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Protein

DNA ligase

Gene

Lig3

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

DNA damage, DNA recombinationUniRule annotation, DNA repairUniRule annotation, DNA replicationUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-110381. Resolution of AP sites via the single-nucleotide replacement pathway.
R-MMU-5649702. APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway.
R-MMU-5685939. HDR through MMEJ (alt-NHEJ).
R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Gene namesi
Name:Lig3Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:109152. Lig3.

PTM / Processingi

Proteomic databases

PeptideAtlasiQ3UC82.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090525.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 18693PARP-typeInterPro annotationAdd
BLAST
Domaini584 – 718135DNA_LIGASE_A3InterPro annotationAdd
BLAST
Domaini935 – 101177BRCTInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG4437. Eukaryota.
COG1793. LUCA.
GeneTreeiENSGT00840000129855.
HOVERGENiHBG005515.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.30.1740.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR031916. LIG3_BRCT.
IPR012340. NA-bd_OB-fold.
IPR001510. Znf_PARP.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
PF16759. LIG3_BRCT. 1 hit.
PF00645. zf-PARP. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM01336. zf-PARP. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
PS00347. PARP_ZN_FINGER_1. 1 hit.
PS50064. PARP_ZN_FINGER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UC82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLAFKILFP RNLCALGRKE LCLFPEQNRW AAISQFSQWS ETNLLGGCCL
60 70 80 90 100
LQRRKPVLAL QRGHLRPRAT HLTFWPGSHV GLCTGPCAMA EQRFCVDYAK
110 120 130 140 150
RGTAGCKKCK EKIVKGVCRI GKVVPNPFSE SGGDMKEWYH IKCMFEKLER
160 170 180 190 200
ARATTKKIED LTELEGWEEL EDNEKEQISQ HIADLSSKAA ATPKKKAAVQ
210 220 230 240 250
AKLTTTGQVT SPVKGASFIT STNPRKFSGF SAKPNNSEQA PSSPAPGTSL
260 270 280 290 300
SASKCDPKHK DCLLREFRKL CAMVAENPSY NTKTQIIHDF LQKGSTGDGF
310 320 330 340 350
HGDVYLTVKL LLPGVIKSVY NLNDKQIVKL FSRIFNCNPD DMARDLEQGD
360 370 380 390 400
VSETIRIFFE QSKSFPPAAK SLLTIQEVDA FLLHLSKLTK EDEQQQALQD
410 420 430 440 450
IASRCTANDL KCIIRLIKHD LKMNSGAKHV LDALDPNAYE AFKASRNLQD
460 470 480 490 500
VVERVLHNEQ EVEKDPGRRR ALRVQASLMT PVQPMLAEAC KSIEYAMKKC
510 520 530 540 550
PNGMFSEIKY DGERVQVHKK GDHFSYFSRS LKPVLPHKVA HFKDYIPKAF
560 570 580 590 600
PGGQSMILDS EVLLIDNNTG KPLPFGTLGV HKKAAFQDAN VCLFVFDCIY
610 620 630 640 650
FNDVSLMDRP LCERRKFLHD NMVEIRNRIM FSEMKQVTKA SDLADMINRV
660 670 680 690 700
IREGLEGLVL KDVKGTYEPG KRHWLKVKKD YLNEGAMADT ADLVVLGAFY
710 720 730 740 750
GQGSKGGMMS IFLMGCYDPD SQKWCTVTKC AGGHDDATLA RLQKELVMVK
760 770 780 790 800
ISKDPSKIPS WLKINKIYYP DFIVPDPKKA AVWEITGAEF SRSEAHTADG
810 820 830 840 850
ISIRFPRCTR IRDDKDWKSA TNLPQLKELY QLSKDKADFA VVAGDEASPT
860 870 880 890 900
TGGSSGENEG TAGSAGPCKG PPSKSSASAK TTEQKLNSPS SRGGIKPIPK
910 920 930 940 950
HSPMKPGEKL AVKSSPVKVG MKRKATDETP CLKKVLLDVF TGVRLYLPPS
960 970 980 990 1000
TPDFKRLKRY FVAFDGDLVQ EFDMGSATHV LGNREKNTDA QLVSSEWIWA
1010
CIRKRRLIAP C
Length:1,011
Mass (Da):112,710
Last modified:October 11, 2005 - v1
Checksum:iFDE0301DC5CCE099
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645594 Genomic DNA. No translation available.
AK150644 mRNA. Translation: BAE29732.1.
RefSeqiNP_001278175.1. NM_001291246.1.
NP_001278176.1. NM_001291247.1.
UniGeneiMm.277136.

Genome annotation databases

EnsembliENSMUST00000173347; ENSMUSP00000134300; ENSMUSG00000020697.
ENSMUST00000173727; ENSMUSP00000133849; ENSMUSG00000020697.
GeneIDi16882.
UCSCiuc007knb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645594 Genomic DNA. No translation available.
AK150644 mRNA. Translation: BAE29732.1.
RefSeqiNP_001278175.1. NM_001291246.1.
NP_001278176.1. NM_001291247.1.
UniGeneiMm.277136.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000090525.

Proteomic databases

PeptideAtlasiQ3UC82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000173347; ENSMUSP00000134300; ENSMUSG00000020697.
ENSMUST00000173727; ENSMUSP00000133849; ENSMUSG00000020697.
GeneIDi16882.
UCSCiuc007knb.2. mouse.

Organism-specific databases

CTDi3980.
MGIiMGI:109152. Lig3.

Phylogenomic databases

eggNOGiKOG4437. Eukaryota.
COG1793. LUCA.
GeneTreeiENSGT00840000129855.
HOVERGENiHBG005515.

Enzyme and pathway databases

ReactomeiR-MMU-110381. Resolution of AP sites via the single-nucleotide replacement pathway.
R-MMU-5649702. APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway.
R-MMU-5685939. HDR through MMEJ (alt-NHEJ).
R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Miscellaneous databases

ChiTaRSiLig3. mouse.
SOURCEiSearch...

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.30.1740.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR031916. LIG3_BRCT.
IPR012340. NA-bd_OB-fold.
IPR001510. Znf_PARP.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
PF16759. LIG3_BRCT. 1 hit.
PF00645. zf-PARP. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 1 hit.
SM01336. zf-PARP. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
PS00347. PARP_ZN_FINGER_1. 1 hit.
PS50064. PARP_ZN_FINGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "High-efficiency full-length cDNA cloning."
    Carninci P., Hayashizaki Y.
    Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Bone marrowImported.
  2. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
    Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
    Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Bone marrowImported.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Bone marrowImported.
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Bone marrowImported.
  5. "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
    The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
    Nature 420:563-573(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Bone marrowImported.
  6. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Bone marrowImported.
  7. "The Transcriptional Landscape of the Mammalian Genome."
    The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
    Science 309:1559-1563(2005)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Bone marrowImported.
  8. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Bone marrowImported.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6JImported.
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. Ensembl
    Submitted (FEB-2012) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: C57BL/6JImported.

Entry informationi

Entry nameiQ3UC82_MOUSE
AccessioniPrimary (citable) accession number: Q3UC82
Entry historyi
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.