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Q3U9N9 (MOT10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monocarboxylate transporter 10

Short name=MCT 10
Alternative name(s):
Aromatic amino acid transporter 1
Solute carrier family 16 member 10
T-type amino acid transporter 1
Gene names
Name:Slc16a10
Synonyms:Mct10, Tat1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-independent transporter that mediates the update of aromatic acid. Can function as a net efflux pathway for aromatic amino acids in the basosolateral epithelial cells. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein. Basolateral cell membrane. Note: Detected in basolateral membrane of kidney proximal tubule starting at he beginning of the proximal tubule epithelium within the glomerular capsule and extending to the S1 and S2 segments. Localizes also to the basolateral membrane of small intestine enterocytes and to sinusoidal side of perivenous hepatocytes. Ref.2

Tissue specificity

Highly expressed in small intestine, particularly in jejunum and ileum, scarcely in colon and substantially in kidney, liver and skeletal muscle. Ref.2

Post-translational modification

Not N-glycosylated. Ref.2

Sequence similarities

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]

Sequence caution

The sequence BAE38097.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtransmembrane transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbasolateral plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3U9N9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3U9N9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     312-320: MNHVKERFQ → VSMHLWLCL
     321-512: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Monocarboxylate transporter 10
PRO_0000314254

Regions

Topological domain1 – 6363Extracellular Potential
Transmembrane64 – 8421Helical; Potential
Topological domain85 – 11127Cytoplasmic Potential
Transmembrane112 – 13221Helical; Potential
Topological domain133 – 1419Extracellular Potential
Transmembrane142 – 16221Helical; Potential
Topological domain163 – 1686Cytoplasmic Potential
Transmembrane169 – 18921Helical; Potential
Topological domain190 – 20112Extracellular Potential
Transmembrane202 – 22221Helical; Potential
Topological domain223 – 23210Cytoplasmic Potential
Transmembrane233 – 25321Helical; Potential
Topological domain254 – 29138Extracellular Potential
Transmembrane292 – 31221Helical; Potential
Topological domain313 – 32614Cytoplasmic Potential
Transmembrane327 – 34721Helical; Potential
Topological domain348 – 36215Extracellular Potential
Transmembrane363 – 38321Helical; Potential
Topological domain3841Cytoplasmic Potential
Transmembrane385 – 40521Helical; Potential
Topological domain406 – 41611Extracellular Potential
Transmembrane417 – 43721Helical; Potential
Topological domain438 – 44811Cytoplasmic Potential
Transmembrane449 – 46921Helical; Potential
Topological domain470 – 51243Extracellular Potential

Amino acid modifications

Modified residue4951Phosphoserine Ref.4
Modified residue4981Phosphoserine Ref.4
Modified residue5011Phosphoserine Ref.4

Natural variations

Alternative sequence312 – 3209MNHVKERFQ → VSMHLWLCL in isoform 2.
VSP_030252
Alternative sequence321 – 512192Missing in isoform 2.
VSP_030253

Experimental info

Sequence conflict2111T → I in BAE30275. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 4EF7A1400C8E0B04

FASTA51255,320
        10         20         30         40         50         60 
MVPSQEEPAA ERETNEAQPP GPAPSDDAPL PGPGPSDVSD VAAEKVEVEL TRSAGSEPPV 

        70         80         90        100        110        120 
PPEGGWGWLV MLAAMWCNGS VFGIQNAYGV LFVSMLDTFK AKDDDNMAFK TAWVGSLSMG 

       130        140        150        160        170        180 
MIFFCCPIVS VFTDMFGCRR TAVVGAAVGF IGLMSSSFVS SIEPLYLTYG IIFACGCSFA 

       190        200        210        220        230        240 
YQPSLVILGH YFKKRLGLVN GIVTAGSSVF TILLPLLLGN LISSVKLFNT LRILCIFMFV 

       250        260        270        280        290        300 
LFLAGFTYRP LVPSTKEKES GGSRSSFFSR RKLSPPKKVF NFALFKETTY AVWAAGIPLA 

       310        320        330        340        350        360 
LFGYFVPYVH LMNHVKERFQ DVNNKEVLFM CIGITSGVGR LLFGRIADYL PGVKKVYLQV 

       370        380        390        400        410        420 
LSFFFIGLMS MMIPLCSAFG ALIAVCLAMG LFDGCFISIM APIAFELVGP QDASQAIGFL 

       430        440        450        460        470        480 
LGFMSIPMTV GPPIAGLLHD KLGTYDVAFY LAGIPPFVGG VVLCLIPWIH SKKQRKISKN 

       490        500        510 
AGGEKMEKML ENQSSLLSGS SGIFKKDSAS II 

« Hide

Isoform 2 [UniParc].

Checksum: A60696B0242BDF35
Show »

FASTA32034,718

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Bone marrow and Spleen.
[2]"Basolateral aromatic amino acid transporter TAT1 (Slc16a10) functions as an efflux pathway."
Ramadan T., Camargo S.M., Summa V., Hunziker P., Chesnov S., Pos K.M., Verrey F.
J. Cell. Physiol. 206:771-779(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION, LACK OF GLYCOSYLATION.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495; SER-498 AND SER-501, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK050229 mRNA. Translation: BAC34135.1.
AK151290 mRNA. Translation: BAE30275.1.
AK151704 mRNA. Translation: BAE30627.1.
AK157355 mRNA. Translation: BAE34061.1.
AK165240 mRNA. Translation: BAE38097.1. Different initiation.
AK165579 mRNA. Translation: BAE38270.1.
CCDSCCDS48543.1. [Q3U9N9-1]
RefSeqNP_001107804.1. NM_001114332.1. [Q3U9N9-1]
NP_082523.1. NM_028247.4. [Q3U9N9-2]
UniGeneMm.186778.

3D structure databases

ProteinModelPortalQ3U9N9.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

MaxQBQ3U9N9.
PaxDbQ3U9N9.
PRIDEQ3U9N9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000092566; ENSMUSP00000090227; ENSMUSG00000019838. [Q3U9N9-1]
GeneID72472.
KEGGmmu:72472.
UCSCuc007ewm.2. mouse. [Q3U9N9-1]
uc007ewn.2. mouse. [Q3U9N9-2]

Organism-specific databases

CTD117247.
MGIMGI:1919722. Slc16a10.

Phylogenomic databases

eggNOGNOG321880.
GeneTreeENSGT00740000115479.
HOGENOMHOG000046644.
HOVERGENHBG006387.
InParanoidQ3U9N9.
KOK08187.
OMAHLMKHVN.
OrthoDBEOG7KWSH8.
PhylomeDBQ3U9N9.
TreeFamTF313792.

Gene expression databases

BgeeQ3U9N9.
GenevestigatorQ3U9N9.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio336298.
PROQ3U9N9.
SOURCESearch...

Entry information

Entry nameMOT10_MOUSE
AccessionPrimary (citable) accession number: Q3U9N9
Secondary accession number(s): Q3TNJ1, Q3UAN9, Q8BWR7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot