Brf2

Functionⁱ

General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	7	ZincBy similarity	1
Metal bindingⁱ	10	ZincBy similarity	1
Metal bindingⁱ	28	ZincBy similarity	1
Metal bindingⁱ	31	ZincBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	3 – 36	TFIIB-typePROSITE-ProRule annotationAdd BLAST		34

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
RNA polymerase III type 3 promoter DNA binding Source: UniProtKB
transcription factor activity, RNA polymerase III transcription factor binding Source: UniProtKB

Complete GO annotation...

GO - Biological processⁱ

cellular response to oxidative stress Source: UniProtKB
DNA-templated transcriptional preinitiation complex assembly Source: InterPro
regulation of transcription from RNA polymerase III promoter Source: UniProtKB

Complete GO annotation...

Keywordsⁱ

Molecular function	Activator
Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription factor IIIB 50 kDa subunit Alternative name(s): B-related factor 2 Short name: BRF-2
Gene namesⁱ	Name:Brf2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Unplaced

Organism-specific databases

MGIⁱ	MGI:1913903. Brf2.

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000337189	1 – 420	Transcription factor IIIB 50 kDa subunitAdd BLAST		420

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	354	PhosphoserineBy similarity		1
Modified residueⁱ	362	Cysteine sulfenic acid (-SOH)By similarity		1

Post-translational modificationⁱ

In response to oxidative stress, Cys-362 is reversibly oxidized to cysteine sulfenic acid. Oxidation of Cys-362 impairs formation of a ternary complex with TBP and DNA and down-regulates expression of target genes in response to oxidative stress.By similarity

Keywords - PTMⁱ

Oxidation, Phosphoprotein

Proteomic databases

PaxDbⁱ	Q3UAW9.
PRIDEⁱ	Q3UAW9.

PTM databases

iPTMnetⁱ	Q3UAW9.
PhosphoSitePlusⁱ	Q3UAW9.

Interactionⁱ

Subunit structureⁱ

Component of TFIIIB complexes. The TFIIIB complex has two activities, alpha and beta. The TFIIIB-alpha activity complex is composed of TBP, BDP1, and a complex containing both BRF2 and at least four stably associated proteins; this complex inhibits the transcription by pol III via its phosphorylation by CK2; YY1 facilitates the TFIIIB-alpha complex formation. Interacts with TBP; this interaction promotes recruitment of BRF2 to TATA box-containing promoters. Interacts with TBP and the BURE sequence (GC-rich sequence downstream from the TATA box) to form a strong ternary complex which is joined by BDP1; this ternary complex stimulates pol III transcription. Forms a trimeric complex composed of TBP, BRF2 and mini-SNAPc complex (SNAP43, SNAP50, and the N-terminal third of SNAP190) on the promoter. Assembly of the TBP-BRF2 complex is stimulated by SNAP190. Interacts with MAF1 and SNAPC4.By similarity

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000033877.

STRINGⁱ

10090.ENSMUSP00000033877.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q3UAW9.
SMRⁱ	Q3UAW9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Repeatⁱ	72 – 157	1Add BLAST		86
Repeatⁱ	173 – 249	2Add BLAST		77

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	108 – 114	Interaction with target DNABy similarity	7
Regionⁱ	358 – 364	Required for the formation of a ternary complex with DNA and TBP; not required for interaction with TBP in the absence of DNABy similarity	7
Regionⁱ	366 – 420	Required for interaction with TBP and formation of a ternary complex with DNA and TBPBy similarityAdd BLAST	55

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	322 – 336	Gln-richAdd BLAST		15

Sequence similaritiesⁱ

Belongs to the TFIIB family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	3 – 36	TFIIB-typePROSITE-ProRule annotationAdd BLAST		34

Keywords - Domainⁱ

Repeat, Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1598. Eukaryota. COG1405. LUCA.
HOGENOMⁱ	HOG000095260.
HOVERGENⁱ	HBG107537.
InParanoidⁱ	Q3UAW9.
KEGG Orthology (KO) More...KOⁱ	K15197.
PhylomeDBⁱ	Q3UAW9.
TreeFamⁱ	TF331596.

Family and domain databases

Gene3Dⁱ	1.10.472.10. 2 hits.
InterProⁱ	View protein in InterPro IPR013763. Cyclin-like. IPR000812. TFIIB. IPR013137. Znf_TFIIB.
PANTHERⁱ	PTHR11618. PTHR11618. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08271. TF_Zn_Ribbon. 1 hit.
SUPFAMⁱ	SSF47954. SSF47954. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51134. ZF_TFIIB. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q3UAW9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPNGSRCPDC GSSELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNF 
        60         70         80         90        100
REVTYSRSTG ENEQVSRCQQ RDLRRVRDLC RILKLPLTFE DTAISYYQKA 
       110        120        130        140        150
YQLSGIRAAR LQKKEVLVGC CVLITCRQHN WPLTMGTICT LLYADLDLFS 
       160        170        180        190        200
GTYMQMVKLL GLDVPSLCLA DLVKSYCSSF KLFQASPSVP AKYVEDKDKM 
       210        220        230        240        250
LSRTLLLVEL ADETWLVTGR HPLPIITAAT FLAWQSLRPS DRLTCSLAQF 
       260        270        280        290        300
CKLANVDLPY PAASRLQELL AVLLQMAGQL AWLQVLKLNK RSVVKHIGDL 
       310        320        330        340        350
LQHRHMLVRT AFRDGTAEVE TQQQQQQQQG QGQGQQDEVG DGPFDLPKRK 
       360        370        380        390        400
RRASPTPLLP PCMLKPPKRT HTLPPESAVT GDEDISDSEI EQYLRTPQEV 
       410        420
RDFERAQAAS QAAMRVPNPP

Length:420

Mass (Da):47,050

Last modified:October 11, 2005 - v1

Checksum:ⁱ9B1D662EF90302F8

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	352	R → P in BAB28406 (PubMed:16141072).Curated	1
Sequence conflictⁱ	352	R → P in BAC25827 (PubMed:16141072).Curated	1
Sequence conflictⁱ	352	R → P in AAH98232 (PubMed:15489334).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK012680 mRNA. Translation: BAB28406.1. AK028228 mRNA. Translation: BAC25827.1. AK151198 mRNA. Translation: BAE30195.1. BC098232 mRNA. Translation: AAH98232.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS22211.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_079962.1. NM_025686.2.
UniGeneⁱ	Mm.334467.

Genome annotation databases

GeneIDⁱ	66653.
KEGGⁱ	mmu:66653.
UCSC genome browser More...UCSCⁱ	uc009lhy.1. mouse.

Similar proteinsⁱ

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q3UAW9	A0A0R4J0C6 UPI0003D6F504 UPI0003D76F4D Q4V8D6 UPI000A31A022 UPI000A31156C	Mus musculus (Mouse) Rattus norvegicus (Rat) Mus caroli (Ryukyu mouse) (Ricefield mouse) Mus pahari (Gairdner's shrew-mouse) (Coelomys pahari)	420	UniRef90_Q3UAW9	Cluster: Transcription factor IIIB 50 kDa subunit	7

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q3UAW9	Q9HAW0 H2QW11 UPI00057BBFAD UPI0003C89D75 H9ZAV1 UPI0005F40D22 UPI00027FAC96 A0A0D9RSN2 F7EEK0 G7PD66 +147	Homo sapiens (Human) Pan troglodytes (Chimpanzee) Camelus dromedarius (Dromedary) (Arabian camel) Camelus bactrianus (Bactrian camel) Macaca mulatta (Rhesus macaque) Colobus angolensis palliatus (Peters' Angolan colobus) Saimiri boliviensis boliviensis (Bolivian squirrel monkey) Chlorocebus sabaeus (Green monkey) (Cercopithecus sabaeus) Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Gorilla gorilla gorilla (Western lowland gorilla) And more	419	UniRef50_Q9HAW0	Cluster: Transcription factor IIIB 50 kDa subunit	158

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK012680 mRNA. Translation: BAB28406.1. AK028228 mRNA. Translation: BAC25827.1. AK151198 mRNA. Translation: BAE30195.1. BC098232 mRNA. Translation: AAH98232.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS22211.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_079962.1. NM_025686.2.
UniGeneⁱ	Mm.334467.

3D structure databases

ProteinModelPortalⁱ	Q3UAW9.
SMRⁱ	Q3UAW9.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000033877.

STRINGⁱ

10090.ENSMUSP00000033877.

PTM databases

iPTMnetⁱ	Q3UAW9.
PhosphoSitePlusⁱ	Q3UAW9.

Proteomic databases

PaxDbⁱ	Q3UAW9.
PRIDEⁱ	Q3UAW9.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	66653.
KEGGⁱ	mmu:66653.
UCSC genome browser More...UCSCⁱ	uc009lhy.1. mouse.

Organism-specific databases

CTDⁱ	55290.
MGIⁱ	MGI:1913903. Brf2.

Phylogenomic databases

eggNOGⁱ	KOG1598. Eukaryota. COG1405. LUCA.
HOGENOMⁱ	HOG000095260.
HOVERGENⁱ	HBG107537.
InParanoidⁱ	Q3UAW9.
KEGG Orthology (KO) More...KOⁱ	K15197.
PhylomeDBⁱ	Q3UAW9.
TreeFamⁱ	TF331596.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q3UAW9.
SOURCEⁱ	Search...

Family and domain databases

Gene3Dⁱ	1.10.472.10. 2 hits.
InterProⁱ	View protein in InterPro IPR013763. Cyclin-like. IPR000812. TFIIB. IPR013137. Znf_TFIIB.
PANTHERⁱ	PTHR11618. PTHR11618. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08271. TF_Zn_Ribbon. 1 hit.
SUPFAMⁱ	SSF47954. SSF47954. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51134. ZF_TFIIB. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	BRF2_MOUSE
Accessionⁱ	Q3UAW9Primary (citable) accession number: Q3UAW9 Secondary accession number(s): Q9CZF0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
	Last sequence update:	October 11, 2005
	Last modified:	August 30, 2017
	This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q3UAW9 (BRF2_MOUSE)

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Transcription factor IIIB 50 kDa subunit

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ