UniProtKB - Q3UAW9 (BRF2_MOUSE)
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Protein
Transcription factor IIIB 50 kDa subunit
Gene
Brf2
Organism
Mus musculus (Mouse)
Status
Functioni
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 7 | ZincBy similarity | 1 | |
Metal bindingi | 10 | ZincBy similarity | 1 | |
Metal bindingi | 28 | ZincBy similarity | 1 | |
Metal bindingi | 31 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 3 – 36 | TFIIB-typePROSITE-ProRule annotationAdd BLAST | 34 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- RNA polymerase III type 3 promoter DNA binding Source: UniProtKB
- transcription factor activity, RNA polymerase III transcription factor binding Source: UniProtKB
GO - Biological processi
- cellular response to oxidative stress Source: UniProtKB
- DNA-templated transcriptional preinitiation complex assembly Source: InterPro
- regulation of transcription by RNA polymerase III Source: UniProtKB
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription factor IIIB 50 kDa subunitAlternative name(s): B-related factor 2 Short name: BRF-2 |
Gene namesi | Name:Brf2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913903. Brf2. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000337189 | 1 – 420 | Transcription factor IIIB 50 kDa subunitAdd BLAST | 420 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 354 | PhosphoserineBy similarity | 1 | |
Modified residuei | 362 | Cysteine sulfenic acid (-SOH)By similarity | 1 |
Post-translational modificationi
In response to oxidative stress, Cys-362 is reversibly oxidized to cysteine sulfenic acid. Oxidation of Cys-362 impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress.By similarity
Keywords - PTMi
Oxidation, PhosphoproteinProteomic databases
PaxDbi | Q3UAW9. |
PRIDEi | Q3UAW9. |
PTM databases
iPTMneti | Q3UAW9. |
PhosphoSitePlusi | Q3UAW9. |
Interactioni
Subunit structurei
Component of TFIIIB complexes. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. Interacts with TBP; this interaction promotes recruitment of BRF2 to TATA box-containing promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.By similarity
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000033877. |
Structurei
3D structure databases
ProteinModelPortali | Q3UAW9. |
SMRi | Q3UAW9. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 72 – 157 | 1Add BLAST | 86 | |
Repeati | 173 – 249 | 2Add BLAST | 77 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 108 – 114 | Interaction with target DNABy similarity | 7 | |
Regioni | 358 – 364 | Required for the formation of a ternary complex with DNA and TBP; not required for interaction with TBP in the absence of DNABy similarity | 7 | |
Regioni | 366 – 420 | Required for interaction with TBP and formation of a ternary complex with DNA and TBPBy similarityAdd BLAST | 55 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 322 – 336 | Gln-richAdd BLAST | 15 |
Sequence similaritiesi
Belongs to the TFIIB family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 3 – 36 | TFIIB-typePROSITE-ProRule annotationAdd BLAST | 34 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1598. Eukaryota. COG1405. LUCA. |
HOGENOMi | HOG000095260. |
HOVERGENi | HBG107537. |
InParanoidi | Q3UAW9. |
KOi | K15197. |
PhylomeDBi | Q3UAW9. |
TreeFami | TF331596. |
Family and domain databases
InterProi | View protein in InterPro IPR036915. Cyclin-like_sf. IPR000812. TFIIB. IPR013137. Znf_TFIIB. |
PANTHERi | PTHR11618. PTHR11618. 1 hit. |
Pfami | View protein in Pfam PF08271. TF_Zn_Ribbon. 1 hit. |
SUPFAMi | SSF47954. SSF47954. 1 hit. |
PROSITEi | View protein in PROSITE PS51134. ZF_TFIIB. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q3UAW9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPNGSRCPDC GSSELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNF
60 70 80 90 100
REVTYSRSTG ENEQVSRCQQ RDLRRVRDLC RILKLPLTFE DTAISYYQKA
110 120 130 140 150
YQLSGIRAAR LQKKEVLVGC CVLITCRQHN WPLTMGTICT LLYADLDLFS
160 170 180 190 200
GTYMQMVKLL GLDVPSLCLA DLVKSYCSSF KLFQASPSVP AKYVEDKDKM
210 220 230 240 250
LSRTLLLVEL ADETWLVTGR HPLPIITAAT FLAWQSLRPS DRLTCSLAQF
260 270 280 290 300
CKLANVDLPY PAASRLQELL AVLLQMAGQL AWLQVLKLNK RSVVKHIGDL
310 320 330 340 350
LQHRHMLVRT AFRDGTAEVE TQQQQQQQQG QGQGQQDEVG DGPFDLPKRK
360 370 380 390 400
RRASPTPLLP PCMLKPPKRT HTLPPESAVT GDEDISDSEI EQYLRTPQEV
410 420
RDFERAQAAS QAAMRVPNPP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 352 | R → P in BAB28406 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 352 | R → P in BAC25827 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 352 | R → P in AAH98232 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK012680 mRNA. Translation: BAB28406.1. AK028228 mRNA. Translation: BAC25827.1. AK151198 mRNA. Translation: BAE30195.1. BC098232 mRNA. Translation: AAH98232.1. |
CCDSi | CCDS22211.1. |
RefSeqi | NP_079962.1. NM_025686.2. |
UniGenei | Mm.334467. |
Genome annotation databases
GeneIDi | 66653. |
KEGGi | mmu:66653. |
UCSCi | uc009lhy.1. mouse. |
Similar proteinsi
Entry informationi
Entry namei | BRF2_MOUSE | |
Accessioni | Q3UAW9Primary (citable) accession number: Q3UAW9 Secondary accession number(s): Q9CZF0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 20, 2008 |
Last sequence update: | October 11, 2005 | |
Last modified: | March 28, 2018 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |