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Protein

Rho guanine nucleotide exchange factor 16

Gene

Arhgef16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanyl-nucleotide exchange factor of the RHOG GTPase stimulating the exchange of RHOG-associated GDP for GTP. May play a role in chemotactic cell migration by mediating the activation of RAC1 by EPHA2. May also activate CDC42 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 16
Alternative name(s):
Ephexin-4
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2446219 Arhgef16

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002336911 – 713Rho guanine nucleotide exchange factor 16Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineBy similarity1
Modified residuei41PhosphoserineBy similarity1
Modified residuei111PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei230PhosphothreonineCombined sources1
Modified residuei231PhosphoserineCombined sources1
Modified residuei234PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3U5C8
PaxDbiQ3U5C8
PeptideAtlasiQ3U5C8
PRIDEiQ3U5C8

PTM databases

iPTMnetiQ3U5C8
PhosphoSitePlusiQ3U5C8

Expressioni

Gene expression databases

BgeeiENSMUSG00000029032
CleanExiMM_ARHGEF16
ExpressionAtlasiQ3U5C8 baseline and differential
GenevisibleiQ3U5C8 MM

Interactioni

Subunit structurei

Interacts with ELMO2, EPHA2, RAC1 and RHOG; mediates activation of RAC1 by EPHA2. Interacts with TAX1BP3 (via PDZ domain) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ3U5C8, 1 interactor
MINTiQ3U5C8
STRINGi10090.ENSMUSP00000030898

Structurei

3D structure databases

ProteinModelPortaliQ3U5C8
SMRiQ3U5C8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini288 – 472DHPROSITE-ProRule annotationAdd BLAST185
Domaini505 – 624PHPROSITE-ProRule annotationAdd BLAST120
Domaini633 – 693SH3PROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni279 – 485Required for RHOG activation and mediates interaction with EPHA2By similarityAdd BLAST207

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi711 – 713PDZ-binding motif3

Domaini

The PDZ-binding motif mediates interaction with TAX1BP3.

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3523 Eukaryota
COG5422 LUCA
GeneTreeiENSGT00550000074493
HOGENOMiHOG000007536
HOVERGENiHBG059167
InParanoidiQ3U5C8
KOiK20688
OMAiKTPARHQ
OrthoDBiEOG091G01WI
TreeFamiTF316357

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
cd11938 SH3_ARHGEF16_26, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035797 ARHGEF16/ARHGEF26_SH3
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
PF14604 SH3_9, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q3U5C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQRHSDSSL DEKLLEYRFH AELRLDANGN PIPGLPMVRS SLKARANAAF
60 70 80 90 100
EPDASEPLPP PPSPEDEEPP RPIVLSTQSP AALKMGTQQL IPKSLAVASK
110 120 130 140 150
AKTKSPARHQ SFGAAVLSKE AARRNPQLFS APSFSLDDMD MDMVTTGNLR
160 170 180 190 200
RNLRNQSYRA AMKGPGPPSS KGDSVQLGPK LQALAEEAAQ PPSRYPAKNK
210 220 230 240 250
KTLGRKRAHK GSFKDDPQLY QEIRERGLNT SHESDDDILD EPSGPVGTQR
260 270 280 290 300
ADTTIVVKSY RPAQLTWSQL PEVLESGVLD TLSTEERKRQ EAIFEILTSE
310 320 330 340 350
FSYLHSLSIL VTEFLQSREL RATMTQTEHH HLFSNILDVM SASQKFFEAL
360 370 380 390 400
EQRHKAQVCV EDISDILEDH AQHHFHPYIA YCSNEVYQQR TLQKLSNSNA
410 420 430 440 450
AFRDVLKEIE KRPACGGLPM ISFLILPMQR VTRLPLLTDT LCLKTQGHPE
460 470 480 490 500
RYKAASQALK AISKLVKQCN EGAHKMERTE QIYTLNMQLD FGKVKSLPLI
510 520 530 540 550
SASRWLLKRG ELFLLEESSI FRKIASRPTC YLFLFNDVLV VTKKKSEESY
560 570 580 590 600
LVQDYAQLDH VQVRKLEPSE PLLPGGSSRS SSVPYPFQVN LLHNSEGRQE
610 620 630 640 650
QILLSSDSAS DRARWITALT YKERQWQGIT NKGELPQVEV TKAYFAKQAD
660 670 680 690 700
EITLQQADIV LVLQEEDGWL HGERLRDGET GWFPESFAHS ITSRVAVEGN
710
VRRMERLRVE TDV
Length:713
Mass (Da):80,381
Last modified:July 27, 2011 - v3
Checksum:i823AC537814C80F7
GO

Sequence cautioni

The sequence BAE32151 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti538V → L in BAE32151 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK153708 mRNA Translation: BAE32151.1 Different initiation.
AL627123 Genomic DNA No translation available.
BC137847 mRNA Translation: AAI37848.1
BC144970 mRNA Translation: AAI44971.1
BC020030 mRNA Translation: AAH20030.1
BC095966 mRNA Translation: AAH95966.1
CCDSiCCDS51399.1
RefSeqiNP_001106215.1, NM_001112744.1
XP_006538892.1, XM_006538829.3
XP_011248544.1, XM_011250242.2
UniGeneiMm.38481

Genome annotation databases

EnsembliENSMUST00000030898; ENSMUSP00000030898; ENSMUSG00000029032
ENSMUST00000169623; ENSMUSP00000126296; ENSMUSG00000029032
GeneIDi230972
KEGGimmu:230972
UCSCiuc008wbr.2 mouse

Similar proteinsi

Entry informationi

Entry nameiARHGG_MOUSE
AccessioniPrimary (citable) accession number: Q3U5C8
Secondary accession number(s): B1ASH6, Q501M8, Q8VCE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 108 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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