Q3U5Q7 (CMPK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UMP-CMP kinase 2, mitochondrial
EC=2.7.4.14

Alternative name(s):
Thymidylate kinase LPS-inducible member
Short name=TYKi

Gene names

Name:	Cmpk2
Synonyms:	Tyki

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May participate in dUTP and dCTP synthesis in mitochondria. Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor By similarity.
Catalytic activity	ATP + (d)CMP = ADP + (d)CDP.
Subcellular location	Mitochondrion By similarity.
Induction	By lipopolysaccharides in macrophages and in primary microglia. Ref.3 Ref.4
Sequence similarities	Belongs to the thymidylate kinase family.
Sequence caution	The sequence AAA58770.1 differs from that shown. Reason: Sequence differs due to frameshifts, sequencing errors and other discrepancies. The sequence AAH27329.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH57565.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB23396.1 differs from that shown. Reason: Frameshift at position 74. The sequence BAE29625.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE29646.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE29803.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE29932.1 differs from that shown. Reason: Erroneous initiation. The sequence BAE31987.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Mitochondrion
Domain	Coiled coil Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to lipopolysaccharide Inferred from direct assay Ref.3. Source: MGI dTDP biosynthetic process Inferred from electronic annotation. Source: Compara dUDP biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrion Inferred from sequence orthology PubMed 17999954. Source: MGI nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UMP kinase activity Inferred from sequence orthology PubMed 17999954. Source: MGI cytidylate kinase activity Inferred from sequence orthology PubMed 17999954. Source: MGI thymidylate kinase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 73	73	Mitochondrion Potential
	Chain	74 – 447	374	UMP-CMP kinase 2, mitochondrial		PRO_0000331525
Regions
	Nucleotide binding	259 – 266	8	ATP Potential
	Coiled coil	380 – 412	33	Potential
Experimental info
	Sequence conflict	14	1	G → R in BAE29625. Ref.1
	Sequence conflict	183	1	R → K in BAE32020. Ref.1
	Sequence conflict	225	1	E → G in BAE32020. Ref.1
	Sequence conflict	317	1	E → G in BAE29625. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q3U5Q7 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 37CBACA251A40CFC	FASTA	447	50,036
	10 20 30 40 50 60 MALISRPRAP LWLGRLSRRL CGRHQACMGT MARPRRFTVE LPDCSLTHFV LGDATDHRDA 70 80 90 100 110 120 RLAELLGPPG RSYALCVPLA PGEGCGPRVQ AARVHHRLLQ QLRRGPLQRC QLSKLLGYGP 130 140 150 160 170 180 GDQAGEAQHG FLLRDPCDHP DTRRDLLQLL GSCQEAARPQ LAEFQADSQG LLWQRLWELQ 190 200 210 220 230 240 GDRQVQVDCA CVLPAQEPHL HPLLPDLLNS AVFQDRDAAR AVLEECTSFI PEARAVLDLV 250 260 270 280 290 300 DQCPKEVQKG KFQVIAIEGL DATGKTTLTQ SVSESLKAVL LQSPPPCISQ WRKIFDDEPT 310 320 330 340 350 360 IIRRAFYSLG NYLVASEIAK ESTNFPVIVD RYWHSTATYA IATEVSGGLQ YLPPAHHPVY 370 380 390 400 410 420 QWPGDLLKPD LVLLLTVNSE ERVRRLQGRG QEKTKEEAEL EANNVFRQKV EMTYQRMENP 430 440 SCHLVDASPS RETVLQKVLE LIQSSGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Bone marrow and Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and Czech II. Tissue: Mammary tumor.
[3]	"A unique member of the thymidylate kinase family that is induced during macrophage activation." Lee C.G.L., O'Brien W.E. J. Immunol. 154:6094-6102(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: BALB/c.
[4]	"The dynamics of the LPS triggered inflammatory response of murine microglia under different culture and in vivo conditions." Lund S., Christensen K.V., Hedtjarn M., Mortensen A.L., Hagberg H., Falsig J., Hasseldam H., Schrattenholz A., Poerzgen P., Leist M. J. Neuroimmunol. 180:71-87(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK004595 mRNA. Translation: BAB23396.1. Frameshift. AK153469 mRNA. Translation: BAE32020.1. AK150512 mRNA. Translation: BAE29625.1. Different initiation. AK150543 mRNA. Translation: BAE29646.1. Different initiation. AK150725 mRNA. Translation: BAE29803.1. Different initiation. AK150885 mRNA. Translation: BAE29932.1. Different initiation. AK153429 mRNA. Translation: BAE31987.1. Different initiation. BC027329 mRNA. Translation: AAH27329.1. Different initiation. BC057565 mRNA. Translation: AAH57565.1. Different initiation. L32973 mRNA. Translation: AAA58770.1. Sequence problems.
IPI	IPI00120113.
RefSeq	NP_065582.3. NM_020557.4.
UniGene	Mm.271839.
3D structure databases
ProteinModelPortal	Q3U5Q7.
ModBase	Search...
Proteomic databases
PaxDb	Q3U5Q7.
PRIDE	Q3U5Q7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020969; ENSMUSP00000020969; ENSMUSG00000020638.
GeneID	22169.
KEGG	mmu:22169.
UCSC	uc007nfj.1. mouse.
Organism-specific databases
CTD	129607.
MGI	MGI:99830. Cmpk2.
Phylogenomic databases
eggNOG	NOG259135.
GeneTree	ENSGT00640000091577.
HOGENOM	HOG000082499.
HOVERGEN	HBG088202.
InParanoid	Q3U5Q7.
KO	K13809.
OMA	HPVYQWP.
OrthoDB	EOG40K803.
Gene expression databases
Bgee	Q3U5Q7.
Genevestigator	Q3U5Q7.
Family and domain databases
InterPro	IPR014505. UMP-CMP_kinase_mit. [Graphical view]
PIRSF	PIRSF019736. dTMP_TKRP1. 1 hit.
PROSITE	PS01331. THYMIDYLATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	302112.
SOURCE	Search...

Entry information

Entry name

CMPK2_MOUSE

Accession

Primary (citable) accession number: Q3U5Q7
Secondary accession number(s): Q3UCI7 , Q5XKG5, Q62316, Q6PFG7, Q9DC34

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program