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Protein

Transcription factor IIIB 50 kDa subunit

Gene

Brf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7ZincBy similarity1
Metal bindingi10ZincBy similarity1
Metal bindingi28ZincBy similarity1
Metal bindingi31ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 36TFIIB-typePROSITE-ProRule annotationAdd BLAST34

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIB 50 kDa subunit
Alternative name(s):
B-related factor 2
Short name:
BRF-2
Gene namesi
Name:Brf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913903. Brf2.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003371891 – 420Transcription factor IIIB 50 kDa subunitAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphoserineBy similarity1
Modified residuei362Cysteine sulfenic acid (-SOH)By similarity1

Post-translational modificationi

In response to oxidative stress, Cys-362 is reversibly oxidized to cysteine sulfenic acid. Oxidation of Cys-362 impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress.By similarity

Keywords - PTMi

Oxidation, Phosphoprotein

Proteomic databases

PaxDbiQ3UAW9.
PRIDEiQ3UAW9.

PTM databases

iPTMnetiQ3UAW9.
PhosphoSitePlusiQ3UAW9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031487.

Interactioni

Subunit structurei

Component of TFIIIB complexes. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. Interacts with TBP; this interaction promotes recruitment of BRF2 to TATA box-containing promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033877.

Structurei

3D structure databases

ProteinModelPortaliQ3UAW9.
SMRiQ3UAW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 1571Add BLAST86
Repeati173 – 2492Add BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 114Interaction with target DNABy similarity7
Regioni358 – 364Required for the formation of a ternary complex with DNA and TBP; not required for interaction with TBP in the absence of DNABy similarity7
Regioni366 – 420Required for interaction with TBP and formation of a ternary complex with DNA and TBPBy similarityAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi322 – 336Gln-richAdd BLAST15

Sequence similaritiesi

Belongs to the TFIIB family.Curated
Contains 1 TFIIB-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri3 – 36TFIIB-typePROSITE-ProRule annotationAdd BLAST34

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1598. Eukaryota.
COG1405. LUCA.
HOGENOMiHOG000095260.
HOVERGENiHBG107537.
InParanoidiQ3UAW9.
KOiK15197.
PhylomeDBiQ3UAW9.
TreeFamiTF331596.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 1 hit.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UAW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNGSRCPDC GSSELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNF
60 70 80 90 100
REVTYSRSTG ENEQVSRCQQ RDLRRVRDLC RILKLPLTFE DTAISYYQKA
110 120 130 140 150
YQLSGIRAAR LQKKEVLVGC CVLITCRQHN WPLTMGTICT LLYADLDLFS
160 170 180 190 200
GTYMQMVKLL GLDVPSLCLA DLVKSYCSSF KLFQASPSVP AKYVEDKDKM
210 220 230 240 250
LSRTLLLVEL ADETWLVTGR HPLPIITAAT FLAWQSLRPS DRLTCSLAQF
260 270 280 290 300
CKLANVDLPY PAASRLQELL AVLLQMAGQL AWLQVLKLNK RSVVKHIGDL
310 320 330 340 350
LQHRHMLVRT AFRDGTAEVE TQQQQQQQQG QGQGQQDEVG DGPFDLPKRK
360 370 380 390 400
RRASPTPLLP PCMLKPPKRT HTLPPESAVT GDEDISDSEI EQYLRTPQEV
410 420
RDFERAQAAS QAAMRVPNPP
Length:420
Mass (Da):47,050
Last modified:October 11, 2005 - v1
Checksum:i9B1D662EF90302F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti352R → P in BAB28406 (PubMed:16141072).Curated1
Sequence conflicti352R → P in BAC25827 (PubMed:16141072).Curated1
Sequence conflicti352R → P in AAH98232 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012680 mRNA. Translation: BAB28406.1.
AK028228 mRNA. Translation: BAC25827.1.
AK151198 mRNA. Translation: BAE30195.1.
BC098232 mRNA. Translation: AAH98232.1.
CCDSiCCDS22211.1.
RefSeqiNP_079962.1. NM_025686.2.
UniGeneiMm.334467.

Genome annotation databases

GeneIDi66653.
KEGGimmu:66653.
UCSCiuc009lhy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012680 mRNA. Translation: BAB28406.1.
AK028228 mRNA. Translation: BAC25827.1.
AK151198 mRNA. Translation: BAE30195.1.
BC098232 mRNA. Translation: AAH98232.1.
CCDSiCCDS22211.1.
RefSeqiNP_079962.1. NM_025686.2.
UniGeneiMm.334467.

3D structure databases

ProteinModelPortaliQ3UAW9.
SMRiQ3UAW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033877.

PTM databases

iPTMnetiQ3UAW9.
PhosphoSitePlusiQ3UAW9.

Proteomic databases

PaxDbiQ3UAW9.
PRIDEiQ3UAW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi66653.
KEGGimmu:66653.
UCSCiuc009lhy.1. mouse.

Organism-specific databases

CTDi55290.
MGIiMGI:1913903. Brf2.

Phylogenomic databases

eggNOGiKOG1598. Eukaryota.
COG1405. LUCA.
HOGENOMiHOG000095260.
HOVERGENiHBG107537.
InParanoidiQ3UAW9.
KOiK15197.
PhylomeDBiQ3UAW9.
TreeFamiTF331596.

Miscellaneous databases

PROiQ3UAW9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031487.

Family and domain databases

Gene3Di1.10.472.10. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 1 hit.
PROSITEiPS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBRF2_MOUSE
AccessioniPrimary (citable) accession number: Q3UAW9
Secondary accession number(s): Q9CZF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.