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Reviewed, UniProtKB/Swiss-Prot Q3U2S4 (OTUD5_MOUSE)

Last modified January 19, 2010. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    OTU domain-containing protein 5
    EC=3.1.2.15
Alternative name(s):
    Deubiquinating enzyme A
      Short name=DUBA
Gene names
Name: Otud5
Synonyms: DXImx46e, Sfc7
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production By similarity.

Catalytic activity

Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

Enzyme regulation

Inhibited by N-ethyl-maleimide (NEM) By similarity.

Subunit structure

Interacts with TRAF3 By similarity.

Sequence similarities

Belongs to the peptidase C85 family.

Contains 1 OTU domain.

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Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protease
Thiol protease
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological processmodification-dependent protein catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin thiolesterase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3U2S4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3U2S4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     118-160: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566OTU domain-containing protein 5
PRO_0000278224

Regions

Domain213 – 336124OTU
Compositional bias5 – 113109Pro-rich
Compositional bias33 – 174142Gly-rich

Sites

Active site2211 Potential
Active site2241 Potential
Active site3291 Potential

Amino acid modifications

Modified residue641Phosphoserine By similarity
Modified residue1651Phosphoserine By similarity
Modified residue1751Phosphotyrosine By similarity
Modified residue1771Phosphoserine By similarity
Modified residue4471Phosphoserine By similarity
Modified residue5021Phosphothreonine By similarity
Modified residue5031Phosphoserine Ref.5

Natural variations

Alternative sequence118 – 16043Missing in isoform 2.
VSP_023196

Experimental info

Sequence conflict1991Missing in AAF66952. Ref.3
Sequence conflict3061I → F in AAF66952. Ref.3
Sequence conflict3081T → I in BAE33066. Ref.1
Sequence conflict4361A → V in AAF66952. Ref.3
Sequence conflict5261F → FA in BAE33066. Ref.1
Sequence conflict5321D → Y in BAE29078. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: 89AAD0FF58792EFB

FASTA56660,306
        10         20         30         40         50         60 
MTILPKKKPP PPDADPANEP PPPGPLPPAP RRGAGVGVGG GGTGVGGGER DRDSGVVGAR 

        70         80         90        100        110        120 
PRASPPPQGP LPGPPGALHR WALAVPPGAV AGPRPQQASP PPCGGPGGPG GGPGDALGAT 

       130        140        150        160        170        180 
TAGVGAAGVV VGVGGTVGVG GCCSGPGHSK RRRQAPGVGA VGGASPEREE VGAGYNSEDE 

       190        200        210        220        230        240 
YEAAAARIEA MDPATVEQQE HWFEKALRDK KGFIIKQMKE DGACLFRAVA DQVYGDQDMH 

       250        260        270        280        290        300 
EVVRKHCMDY LMKNADYFSN YVTEDFTTYI NRKRKNNCHG NHIEMQAMAE MYNRPVEVYQ 

       310        320        330        340        350        360 
YSTEPINTFH GIHQNEDEPI RVSYHRNIHY NSVVNPNKAT IGVGLGLPSF KPGFAEQSLM 

       370        380        390        400        410        420 
KNAIKTSEES WIEQQMLEDK KRATDWEATN EAIEEQVARE SYLQWLRDQE KQARQVRGPS 

       430        440        450        460        470        480 
QPRKASATCS SATAAASSGL EEWTSRSPRQ RSSASSPEHP ELHAELGIKP PSPGTVLALA 

       490        500        510        520        530        540 
KPPSPCAPGT SSQFSAGGDR ATSPLVSLYP ALECRALIQQ MSPSAFGLND WDDDEILASV 

       550        560 
LAVSQQEYLD SMKKNKVHRE PPPDKS

« Hide

Isoform 2.

Checksum: 5055D6F15219CAFC
Show »

FASTA52356,548

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References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Bone marrow, Spleen and Tongue.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary gland.
[4]"A transcript map of a 2-Mb BAC contig in the proximal portion of the mouse X chromosome and regional mapping of the scurfy mutation."
Means G.D., Toy D.Y., Baum P.R., Derry J.M.J.
Genomics 65:213-223(2000) [PubMed: 10857745] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-566 (ISOFORMS 1/2).
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-503, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AK009620 mRNA. Translation: BAB26396.1.
AK149777 mRNA. Translation: BAE29078.1.
AK155131 mRNA. Translation: BAE33066.1.
AK171874 mRNA. Translation: BAE42712.1.
BC016529 mRNA. Translation: AAH16529.1.
BC051111 mRNA. Translation: AAH51111.1.
AF229642 mRNA. Translation: AAF66952.1.
AL671995 Genomic DNA. Translation: CAM13606.1.
AL671978 Genomic DNA. Translation: CAM24561.1.
IPIIPI00775941.
IPI00776134.
RefSeqNP_613070.2.
UniGeneMm.142827

3D structure databases

SMRQ3U2S4. Positions 208-335.
ModBaseSearch...

PTM databases

PhosphoSiteQ3U2S4.

Proteomic databases

PRIDEQ3U2S4.

Genome annotation databases

EnsemblENSMUST00000033494; ENSMUSP00000033494; ENSMUSG00000031154; Mus musculus. [Genome view]
ENSMUST00000115668; ENSMUSP00000111332; ENSMUSG00000031154; Mus musculus. [Genome view]
GeneID54644.
KEGGmmu:54644.
UCSCuc009smt.1. mouse.

Organism-specific databases

CTD54644.
MGIMGI:1859615. Otud5.

Phylogenomic databases

HOVERGENQ3U2S4.
PhylomeDBQ3U2S4.

Gene expression databases

ArrayExpressQ3U2S4.
BgeeQ3U2S4.
CleanExMM_OTUD5.
GenevestigatorQ3U2S4.

Family and domain databases

InterProIPR003323. OTU.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio311488.
SOURCESearch...

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Entry information

Entry nameOTUD5_MOUSE
AccessionPrimary (citable) accession number: Q3U2S4
Secondary accession number(s): Q3UE33 expand/collapse secondary AC list , Q91YL5, Q9CV50, Q9JIG6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: January 19, 2010
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents