Q3U492 (KCP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kielin/chordin-like protein

Alternative name(s):
Cysteine-rich BMP regulator 2
Cysteine-rich motor neuron 2 protein
Short name=CRIM-2
Kielin/chordin-like protein 1
Short name=KCP-1

Gene names

Name:	Kcp
Synonyms:	Crim2, Kcp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enhances bone morphogenetic protein (BMP) signaling in a paracrine manner. In contrast, it inhibits both the activin-A and TGFB1-mediated signaling pathways. Ref.1
Subunit structure	Interacts with BMP7 and, by doing so, enhances binding to the type I receptors that contains cytoplasmic serine-/threonine protein kinase domains. Also able to interact with activin-A and TGFB1. Ref.1 Ref.4
Subcellular location	Secreted Ref.1.
Tissue specificity	Weakly expressed in embryonic kidney and brain. Not expressed in adult tissues and several cell lines. Ref.1
Developmental stage	Prominently expressed in two areas, the limb buds and the developing kidney, with diffuse staining in the central nervous system. At E9.5 the limb bud mesenchyme is positive. Expression in the kidney region could be detected as early as E9 in the intermediate mesoderm. By E10, the mesonephric tubules and nephric ducts are clearly positive. At later stages, high levels are localized to the developing tubules. At E18.5, it is localized to more mature renal tubules located in the developing cortex with little expression detected in the nephrogenic zone. Ref.1
Induction	By renal stresses. Ref.1
Sequence similarities	Contains 1 TIL (trypsin inhibitory-like) domain. Contains 18 VWFC domains. Contains 1 VWFD domain.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of BMP signaling pathway Inferred from direct assay Ref.1. Source: MGI
Cellular_component	extracellular space Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3U492-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3U492-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1250-1255: SVHVTN → RWTSTQ
     1256-1550: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 1550	1529	Kielin/chordin-like protein		PRO_0000318587
Regions
	Domain	134 – 191	58	VWFC 1
	Domain	192 – 251	60	VWFC 2
	Domain	253 – 312	60	VWFC 3
	Domain	313 – 370	58	VWFC 4
	Domain	371 – 425	55	VWFC 5
	Domain	426 – 485	60	VWFC 6
	Domain	486 – 544	59	VWFC 7
	Domain	545 – 602	58	VWFC 8
	Domain	603 – 661	59	VWFC 9
	Domain	665 – 724	60	VWFC 10
	Domain	725 – 781	57	VWFC 11
	Domain	782 – 840	59	VWFC 12
	Domain	841 – 902	62	VWFC 13
	Domain	903 – 958	56	VWFC 14
	Domain	959 – 1016	58	VWFC 15
	Domain	1017 – 1084	68	VWFC 16
	Domain	1085 – 1144	60	VWFC 17
	Domain	1148 – 1208	61	VWFC 18
	Domain	1213 – 1425	213	VWFD
	Domain	1489 – 1542	54	TIL
	Coiled coil	60 – 92	33	Potential
Amino acid modifications
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Disulfide bond	1236 ↔ 1244		By similarity
Natural variations
	Alternative sequence	1250 – 1255	6	SVHVTN → RWTSTQ in isoform 2.		VSP_031232
	Alternative sequence	1256 – 1550	295	Missing in isoform 2.		VSP_031233
Experimental info
	Sequence conflict	385	1	F → L in AAX77677. Ref.1
	Sequence conflict	829	1	L → F in BAE32541. Ref.2
	Sequence conflict	1045	1	Missing in AAX77677. Ref.1
	Sequence conflict	1147	1	T → N in BAE32541. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 6E6630EDA239D744	FASTA	1,550	166,628
	10 20 30 40 50 60 MAGARAALLP LLLHLGSLAL AARGGEVSRE QPRLADAISQ QQAPSHSLVP GETHQQQWCP 70 80 90 100 110 120 LEERLERLEA EVTDLRKQNR ELQARVVQLE SCECWGPGHT CPEGARWEPD ACTACVCRDG 130 140 150 160 170 180 TAHCGPQPNL PHCRGCSHNG QSYGHGETFS PDACTTCRCL AGTVQCQGPS CSELNCLESF 190 200 210 220 230 240 IPPGECCPIC RPGCEYEGQL HQEGSSFLSS SNPCLQCSCL RSLVRCVPVK CQPSPCLNPV 250 260 270 280 290 300 PRLGHCCPVC QASGCTEGNS HRDHGQEWTT PGDPCRICQC LEGHIQCRQR ECASLCPYPA 310 320 330 340 350 360 RPLPGTCCPV CDGCFLNGRE HSSGEPVGSQ DPCSSCRCTN GSVQCEPLPC PPAPCRYPGR 370 380 390 400 410 420 IPGQCCPVCD GCKYQGHEYR SQETFTLQEN GRCLRCVCQA GEVSCEEQDC PVTPCVRSAS 430 440 450 460 470 480 GPQLCSACVL NGEEFAEGIQ WEPDDQPCTS CSCQDGVPVC RAVLCSPVPC QHPTQPPGAC 490 500 510 520 530 540 CPSCDSCTYH SLVYANGQNF TDVDSPCQTC YCEDGTVRCS LINCPFTTCA KPQNGPGQCC 550 560 570 580 590 600 PKCPDCILEA QVFVDGERFP HPRDPCQECW CQEGQAHCQL RACPSAPCVH PLPGTCCKND 610 620 630 640 650 660 CTGCAFGGKE YPNGADFPHP TDPCRLCRCL SGNVQCLARR CPPLSCPQPV LTPGDCCPQC 670 680 690 700 710 720 PDAPADCPQS GNMVPVRHQE HFFQPGDPCS RCLCLDGSVS CQRLTCPPAP CAHPRRDACC 730 740 750 760 770 780 PSCDGCLYQG KEFASGERFP SPNVACHVCL CWEGSVKCEP RTCAPAQCPF PTREDCCPAC 790 800 810 820 830 840 DSCDYLGVSY LSSQEFPDPR EACNLCTCLG GFVTCTRRPC EPPACSHPLI VPEHCCPTCQ 850 860 870 880 890 900 GCLYHGITAA LGETLPDPLD PTCSLCTCEE GSMRCQKKPC PPAPCAHPSP GPCFCPVCRS 910 920 930 940 950 960 CLSQGREHQD GEEFEGPEGS CERCRCLAGQ VSCTRLQCPS LPCLHQVTEP GTCCPRCTGC 970 980 990 1000 1010 1020 LARGEEHPEG SSWVPADSPC SSCMCHKGII TCAQVQCVSA CIWPQEGPSD CCPQCSGCEH 1030 1040 1050 1060 1070 1080 GGRKYEPGES FQPGADPCEV CICKQKREGP PSLHCSRRQC PSLVGCPPSQ LLPPGPQHCC 1090 1100 1110 1120 1130 1140 PTCAQALSNC TEDLVGSELV PPDPCYTCQC QDLTWLCTHR ACPELSCPLW ERHTTPGSCC 1150 1160 1170 1180 1190 1200 PVCKDPTQSC MHQGRWVASG EQWAVDACTS CSCVAGTVHC QTQRCRKLAC SRDEVPALSP 1210 1220 1230 1240 1250 1260 GSCCLRCLPR PASCMAFGDP HYRTFDGRLL HFQGSCSYVL AKDCHGEDFS VHVTNDDRGR 1270 1280 1290 1300 1310 1320 RGVAWTQEVA VLLGTVAVRL LQGRTVMVDQ HTVTLPFLRE PLLYIELRGH TVILHAQPGL 1330 1340 1350 1360 1370 1380 QVLWDGQSQV EVRVPSSYRG QTCGLCGNFN GFAQDDLQGP DGRLLPTEAS FGNSWKVPKG 1390 1400 1410 1420 1430 1440 LGPGRPCSAG REVDPCRAAG YRARREANAR CGILKTSPFS HCHAVVPPEP FFAACVYDLC 1450 1460 1470 1480 1490 1500 ACGPGSSSDT CLCDALEAYA SHCRQAGVTP VWRGPTLCVV GCPVDRGFVF DECGPPCPRT 1510 1520 1530 1540 1550 CFNRHIPLGE LAAHCVRPCV PGCQCPAGLV EHEGHCISPE VCPPVLLTGD
	Isoform 2 [UniParc]. Checksum: 01A83D89AB0109E6	FASTA	1,255	135,115
	10 20 30 40 50 60 MAGARAALLP LLLHLGSLAL AARGGEVSRE QPRLADAISQ QQAPSHSLVP GETHQQQWCP 70 80 90 100 110 120 LEERLERLEA EVTDLRKQNR ELQARVVQLE SCECWGPGHT CPEGARWEPD ACTACVCRDG 130 140 150 160 170 180 TAHCGPQPNL PHCRGCSHNG QSYGHGETFS PDACTTCRCL AGTVQCQGPS CSELNCLESF 190 200 210 220 230 240 IPPGECCPIC RPGCEYEGQL HQEGSSFLSS SNPCLQCSCL RSLVRCVPVK CQPSPCLNPV 250 260 270 280 290 300 PRLGHCCPVC QASGCTEGNS HRDHGQEWTT PGDPCRICQC LEGHIQCRQR ECASLCPYPA 310 320 330 340 350 360 RPLPGTCCPV CDGCFLNGRE HSSGEPVGSQ DPCSSCRCTN GSVQCEPLPC PPAPCRYPGR 370 380 390 400 410 420 IPGQCCPVCD GCKYQGHEYR SQETFTLQEN GRCLRCVCQA GEVSCEEQDC PVTPCVRSAS 430 440 450 460 470 480 GPQLCSACVL NGEEFAEGIQ WEPDDQPCTS CSCQDGVPVC RAVLCSPVPC QHPTQPPGAC 490 500 510 520 530 540 CPSCDSCTYH SLVYANGQNF TDVDSPCQTC YCEDGTVRCS LINCPFTTCA KPQNGPGQCC 550 560 570 580 590 600 PKCPDCILEA QVFVDGERFP HPRDPCQECW CQEGQAHCQL RACPSAPCVH PLPGTCCKND 610 620 630 640 650 660 CTGCAFGGKE YPNGADFPHP TDPCRLCRCL SGNVQCLARR CPPLSCPQPV LTPGDCCPQC 670 680 690 700 710 720 PDAPADCPQS GNMVPVRHQE HFFQPGDPCS RCLCLDGSVS CQRLTCPPAP CAHPRRDACC 730 740 750 760 770 780 PSCDGCLYQG KEFASGERFP SPNVACHVCL CWEGSVKCEP RTCAPAQCPF PTREDCCPAC 790 800 810 820 830 840 DSCDYLGVSY LSSQEFPDPR EACNLCTCLG GFVTCTRRPC EPPACSHPLI VPEHCCPTCQ 850 860 870 880 890 900 GCLYHGITAA LGETLPDPLD PTCSLCTCEE GSMRCQKKPC PPAPCAHPSP GPCFCPVCRS 910 920 930 940 950 960 CLSQGREHQD GEEFEGPEGS CERCRCLAGQ VSCTRLQCPS LPCLHQVTEP GTCCPRCTGC 970 980 990 1000 1010 1020 LARGEEHPEG SSWVPADSPC SSCMCHKGII TCAQVQCVSA CIWPQEGPSD CCPQCSGCEH 1030 1040 1050 1060 1070 1080 GGRKYEPGES FQPGADPCEV CICKQKREGP PSLHCSRRQC PSLVGCPPSQ LLPPGPQHCC 1090 1100 1110 1120 1130 1140 PTCAQALSNC TEDLVGSELV PPDPCYTCQC QDLTWLCTHR ACPELSCPLW ERHTTPGSCC 1150 1160 1170 1180 1190 1200 PVCKDPTQSC MHQGRWVASG EQWAVDACTS CSCVAGTVHC QTQRCRKLAC SRDEVPALSP 1210 1220 1230 1240 1250 GSCCLRCLPR PASCMAFGDP HYRTFDGRLL HFQGSCSYVL AKDCHGEDFR WTSTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kielin/chordin-like protein, a novel enhancer of BMP signaling, attenuates renal fibrotic disease." Lin J., Patel S.R., Cheng X., Cho E.A., Levitan I., Ullenbruch M., Phan S.H., Park J.M., Dressler G.R. Nat. Med. 11:387-393(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INTERACTION WITH BMP7, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INDUCTION BY RENAL STRESSES. Strain: FVB/N.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: NOD.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The cysteine-rich domain protein KCP is a suppressor of transforming growth factor beta/activin signaling in renal epithelia." Lin J., Patel S.R., Wang M., Dressler G.R. Mol. Cell. Biol. 26:4577-4585(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ACTIVIN A AND TGFB1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY884211 mRNA. Translation: AAX77677.1. AK154369 mRNA. Translation: BAE32541.1. AC079276 Genomic DNA. No translation available.
IPI	IPI00271455. IPI00761497.
RefSeq	NP_001025156.3. NM_001029985.4.
UniGene	Mm.332961.
3D structure databases
ProteinModelPortal	Q3U492.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000099135.
Proteomic databases
PRIDE	Q3U492.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000078112; ENSMUSP00000077251; ENSMUSG00000059022. ENSMUST00000101614; ENSMUSP00000099135; ENSMUSG00000059022.
GeneID	333088.
KEGG	mmu:333088.
UCSC	uc009bds.1. mouse.
Organism-specific databases
CTD	375616.
MGI	MGI:2141640. Kcp.
Phylogenomic databases
eggNOG	NOG283828.
GeneTree	ENSGT00690000101825.
HOGENOM	HOG000168484.
InParanoid	Q3U492.
OMA	LVGCPPS.
Gene expression databases
ArrayExpress	Q3U492.
Bgee	Q3U492.
CleanEx	MM_CRIM2.
Genevestigator	Q3U492.
Family and domain databases
InterPro	IPR002919. TIL_dom. IPR014853. Unchr_dom_Cys-rich. IPR001007. VWF_C. IPR001846. VWF_type-D. [Graphical view]
Pfam	PF00093. VWC. 9 hits. PF00094. VWD. 1 hit. [Graphical view]
SMART	SM00832. C8. 1 hit. SM00214. VWC. 17 hits. SM00216. VWD. 1 hit. [Graphical view]
SUPFAM	SSF57567. Cysrich_TIL. 1 hit.
PROSITE	PS01208. VWFC_1. 13 hits. PS50184. VWFC_2. 14 hits. PS51233. VWFD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	399938.
SOURCE	Search...

Entry information

Entry name

KCP_MOUSE

Accession

Primary (citable) accession number: Q3U492
Secondary accession number(s): E9QJU0, Q56NI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program