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Protein

UMP-CMP kinase 2, mitochondrial

Gene

Cmpk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May participate in dUTP and dCTP synthesis in mitochondria. Is able to phosphorylate dUMP, dCMP, CMP, UMP and monophosphates of the pyrimidine nucleoside analogs ddC, dFdC, araC, BVDU and FdUrd with ATP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity (By similarity).By similarity

Catalytic activityi

ATP + (d)CMP = ADP + (d)CDP.
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi259 – 2668ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cytidylate kinase activity Source: MGI
  3. nucleoside diphosphate kinase activity Source: UniProtKB
  4. thymidylate kinase activity Source: GO_Central
  5. UMP kinase activity Source: MGI
  6. uridylate kinase activity Source: GO_Central

GO - Biological processi

  1. cellular response to lipopolysaccharide Source: MGI
  2. dTDP biosynthetic process Source: GO_Central
  3. dTTP biosynthetic process Source: GO_Central
  4. dUDP biosynthetic process Source: GO_Central
  5. nucleoside diphosphate phosphorylation Source: UniProtKB
  6. nucleoside triphosphate biosynthetic process Source: UniProtKB
  7. nucleotide phosphorylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UMP-CMP kinase 2, mitochondrial (EC:2.7.4.14)
Alternative name(s):
Nucleoside-diphosphate kinase (EC:2.7.4.6)
Thymidylate kinase LPS-inducible member
Short name:
TYKi
Gene namesi
Name:Cmpk2
Synonyms:Tyki
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:99830. Cmpk2.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. cytosol Source: GO_Central
  2. mitochondrion Source: MGI
  3. nucleoplasm Source: MGI
  4. nucleus Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7373MitochondrionSequence AnalysisAdd
BLAST
Chaini74 – 447374UMP-CMP kinase 2, mitochondrialPRO_0000331525Add
BLAST

Proteomic databases

MaxQBiQ3U5Q7.
PaxDbiQ3U5Q7.
PRIDEiQ3U5Q7.

Expressioni

Inductioni

By lipopolysaccharides in macrophages and in primary microglia.2 Publications

Gene expression databases

BgeeiQ3U5Q7.
GenevestigatoriQ3U5Q7.

Structurei

3D structure databases

ProteinModelPortaliQ3U5Q7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili380 – 41233Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the thymidylate kinase family.Curated

Keywords - Domaini

Coiled coil, Transit peptide

Phylogenomic databases

eggNOGiNOG259135.
GeneTreeiENSGT00640000091577.
HOGENOMiHOG000082499.
HOVERGENiHBG088202.
InParanoidiQ3U5Q7.
KOiK13809.
OMAiHPVYQWP.
OrthoDBiEOG73NG3S.
PhylomeDBiQ3U5Q7.
TreeFamiTF328875.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR014505. UMP-CMP_kinase_mit.
[Graphical view]
PIRSFiPIRSF019736. dTMP_TKRP1. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3U5Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALISRPRAP LWLGRLSRRL CGRHQACMGT MARPRRFTVE LPDCSLTHFV
60 70 80 90 100
LGDATDHRDA RLAELLGPPG RSYALCVPLA PGEGCGPRVQ AARVHHRLLQ
110 120 130 140 150
QLRRGPLQRC QLSKLLGYGP GDQAGEAQHG FLLRDPCDHP DTRRDLLQLL
160 170 180 190 200
GSCQEAARPQ LAEFQADSQG LLWQRLWELQ GDRQVQVDCA CVLPAQEPHL
210 220 230 240 250
HPLLPDLLNS AVFQDRDAAR AVLEECTSFI PEARAVLDLV DQCPKEVQKG
260 270 280 290 300
KFQVIAIEGL DATGKTTLTQ SVSESLKAVL LQSPPPCISQ WRKIFDDEPT
310 320 330 340 350
IIRRAFYSLG NYLVASEIAK ESTNFPVIVD RYWHSTATYA IATEVSGGLQ
360 370 380 390 400
YLPPAHHPVY QWPGDLLKPD LVLLLTVNSE ERVRRLQGRG QEKTKEEAEL
410 420 430 440
EANNVFRQKV EMTYQRMENP SCHLVDASPS RETVLQKVLE LIQSSGR
Length:447
Mass (Da):50,036
Last modified:April 29, 2008 - v2
Checksum:i37CBACA251A40CFC
GO

Sequence cautioni

The sequence AAA58770.1 differs from that shown.Sequence differs due to frameshifts, sequencing errors and other discrepancies.Curated
The sequence AAH27329.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH57565.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB23396.1 differs from that shown. Reason: Frameshift at position 74. Curated
The sequence BAE29625.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE29646.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE29803.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE29932.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE31987.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141G → R in BAE29625 (PubMed:16141072).Curated
Sequence conflicti183 – 1831R → K in BAE32020 (PubMed:16141072).Curated
Sequence conflicti225 – 2251E → G in BAE32020 (PubMed:16141072).Curated
Sequence conflicti317 – 3171E → G in BAE29625 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004595 mRNA. Translation: BAB23396.1. Frameshift.
AK153469 mRNA. Translation: BAE32020.1.
AK150512 mRNA. Translation: BAE29625.1. Different initiation.
AK150543 mRNA. Translation: BAE29646.1. Different initiation.
AK150725 mRNA. Translation: BAE29803.1. Different initiation.
AK150885 mRNA. Translation: BAE29932.1. Different initiation.
AK153429 mRNA. Translation: BAE31987.1. Different initiation.
BC027329 mRNA. Translation: AAH27329.1. Different initiation.
BC057565 mRNA. Translation: AAH57565.1. Different initiation.
L32973 mRNA. Translation: AAA58770.1. Sequence problems.
CCDSiCCDS49039.1.
RefSeqiNP_065582.3. NM_020557.4.
UniGeneiMm.271839.

Genome annotation databases

EnsembliENSMUST00000020969; ENSMUSP00000020969; ENSMUSG00000020638.
GeneIDi22169.
KEGGimmu:22169.
UCSCiuc007nfj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004595 mRNA. Translation: BAB23396.1. Frameshift.
AK153469 mRNA. Translation: BAE32020.1.
AK150512 mRNA. Translation: BAE29625.1. Different initiation.
AK150543 mRNA. Translation: BAE29646.1. Different initiation.
AK150725 mRNA. Translation: BAE29803.1. Different initiation.
AK150885 mRNA. Translation: BAE29932.1. Different initiation.
AK153429 mRNA. Translation: BAE31987.1. Different initiation.
BC027329 mRNA. Translation: AAH27329.1. Different initiation.
BC057565 mRNA. Translation: AAH57565.1. Different initiation.
L32973 mRNA. Translation: AAA58770.1. Sequence problems.
CCDSiCCDS49039.1.
RefSeqiNP_065582.3. NM_020557.4.
UniGeneiMm.271839.

3D structure databases

ProteinModelPortaliQ3U5Q7.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiQ3U5Q7.
PaxDbiQ3U5Q7.
PRIDEiQ3U5Q7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020969; ENSMUSP00000020969; ENSMUSG00000020638.
GeneIDi22169.
KEGGimmu:22169.
UCSCiuc007nfj.1. mouse.

Organism-specific databases

CTDi129607.
MGIiMGI:99830. Cmpk2.

Phylogenomic databases

eggNOGiNOG259135.
GeneTreeiENSGT00640000091577.
HOGENOMiHOG000082499.
HOVERGENiHBG088202.
InParanoidiQ3U5Q7.
KOiK13809.
OMAiHPVYQWP.
OrthoDBiEOG73NG3S.
PhylomeDBiQ3U5Q7.
TreeFamiTF328875.

Miscellaneous databases

NextBioi302112.
PROiQ3U5Q7.
SOURCEiSearch...

Gene expression databases

BgeeiQ3U5Q7.
GenevestigatoriQ3U5Q7.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR014505. UMP-CMP_kinase_mit.
[Graphical view]
PIRSFiPIRSF019736. dTMP_TKRP1. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow and Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and Czech II.
    Tissue: Mammary tumor.
  3. "A unique member of the thymidylate kinase family that is induced during macrophage activation."
    Lee C.G.L., O'Brien W.E.
    J. Immunol. 154:6094-6102(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Strain: BALB/c.
  4. "The dynamics of the LPS triggered inflammatory response of murine microglia under different culture and in vivo conditions."
    Lund S., Christensen K.V., Hedtjarn M., Mortensen A.L., Hagberg H., Falsig J., Hasseldam H., Schrattenholz A., Poerzgen P., Leist M.
    J. Neuroimmunol. 180:71-87(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiCMPK2_MOUSE
AccessioniPrimary (citable) accession number: Q3U5Q7
Secondary accession number(s): Q3UCI7
, Q5XKG5, Q62316, Q6PFG7, Q9DC34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: March 4, 2015
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.