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Protein

TRAF-type zinc finger domain-containing protein 1

Gene

Trafd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Negative feedback regulator that controls excessive innate immune responses. Regulates both Toll-like receptor 4 (TLR4) and DDX58/RIG1-like helicases (RLH) pathways. May inhibit the LTR pathway by direct interaction with TRAF6 and attenuation of NF-kappa-B activation. May negatively regulate the RLH pathway downstream from MAVS and upstream of NF-kappa-B and IRF3.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri27 – 10377TRAF-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • negative regulation of innate immune response Source: UniProtKB
  • response to cytokine Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
TRAF-type zinc finger domain-containing protein 1
Alternative name(s):
Protein FLN29
Gene namesi
Name:Trafd1
Synonyms:Fln29
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1923551. Trafd1.

Pathology & Biotechi

Disruption phenotypei

Mice show no gross developmental abnormalities, but exhibit an increased susceptibility to LPS-induced septic shock and are more sensitive to poly(I:C) shock, suffering more severe hepatic damage than wild-type animals. In response to LPS-stimulation, bone marrow-derived dendritic cells display an increased production of pro-inflammatory cytokines, such as IL6, TNF and IL12, as well as elevated IKK and JNK activation, compared to wild-type mice. Mutant embryonic fibroblasts are more resistant to vesicular stomatitis virus (VSV)-induced cytopathic effect.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi294 – 2941E → A: No effect on TRAF6-induced NF-kappa-B activation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 580579TRAF-type zinc finger domain-containing protein 1PRO_0000278459Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei278 – 2781PhosphoserineBy similarity
Modified residuei320 – 3201PhosphoserineBy similarity
Modified residuei326 – 3261PhosphoserineBy similarity
Modified residuei327 – 3271PhosphoserineBy similarity
Modified residuei409 – 4091PhosphoserineBy similarity
Modified residuei415 – 4151PhosphoserineBy similarity
Modified residuei430 – 4301PhosphoserineBy similarity
Modified residuei450 – 4501PhosphoserineBy similarity
Modified residuei469 – 4691PhosphoserineBy similarity
Modified residuei532 – 5321PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ3UDK1.
PaxDbiQ3UDK1.
PeptideAtlasiQ3UDK1.
PRIDEiQ3UDK1.

PTM databases

iPTMnetiQ3UDK1.
PhosphoSiteiQ3UDK1.

Expressioni

Tissue specificityi

Expressed in skeletal muscle, brain, liver, kidney, spleen and bone marrow. Expression depends on STAT1.1 Publication

Inductioni

By lipopolysaccharide (LPS), IFNB1 and IFNG, IFNB1 being most rapid and potent inducer (at protein level). Not induced by anti-inflammatory cytokines, such as IL4 and IL10, which also inhibit LPS induction of TRAFD1.1 Publication

Gene expression databases

BgeeiENSMUSG00000042726.
CleanExiMM_TRAFD1.
ExpressionAtlasiQ3UDK1. baseline and differential.
GenevisibleiQ3UDK1. MM.

Interactioni

Subunit structurei

Interacts with MAVS, TICAM1, TRAF1, TRAF2, TRAF3 and TRAF6.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Traf6P701962EBI-1396948,EBI-448028

Protein-protein interaction databases

BioGridi231158. 1 interaction.
IntActiQ3UDK1. 2 interactions.
STRINGi10090.ENSMUSP00000047475.

Structurei

3D structure databases

ProteinModelPortaliQ3UDK1.
SMRiQ3UDK1. Positions 6-40, 78-137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi576 – 5805Poly-Glu

Sequence similaritiesi

Contains 1 TRAF-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri27 – 10377TRAF-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IGBJ. Eukaryota.
ENOG4111IF3. LUCA.
GeneTreeiENSGT00530000063869.
HOGENOMiHOG000154674.
HOVERGENiHBG094084.
InParanoidiQ3UDK1.
OMAiAEQDFWR.
OrthoDBiEOG091G0OSL.
PhylomeDBiQ3UDK1.
TreeFamiTF331416.

Family and domain databases

InterProiIPR001293. Znf_TRAF.
[Graphical view]
PROSITEiPS50145. ZF_TRAF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UDK1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEFRDDQAS RLCDNCKKEI PVFNFTIHEI HCQRNIGVCP VCKEPFPKSD
60 70 80 90 100
MDIHMAAEHC QVTCKCNKKL EKRQLKQHAE TECPLRLAVC QHCDLELSVV
110 120 130 140 150
KLKEHEDYCG ARTELCGSCG RNVLVKELKT HPEVCGRVEE EKRTEAAIPP
160 170 180 190 200
EAYDEPWSQD RIWIASQLLR QIEALDPPMR LPGRPLQAFE ADPFYSRTTS
210 220 230 240 250
QRSMAAQFPV QNNLFEEQER QERNRSRQSP KDSAENNAHL DFMLALSLQN
260 270 280 290 300
EGQATSMVEQ GFWESVPEAD PARAGPTSLG DIKGAADEIL LPCEFCEELY
310 320 330 340 350
PEELLIDHQT SCNPSHALRS LNTGSSSIRG VEDPGTIFQN FLQQATSNQF
360 370 380 390 400
DTLMGLSSSA AVEDSIIIPC EFCGVQLEEE VLFYHQDQCD QRPATANHRA
410 420 430 440 450
VEGIPAQDSQ PENTSAELSR RRVKHQGDLS SGYMDDVKPE SVKGPTYSMS
460 470 480 490 500
PNRTMNNVAS CNRLLNLPSG PRSDCQRSPP GVLKLNNSDS QDIRGQMRGS
510 520 530 540 550
QNGPIASGHA PVIHSIQNLY PENFAPSFPH GSPGRYGAGG RSEGGRSSRV
560 570 580
SPAAAGYHSR AAKAKPPKQQ GAGDAEEEEE
Length:580
Mass (Da):64,283
Last modified:October 11, 2005 - v1
Checksum:iADDABA43305FB70B
GO
Isoform 2 (identifier: Q3UDK1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     535-539: RYGAG → S

Note: No experimental confirmation available.
Show »
Length:576
Mass (Da):63,865
Checksum:iE3A10362C1D6915C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti328 – 3281I → V in AAH38396 (PubMed:15489334).Curated
Sequence conflicti331 – 3311V → M in AAH38396 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei535 – 5395RYGAG → S in isoform 2. 1 PublicationVSP_023294

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150038 mRNA. Translation: BAE29260.1.
BC038396 mRNA. Translation: AAH38396.1.
CCDSiCCDS51638.1. [Q3UDK1-1]
CCDS51639.1. [Q3UDK1-2]
RefSeqiNP_001156942.1. NM_001163470.1. [Q3UDK1-1]
NP_758479.2. NM_172275.2. [Q3UDK1-2]
XP_006530365.1. XM_006530302.2. [Q3UDK1-1]
XP_011246499.1. XM_011248197.1. [Q3UDK1-1]
UniGeneiMm.20488.

Genome annotation databases

EnsembliENSMUST00000042312; ENSMUSP00000047475; ENSMUSG00000042726. [Q3UDK1-1]
ENSMUST00000120784; ENSMUSP00000113910; ENSMUSG00000042726. [Q3UDK1-2]
GeneIDi231712.
KEGGimmu:231712.
UCSCiuc008ziy.2. mouse. [Q3UDK1-2]
uc008ziz.2. mouse. [Q3UDK1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150038 mRNA. Translation: BAE29260.1.
BC038396 mRNA. Translation: AAH38396.1.
CCDSiCCDS51638.1. [Q3UDK1-1]
CCDS51639.1. [Q3UDK1-2]
RefSeqiNP_001156942.1. NM_001163470.1. [Q3UDK1-1]
NP_758479.2. NM_172275.2. [Q3UDK1-2]
XP_006530365.1. XM_006530302.2. [Q3UDK1-1]
XP_011246499.1. XM_011248197.1. [Q3UDK1-1]
UniGeneiMm.20488.

3D structure databases

ProteinModelPortaliQ3UDK1.
SMRiQ3UDK1. Positions 6-40, 78-137.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231158. 1 interaction.
IntActiQ3UDK1. 2 interactions.
STRINGi10090.ENSMUSP00000047475.

PTM databases

iPTMnetiQ3UDK1.
PhosphoSiteiQ3UDK1.

Proteomic databases

MaxQBiQ3UDK1.
PaxDbiQ3UDK1.
PeptideAtlasiQ3UDK1.
PRIDEiQ3UDK1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042312; ENSMUSP00000047475; ENSMUSG00000042726. [Q3UDK1-1]
ENSMUST00000120784; ENSMUSP00000113910; ENSMUSG00000042726. [Q3UDK1-2]
GeneIDi231712.
KEGGimmu:231712.
UCSCiuc008ziy.2. mouse. [Q3UDK1-2]
uc008ziz.2. mouse. [Q3UDK1-1]

Organism-specific databases

CTDi10906.
MGIiMGI:1923551. Trafd1.

Phylogenomic databases

eggNOGiENOG410IGBJ. Eukaryota.
ENOG4111IF3. LUCA.
GeneTreeiENSGT00530000063869.
HOGENOMiHOG000154674.
HOVERGENiHBG094084.
InParanoidiQ3UDK1.
OMAiAEQDFWR.
OrthoDBiEOG091G0OSL.
PhylomeDBiQ3UDK1.
TreeFamiTF331416.

Miscellaneous databases

ChiTaRSiTrafd1. mouse.
PROiQ3UDK1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042726.
CleanExiMM_TRAFD1.
ExpressionAtlasiQ3UDK1. baseline and differential.
GenevisibleiQ3UDK1. MM.

Family and domain databases

InterProiIPR001293. Znf_TRAF.
[Graphical view]
PROSITEiPS50145. ZF_TRAF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRAD1_MOUSE
AccessioniPrimary (citable) accession number: Q3UDK1
Secondary accession number(s): Q8CFI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2005
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.