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Protein

Histone-lysine N-methyltransferase KMT5B

Gene

Kmt5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Plays a role in myogenesis by regulating the expression of target genes, such as EID3.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].PROSITE-ProRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Myogenesis, Transcription, Transcription regulation

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-3214841. PKMTs methylate histone lysines.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase KMT5BCurated (EC:2.1.1.43)
Alternative name(s):
Lysine-specific methyltransferase 5BBy similarity
Suppressor of variegation 4-20 homolog 1
Short name:
Su(var)4-20 homolog 1
Short name:
Suv4-20h1
Gene namesi
Name:Kmt5bBy similarity
Synonyms:Suv420h1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2444557. Suv420h1.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome, centromeric region Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Partial muscle-specific knockout mice display several signs of muscular dystrophy including necrosis and an increased number of centrally nucleated myofibers. RNAi-mediated knockdown in C2C12 muscle cells causes reduced myogenic differentation of the cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002817881 – 883Histone-lysine N-methyltransferase KMT5BAdd BLAST883

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki556Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ3U8K7.
PaxDbiQ3U8K7.
PeptideAtlasiQ3U8K7.
PRIDEiQ3U8K7.

PTM databases

iPTMnetiQ3U8K7.
PhosphoSitePlusiQ3U8K7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000045098.
ExpressionAtlasiQ3U8K7. baseline and differential.
GenevisibleiQ3U8K7. MM.

Interactioni

Subunit structurei

Interacts with HP1 proteins CBX1, CBX3 and CBX5. Interacts with RB1 family proteins RB1, RBL1 and RBL2. Interacts (via C-terminus) with FRG1.4 Publications

Protein-protein interaction databases

BioGridi230441. 3 interactors.
STRINGi10090.ENSMUSP00000109605.

Structurei

Secondary structure

1883
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi75 – 89Combined sources15
Helixi91 – 94Combined sources4
Helixi138 – 148Combined sources11
Helixi151 – 158Combined sources8
Helixi162 – 167Combined sources6
Helixi173 – 187Combined sources15
Helixi188 – 190Combined sources3
Beta strandi196 – 201Combined sources6
Beta strandi208 – 217Combined sources10
Beta strandi224 – 235Combined sources12
Helixi237 – 243Combined sources7
Turni246 – 248Combined sources3
Beta strandi253 – 256Combined sources4
Turni257 – 260Combined sources4
Beta strandi261 – 267Combined sources7
Helixi268 – 271Combined sources4
Beta strandi279 – 296Combined sources18
Turni310 – 313Combined sources4
Helixi315 – 317Combined sources3
Helixi323 – 328Combined sources6
Helixi331 – 333Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BUPX-ray2.17A/B70-336[»]
ProteinModelPortaliQ3U8K7.
SMRiQ3U8K7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini194 – 309SETPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2589. Eukaryota.
ENOG410XPH8. LUCA.
GeneTreeiENSGT00520000055640.
HOVERGENiHBG105761.
InParanoidiQ3U8K7.
KOiK11429.
OMAiSGCLTRH.
OrthoDBiEOG091G0IUY.
PhylomeDBiQ3U8K7.
TreeFamiTF106433.

Family and domain databases

InterProiIPR025790. Hist-Lys_N-MTase_Suvar4-20.
IPR001214. SET_dom.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
[Graphical view]
PROSITEiPS51570. SAM_MT43_SUVAR420_2. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3U8K7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKWLGDSKNM VVNGRRNGGK LSNDHQQNQS KLQQHSGKDT LKTGRNAVER
60 70 80 90 100
RSSRCHGNSG FEGQSRYVPS SGMSAKELCE NDDLATSLVL DPYLGFQTHK
110 120 130 140 150
MNTSAFPSRS SRHISKADSF SHNNPVRFRP IKGRQEELKE VIERFKKDEH
160 170 180 190 200
LEKAFKCLTS GEWARHYFLN KNKMQEKLFK EHVFIYLRMF ATDSGFEILP
210 220 230 240 250
CNRYSSEQNG AKIVATKEWK RNDKIELLVG CIAELSEIEE NMLLRHGEND
260 270 280 290 300
FSVMYSTRKN CAQLWLGPAA FINHDCRPNC KFVSTGRDTA CVKALRDIEP
310 320 330 340 350
GEEISCYYGD GFFGENNEFC ECYTCERRGT GAFKSRVGLP APAPVINSKY
360 370 380 390 400
GLRETDKRLN RLKKLGDSSK NSDSQSVSSN TDADTTQEKD NATSNRKSSV
410 420 430 440 450
GVKKSSKSRA LTRPSMPRVP AASNSTSPKL VHTNNPRVPK KLRKPAKPLL
460 470 480 490 500
SKIRLRNHCK RLDQKSASRK LEMGSLVLKE PKVVLYKNLP IKKEREPEGP
510 520 530 540 550
AHAAVGSGCL TRHAAREHRQ NHGRGAHSQG DSLPCTYTTR RSLRTRTGLK
560 570 580 590 600
ETTDIKLEPS PLDGYKNGIL EPCPDSGQQP TPEVLEELAP ETAHREEASQ
610 620 630 640 650
ECPKNDSCLS RKKFRQVKPV KHLAKTEDCS PEHSFPGKDG LPDLPGSHPD
660 670 680 690 700
QGEPSGTVRV PVSHTDSAPS PVGCSVVAPD SFTKDSFRTA QSKKKRRVTR
710 720 730 740 750
YDAQLILENS SGIPKLTLRR RHDSSSKTND HESDGVNSSK ISIKLSKDHD
760 770 780 790 800
SDSNLYVAKL SNGVSAGPGS SSTKLKIQLK RDEESRGPCA EGLHENGVCC
810 820 830 840 850
SDPLSLLESQ MEVDDYSQYE EDSTDESSSS EGEEEEEDCE DDFDDDFIPL
860 870 880
PPAKRLRLIV GKDSIDIDIS SRRREDQSLR LNA
Length:883
Mass (Da):98,580
Last modified:April 3, 2007 - v2
Checksum:i2B24461F582CC5F1
GO
Isoform 2 (identifier: Q3U8K7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     393-394: TS → SK
     395-883: Missing.

Show »
Length:394
Mass (Da):44,723
Checksum:i1425ECDBE1F767D7
GO
Isoform 3 (identifier: Q3U8K7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     104-126: Missing.

Show »
Length:860
Mass (Da):96,070
Checksum:i2EEE457CEE91A890
GO
Isoform 4 (identifier: Q3U8K7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     328-883: Missing.

Show »
Length:327
Mass (Da):37,546
Checksum:i6400D6AAEEA26B99
GO
Isoform 5 (identifier: Q3U8K7-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     104-126: Missing.
     831-883: EGEEEEEDCEDDFDDDFIPLPPAKRLRLIVGKDSIDIDISSRRREDQSLRLNA → SGKAAEANCW

Note: No experimental confirmation available.
Show »
Length:817
Mass (Da):90,944
Checksum:i478C772F2EAFDEC4
GO

Sequence cautioni

The sequence AAH11214 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAT00539 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC39834 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE23934 differs from that shown. Reason: Erroneous termination at position 3. Translated as Trp.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53S → N in AAH75709 (PubMed:15489334).Curated1
Sequence conflicti153K → E in AAH11214 (PubMed:16141072).Curated1
Sequence conflicti292V → L in BAC39834 (PubMed:15145825).Curated1
Sequence conflicti328R → Q in BAE31010 (PubMed:16141072).Curated1
Sequence conflicti428P → A in AAH75709 (PubMed:15489334).Curated1
Sequence conflicti472E → G in BAC38817 (PubMed:15145825).Curated1
Sequence conflicti521N → S in AAH75709 (PubMed:15489334).Curated1
Sequence conflicti590P → H in BAC38817 (PubMed:15145825).Curated1
Sequence conflicti595R → G in BAE31010 (PubMed:16141072).Curated1
Sequence conflicti664H → Y in AAH75709 (PubMed:15489334).Curated1
Sequence conflicti678A → T in AAH75709 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_024053104 – 126Missing in isoform 3 and isoform 5. 2 PublicationsAdd BLAST23
Alternative sequenceiVSP_024054328 – 883Missing in isoform 4. 2 PublicationsAdd BLAST556
Alternative sequenceiVSP_024055393 – 394TS → SK in isoform 2. 1 Publication2
Alternative sequenceiVSP_024056395 – 883Missing in isoform 2. 1 PublicationAdd BLAST489
Alternative sequenceiVSP_024057831 – 883EGEEE…LRLNA → SGKAAEANCW in isoform 5. 1 PublicationAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY555192 mRNA. Translation: AAT00539.1. Different initiation.
AK036880 mRNA. Translation: BAC29617.1.
AK054093 mRNA. Translation: BAC35652.1.
AK082660 mRNA. Translation: BAC38565.1.
AK083227 mRNA. Translation: BAC38817.1.
AK087267 mRNA. Translation: BAC39834.1. Different initiation.
AK139255 mRNA. Translation: BAE23934.1. Sequence problems.
AK152179 mRNA. Translation: BAE31010.1.
BC011214 mRNA. Translation: AAH11214.1. Different initiation.
BC075709 mRNA. Translation: AAH75709.1.
CCDSiCCDS29399.1. [Q3U8K7-3]
CCDS50343.1. [Q3U8K7-1]
CCDS50344.1. [Q3U8K7-2]
CCDS50345.1. [Q3U8K7-4]
RefSeqiNP_001161356.1. NM_001167884.1. [Q3U8K7-4]
NP_001161357.1. NM_001167885.1. [Q3U8K7-1]
NP_001161358.1. NM_001167886.1. [Q3U8K7-3]
NP_001161359.1. NM_001167887.1. [Q3U8K7-1]
NP_001161360.1. NM_001167888.1. [Q3U8K7-4]
NP_001161361.1. NM_001167889.1. [Q3U8K7-2]
NP_659120.3. NM_144871.4. [Q3U8K7-3]
XP_006531790.1. XM_006531727.3. [Q3U8K7-1]
XP_006531791.1. XM_006531728.3. [Q3U8K7-1]
XP_006531794.1. XM_006531731.2. [Q3U8K7-5]
XP_011246925.1. XM_011248623.2. [Q3U8K7-1]
XP_011246926.1. XM_011248624.2. [Q3U8K7-3]
XP_017173632.1. XM_017318143.1. [Q3U8K7-1]
UniGeneiMm.278578.

Genome annotation databases

EnsembliENSMUST00000005518; ENSMUSP00000005518; ENSMUSG00000045098. [Q3U8K7-4]
ENSMUST00000052699; ENSMUSP00000060162; ENSMUSG00000045098. [Q3U8K7-2]
ENSMUST00000113968; ENSMUSP00000109601; ENSMUSG00000045098. [Q3U8K7-4]
ENSMUST00000113970; ENSMUSP00000109603; ENSMUSG00000045098. [Q3U8K7-2]
ENSMUST00000113972; ENSMUSP00000109605; ENSMUSG00000045098. [Q3U8K7-1]
ENSMUST00000113973; ENSMUSP00000109606; ENSMUSG00000045098. [Q3U8K7-1]
ENSMUST00000113974; ENSMUSP00000109607; ENSMUSG00000045098. [Q3U8K7-3]
ENSMUST00000113977; ENSMUSP00000109610; ENSMUSG00000045098. [Q3U8K7-3]
ENSMUST00000176262; ENSMUSP00000135563; ENSMUSG00000045098. [Q3U8K7-3]
GeneIDi225888.
KEGGimmu:225888.
UCSCiuc008fww.2. mouse. [Q3U8K7-4]
uc008fwz.2. mouse. [Q3U8K7-2]
uc008fxa.2. mouse. [Q3U8K7-1]
uc008fxc.2. mouse. [Q3U8K7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY555192 mRNA. Translation: AAT00539.1. Different initiation.
AK036880 mRNA. Translation: BAC29617.1.
AK054093 mRNA. Translation: BAC35652.1.
AK082660 mRNA. Translation: BAC38565.1.
AK083227 mRNA. Translation: BAC38817.1.
AK087267 mRNA. Translation: BAC39834.1. Different initiation.
AK139255 mRNA. Translation: BAE23934.1. Sequence problems.
AK152179 mRNA. Translation: BAE31010.1.
BC011214 mRNA. Translation: AAH11214.1. Different initiation.
BC075709 mRNA. Translation: AAH75709.1.
CCDSiCCDS29399.1. [Q3U8K7-3]
CCDS50343.1. [Q3U8K7-1]
CCDS50344.1. [Q3U8K7-2]
CCDS50345.1. [Q3U8K7-4]
RefSeqiNP_001161356.1. NM_001167884.1. [Q3U8K7-4]
NP_001161357.1. NM_001167885.1. [Q3U8K7-1]
NP_001161358.1. NM_001167886.1. [Q3U8K7-3]
NP_001161359.1. NM_001167887.1. [Q3U8K7-1]
NP_001161360.1. NM_001167888.1. [Q3U8K7-4]
NP_001161361.1. NM_001167889.1. [Q3U8K7-2]
NP_659120.3. NM_144871.4. [Q3U8K7-3]
XP_006531790.1. XM_006531727.3. [Q3U8K7-1]
XP_006531791.1. XM_006531728.3. [Q3U8K7-1]
XP_006531794.1. XM_006531731.2. [Q3U8K7-5]
XP_011246925.1. XM_011248623.2. [Q3U8K7-1]
XP_011246926.1. XM_011248624.2. [Q3U8K7-3]
XP_017173632.1. XM_017318143.1. [Q3U8K7-1]
UniGeneiMm.278578.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BUPX-ray2.17A/B70-336[»]
ProteinModelPortaliQ3U8K7.
SMRiQ3U8K7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230441. 3 interactors.
STRINGi10090.ENSMUSP00000109605.

PTM databases

iPTMnetiQ3U8K7.
PhosphoSitePlusiQ3U8K7.

Proteomic databases

MaxQBiQ3U8K7.
PaxDbiQ3U8K7.
PeptideAtlasiQ3U8K7.
PRIDEiQ3U8K7.

Protocols and materials databases

DNASUi225888.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005518; ENSMUSP00000005518; ENSMUSG00000045098. [Q3U8K7-4]
ENSMUST00000052699; ENSMUSP00000060162; ENSMUSG00000045098. [Q3U8K7-2]
ENSMUST00000113968; ENSMUSP00000109601; ENSMUSG00000045098. [Q3U8K7-4]
ENSMUST00000113970; ENSMUSP00000109603; ENSMUSG00000045098. [Q3U8K7-2]
ENSMUST00000113972; ENSMUSP00000109605; ENSMUSG00000045098. [Q3U8K7-1]
ENSMUST00000113973; ENSMUSP00000109606; ENSMUSG00000045098. [Q3U8K7-1]
ENSMUST00000113974; ENSMUSP00000109607; ENSMUSG00000045098. [Q3U8K7-3]
ENSMUST00000113977; ENSMUSP00000109610; ENSMUSG00000045098. [Q3U8K7-3]
ENSMUST00000176262; ENSMUSP00000135563; ENSMUSG00000045098. [Q3U8K7-3]
GeneIDi225888.
KEGGimmu:225888.
UCSCiuc008fww.2. mouse. [Q3U8K7-4]
uc008fwz.2. mouse. [Q3U8K7-2]
uc008fxa.2. mouse. [Q3U8K7-1]
uc008fxc.2. mouse. [Q3U8K7-3]

Organism-specific databases

CTDi225888.
MGIiMGI:2444557. Suv420h1.

Phylogenomic databases

eggNOGiKOG2589. Eukaryota.
ENOG410XPH8. LUCA.
GeneTreeiENSGT00520000055640.
HOVERGENiHBG105761.
InParanoidiQ3U8K7.
KOiK11429.
OMAiSGCLTRH.
OrthoDBiEOG091G0IUY.
PhylomeDBiQ3U8K7.
TreeFamiTF106433.

Enzyme and pathway databases

ReactomeiR-MMU-3214841. PKMTs methylate histone lysines.

Miscellaneous databases

ChiTaRSiSuv420h1. mouse.
PROiQ3U8K7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045098.
ExpressionAtlasiQ3U8K7. baseline and differential.
GenevisibleiQ3U8K7. MM.

Family and domain databases

InterProiIPR025790. Hist-Lys_N-MTase_Suvar4-20.
IPR001214. SET_dom.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00317. SET. 1 hit.
[Graphical view]
PROSITEiPS51570. SAM_MT43_SUVAR420_2. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKMT5B_MOUSE
AccessioniPrimary (citable) accession number: Q3U8K7
Secondary accession number(s): Q3UTP6
, Q6DI74, Q6Q784, Q8BU67, Q8BUN0, Q8BUT7, Q8BW73, Q8BZ24, Q91X81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: April 3, 2007
Last modified: November 30, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.