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Q3U308 (CTU2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytoplasmic tRNA 2-thiolation protein 2
Gene names
Name:Ctu2
Synonyms:Ncs2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position By similarity. HAMAP-Rule MF_03054

Pathway

tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis. HAMAP-Rule MF_03054

Subunit structure

Component of a complex at least composed of URM1, CTU2/NCS2 and CTU1/ATPBD3 By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_03054.

Sequence similarities

Belongs to the CTU2/NCS2 family.

Sequence caution

The sequence AAH36332.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC41181.1 differs from that shown. Reason: Frameshift at position 250.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 514513Cytoplasmic tRNA 2-thiolation protein 2 HAMAP-Rule MF_03054
PRO_0000289176

Amino acid modifications

Modified residue21N-acetylcysteine By similarity
Modified residue4211Phosphoserine By similarity
Modified residue4251Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3U308 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 88A531D4F3BD3532

FASTA51456,105
        10         20         30         40         50         60 
MCQAGEDYAG PARREPPPVP RPSREQKCVK CAEGLPVVVI RAGDAFCRVC FKAFYVHKFR 

        70         80         90        100        110        120 
AMLGKNRVIF PGEKVLLSWS GGPSSSSMVW QVLEGLSQDS AKRLRFVPGV IYVDEGAACG 

       130        140        150        160        170        180 
QSLEDRQKTV AEVKRILENT GFPWHVVALE EVFSLPPSVL CCTSQESAGT EEAYKAAVDR 

       190        200        210        220        230        240 
FLQQQQQQQQ RVLGAEAGAS PAQGEARLHP SHGREPSGTA GYPTAAQTEA LSRLFSSIKT 

       250        260        270        280        290        300 
LTAKEELLQT LRTHLIVHIA RVHGYCKVMT GETCTRLAIK LMTNLALGRG AFLAWDTGFS 

       310        320        330        340        350        360 
DERHGDVVLV RPMRDHTLKE VAFYNHLFRV PSVFTPAIDT KAPEKASIHR LMEAFILRLQ 

       370        380        390        400        410        420 
TLFPSTVSTV YRTSEKLVKA PREGCAAGPS GPSCLLCMCA LDIDTADSAT AFGAQSSSHL 

       430        440        450        460        470        480 
SQMPSAEAGM PTQPCCAAGE GQAQSCHREV GKRGDARACI TEQLCYSCRV NMKDLPSLDP 

       490        500        510 
LPPYVLAEAQ LRSQRGSVSE EIQEYLITDE EEDS 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Diencephalon.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK090349 mRNA. Translation: BAC41181.1. Frameshift.
AK154995 mRNA. Translation: BAE32982.1.
BC036332 mRNA. Translation: AAH36332.1. Different initiation.
CCDSCCDS52694.1.
RefSeqNP_722470.2. NM_153775.2.
UniGeneMm.482288.

3D structure databases

ProteinModelPortalQ3U308.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ3U308.

Proteomic databases

PaxDbQ3U308.
PRIDEQ3U308.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000116412; ENSMUSP00000112113; ENSMUSG00000049482.
GeneID66965.
KEGGmmu:66965.
UCSCuc009nsz.2. mouse.

Organism-specific databases

CTD348180.
MGIMGI:1914215. Ctu2.

Phylogenomic databases

eggNOGNOG308629.
GeneTreeENSGT00390000008797.
HOGENOMHOG000007287.
HOVERGENHBG107783.
InParanoidQ3U308.
KOK14169.
OMACRDCFKA.
OrthoDBEOG7C2R1T.
PhylomeDBQ3U308.
TreeFamTF313203.

Enzyme and pathway databases

UniPathwayUPA00988.

Gene expression databases

BgeeQ3U308.
CleanExMM_2310061F22RIK.
GenevestigatorQ3U308.

Family and domain databases

HAMAPMF_03054. CTU2.
InterProIPR019407. Ncs2/Tuc2/Ctu2.
[Graphical view]
PfamPF10288. DUF2392. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio323147.
PROQ3U308.
SOURCESearch...

Entry information

Entry nameCTU2_MOUSE
AccessionPrimary (citable) accession number: Q3U308
Secondary accession number(s): Q8BTG9, Q8CI68
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot