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Protein

Transcription factor IIIB 50 kDa subunit

Gene

Brf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71ZincBy similarity
Metal bindingi10 – 101ZincBy similarity
Metal bindingi28 – 281ZincBy similarity
Metal bindingi31 – 311ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3634TFIIB-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 50 kDa subunit
Alternative name(s):
B-related factor 2
Short name:
BRF-2
Gene namesi
Name:Brf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913903. Brf2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Transcription factor IIIB 50 kDa subunitPRO_0000337189Add
BLAST

Proteomic databases

PaxDbiQ3UAW9.
PRIDEiQ3UAW9.

PTM databases

iPTMnetiQ3UAW9.
PhosphoSiteiQ3UAW9.

Expressioni

Gene expression databases

BgeeiQ3UAW9.

Interactioni

Subunit structurei

Component of TFIIIB complex. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. BRF2 recruitment to the TATA box containing small nuclear RNA (snRNA) gene templates is TBP-dependent. Interacts with TBP; this interaction with TBP mediates its TATA-box recruitment of these promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033877.

Structurei

3D structure databases

ProteinModelPortaliQ3UAW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati72 – 157861Add
BLAST
Repeati173 – 249772Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi322 – 33615Gln-richAdd
BLAST

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri3 – 3634TFIIB-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1598. Eukaryota.
COG1405. LUCA.
HOGENOMiHOG000095260.
HOVERGENiHBG107537.
InParanoidiQ3UAW9.
KOiK15197.
OrthoDBiEOG773XGF.
PhylomeDBiQ3UAW9.
TreeFamiTF331596.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 1 hit.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UAW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNGSRCPDC GSSELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNF
60 70 80 90 100
REVTYSRSTG ENEQVSRCQQ RDLRRVRDLC RILKLPLTFE DTAISYYQKA
110 120 130 140 150
YQLSGIRAAR LQKKEVLVGC CVLITCRQHN WPLTMGTICT LLYADLDLFS
160 170 180 190 200
GTYMQMVKLL GLDVPSLCLA DLVKSYCSSF KLFQASPSVP AKYVEDKDKM
210 220 230 240 250
LSRTLLLVEL ADETWLVTGR HPLPIITAAT FLAWQSLRPS DRLTCSLAQF
260 270 280 290 300
CKLANVDLPY PAASRLQELL AVLLQMAGQL AWLQVLKLNK RSVVKHIGDL
310 320 330 340 350
LQHRHMLVRT AFRDGTAEVE TQQQQQQQQG QGQGQQDEVG DGPFDLPKRK
360 370 380 390 400
RRASPTPLLP PCMLKPPKRT HTLPPESAVT GDEDISDSEI EQYLRTPQEV
410 420
RDFERAQAAS QAAMRVPNPP
Length:420
Mass (Da):47,050
Last modified:October 11, 2005 - v1
Checksum:i9B1D662EF90302F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti352 – 3521R → P in BAB28406 (PubMed:16141072).Curated
Sequence conflicti352 – 3521R → P in BAC25827 (PubMed:16141072).Curated
Sequence conflicti352 – 3521R → P in AAH98232 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012680 mRNA. Translation: BAB28406.1.
AK028228 mRNA. Translation: BAC25827.1.
AK151198 mRNA. Translation: BAE30195.1.
BC098232 mRNA. Translation: AAH98232.1.
CCDSiCCDS22211.1.
RefSeqiNP_079962.1. NM_025686.2.
UniGeneiMm.334467.

Genome annotation databases

GeneIDi66653.
KEGGimmu:66653.
UCSCiuc009lhy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012680 mRNA. Translation: BAB28406.1.
AK028228 mRNA. Translation: BAC25827.1.
AK151198 mRNA. Translation: BAE30195.1.
BC098232 mRNA. Translation: AAH98232.1.
CCDSiCCDS22211.1.
RefSeqiNP_079962.1. NM_025686.2.
UniGeneiMm.334467.

3D structure databases

ProteinModelPortaliQ3UAW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033877.

PTM databases

iPTMnetiQ3UAW9.
PhosphoSiteiQ3UAW9.

Proteomic databases

PaxDbiQ3UAW9.
PRIDEiQ3UAW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi66653.
KEGGimmu:66653.
UCSCiuc009lhy.1. mouse.

Organism-specific databases

CTDi55290.
MGIiMGI:1913903. Brf2.

Phylogenomic databases

eggNOGiKOG1598. Eukaryota.
COG1405. LUCA.
HOGENOMiHOG000095260.
HOVERGENiHBG107537.
InParanoidiQ3UAW9.
KOiK15197.
OrthoDBiEOG773XGF.
PhylomeDBiQ3UAW9.
TreeFamiTF331596.

Miscellaneous databases

PROiQ3UAW9.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UAW9.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 1 hit.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Lung.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Spleen.

Entry informationi

Entry nameiBRF2_MOUSE
AccessioniPrimary (citable) accession number: Q3UAW9
Secondary accession number(s): Q9CZF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 11, 2005
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.