QVLVTVDPKF RTRGDAKTVW VDYHNITQVV AVGGRIYIDD GLISLVVRKI
210 220 230 240 250
GPEGLVTEVE HGGFLGNRKG VNLPNAEVDL PGLSEQDLLD LRFGVEHNVD
260 270 280 290 300
IIFASFVRKA SDVVAVRDAL GPEGRGIKII SKIENHEGVK KFDEILEVSD
310 320 330 340 350
GIMVARGDLG IEIPAEKVFL AQKMMIGRCN LAGKPVVCAT QMLESMITKA
360 370 380 390 400
RPTRAETSDV ANAVLDGADC IMLSGETAKG SFPVEAVKMQ HAIVREAEAA
410 420 430 440 450
VYHRQLFEEL RRAAPLSRDP TEVTAIGAVE ASFKCCAAAI IVLTKTGRSA
460 470 480 490 500
QLLSRYRPRA AVIAVTRSAQ AARQVHLSRG VFPLLYREPP EAVWADDVDR
510 520 530 540
RVQFGIESGK LRGFLRVGDL VIVVTGWRPG SGYTNIMRVL TIS
Length:543
Mass (Da):58,849
Last modified:October 11, 2005 - v1
Checksum:iD2965CC5F566E0F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149524 mRNA. Translation: BAE28937.1.
RefSeqiNP_001093249.1. NM_001099779.1.
NP_038659.2. NM_013631.2.
UniGeneiMm.383180.

Genome annotation databases

GeneIDi18770.
KEGGimmu:18770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK149524 mRNA. Translation: BAE28937.1.
RefSeqiNP_001093249.1. NM_001099779.1.
NP_038659.2. NM_013631.2.
UniGeneiMm.383180.

3D structure databases

ProteinModelPortaliQ3UEH4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035417.

Proteomic databases

PaxDbiQ3UEH4.
PRIDEiQ3UEH4.

Protocols and materials databases

DNASUi18770.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18770.
KEGGimmu:18770.

Organism-specific databases

CTDi5313.
MGIiMGI:97604. Pklr.

Phylogenomic databases

eggNOGiKOG2323. Eukaryota.
COG0469. LUCA.
HOVERGENiHBG000941.
KOiK12406.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Miscellaneous databases

ChiTaRSiPklr. mouse.
SOURCEiSearch...

Gene expression databases

GenevisibleiQ3UEH4. MM.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "High-efficiency full-length cDNA cloning."
    Carninci P., Hayashizaki Y.
    Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: LiverImported.
  2. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
    Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
    Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: LiverImported.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: LiverImported.
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: LiverImported.
  5. "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
    The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
    Nature 420:563-573(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: LiverImported.
  6. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: LiverImported.
  7. "The Transcriptional Landscape of the Mammalian Genome."
    The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
    Science 309:1559-1563(2005)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: LiverImported.
  8. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: LiverImported.

Entry informationi

Entry nameiQ3UEH4_MOUSE
AccessioniPrimary (citable) accession number: Q3UEH4
Entry historyi
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: July 6, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.