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Q3U308

- CTU2_MOUSE

UniProt

Q3U308 - CTU2_MOUSE

Protein

Cytoplasmic tRNA 2-thiolation protein 2

Gene

Ctu2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. nucleotidyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB

    GO - Biological processi

    1. protein urmylation Source: UniProtKB-HAMAP
    2. tRNA thio-modification Source: UniProtKB
    3. tRNA wobble uridine modification Source: UniProtKB

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    UniPathwayiUPA00988.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytoplasmic tRNA 2-thiolation protein 2UniRule annotation
    Gene namesi
    Name:Ctu2
    Synonyms:Ncs2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1914215. Ctu2.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. mitochondrion Source: Ensembl
    3. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 514513Cytoplasmic tRNA 2-thiolation protein 2PRO_0000289176Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylcysteineBy similarity
    Modified residuei421 – 4211PhosphoserineBy similarity
    Modified residuei425 – 4251PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ3U308.
    PRIDEiQ3U308.

    PTM databases

    PhosphoSiteiQ3U308.

    Expressioni

    Gene expression databases

    BgeeiQ3U308.
    CleanExiMM_2310061F22RIK.
    GenevestigatoriQ3U308.

    Interactioni

    Subunit structurei

    Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ3U308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CTU2/NCS2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiNOG308629.
    GeneTreeiENSGT00390000008797.
    HOGENOMiHOG000007287.
    HOVERGENiHBG107783.
    InParanoidiQ3U308.
    KOiK14169.
    OMAiCRDCFKA.
    OrthoDBiEOG7C2R1T.
    PhylomeDBiQ3U308.
    TreeFamiTF313203.

    Family and domain databases

    HAMAPiMF_03054. CTU2.
    InterProiIPR019407. Ncs2/Tuc2/Ctu2.
    [Graphical view]
    PfamiPF10288. DUF2392. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q3U308-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCQAGEDYAG PARREPPPVP RPSREQKCVK CAEGLPVVVI RAGDAFCRVC    50
    FKAFYVHKFR AMLGKNRVIF PGEKVLLSWS GGPSSSSMVW QVLEGLSQDS 100
    AKRLRFVPGV IYVDEGAACG QSLEDRQKTV AEVKRILENT GFPWHVVALE 150
    EVFSLPPSVL CCTSQESAGT EEAYKAAVDR FLQQQQQQQQ RVLGAEAGAS 200
    PAQGEARLHP SHGREPSGTA GYPTAAQTEA LSRLFSSIKT LTAKEELLQT 250
    LRTHLIVHIA RVHGYCKVMT GETCTRLAIK LMTNLALGRG AFLAWDTGFS 300
    DERHGDVVLV RPMRDHTLKE VAFYNHLFRV PSVFTPAIDT KAPEKASIHR 350
    LMEAFILRLQ TLFPSTVSTV YRTSEKLVKA PREGCAAGPS GPSCLLCMCA 400
    LDIDTADSAT AFGAQSSSHL SQMPSAEAGM PTQPCCAAGE GQAQSCHREV 450
    GKRGDARACI TEQLCYSCRV NMKDLPSLDP LPPYVLAEAQ LRSQRGSVSE 500
    EIQEYLITDE EEDS 514
    Length:514
    Mass (Da):56,105
    Last modified:October 11, 2005 - v1
    Checksum:i88A531D4F3BD3532
    GO

    Sequence cautioni

    The sequence BAC41181.1 differs from that shown. Reason: Frameshift at position 250.
    The sequence AAH36332.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK090349 mRNA. Translation: BAC41181.1. Frameshift.
    AK154995 mRNA. Translation: BAE32982.1.
    BC036332 mRNA. Translation: AAH36332.1. Different initiation.
    CCDSiCCDS52694.1.
    RefSeqiNP_722470.2. NM_153775.2.
    UniGeneiMm.482288.

    Genome annotation databases

    EnsembliENSMUST00000116412; ENSMUSP00000112113; ENSMUSG00000049482.
    GeneIDi66965.
    KEGGimmu:66965.
    UCSCiuc009nsz.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK090349 mRNA. Translation: BAC41181.1 . Frameshift.
    AK154995 mRNA. Translation: BAE32982.1 .
    BC036332 mRNA. Translation: AAH36332.1 . Different initiation.
    CCDSi CCDS52694.1.
    RefSeqi NP_722470.2. NM_153775.2.
    UniGenei Mm.482288.

    3D structure databases

    ProteinModelPortali Q3U308.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q3U308.

    Proteomic databases

    PaxDbi Q3U308.
    PRIDEi Q3U308.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000116412 ; ENSMUSP00000112113 ; ENSMUSG00000049482 .
    GeneIDi 66965.
    KEGGi mmu:66965.
    UCSCi uc009nsz.2. mouse.

    Organism-specific databases

    CTDi 348180.
    MGIi MGI:1914215. Ctu2.

    Phylogenomic databases

    eggNOGi NOG308629.
    GeneTreei ENSGT00390000008797.
    HOGENOMi HOG000007287.
    HOVERGENi HBG107783.
    InParanoidi Q3U308.
    KOi K14169.
    OMAi CRDCFKA.
    OrthoDBi EOG7C2R1T.
    PhylomeDBi Q3U308.
    TreeFami TF313203.

    Enzyme and pathway databases

    UniPathwayi UPA00988 .

    Miscellaneous databases

    NextBioi 323147.
    PROi Q3U308.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3U308.
    CleanExi MM_2310061F22RIK.
    Genevestigatori Q3U308.

    Family and domain databases

    HAMAPi MF_03054. CTU2.
    InterProi IPR019407. Ncs2/Tuc2/Ctu2.
    [Graphical view ]
    Pfami PF10288. DUF2392. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Diencephalon.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiCTU2_MOUSE
    AccessioniPrimary (citable) accession number: Q3U308
    Secondary accession number(s): Q8BTG9, Q8CI68
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3