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Protein

Forkhead box protein K2

Gene

Foxk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Positively regulates WNT/beta-catenin signaling by translocating DVL proteins into the nucleus.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi301Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi302Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi304Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi307Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi249 – 344Fork-headPROSITE-ProRule annotationAdd BLAST96

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5689603. UCH proteinases.

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein K2
Alternative name(s):
Cellular transcription factor ILF-1
Interleukin enhancer-binding factor 1
Gene namesi
Name:Foxk2Imported
Synonyms:Ilf11 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1916087. Foxk2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • intracellular membrane-bounded organelle Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002616681 – 651Forkhead box protein K2Add BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Modified residuei135Omega-N-methylarginineBy similarity1
Modified residuei230PhosphoserineBy similarity1
Modified residuei364PhosphoserineCombined sources1
Modified residuei389PhosphoserineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei419PhosphoserineCombined sources1
Cross-linki518Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei590PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ3UCQ1.
PaxDbiQ3UCQ1.
PeptideAtlasiQ3UCQ1.
PRIDEiQ3UCQ1.

PTM databases

iPTMnetiQ3UCQ1.
PhosphoSitePlusiQ3UCQ1.

Expressioni

Tissue specificityi

Expressed in a wide range of adult brain regions, namely the piriform cortex, the major islands of Calleja and cells lining the lateral ventricles, the bed nucleus of stria terminalis, the paraventricular thalamic nucleus, habenula and all structures of the hippocampus. Also present in the hypothalamus, cerebral cortex and in the Purkinje cell layer in the cerebellum. Additionally expressed in dopamine neurons of the substantia and more sparsely in the ventral tegmental area.1 Publication

Developmental stagei

At E12.5, expressed ubiquitously in the developing central nervous system. This pattern persists at E14.5 and E16.5, with expression levels varying.1 Publication

Gene expression databases

BgeeiENSMUSG00000039275.
CleanExiMM_FOXK2.
GenevisibleiQ3UCQ1. MM.

Interactioni

Subunit structurei

Interacts with DVL1, DVL2 (when phosphorylated) and DVL3; the interaction induces DVL2 nuclear translocation. Interacts with SUDS3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101719.

Structurei

3D structure databases

ProteinModelPortaliQ3UCQ1.
SMRiQ3UCQ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 119FHAPROSITE-ProRule annotationAdd BLAST72

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 162Required for interaction with DVL2 and SUDS3By similarityAdd BLAST43
Regioni291 – 309DNA-binding; major grooveBy similarityAdd BLAST19
Regioni319 – 323DNA-binding; minor grooveBy similarity5
Regioni339 – 344DNA-binding; minor grooveBy similarity6

Domaini

The C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity.By similarity

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
GeneTreeiENSGT00800000124014.
HOGENOMiHOG000072588.
HOVERGENiHBG051649.
InParanoidiQ3UCQ1.
KOiK09404.
OMAiKVGRVMP.
OrthoDBiEOG091G099F.
TreeFamiTF325718.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR001766. Fork_head_dom.
IPR008984. SMAD_FHA_domain.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
SM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UCQ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAALSGA GAPPAGGGAG GGGSPPGGWA VARLEGREFE YLMKKRSVTI
60 70 80 90 100
GRNSSQGSVD VSMGHSSFIS RRHLEIFTPP GGGHSAAAPE PAQPRPDAGG
110 120 130 140 150
DFYLRCLGKN GVFVDGVFQR RGAPPLQLPR VCTFRFPSTN IKITFTALSS
160 170 180 190 200
EKREKQEAPE SPVKPVQPHI SPLTINIPDT MAHLISPLPS PTGTISAANS
210 220 230 240 250
CPSSPRGAGS SGYKVGRVMP SDLSLMADNS QPENEKEASG GDSPKDDSKP
260 270 280 290 300
PYSYAQLIVQ AITMAPDKQL TLNGIYTHIT KNYPYYRTAD KGWQNSIRHN
310 320 330 340 350
LSLNRYFIKV PRSQEEPGKG SFWRIDPASE SKLVEQAFRK RRPRGVPCFR
360 370 380 390 400
TPLGPLSSRS APASPNHAGV LSAHSSGAQT PESLSREGSP APLEPEPGAS
410 420 430 440 450
QPKLAVIQEA RFAQSAPGSP LSSQPVLITV QRQLPPAIKP VTYTVATPVT
460 470 480 490 500
TPTSQPPVVQ TVHVVHQIPA VSVTSVAGLA PANTYTVAGQ AVVTQAAVLA
510 520 530 540 550
PPNPEPQENG DHREVRVKVE PVPAISPATL GAASRIIQTS QGTPVQTVTI
560 570 580 590 600
VQQAPLGQHQ LPIKTVTQNG AHVVPMPTAV HSQVNNAAAS PLHMLATHAS
610 620 630 640 650
ASASLPTKRQ NGDQAEQPEL KRVKAEDGES IVIALSVDAP PAAVREKAIQ

N
Note: No experimental confirmation available.Curated
Length:651
Mass (Da):68,446
Last modified:July 27, 2011 - v3
Checksum:iDFD83C2A8EF52EF0
GO
Isoform 21 Publication (identifier: Q3UCQ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     517-586: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:581
Mass (Da):61,211
Checksum:i0CF8418986E7852C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104L → M in BAE29561 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052236517 – 586Missing in isoform 2. 1 PublicationAdd BLAST70

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU882160 mRNA. Translation: ACG63496.1.
AL808021 Genomic DNA. Translation: CAM21743.1.
AK150439 mRNA. Translation: BAE29561.1.
CCDSiCCDS36393.2. [Q3UCQ1-1]
RefSeqiNP_001074401.2. NM_001080932.2. [Q3UCQ1-1]
XP_006534145.1. XM_006534082.1. [Q3UCQ1-2]
UniGeneiMm.209750.

Genome annotation databases

EnsembliENSMUST00000106113; ENSMUSP00000101719; ENSMUSG00000039275. [Q3UCQ1-1]
GeneIDi68837.
KEGGimmu:68837.
UCSCiuc007mvs.1. mouse. [Q3UCQ1-2]
uc011yjl.1. mouse. [Q3UCQ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU882160 mRNA. Translation: ACG63496.1.
AL808021 Genomic DNA. Translation: CAM21743.1.
AK150439 mRNA. Translation: BAE29561.1.
CCDSiCCDS36393.2. [Q3UCQ1-1]
RefSeqiNP_001074401.2. NM_001080932.2. [Q3UCQ1-1]
XP_006534145.1. XM_006534082.1. [Q3UCQ1-2]
UniGeneiMm.209750.

3D structure databases

ProteinModelPortaliQ3UCQ1.
SMRiQ3UCQ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000101719.

PTM databases

iPTMnetiQ3UCQ1.
PhosphoSitePlusiQ3UCQ1.

Proteomic databases

MaxQBiQ3UCQ1.
PaxDbiQ3UCQ1.
PeptideAtlasiQ3UCQ1.
PRIDEiQ3UCQ1.

Protocols and materials databases

DNASUi68837.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106113; ENSMUSP00000101719; ENSMUSG00000039275. [Q3UCQ1-1]
GeneIDi68837.
KEGGimmu:68837.
UCSCiuc007mvs.1. mouse. [Q3UCQ1-2]
uc011yjl.1. mouse. [Q3UCQ1-1]

Organism-specific databases

CTDi3607.
MGIiMGI:1916087. Foxk2.

Phylogenomic databases

eggNOGiKOG2294. Eukaryota.
COG5025. LUCA.
GeneTreeiENSGT00800000124014.
HOGENOMiHOG000072588.
HOVERGENiHBG051649.
InParanoidiQ3UCQ1.
KOiK09404.
OMAiKVGRVMP.
OrthoDBiEOG091G099F.
TreeFamiTF325718.

Enzyme and pathway databases

ReactomeiR-MMU-5689603. UCH proteinases.

Miscellaneous databases

PROiQ3UCQ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039275.
CleanExiMM_FOXK2.
GenevisibleiQ3UCQ1. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR001766. Fork_head_dom.
IPR008984. SMAD_FHA_domain.
IPR018122. TF_fork_head_CS_1.
IPR030456. TF_fork_head_CS_2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF00250. Forkhead. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
SM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOXK2_MOUSE
AccessioniPrimary (citable) accession number: Q3UCQ1
Secondary accession number(s): A2AN27, B5AZX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.