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Q3U2S8

- HVCN1_MOUSE

UniProt

Q3U2S8 - HVCN1_MOUSE

Protein

Voltage-gated hydrogen channel 1

Gene

Hvcn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis.5 Publications

    Enzyme regulationi

    The dimers display cooperative channel gating. The channel activity is inhibited by zinc ions.4 Publications

    GO - Molecular functioni

    1. voltage-gated proton channel activity Source: HGNC

    GO - Biological processi

    1. cellular response to pH Source: UniProtKB
    2. cellular response to zinc ion Source: UniProtKB
    3. proton transport Source: HGNC
    4. response to pH Source: HGNC
    5. response to zinc ion Source: HGNC

    Keywords - Molecular functioni

    Ion channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196630. Sperm Motility And Taxes.

    Protein family/group databases

    TCDBi1.A.51.1.1. the voltage-gated proton channel (vpc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Voltage-gated hydrogen channel 1
    Alternative name(s):
    Hydrogen voltage-gated channel 1
    Short name:
    HV1
    Voltage sensor domain-only protein
    Short name:
    mVSOP
    Gene namesi
    Name:Hvcn1
    Synonyms:Bts, Vsop
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1921346. Hvcn1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: HGNC
    2. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi201 – 2011R → Q: Faster channel activation kinetics. 1 Publication
    Mutagenesisi207 – 2071R → Q: Same activation kinetics as wild-type. 1 Publication
    Mutagenesisi216 – 2183VKT → GGG: Impairs gating cooperativity.
    Mutagenesisi230 – 2323INI → NIN: Slows channel activation.
    Mutagenesisi231 – 2311N → L: No effect on channel activation kinetics. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Voltage-gated hydrogen channel 1PRO_0000342188Add
    BLAST

    Post-translational modificationi

    Phosphorylation may enhance channel gating.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ3U2S8.
    PaxDbiQ3U2S8.
    PRIDEiQ3U2S8.

    PTM databases

    PhosphoSiteiQ3U2S8.

    Expressioni

    Tissue specificityi

    Enriched in immune tissues, such as bone marrow, macrophages and spleen.1 Publication

    Gene expression databases

    ArrayExpressiQ3U2S8.
    BgeeiQ3U2S8.
    GenevestigatoriQ3U2S8.

    Interactioni

    Subunit structurei

    Homodimer.3 Publications

    Protein-protein interaction databases

    DIPiDIP-46134N.
    IntActiQ3U2S8. 1 interaction.
    MINTiMINT-4128643.
    STRINGi10090.ENSMUSP00000072401.

    Structurei

    Secondary structure

    1
    269
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi87 – 904
    Helixi96 – 12025
    Helixi134 – 15522
    Helixi164 – 18421
    Helixi193 – 21523
    Helixi220 – 26142

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VMXX-ray1.45A/B/C/D220-266[»]
    3VMYX-ray1.47A/B/C/D220-269[»]
    3VMZX-ray1.55A/B/C/D220-269[»]
    3VN0X-ray1.37A/B/C/D220-269[»]
    3VYIX-ray2.30A/B/C/D/E/F/G/H/I/J/K/L220-269[»]
    3WKVX-ray3.45A73-215[»]
    ProteinModelPortaliQ3U2S8.
    SMRiQ3U2S8. Positions 87-208, 220-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9696CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini118 – 13417ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini158 – 1658CytoplasmicSequence Analysis
    Topological domaini187 – 1937ExtracellularSequence Analysis
    Topological domaini215 – 26955CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei97 – 11721Helical; Name=Segment S1Add
    BLAST
    Transmembranei135 – 15723Helical; Name=Segment S2Add
    BLAST
    Transmembranei166 – 18621Helical; Name=Segment S3Add
    BLAST
    Transmembranei194 – 21421Helical; Name=Segment S4Add
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili221 – 261412 PublicationsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi46 – 538Poly-Glu
    Compositional biasi173 – 1764Poly-Val

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Unlike other voltage-gated ion channels it lacks the pore domain.1 Publication
    The C-terminal coiled coil region mediates homodimerization and cooperative channel gating. It is essential for normal subcellular localization.1 Publication

    Sequence similaritiesi

    Belongs to the hydrogen channel family.Curated

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG46709.
    GeneTreeiENSGT00530000063670.
    HOGENOMiHOG000067871.
    HOVERGENiHBG102207.
    InParanoidiQ3U2S8.
    OMAiEFSCSEK.
    PhylomeDBiQ3U2S8.
    TreeFamiTF332056.

    Family and domain databases

    Gene3Di1.20.120.350. 1 hit.
    InterProiIPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    [Graphical view]
    PfamiPF00520. Ion_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3U2S8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSHDPKAVT RRTKVAPTKR MSRFLKHFTV VGDDYHTWNV NYKKWENEEE    50
    EEEPAPTSAE GEGNAEGPDA EAGSASTPRQ SLDFRSRLRK LFSSHRFQVI 100
    IICLVVLDAL LVLAELLLDL KIIEPDEQDY AVTAFHYMSF AILVFFMLEI 150
    FFKIFVFRLE FFHHKFEILD AFVVVVSFVL DLVLLFKSHH FEALGLLILL 200
    RLWRVARIIN GIIISVKTRS ERQILRLKQI NIQLATKIQH LEFSCSEKEQ 250
    EIERLNKLLK QNGLLGDVN 269
    Length:269
    Mass (Da):31,242
    Last modified:July 1, 2008 - v2
    Checksum:iB549CB553DEB6568
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661E → A in BAE33062. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK002854 mRNA. Translation: BAB22409.1.
    AK154880 mRNA. Translation: BAE32899.1.
    AK155123 mRNA. Translation: BAE33062.1.
    AK170422 mRNA. Translation: BAE41787.1.
    BC021548 mRNA. Translation: AAH21548.1.
    CCDSiCCDS19644.1.
    RefSeqiNP_001035954.1. NM_001042489.2.
    NP_083028.1. NM_028752.3.
    XP_006530535.1. XM_006530472.1.
    XP_006530536.1. XM_006530473.1.
    UniGeneiMm.28804.

    Genome annotation databases

    EnsembliENSMUST00000072602; ENSMUSP00000072401; ENSMUSG00000064267.
    ENSMUST00000100747; ENSMUSP00000098312; ENSMUSG00000064267.
    GeneIDi74096.
    KEGGimmu:74096.
    UCSCiuc008zku.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK002854 mRNA. Translation: BAB22409.1 .
    AK154880 mRNA. Translation: BAE32899.1 .
    AK155123 mRNA. Translation: BAE33062.1 .
    AK170422 mRNA. Translation: BAE41787.1 .
    BC021548 mRNA. Translation: AAH21548.1 .
    CCDSi CCDS19644.1.
    RefSeqi NP_001035954.1. NM_001042489.2.
    NP_083028.1. NM_028752.3.
    XP_006530535.1. XM_006530472.1.
    XP_006530536.1. XM_006530473.1.
    UniGenei Mm.28804.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VMX X-ray 1.45 A/B/C/D 220-266 [» ]
    3VMY X-ray 1.47 A/B/C/D 220-269 [» ]
    3VMZ X-ray 1.55 A/B/C/D 220-269 [» ]
    3VN0 X-ray 1.37 A/B/C/D 220-269 [» ]
    3VYI X-ray 2.30 A/B/C/D/E/F/G/H/I/J/K/L 220-269 [» ]
    3WKV X-ray 3.45 A 73-215 [» ]
    ProteinModelPortali Q3U2S8.
    SMRi Q3U2S8. Positions 87-208, 220-266.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-46134N.
    IntActi Q3U2S8. 1 interaction.
    MINTi MINT-4128643.
    STRINGi 10090.ENSMUSP00000072401.

    Protein family/group databases

    TCDBi 1.A.51.1.1. the voltage-gated proton channel (vpc) family.

    PTM databases

    PhosphoSitei Q3U2S8.

    Proteomic databases

    MaxQBi Q3U2S8.
    PaxDbi Q3U2S8.
    PRIDEi Q3U2S8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000072602 ; ENSMUSP00000072401 ; ENSMUSG00000064267 .
    ENSMUST00000100747 ; ENSMUSP00000098312 ; ENSMUSG00000064267 .
    GeneIDi 74096.
    KEGGi mmu:74096.
    UCSCi uc008zku.1. mouse.

    Organism-specific databases

    CTDi 84329.
    MGIi MGI:1921346. Hvcn1.

    Phylogenomic databases

    eggNOGi NOG46709.
    GeneTreei ENSGT00530000063670.
    HOGENOMi HOG000067871.
    HOVERGENi HBG102207.
    InParanoidi Q3U2S8.
    OMAi EFSCSEK.
    PhylomeDBi Q3U2S8.
    TreeFami TF332056.

    Enzyme and pathway databases

    Reactomei REACT_196630. Sperm Motility And Taxes.

    Miscellaneous databases

    ChiTaRSi HVCN1. mouse.
    NextBioi 339755.
    PROi Q3U2S8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q3U2S8.
    Bgeei Q3U2S8.
    Genevestigatori Q3U2S8.

    Family and domain databases

    Gene3Di 1.20.120.350. 1 hit.
    InterProi IPR027359. Channel_four-helix_dom.
    IPR005821. Ion_trans_dom.
    [Graphical view ]
    Pfami PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    3. "A voltage sensor-domain protein is a voltage-gated proton channel."
      Sasaki M., Takagi M., Okamura Y.
      Science 312:589-592(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DOMAIN, ENZYME REGULATION, MUTAGENESIS OF ARG-201 AND ARG-207.
    4. "Voltage-gated proton channels maintain pH in human neutrophils during phagocytosis."
      Morgan D., Capasso M., Musset B., Cherny V.V., Rios E., Dyer M.J., DeCoursey T.E.
      Proc. Natl. Acad. Sci. U.S.A. 106:18022-18027(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION.
    5. "The cytoplasmic coiled-coil mediates cooperative gating temperature sensitivity in the voltage-gated H(+) channel Hv1."
      Fujiwara Y., Kurokawa T., Takeshita K., Kobayashi M., Okochi Y., Nakagawa A., Okamura Y.
      Nat. Commun. 3:816-816(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 220-266, SUBUNIT, FUNCTION, SUBCELLULAR LOCATION, COILED COIL, MUTAGENESIS OF ASN-231; 216-VAL--THR-218 AND 230-ILE--ILE-232, ENZYME REGULATION.
    6. "Gating of the designed trimeric/tetrameric voltage-gated H+ channel."
      Fujiwara Y., Kurokawa T., Takeshita K., Nakagawa A., Larsson H.P., Okamura Y.
      J. Physiol. (Lond.) 591:627-640(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 220-269, SUBUNIT, SUBCELLULAR LOCATION, FUNCTION, COILED COIL.
    7. Cited for: X-RAY CRYSTALLOGRAPHY (3.45 ANGSTROMS) OF 73-215, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, SUBUNIT, ENZYME REGULATION.

    Entry informationi

    Entry nameiHVCN1_MOUSE
    AccessioniPrimary (citable) accession number: Q3U2S8
    Secondary accession number(s): Q9DCE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3