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Protein

TRAF-type zinc finger domain-containing protein 1

Gene

Trafd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negative feedback regulator that controls excessive innate immune responses. Regulates both Toll-like receptor 4 (TLR4) and DDX58/RIG1-like helicases (RLH) pathways. May inhibit the LTR pathway by direct interaction with TRAF6 and attenuation of NF-kappa-B activation. May negatively regulate the RLH pathway downstream from MAVS and upstream of NF-kappa-B and IRF3.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 103TRAF-typePROSITE-ProRule annotationAdd BLAST77

GO - Molecular functioni

GO - Biological processi

  • negative regulation of innate immune response Source: UniProtKB
  • response to cytokine Source: GO_Central

Keywordsi

LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
TRAF-type zinc finger domain-containing protein 1
Alternative name(s):
Protein FLN29
Gene namesi
Name:Trafd1
Synonyms:Fln29
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1923551 Trafd1

Pathology & Biotechi

Disruption phenotypei

Mice show no gross developmental abnormalities, but exhibit an increased susceptibility to LPS-induced septic shock and are more sensitive to poly(I:C) shock, suffering more severe hepatic damage than wild-type animals. In response to LPS-stimulation, bone marrow-derived dendritic cells display an increased production of pro-inflammatory cytokines, such as IL6, TNF and IL12, as well as elevated IKK and JNK activation, compared to wild-type mice. Mutant embryonic fibroblasts are more resistant to vesicular stomatitis virus (VSV)-induced cytopathic effect.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi294E → A: No effect on TRAF6-induced NF-kappa-B activation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002784592 – 580TRAF-type zinc finger domain-containing protein 1Add BLAST579

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei278PhosphoserineBy similarity1
Modified residuei320PhosphoserineBy similarity1
Modified residuei326PhosphoserineBy similarity1
Modified residuei327PhosphoserineBy similarity1
Modified residuei409PhosphoserineBy similarity1
Modified residuei415PhosphoserineBy similarity1
Modified residuei430PhosphoserineBy similarity1
Modified residuei450PhosphoserineBy similarity1
Modified residuei469PhosphoserineBy similarity1
Modified residuei532PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ3UDK1
PaxDbiQ3UDK1
PeptideAtlasiQ3UDK1
PRIDEiQ3UDK1

PTM databases

iPTMnetiQ3UDK1
PhosphoSitePlusiQ3UDK1

Expressioni

Tissue specificityi

Expressed in skeletal muscle, brain, liver, kidney, spleen and bone marrow. Expression depends on STAT1.1 Publication

Inductioni

By lipopolysaccharide (LPS), IFNB1 and IFNG, IFNB1 being most rapid and potent inducer (at protein level). Not induced by anti-inflammatory cytokines, such as IL4 and IL10, which also inhibit LPS induction of TRAFD1.1 Publication

Gene expression databases

BgeeiENSMUSG00000042726
CleanExiMM_TRAFD1
ExpressionAtlasiQ3UDK1 baseline and differential
GenevisibleiQ3UDK1 MM

Interactioni

Subunit structurei

Interacts with MAVS, TICAM1, TRAF1, TRAF2, TRAF3 and TRAF6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Traf6P701962EBI-1396948,EBI-448028

Protein-protein interaction databases

BioGridi231158, 1 interactor
IntActiQ3UDK1, 2 interactors
STRINGi10090.ENSMUSP00000047475

Structurei

3D structure databases

ProteinModelPortaliQ3UDK1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi576 – 580Poly-Glu5

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri27 – 103TRAF-typePROSITE-ProRule annotationAdd BLAST77

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGBJ Eukaryota
ENOG4111IF3 LUCA
GeneTreeiENSGT00530000063869
HOGENOMiHOG000154674
HOVERGENiHBG094084
InParanoidiQ3UDK1
OMAiAEQDFWR
OrthoDBiEOG091G0OSL
PhylomeDBiQ3UDK1
TreeFamiTF331416

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013083 Znf_RING/FYVE/PHD
IPR001293 Znf_TRAF
PROSITEiView protein in PROSITE
PS50145 ZF_TRAF, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UDK1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEFRDDQAS RLCDNCKKEI PVFNFTIHEI HCQRNIGVCP VCKEPFPKSD
60 70 80 90 100
MDIHMAAEHC QVTCKCNKKL EKRQLKQHAE TECPLRLAVC QHCDLELSVV
110 120 130 140 150
KLKEHEDYCG ARTELCGSCG RNVLVKELKT HPEVCGRVEE EKRTEAAIPP
160 170 180 190 200
EAYDEPWSQD RIWIASQLLR QIEALDPPMR LPGRPLQAFE ADPFYSRTTS
210 220 230 240 250
QRSMAAQFPV QNNLFEEQER QERNRSRQSP KDSAENNAHL DFMLALSLQN
260 270 280 290 300
EGQATSMVEQ GFWESVPEAD PARAGPTSLG DIKGAADEIL LPCEFCEELY
310 320 330 340 350
PEELLIDHQT SCNPSHALRS LNTGSSSIRG VEDPGTIFQN FLQQATSNQF
360 370 380 390 400
DTLMGLSSSA AVEDSIIIPC EFCGVQLEEE VLFYHQDQCD QRPATANHRA
410 420 430 440 450
VEGIPAQDSQ PENTSAELSR RRVKHQGDLS SGYMDDVKPE SVKGPTYSMS
460 470 480 490 500
PNRTMNNVAS CNRLLNLPSG PRSDCQRSPP GVLKLNNSDS QDIRGQMRGS
510 520 530 540 550
QNGPIASGHA PVIHSIQNLY PENFAPSFPH GSPGRYGAGG RSEGGRSSRV
560 570 580
SPAAAGYHSR AAKAKPPKQQ GAGDAEEEEE
Length:580
Mass (Da):64,283
Last modified:October 11, 2005 - v1
Checksum:iADDABA43305FB70B
GO
Isoform 2 (identifier: Q3UDK1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     535-539: RYGAG → S

Note: No experimental confirmation available.
Show »
Length:576
Mass (Da):63,865
Checksum:iE3A10362C1D6915C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti328I → V in AAH38396 (PubMed:15489334).Curated1
Sequence conflicti331V → M in AAH38396 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023294535 – 539RYGAG → S in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150038 mRNA Translation: BAE29260.1
BC038396 mRNA Translation: AAH38396.1
CCDSiCCDS51638.1 [Q3UDK1-1]
CCDS51639.1 [Q3UDK1-2]
RefSeqiNP_001156942.1, NM_001163470.1 [Q3UDK1-1]
NP_758479.2, NM_172275.2 [Q3UDK1-2]
XP_006530365.1, XM_006530302.3 [Q3UDK1-1]
XP_011246499.1, XM_011248197.2 [Q3UDK1-1]
XP_017176353.1, XM_017320864.1 [Q3UDK1-1]
XP_017176354.1, XM_017320865.1 [Q3UDK1-2]
XP_017176355.1, XM_017320866.1 [Q3UDK1-2]
UniGeneiMm.20488

Genome annotation databases

EnsembliENSMUST00000042312; ENSMUSP00000047475; ENSMUSG00000042726 [Q3UDK1-1]
ENSMUST00000120784; ENSMUSP00000113910; ENSMUSG00000042726 [Q3UDK1-2]
GeneIDi231712
KEGGimmu:231712
UCSCiuc008ziy.2 mouse [Q3UDK1-2]
uc008ziz.2 mouse [Q3UDK1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTRAD1_MOUSE
AccessioniPrimary (citable) accession number: Q3UDK1
Secondary accession number(s): Q8CFI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2005
Last modified: April 25, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health