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Protein

Voltage-gated hydrogen channel 1

Gene

Hvcn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.5 Publications

Enzyme regulationi

The dimers display cooperative channel gating. The channel activity is inhibited by zinc ions.4 Publications

GO - Molecular functioni

  • voltage-gated proton channel activity Source: HGNC

GO - Biological processi

  • cellular response to pH Source: UniProtKB
  • cellular response to zinc ion Source: UniProtKB
  • proton transport Source: HGNC
  • response to pH Source: HGNC
  • response to zinc ion Source: HGNC
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1300642. Sperm Motility And Taxes.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

TCDBi1.A.51.1.1. the voltage-gated proton channel (vpc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-gated hydrogen channel 1
Alternative name(s):
Hydrogen voltage-gated channel 1
Short name:
HV1
Voltage sensor domain-only protein
Short name:
mVSOP
Gene namesi
Name:Hvcn1
Synonyms:Bts, Vsop
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1921346. Hvcn1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 96CytoplasmicSequence analysisAdd BLAST96
Transmembranei97 – 117Helical; Name=Segment S1Add BLAST21
Topological domaini118 – 134ExtracellularSequence analysisAdd BLAST17
Transmembranei135 – 157Helical; Name=Segment S2Add BLAST23
Topological domaini158 – 165CytoplasmicSequence analysis8
Transmembranei166 – 186Helical; Name=Segment S3Add BLAST21
Topological domaini187 – 193ExtracellularSequence analysis7
Transmembranei194 – 214Helical; Name=Segment S4Add BLAST21
Topological domaini215 – 269CytoplasmicSequence analysisAdd BLAST55

GO - Cellular componenti

  • integral component of membrane Source: HGNC
  • integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi201R → Q: Faster channel activation kinetics. 1 Publication1
Mutagenesisi207R → Q: Same activation kinetics as wild-type. 1 Publication1
Mutagenesisi216 – 218VKT → GGG: Impairs gating cooperativity. 1 Publication3
Mutagenesisi230 – 232INI → NIN: Slows channel activation. 1 Publication3
Mutagenesisi231N → L: No effect on channel activation kinetics. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003421881 – 269Voltage-gated hydrogen channel 1Add BLAST269

Post-translational modificationi

Phosphorylation may enhance channel gating.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3U2S8.
MaxQBiQ3U2S8.
PaxDbiQ3U2S8.
PRIDEiQ3U2S8.
TopDownProteomicsiQ3U2S8.

PTM databases

iPTMnetiQ3U2S8.
PhosphoSitePlusiQ3U2S8.

Expressioni

Tissue specificityi

Enriched in immune tissues, such as bone marrow, macrophages and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000064267.
ExpressionAtlasiQ3U2S8. baseline and differential.
GenevisibleiQ3U2S8. MM.

Interactioni

Subunit structurei

Homodimer.3 Publications

Protein-protein interaction databases

DIPiDIP-46134N.
IntActiQ3U2S8. 1 interactor.
MINTiMINT-4128643.
STRINGi10090.ENSMUSP00000072401.

Structurei

Secondary structure

1269
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi87 – 90Combined sources4
Helixi96 – 120Combined sources25
Helixi134 – 155Combined sources22
Helixi164 – 184Combined sources21
Helixi193 – 215Combined sources23
Helixi220 – 261Combined sources42

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VMXX-ray1.45A/B/C/D220-266[»]
3VMYX-ray1.47A/B/C/D220-269[»]
3VMZX-ray1.55A/B/C/D220-269[»]
3VN0X-ray1.37A/B/C/D220-269[»]
3VYIX-ray2.30A/B/C/D/E/F/G/H/I/J/K/L220-269[»]
3WKVX-ray3.45A73-215[»]
ProteinModelPortaliQ3U2S8.
SMRiQ3U2S8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili221 – 2612 PublicationsAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 53Poly-Glu8
Compositional biasi173 – 176Poly-Val4

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Unlike other voltage-gated ion channels it lacks the pore domain.1 Publication
The C-terminal coiled coil region mediates homodimerization and cooperative channel gating. It is essential for normal subcellular localization.1 Publication

Sequence similaritiesi

Belongs to the hydrogen channel family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKBG. Eukaryota.
ENOG4111XHU. LUCA.
GeneTreeiENSGT00530000063670.
HOGENOMiHOG000067871.
HOVERGENiHBG102207.
InParanoidiQ3U2S8.
OMAiRMSKFLK.
OrthoDBiEOG091G0L8G.
PhylomeDBiQ3U2S8.
TreeFamiTF332056.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR031844. VGPC1_C.
[Graphical view]
PfamiPF00520. Ion_trans. 1 hit.
PF16799. VGPC1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3U2S8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSHDPKAVT RRTKVAPTKR MSRFLKHFTV VGDDYHTWNV NYKKWENEEE
60 70 80 90 100
EEEPAPTSAE GEGNAEGPDA EAGSASTPRQ SLDFRSRLRK LFSSHRFQVI
110 120 130 140 150
IICLVVLDAL LVLAELLLDL KIIEPDEQDY AVTAFHYMSF AILVFFMLEI
160 170 180 190 200
FFKIFVFRLE FFHHKFEILD AFVVVVSFVL DLVLLFKSHH FEALGLLILL
210 220 230 240 250
RLWRVARIIN GIIISVKTRS ERQILRLKQI NIQLATKIQH LEFSCSEKEQ
260
EIERLNKLLK QNGLLGDVN
Length:269
Mass (Da):31,242
Last modified:July 1, 2008 - v2
Checksum:iB549CB553DEB6568
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66E → A in BAE33062 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002854 mRNA. Translation: BAB22409.1.
AK154880 mRNA. Translation: BAE32899.1.
AK155123 mRNA. Translation: BAE33062.1.
AK170422 mRNA. Translation: BAE41787.1.
BC021548 mRNA. Translation: AAH21548.1.
CCDSiCCDS19644.1.
RefSeqiNP_001035954.1. NM_001042489.2.
NP_083028.1. NM_028752.3.
XP_006530535.1. XM_006530472.2.
XP_006530536.1. XM_006530473.1.
UniGeneiMm.28804.

Genome annotation databases

EnsembliENSMUST00000072602; ENSMUSP00000072401; ENSMUSG00000064267.
ENSMUST00000100747; ENSMUSP00000098312; ENSMUSG00000064267.
GeneIDi74096.
KEGGimmu:74096.
UCSCiuc008zku.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002854 mRNA. Translation: BAB22409.1.
AK154880 mRNA. Translation: BAE32899.1.
AK155123 mRNA. Translation: BAE33062.1.
AK170422 mRNA. Translation: BAE41787.1.
BC021548 mRNA. Translation: AAH21548.1.
CCDSiCCDS19644.1.
RefSeqiNP_001035954.1. NM_001042489.2.
NP_083028.1. NM_028752.3.
XP_006530535.1. XM_006530472.2.
XP_006530536.1. XM_006530473.1.
UniGeneiMm.28804.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VMXX-ray1.45A/B/C/D220-266[»]
3VMYX-ray1.47A/B/C/D220-269[»]
3VMZX-ray1.55A/B/C/D220-269[»]
3VN0X-ray1.37A/B/C/D220-269[»]
3VYIX-ray2.30A/B/C/D/E/F/G/H/I/J/K/L220-269[»]
3WKVX-ray3.45A73-215[»]
ProteinModelPortaliQ3U2S8.
SMRiQ3U2S8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46134N.
IntActiQ3U2S8. 1 interactor.
MINTiMINT-4128643.
STRINGi10090.ENSMUSP00000072401.

Protein family/group databases

TCDBi1.A.51.1.1. the voltage-gated proton channel (vpc) family.

PTM databases

iPTMnetiQ3U2S8.
PhosphoSitePlusiQ3U2S8.

Proteomic databases

EPDiQ3U2S8.
MaxQBiQ3U2S8.
PaxDbiQ3U2S8.
PRIDEiQ3U2S8.
TopDownProteomicsiQ3U2S8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072602; ENSMUSP00000072401; ENSMUSG00000064267.
ENSMUST00000100747; ENSMUSP00000098312; ENSMUSG00000064267.
GeneIDi74096.
KEGGimmu:74096.
UCSCiuc008zku.2. mouse.

Organism-specific databases

CTDi84329.
MGIiMGI:1921346. Hvcn1.

Phylogenomic databases

eggNOGiENOG410IKBG. Eukaryota.
ENOG4111XHU. LUCA.
GeneTreeiENSGT00530000063670.
HOGENOMiHOG000067871.
HOVERGENiHBG102207.
InParanoidiQ3U2S8.
OMAiRMSKFLK.
OrthoDBiEOG091G0L8G.
PhylomeDBiQ3U2S8.
TreeFamiTF332056.

Enzyme and pathway databases

ReactomeiR-MMU-1300642. Sperm Motility And Taxes.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiHvcn1. mouse.
PROiQ3U2S8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064267.
ExpressionAtlasiQ3U2S8. baseline and differential.
GenevisibleiQ3U2S8. MM.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR031844. VGPC1_C.
[Graphical view]
PfamiPF00520. Ion_trans. 1 hit.
PF16799. VGPC1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHVCN1_MOUSE
AccessioniPrimary (citable) accession number: Q3U2S8
Secondary accession number(s): Q9DCE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: November 30, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.