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Protein

Cytoplasmic tRNA 2-thiolation protein 2

Gene

Ctu2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.UniRule annotation

Pathwayi: 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis

This protein is involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis and in tRNA modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processtRNA processing

Enzyme and pathway databases

UniPathwayiUPA00988

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic tRNA 2-thiolation protein 2UniRule annotation
Gene namesi
Name:Ctu2
Synonyms:Ncs2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1914215 Ctu2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002891762 – 514Cytoplasmic tRNA 2-thiolation protein 2Add BLAST513

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylcysteineBy similarity1
Modified residuei421PhosphoserineBy similarity1
Modified residuei425PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3U308
MaxQBiQ3U308
PaxDbiQ3U308
PeptideAtlasiQ3U308
PRIDEiQ3U308

PTM databases

iPTMnetiQ3U308
PhosphoSitePlusiQ3U308

Expressioni

Gene expression databases

BgeeiENSMUSG00000049482
CleanExiMM_2310061F22RIK
ExpressionAtlasiQ3U308 baseline and differential
GenevisibleiQ3U308 MM

Interactioni

Subunit structurei

Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112113

Structurei

3D structure databases

ProteinModelPortaliQ3U308
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTU2/NCS2 family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2594 Eukaryota
ENOG410YGU3 LUCA
GeneTreeiENSGT00390000008797
HOGENOMiHOG000007287
HOVERGENiHBG107783
InParanoidiQ3U308
KOiK14169
OMAiMCSIGED
OrthoDBiEOG091G0JFR
PhylomeDBiQ3U308
TreeFamiTF313203

Family and domain databases

HAMAPiMF_03054 CTU2, 1 hit
InterProiView protein in InterPro
IPR019407 CTU2
PANTHERiPTHR20882 PTHR20882, 1 hit
PfamiView protein in Pfam
PF10288 CTU2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3U308-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCQAGEDYAG PARREPPPVP RPSREQKCVK CAEGLPVVVI RAGDAFCRVC
60 70 80 90 100
FKAFYVHKFR AMLGKNRVIF PGEKVLLSWS GGPSSSSMVW QVLEGLSQDS
110 120 130 140 150
AKRLRFVPGV IYVDEGAACG QSLEDRQKTV AEVKRILENT GFPWHVVALE
160 170 180 190 200
EVFSLPPSVL CCTSQESAGT EEAYKAAVDR FLQQQQQQQQ RVLGAEAGAS
210 220 230 240 250
PAQGEARLHP SHGREPSGTA GYPTAAQTEA LSRLFSSIKT LTAKEELLQT
260 270 280 290 300
LRTHLIVHIA RVHGYCKVMT GETCTRLAIK LMTNLALGRG AFLAWDTGFS
310 320 330 340 350
DERHGDVVLV RPMRDHTLKE VAFYNHLFRV PSVFTPAIDT KAPEKASIHR
360 370 380 390 400
LMEAFILRLQ TLFPSTVSTV YRTSEKLVKA PREGCAAGPS GPSCLLCMCA
410 420 430 440 450
LDIDTADSAT AFGAQSSSHL SQMPSAEAGM PTQPCCAAGE GQAQSCHREV
460 470 480 490 500
GKRGDARACI TEQLCYSCRV NMKDLPSLDP LPPYVLAEAQ LRSQRGSVSE
510
EIQEYLITDE EEDS
Length:514
Mass (Da):56,105
Last modified:October 11, 2005 - v1
Checksum:i88A531D4F3BD3532
GO

Sequence cautioni

The sequence AAH36332 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC41181 differs from that shown. Reason: Frameshift at position 250.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK090349 mRNA Translation: BAC41181.1 Frameshift.
AK154995 mRNA Translation: BAE32982.1
BC036332 mRNA Translation: AAH36332.1 Different initiation.
CCDSiCCDS52694.1
RefSeqiNP_722470.2, NM_153775.2
UniGeneiMm.482288

Genome annotation databases

EnsembliENSMUST00000116412; ENSMUSP00000112113; ENSMUSG00000049482
GeneIDi66965
KEGGimmu:66965
UCSCiuc009nsz.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCTU2_MOUSE
AccessioniPrimary (citable) accession number: Q3U308
Secondary accession number(s): Q8BTG9, Q8CI68
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2005
Last modified: May 23, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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