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Q3U4W8 (Q3U4W8_MOUSE) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase RuleBase RU004435

EC=3.4.19.12 RuleBase RU004435
Gene names
Name:Usp5 Ensembl ENSMUSP00000114000 MGI 1347343
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). SAAS SAAS001607 RuleBase RU004435

Sequence similarities

Belongs to the peptidase C19 family. RuleBase RU004434

Contains USP domain. SAAS SAAS001607

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region221 – 2244Substrate binding By similarity PIRSR PIRSR016308-2

Sites

Active site3351Nucleophile By similarity PIRSR PIRSR016308-1
Active site7951Proton acceptor By similarity PIRSR PIRSR016308-1
Binding site2091Substrate By similarity PIRSR PIRSR016308-2
Binding site2591Substrate By similarity PIRSR PIRSR016308-2
Binding site2611Substrate; via carbonyl oxygen By similarity PIRSR PIRSR016308-2
Binding site2641Substrate By similarity PIRSR PIRSR016308-2

Sequences

Sequence LengthMass (Da)Tools
Q3U4W8 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: E7E920647E8CB9AE

FASTA83593,355
        10         20         30         40         50         60 
MAELSEEALL SVLPTIRVPK AGDRVHKDEC AFSFDTPESE GGLYICMNTF LGFGKQYVER 

        70         80         90        100        110        120 
HFNKTGQRVY LHLRRTRRPK EEDTSAGTGD PPRKKPTRLA IGVEGGFDLT EDKFEFDEDV 

       130        140        150        160        170        180 
KIVILPDYLE IARDGLGGLP DIVRDRVTSA VEALLSADSA SRKQEVQAWD GEVRQVSKHA 

       190        200        210        220        230        240 
FNLKQLDNPA RIPPCGWKCS KCDMRENLWL NLTDGSILCG RRYFDGSGGN NHAVEHYRET 

       250        260        270        280        290        300 
GYPLAVKLGT ITPDGADVYS YDEDDMVLDP SLAEHLSHFG IDMLKMQKTD KTMTELEIDM 

       310        320        330        340        350        360 
NQRIGEWELI QESGVPLKPL FGPGYTGIRN LGNSCYLNSV VQVLFSIPDF QRKYVDKLEK 

       370        380        390        400        410        420 
IFQNAPTDPT QDFSTQVAKL GHGLLSGEYS KPALESGDGE QVPEQKEVQD GIAPRMFKAL 

       430        440        450        460        470        480 
IGKGHPEFST NRQQDAQEFF LHLINMVERN CRSSENPNEV FRFLVEEKIK CLATEKVKYT 

       490        500        510        520        530        540 
QRVDYIMQLP VPMDAALNKE ELLEYEEKKR QAEEEKVPLP ELVRAQVPFS SCLEAYGAPE 

       550        560        570        580        590        600 
QVDDFWSTAL QAKSVAVKTT RFASFPDYLV IQIKKFTFGL DWVPKKLDVS IEMPEELDIS 

       610        620        630        640        650        660 
QLRGTGLQPG EEELPDIAPP LVTPDEPKAP MLDESVIIQL VEMGFPMDAC RKAVYYTGNS 

       670        680        690        700        710        720 
GAEAAMNWVM SHMDDPDFAN PLILPGSSGP GSTSAAADPP PEDCVTTIVS MGFSRDQALK 

       730        740        750        760        770        780 
ALRATNNSLE RAVDWIFSHI DDLDAEAAMD ISEGRSAAES ISESVPVGPK VRDGPGKYQL 

       790        800        810        820        830 
FAFISHMGTS TMCGHYVCHI KKEGRWVIYN DQKVCASEKP PKDLGYIYFY QRVVS 

« Hide

References

« Hide 'large scale' references
[1]"High-efficiency full-length cDNA cloning."
Carninci P., Hayashizaki Y.
Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NOD EMBL BAE32312.1.
Tissue: Thymus EMBL BAE32312.1.
[2]"Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NOD EMBL BAE32312.1.
Tissue: Thymus EMBL BAE32312.1.
[3]"RIKEN integrated sequence analysis (RISA) system--384-format sequencing pipeline with 384 multicapillary sequencer."
Shibata K., Itoh M., Aizawa K., Nagaoka S., Sasaki N., Carninci P., Konno H., Akiyama J., Nishi K., Kitsunai T., Tashiro H., Itoh M., Sumi N., Ishii Y., Nakamura S., Hazama M., Nishine T., Harada A. expand/collapse author list , Yamamoto R., Matsumoto H., Sakaguchi S., Ikegami T., Kashiwagi K., Fujiwake S., Inoue K., Togawa Y., Izawa M., Ohara E., Watahiki M., Yoneda Y., Ishikawa T., Ozawa K., Tanaka T., Matsuura S., Kawai J., Okazaki Y., Muramatsu M., Inoue Y., Kira A., Hayashizaki Y.
Genome Res. 10:1757-1771(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NOD EMBL BAE32312.1.
Tissue: Thymus EMBL BAE32312.1.
[4]"Functional annotation of a full-length mouse cDNA collection."
The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium
Nature 409:685-690(2001)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NOD EMBL BAE32312.1.
Tissue: Thymus EMBL BAE32312.1.
[5]"Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
Nature 420:563-573(2002)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NOD EMBL BAE32312.1.
Tissue: Thymus EMBL BAE32312.1.
[6]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]Arakawa T., Carninci P., Fukuda S., Hashizume W., Hayashida K., Hori F., Iida J., Imamura K., Imotani K., Itoh M., Kanagawa S., Kawai J., Kojima M., Konno H., Murata M., Nakamura M., Ninomiya N., Nishiyori H. expand/collapse author list , Nomura K., Ohno M., Sakazume N., Sano H., Sasaki D., Shibata K., Shiraki T., Tagami M., Tagami Y., Waki K., Watahiki A., Muramatsu M., Hayashizaki Y.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NOD EMBL BAE32312.1.
Tissue: Thymus EMBL BAE32312.1.
[8]"The Transcriptional Landscape of the Mammalian Genome."
The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
Science 309:1559-1563(2005)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NOD EMBL BAE32312.1.
Tissue: Thymus EMBL BAE32312.1.
[9]"Antisense Transcription in the Mammalian Transcriptome."
RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium
Science 309:1564-1566(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: NOD EMBL BAE32312.1.
Tissue: Thymus EMBL BAE32312.1.
[10]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J Ensembl ENSMUSP00000114000.
[11]Ensembl
Submitted (FEB-2012) to UniProtKB
Cited for: IDENTIFICATION.
Strain: C57BL/6J Ensembl ENSMUSP00000114000.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC142254 Genomic DNA. No translation available.
AK154008 mRNA. Translation: BAE32312.1.
RefSeqXP_006505978.1. XM_006505915.1.
UniGeneMm.3571.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000122110; ENSMUSP00000114000; ENSMUSG00000038429.
GeneID22225.
KEGGmmu:22225.
UCSCuc009dsb.1. mouse.

Organism-specific databases

CTD8078.
MGIMGI:1347343. Usp5.

Phylogenomic databases

GeneTreeENSGT00390000000874.
HOGENOMHOG000162311.
HOVERGENHBG002833.
KOK11836.

Gene expression databases

GenevestigatorQ3U4W8.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000449. UBA/Ts_N.
IPR016652. Ubiquitinyl_hydrolase.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00627. UBA. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PIRSFPIRSF016308. UBP. 1 hit.
SMARTSM00165. UBA. 2 hits.
SM00290. ZnF_UBP. 1 hit.
[Graphical view]
SUPFAMSSF46934. SSF46934. 1 hit.
PROSITEPS50030. UBA. 2 hits.
PS00972. UCH_2_1. 1 hit.
PS00973. UCH_2_2. 1 hit.
PS50235. UCH_2_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUSP5. mouse.
SOURCESearch...

Entry information

Entry nameQ3U4W8_MOUSE
AccessionPrimary (citable) accession number: Q3U4W8
Entry history
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)