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Protein

Rho guanine nucleotide exchange factor 16

Gene

Arhgef16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 16
Alternative name(s):
Ephexin-4
Gene namesi
Name:Arhgef16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2446219. Arhgef16.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 713712Rho guanine nucleotide exchange factor 16PRO_0000233691Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei41 – 411PhosphoserineBy similarity
Modified residuei111 – 1111PhosphoserineCombined sources
Modified residuei212 – 2121PhosphoserineCombined sources
Modified residuei230 – 2301PhosphothreonineCombined sources
Modified residuei231 – 2311PhosphoserineCombined sources
Modified residuei234 – 2341PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3U5C8.
PaxDbiQ3U5C8.
PeptideAtlasiQ3U5C8.
PRIDEiQ3U5C8.

PTM databases

iPTMnetiQ3U5C8.
PhosphoSiteiQ3U5C8.

Expressioni

Gene expression databases

BgeeiQ3U5C8.
CleanExiMM_ARHGEF16.
ExpressionAtlasiQ3U5C8. baseline and differential.
GenevisibleiQ3U5C8. MM.

Interactioni

Subunit structurei

Interacts with ELMO2, EPHA2, RAC1 and RHOG; mediates activation of RAC1 by EPHA2. Interacts with TAX1BP3 (via PDZ domain) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ3U5C8. 1 interaction.
MINTiMINT-4119540.
STRINGi10090.ENSMUSP00000030898.

Structurei

3D structure databases

ProteinModelPortaliQ3U5C8.
SMRiQ3U5C8. Positions 241-619, 632-692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini288 – 472185DHPROSITE-ProRule annotationAdd
BLAST
Domaini505 – 624120PHPROSITE-ProRule annotationAdd
BLAST
Domaini633 – 69361SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni279 – 485207Required for RHOG activation and mediates interaction with EPHA2By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi711 – 7133PDZ-binding motif

Domaini

The PDZ-binding motif mediates interaction with TAX1BP3.

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3523. Eukaryota.
COG5422. LUCA.
GeneTreeiENSGT00550000074493.
HOGENOMiHOG000007536.
HOVERGENiHBG059167.
InParanoidiQ3U5C8.
OMAiQTEHHHL.
OrthoDBiEOG73NG2P.
TreeFamiTF316357.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3U5C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRHSDSSL DEKLLEYRFH AELRLDANGN PIPGLPMVRS SLKARANAAF
60 70 80 90 100
EPDASEPLPP PPSPEDEEPP RPIVLSTQSP AALKMGTQQL IPKSLAVASK
110 120 130 140 150
AKTKSPARHQ SFGAAVLSKE AARRNPQLFS APSFSLDDMD MDMVTTGNLR
160 170 180 190 200
RNLRNQSYRA AMKGPGPPSS KGDSVQLGPK LQALAEEAAQ PPSRYPAKNK
210 220 230 240 250
KTLGRKRAHK GSFKDDPQLY QEIRERGLNT SHESDDDILD EPSGPVGTQR
260 270 280 290 300
ADTTIVVKSY RPAQLTWSQL PEVLESGVLD TLSTEERKRQ EAIFEILTSE
310 320 330 340 350
FSYLHSLSIL VTEFLQSREL RATMTQTEHH HLFSNILDVM SASQKFFEAL
360 370 380 390 400
EQRHKAQVCV EDISDILEDH AQHHFHPYIA YCSNEVYQQR TLQKLSNSNA
410 420 430 440 450
AFRDVLKEIE KRPACGGLPM ISFLILPMQR VTRLPLLTDT LCLKTQGHPE
460 470 480 490 500
RYKAASQALK AISKLVKQCN EGAHKMERTE QIYTLNMQLD FGKVKSLPLI
510 520 530 540 550
SASRWLLKRG ELFLLEESSI FRKIASRPTC YLFLFNDVLV VTKKKSEESY
560 570 580 590 600
LVQDYAQLDH VQVRKLEPSE PLLPGGSSRS SSVPYPFQVN LLHNSEGRQE
610 620 630 640 650
QILLSSDSAS DRARWITALT YKERQWQGIT NKGELPQVEV TKAYFAKQAD
660 670 680 690 700
EITLQQADIV LVLQEEDGWL HGERLRDGET GWFPESFAHS ITSRVAVEGN
710
VRRMERLRVE TDV
Length:713
Mass (Da):80,381
Last modified:July 27, 2011 - v3
Checksum:i823AC537814C80F7
GO

Sequence cautioni

The sequence BAE32151.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti538 – 5381V → L in BAE32151 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK153708 mRNA. Translation: BAE32151.1. Different initiation.
AL627123 Genomic DNA. Translation: CAM21849.1.
BC137847 mRNA. Translation: AAI37848.1.
BC144970 mRNA. Translation: AAI44971.1.
BC020030 mRNA. Translation: AAH20030.1.
BC095966 mRNA. Translation: AAH95966.1.
CCDSiCCDS51399.1.
RefSeqiNP_001106215.1. NM_001112744.1.
XP_006538892.1. XM_006538829.2.
XP_011248544.1. XM_011250242.1.
XP_011248545.1. XM_011250243.1.
UniGeneiMm.38481.

Genome annotation databases

EnsembliENSMUST00000030898; ENSMUSP00000030898; ENSMUSG00000029032.
ENSMUST00000169623; ENSMUSP00000126296; ENSMUSG00000029032.
GeneIDi230972.
KEGGimmu:230972.
UCSCiuc008wbr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK153708 mRNA. Translation: BAE32151.1. Different initiation.
AL627123 Genomic DNA. Translation: CAM21849.1.
BC137847 mRNA. Translation: AAI37848.1.
BC144970 mRNA. Translation: AAI44971.1.
BC020030 mRNA. Translation: AAH20030.1.
BC095966 mRNA. Translation: AAH95966.1.
CCDSiCCDS51399.1.
RefSeqiNP_001106215.1. NM_001112744.1.
XP_006538892.1. XM_006538829.2.
XP_011248544.1. XM_011250242.1.
XP_011248545.1. XM_011250243.1.
UniGeneiMm.38481.

3D structure databases

ProteinModelPortaliQ3U5C8.
SMRiQ3U5C8. Positions 241-619, 632-692.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3U5C8. 1 interaction.
MINTiMINT-4119540.
STRINGi10090.ENSMUSP00000030898.

PTM databases

iPTMnetiQ3U5C8.
PhosphoSiteiQ3U5C8.

Proteomic databases

MaxQBiQ3U5C8.
PaxDbiQ3U5C8.
PeptideAtlasiQ3U5C8.
PRIDEiQ3U5C8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030898; ENSMUSP00000030898; ENSMUSG00000029032.
ENSMUST00000169623; ENSMUSP00000126296; ENSMUSG00000029032.
GeneIDi230972.
KEGGimmu:230972.
UCSCiuc008wbr.2. mouse.

Organism-specific databases

CTDi27237.
MGIiMGI:2446219. Arhgef16.

Phylogenomic databases

eggNOGiKOG3523. Eukaryota.
COG5422. LUCA.
GeneTreeiENSGT00550000074493.
HOGENOMiHOG000007536.
HOVERGENiHBG059167.
InParanoidiQ3U5C8.
OMAiQTEHHHL.
OrthoDBiEOG73NG2P.
TreeFamiTF316357.

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.

Miscellaneous databases

PROiQ3U5C8.
SOURCEiSearch...

Gene expression databases

BgeeiQ3U5C8.
CleanExiMM_ARHGEF16.
ExpressionAtlasiQ3U5C8. baseline and differential.
GenevisibleiQ3U5C8. MM.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.
  4. Cited for: IDENTIFICATION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111; SER-212; THR-230; SER-231 AND SER-234, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Liver, Lung and Pancreas.

Entry informationi

Entry nameiARHGG_MOUSE
AccessioniPrimary (citable) accession number: Q3U5C8
Secondary accession number(s): B1ASH6, Q501M8, Q8VCE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.