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Protein

Extended synaptotagmin-1

Gene

Esyt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi334Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi335Calcium 1By similarity1
Metal bindingi335Calcium 2By similarity1
Metal bindingi347Calcium 2By similarity1
Metal bindingi394Calcium 1By similarity1
Metal bindingi394Calcium 2By similarity1
Metal bindingi396Calcium 1By similarity1
Metal bindingi396Calcium 2By similarity1
Metal bindingi396Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi398Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi400Calcium 3By similarity1
Metal bindingi401Calcium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Lipid transport, Transport

Keywords - Ligandi

Calcium, Lipid-binding, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Extended synaptotagmin-1
Short name:
E-Syt1
Alternative name(s):
Membrane-bound C2 domain-containing protein
Gene namesi
Name:Esyt1
Synonyms:Fam62a, Mbc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1344426. Esyt1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28CytoplasmicSequence analysisAdd BLAST28
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Topological domaini50 – 52LumenalSequence analysis3
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 1092CytoplasmicSequence analysisAdd BLAST1019

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi314S → A: Abolished phosphorylation by CDK5. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3621032.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002343451 – 1092Extended synaptotagmin-1Add BLAST1092

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei314Phosphoserine; by CDK51 Publication1
Modified residuei804N6-acetyllysineBy similarity1
Modified residuei807PhosphoserineBy similarity1
Modified residuei937PhosphoserineBy similarity1
Modified residuei951PhosphoserineCombined sources1
Modified residuei997PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on Ser residues in insulin-treated adipocytes (in vitro); this promotes interaction with SLC2A4.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3U7R1.
MaxQBiQ3U7R1.
PaxDbiQ3U7R1.
PeptideAtlasiQ3U7R1.
PRIDEiQ3U7R1.

PTM databases

iPTMnetiQ3U7R1.
PhosphoSitePlusiQ3U7R1.
SwissPalmiQ3U7R1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025366.
CleanExiMM_MBC2.
GenevisibleiQ3U7R1. MM.

Interactioni

Subunit structurei

Interacts with ESYT2 and ESYT3 (By similarity). Interacts (phosphorylated form) with SLC2A4.By similarity1 Publication

Protein-protein interaction databases

BioGridi204808. 1 interactor.
DIPiDIP-48746N.
IntActiQ3U7R1. 1 interactor.
MINTiMINT-2633603.
STRINGi10090.ENSMUSP00000026427.

Structurei

3D structure databases

ProteinModelPortaliQ3U7R1.
SMRiQ3U7R1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini306 – 407C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini455 – 548C2 2PROSITE-ProRule annotationAdd BLAST94
Domaini621 – 722C2 3PROSITE-ProRule annotationAdd BLAST102
Domaini772 – 864C2 4PROSITE-ProRule annotationAdd BLAST93
Domaini950 – 1065C2 5PROSITE-ProRule annotationAdd BLAST116

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 303Glycerophospholipid-binding barrel-like domainBy similarityAdd BLAST175
Regioni1006 – 1013Required for phosphatidylinositol 4,5-bisphosphate-dependent location at the cell membraneBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi910 – 921Poly-SerAdd BLAST12

Domaini

Anchored to the endoplasmic reticulum membrane by a transmembrane hairpin structure; both N-terminus and C-terminus are cytoplasmic.By similarity
The C2 domains mediate lipid and calcium binding. The N-terminal C2 domain binds calcium ions and is important for calcium-dependent lipid binding and interaction with membranes. Two calcium ions are bound at a high-affinity site and a third calcium ion is bound with lower affinity. May bind up to four calcium ions. In contrast, the second C2 domain apparently does not bind calcium. The third C2 domain mediates interaction with membranes enriched in phosphatidylinositol 4,5-bisphosphate and is required for translocation to the cell membrane in response to increased cytosolic calcium levels (By similarity).By similarity
Contains a barrel-like domain that can bind various types of glycerophospholipids in its interior.By similarity

Sequence similaritiesi

Belongs to the extended synaptotagmin family.Curated
Contains 5 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1012. Eukaryota.
COG5038. LUCA.
GeneTreeiENSGT00550000074417.
HOGENOMiHOG000043080.
HOVERGENiHBG055795.
InParanoidiQ3U7R1.
OMAiSQHSGVE.
OrthoDBiEOG091G02X7.
PhylomeDBiQ3U7R1.
TreeFamiTF324255.

Family and domain databases

Gene3Di2.60.40.150. 5 hits.
InterProiIPR000008. C2_dom.
IPR031468. SMP_LBD.
[Graphical view]
PfamiPF00168. C2. 5 hits.
PF17047. SMP_LBD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00239. C2. 5 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 5 hits.
PROSITEiPS50004. C2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3U7R1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEHSPEEGAS PEPSGQPPAT DSTRDGGSGV PPAGPGAASE ALAVLTSFGR
60 70 80 90 100
RLLVLVPVYL AGAAGLSVGF VLFGLALYLG WRRVRDGKER SLRAARQLLD
110 120 130 140 150
DEERITAETL YMSHRELPAW VSFPDVEKAE WLNKIVAQVW PFLGQYMEKL
160 170 180 190 200
LAETVAPAVR GANPHLQTFT FTRVELGEKP LRIIGVKVHP SQRKDQILLD
210 220 230 240 250
LNVSYVGDVQ IDVEVKKYFC KAGVKGMQLH GVLRVILEPL TGDLPIVGAV
260 270 280 290 300
SMFFIKRPTL DINWTGMTNL LDIPGLSSLS DTMIMDSIAA FLVLPNRLLV
310 320 330 340 350
PLVPDLQDVA QLRSPLPRGI IRIHLLAARG LSSKDKYVKG LIEGKSDPYA
360 370 380 390 400
LVRVGTQTFC SRVIDEELNP HWGETYEVIV HEVPGQEIEV EVFDKDPDKD
410 420 430 440 450
DFLGRMKLDV GKVLQAGVLD NWYPLQGGQG QVHLRLEWLS LLPDAEKLDQ
460 470 480 490 500
VLQWNRGITS RPEPPSAAIL VVYLDRAQDL PLKKGNKEPN PMVQLSVQDV
510 520 530 540 550
TRESKATYST NSPVWEEAFR FFLQDPRSQE LDVQVKDDSR ALTLGALTLP
560 570 580 590 600
LARLLTASEL TLDQWFQLSS SGPNSRLYMK LVMRILYLDY SEIRFPTVPG
610 620 630 640 650
AQDWDRESLE TGSSVDAPPR PYHTTPNSHF GTENVLRIHV LEAQDLIAKD
660 670 680 690 700
RFLGGLVKGK SDPYVKLKVA GKSFRTHVVR EDLNPRWNEV FEVIVTSIPG
710 720 730 740 750
QELEIEVFDK DLDKDDFLGR YKVSLTTVLN SGFLDEWLTL EDVPSGRLHL
760 770 780 790 800
RLERLTPRPT AAELEEVLQV NSLIQTQKSS ELAAALLSVF LERAEDLPLR
810 820 830 840 850
KGTKPPSPYA TITVGETSHK TKTVSQSSAP VWEESASFLI RKPHAESLEL
860 870 880 890 900
QVRGEGTGTL GSVSLPLSEL LQEDQLCLDH WFALSGQGQV LMRAQLGILV
910 920 930 940 950
SQHSGVEAHS HSYSHSHSSS SLNDEPEALG GPTHPASPVL EVRHRLTHGD
960 970 980 990 1000
SPSEAPVGPL GQVKLTVWYH SDEQKLISII HSCRALRQNG RDLPDPYVSV
1010 1020 1030 1040 1050
LLLPDKNRST KRKTPQKKRT LNPEFNERFE WDLPLDGTLR RKLDVSVKSN
1060 1070 1080 1090
SSFMSREREL LGKVQLDLAE IDLSQGAAQW YDLMDDRDKG GS
Length:1,092
Mass (Da):121,554
Last modified:May 16, 2006 - v2
Checksum:i71A397EC52DE6DA9
GO
Isoform 2 (identifier: Q3U7R1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     852-893: VRGEGTGTLG...LSGQGQVLMR → ACFIHLLFLF...CGNLWATAHS
     894-1092: Missing.

Note: No experimental confirmation available.
Show »
Length:893
Mass (Da):99,673
Checksum:i956808B5CD6450E3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti385G → R in BAC32020 (PubMed:15489334).Curated1
Sequence conflicti392 – 394VFD → IQH in AAD10190 (PubMed:10350628).Curated3
Sequence conflicti829 – 841APVWE…SFLIR → GFGLEAKIRHEGG in AAD10189 (PubMed:10350628).CuratedAdd BLAST13

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018278852 – 893VRGEG…QVLMR → ACFIHLLFLFERGREQRTVF EFEVFGVNSFPPCGNLWATA HS in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_018279894 – 1092Missing in isoform 2. 1 PublicationAdd BLAST199

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008224 mRNA. Translation: BAB25542.1.
AK044656 mRNA. Translation: BAC32020.1.
AK152482 mRNA. Translation: BAE31254.1.
AK152554 mRNA. Translation: BAE31308.1.
BC011482 mRNA. Translation: AAH11482.1.
AF098633 mRNA. Translation: AAD10189.1.
AF098634 mRNA. Translation: AAD10190.1.
CCDSiCCDS24280.1. [Q3U7R1-1]
RefSeqiNP_035973.1. NM_011843.2. [Q3U7R1-1]
UniGeneiMm.66056.

Genome annotation databases

EnsembliENSMUST00000026427; ENSMUSP00000026427; ENSMUSG00000025366. [Q3U7R1-1]
GeneIDi23943.
KEGGimmu:23943.
UCSCiuc007hng.1. mouse. [Q3U7R1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008224 mRNA. Translation: BAB25542.1.
AK044656 mRNA. Translation: BAC32020.1.
AK152482 mRNA. Translation: BAE31254.1.
AK152554 mRNA. Translation: BAE31308.1.
BC011482 mRNA. Translation: AAH11482.1.
AF098633 mRNA. Translation: AAD10189.1.
AF098634 mRNA. Translation: AAD10190.1.
CCDSiCCDS24280.1. [Q3U7R1-1]
RefSeqiNP_035973.1. NM_011843.2. [Q3U7R1-1]
UniGeneiMm.66056.

3D structure databases

ProteinModelPortaliQ3U7R1.
SMRiQ3U7R1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204808. 1 interactor.
DIPiDIP-48746N.
IntActiQ3U7R1. 1 interactor.
MINTiMINT-2633603.
STRINGi10090.ENSMUSP00000026427.

Chemistry databases

ChEMBLiCHEMBL3621032.

PTM databases

iPTMnetiQ3U7R1.
PhosphoSitePlusiQ3U7R1.
SwissPalmiQ3U7R1.

Proteomic databases

EPDiQ3U7R1.
MaxQBiQ3U7R1.
PaxDbiQ3U7R1.
PeptideAtlasiQ3U7R1.
PRIDEiQ3U7R1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026427; ENSMUSP00000026427; ENSMUSG00000025366. [Q3U7R1-1]
GeneIDi23943.
KEGGimmu:23943.
UCSCiuc007hng.1. mouse. [Q3U7R1-1]

Organism-specific databases

CTDi23344.
MGIiMGI:1344426. Esyt1.

Phylogenomic databases

eggNOGiKOG1012. Eukaryota.
COG5038. LUCA.
GeneTreeiENSGT00550000074417.
HOGENOMiHOG000043080.
HOVERGENiHBG055795.
InParanoidiQ3U7R1.
OMAiSQHSGVE.
OrthoDBiEOG091G02X7.
PhylomeDBiQ3U7R1.
TreeFamiTF324255.

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.

Miscellaneous databases

ChiTaRSiEsyt1. mouse.
PROiQ3U7R1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025366.
CleanExiMM_MBC2.
GenevisibleiQ3U7R1. MM.

Family and domain databases

Gene3Di2.60.40.150. 5 hits.
InterProiIPR000008. C2_dom.
IPR031468. SMP_LBD.
[Graphical view]
PfamiPF00168. C2. 5 hits.
PF17047. SMP_LBD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
SMARTiSM00239. C2. 5 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 5 hits.
PROSITEiPS50004. C2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESYT1_MOUSE
AccessioniPrimary (citable) accession number: Q3U7R1
Secondary accession number(s): Q8C8R1
, Q91X62, Q9CVH0, Q9Z1X5, Q9Z1X6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 16, 2006
Last modified: November 30, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.