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Q3U6B2

- GP183_MOUSE

UniProt

Q3U6B2 - GP183_MOUSE

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Protein

G-protein coupled receptor 183

Gene

Gpr183

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha. Signals constitutively also via MAPK1/3 (ERK1/2).By similarity3 Publications

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW
  2. oxysterol binding Source: UniProtKB

GO - Biological processi

  1. G-protein coupled receptor signaling pathway Source: UniProtKB
  2. humoral immune response Source: UniProtKB
  3. mature B cell differentiation involved in immune response Source: UniProtKB
  4. positive regulation of B cell proliferation Source: UniProtKB
  5. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Adaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor 183
Alternative name(s):
Epstein-Barr virus-induced G-protein coupled receptor 2
Short name:
EBI2
Short name:
EBV-induced G-protein coupled receptor 2
Gene namesi
Name:Gpr183
Synonyms:Ebi2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:2442034. Gpr183.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727ExtracellularSequence AnalysisAdd
BLAST
Transmembranei28 – 5326Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini54 – 7320CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei74 – 9118Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini92 – 10110ExtracellularSequence Analysis
Transmembranei102 – 12322Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini124 – 14522CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei146 – 16419Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini165 – 18824ExtracellularSequence AnalysisAdd
BLAST
Transmembranei189 – 21123Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini212 – 23726CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei238 – 26124Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini262 – 28322ExtracellularSequence AnalysisAdd
BLAST
Transmembranei284 – 30825Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini309 – 35749CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display a reduction in the early antibody response to a T-dependent antigen. B-cells fail to move to the outer follicle at day 2 of activation, and instead are found in the follicle center. Mice have normal numbers of B and T cells and organized follicles and T-cell compartments are present.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357G-protein coupled receptor 183PRO_0000303231Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi4 – 41N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi100 ↔ 177PROSITE-ProRule annotation
Modified residuei324 – 3241Phosphoserine1 Publication
Modified residuei345 – 3451Phosphoserine1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ3U6B2.

PTM databases

PhosphoSiteiQ3U6B2.

Expressioni

Tissue specificityi

Expressed in mature B-cells and increases in expression early after activation, before being down-regulated in germinal center B-cells.1 Publication

Inductioni

Up-regulated during B-cell maturation in the bone marrow, and is expressed in mature recirculating B-cells in bone marrow, spleen and lymph nodes. Up-regulated in B-cells after BCR and CD40 engagement.1 Publication

Gene expression databases

BgeeiQ3U6B2.
GenevestigatoriQ3U6B2.

Structurei

3D structure databases

ProteinModelPortaliQ3U6B2.
SMRiQ3U6B2. Positions 20-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1309Interaction with G proteinsBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145860.
GeneTreeiENSGT00760000118784.
HOGENOMiHOG000043070.
HOVERGENiHBG101355.
InParanoidiQ3U6B2.
KOiK04305.
OMAiLVFYINT.
OrthoDBiEOG7VDXPF.
PhylomeDBiQ3U6B2.
TreeFamiTF350009.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3U6B2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANNFTTPLA TSHGNNCDLY AHHSTARVLM PLHYSLVFII GLVGNLLALV
60 70 80 90 100
VIVQNRKKIN STTLYSMNLV ISDILFTTAL PTRIAYYALG FDWRIGDALC
110 120 130 140 150
RVTALVFYIN TYAGVNFMTC LSIDRFFAVV HPLRYNKIKR IEYAKGVCLS
160 170 180 190 200
VWILVFAQTL PLLLTPMSKE EGDKTTCMEY PNFEGTASLP WILLGACLLG
210 220 230 240 250
YVLPITVILL CYSQICCKLF RTAKQNPLTE KSGVNKKALN TIILIIVVFI
260 270 280 290 300
LCFTPYHVAI IQHMIKMLCS PGALECGARH SFQISLHFTV CLMNFNCCMD
310 320 330 340 350
PFIYFFACKG YKRKVMKMLK RQVSVSISSA VRSAPEENSR EMTESQMMIH

SKASNGR
Length:357
Mass (Da):40,185
Last modified:October 11, 2005 - v1
Checksum:iD4B1C32C0C451993
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti163 – 1631L → F in AAH52868. (PubMed:15489334)Curated
Sequence conflicti239 – 2391L → P in BAE33542. (PubMed:16141072)Curated
Sequence conflicti338 – 3381N → S in AAH52868. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK138693 mRNA. Translation: BAE23750.1.
AK153216 mRNA. Translation: BAE31813.1.
AK156005 mRNA. Translation: BAE33542.1.
BC052868 mRNA. Translation: AAH52868.1.
AY255606 mRNA. Translation: AAO85118.1.
CCDSiCCDS27343.1.
RefSeqiNP_898852.2. NM_183031.2.
UniGeneiMm.265618.

Genome annotation databases

EnsembliENSMUST00000049872; ENSMUSP00000052404; ENSMUSG00000051212.
GeneIDi321019.
KEGGimmu:321019.
UCSCiuc007vaq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK138693 mRNA. Translation: BAE23750.1 .
AK153216 mRNA. Translation: BAE31813.1 .
AK156005 mRNA. Translation: BAE33542.1 .
BC052868 mRNA. Translation: AAH52868.1 .
AY255606 mRNA. Translation: AAO85118.1 .
CCDSi CCDS27343.1.
RefSeqi NP_898852.2. NM_183031.2.
UniGenei Mm.265618.

3D structure databases

ProteinModelPortali Q3U6B2.
SMRi Q3U6B2. Positions 20-319.
ModBasei Search...
MobiDBi Search...

Chemistry

GuidetoPHARMACOLOGYi 81.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei Q3U6B2.

Proteomic databases

PRIDEi Q3U6B2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000049872 ; ENSMUSP00000052404 ; ENSMUSG00000051212 .
GeneIDi 321019.
KEGGi mmu:321019.
UCSCi uc007vaq.1. mouse.

Organism-specific databases

CTDi 1880.
MGIi MGI:2442034. Gpr183.

Phylogenomic databases

eggNOGi NOG145860.
GeneTreei ENSGT00760000118784.
HOGENOMi HOG000043070.
HOVERGENi HBG101355.
InParanoidi Q3U6B2.
KOi K04305.
OMAi LVFYINT.
OrthoDBi EOG7VDXPF.
PhylomeDBi Q3U6B2.
TreeFami TF350009.

Miscellaneous databases

NextBioi 397884.
PROi Q3U6B2.
SOURCEi Search...

Gene expression databases

Bgeei Q3U6B2.
Genevestigatori Q3U6B2.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Spleen and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6NCr.
    Tissue: Hematopoietic stem cell.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 250-354.
  4. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324 AND SER-345, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Guidance of B cells by the orphan G protein-coupled receptor EBI2 shapes humoral immune responses."
    Gatto D., Paus D., Basten A., Mackay C.R., Brink R.
    Immunity 31:259-269(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "EBI2 mediates B cell segregation between the outer and centre follicle."
    Pereira J.P., Kelly L.M., Xu Y., Cyster J.G.
    Nature 460:1122-1126(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
  7. "Ligand modulation of the Epstein-Barr virus-induced seven-transmembrane receptor EBI2: identification of a potent and efficacious inverse agonist."
    Benned-Jensen T., Smethurst C., Holst P.J., Page K.R., Sauls H., Sivertsen B., Schwartz T.W., Blanchard A., Jepras R., Rosenkilde M.M.
    J. Biol. Chem. 286:29292-29302(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: IDENTIFICATION OF OXYSTEROLS AS ENDOGENOUS LIGANDS.
  9. Cited for: IDENTIFICATION OF OXYSTEROLS AS ENDOGENOUS LIGANDS.

Entry informationi

Entry nameiGP183_MOUSE
AccessioniPrimary (citable) accession number: Q3U6B2
Secondary accession number(s): Q3U1F6, Q7TMV7, Q80T40
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 11, 2005
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

GSK682753A (8-[(2E)-3-(4-chlorophenyl)prop-2-enoyl]-3-[(3,4-dichlorophenyl)methyl]-1-oxa-3,8-diazaspiro[4.5]decan-2-one), an inverse agonist, selectively inhibits the constitutive activity of GPR183 with high potency and efficacy.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3