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Protein

Glutathione synthetase

Gene

Gss

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase catalytic subunit (Gclc), Glutamate--cysteine ligase regulatory subunit (Gclm)
  2. Glutathione synthetase (Gss), Glutathione synthetase (Gss), Glutathione synthetase (Gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561SubstrateUniRule annotation
Metal bindingi75 – 751MagnesiumUniRule annotation
Binding sitei75 – 751ATPUniRule annotation
Metal bindingi77 – 771MagnesiumUniRule annotation
Binding sitei151 – 1511SubstrateUniRule annotation
Binding sitei236 – 2361ATPUniRule annotation
Metal bindingi299 – 2991MagnesiumUniRule annotation
Binding sitei306 – 3061ATPUniRule annotation
Binding sitei356 – 3561ATPUniRule annotation
Binding sitei381 – 3811SubstrateUniRule annotation
Binding sitei383 – 3831ATPUniRule annotation
Binding sitei389 – 3891ATP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi295 – 30410ATPUniRule annotation
Nucleotide bindingi329 – 3324ATPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Glutathione biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
Gene namesi
Name:GssImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:95852. Gss.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027610.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078630.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini136 – 23398GSH_synthaseInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
GeneTreeiENSGT00390000013764.
HOVERGENiHBG002458.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3UEE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFNILVDAV SQNPAFLEQT LSSTIKKDDY TARLFDIYKQ VLKEGIAQTV
60 70 80 90 100
FLGLNRSDYM FQCGADGSKA LKQIEINTIS ASFGGLASRT PAVHRHVLNV
110 120 130 140 150
LNKTKEASKI LSNNPSKGLA LGIAKAWELY GSANAVVLLI AQEKERNIFD
160 170 180 190 200
QRAVENELLD RKIHVIRGRF EDVSERGSLD QNRRLFMDDQ EVAVVYFRDG
210 220 230 240 250
YMPSQYNSQN WEARLMLERS RAAKCPDIAI QLAGTKKVQQ ELSRVGLLEA
260 270 280 290 300
LLPGQPEAVA RLRATFAGLY SLDMGEEGDQ AIAEALAAPS HFVLKPQREG
310 320 330 340 350
GGNNLYGEEM VQALEQLKDS EERASYILME KIEPEPFRNC LLRPGSPAQV
360 370 380 390 400
VQCISELGIF GVYVRQGTTL VMNKHVGHLL RTKAVEHADG GVAAGVAVLD

NPYPV
Length:405
Mass (Da):44,792
Last modified:October 11, 2005 - v1
Checksum:i844C34DE89A8A683
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844852 Genomic DNA. No translation available.
AK149577 mRNA. Translation: BAE28969.1.
RefSeqiNP_001278040.1. NM_001291111.1.
UniGeneiMm.252316.

Genome annotation databases

EnsembliENSMUST00000130881; ENSMUSP00000135319; ENSMUSG00000027610.
GeneIDi14854.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844852 Genomic DNA. No translation available.
AK149577 mRNA. Translation: BAE28969.1.
RefSeqiNP_001278040.1. NM_001291111.1.
UniGeneiMm.252316.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078630.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000130881; ENSMUSP00000135319; ENSMUSG00000027610.
GeneIDi14854.

Organism-specific databases

CTDi2937.
MGIiMGI:95852. Gss.

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
GeneTreeiENSGT00390000013764.
HOVERGENiHBG002458.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027610.

Family and domain databases

Gene3Di1.10.1080.10. 2 hits.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ3UEE2_MOUSE
AccessioniPrimary (citable) accession number: Q3UEE2
Entry historyi
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.