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Q3U2S4

- OTUD5_MOUSE

UniProt

Q3U2S4 - OTUD5_MOUSE

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Protein

OTU domain-containing protein 5

Gene

Otud5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Inhibited by N-ethyl-maleimide (NEM).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211Sequence Analysis
Active sitei224 – 2241NucleophileBy similarity
Active sitei329 – 3291By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. protein K48-linked deubiquitination Source: UniProtKB
  2. protein K63-linked deubiquitination Source: UniProtKB
  3. response to lipopolysaccharide Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_198532. Negative regulators of RIG-I/MDA5 signaling.

Protein family/group databases

MEROPSiC85.001.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Short name:
DUBA
Gene namesi
Name:Otud5
Synonyms:DXImx46e, Sfc7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1859615. Otud5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566OTU domain-containing protein 5PRO_0000278224Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641PhosphoserineBy similarity
Modified residuei165 – 1651PhosphoserineBy similarity
Modified residuei175 – 1751PhosphotyrosineBy similarity
Modified residuei177 – 1771PhosphoserineBy similarity
Modified residuei447 – 4471PhosphoserineBy similarity
Modified residuei502 – 5021PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylation at Ser-177 is required for deubiquitinating activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3U2S4.
PRIDEiQ3U2S4.

PTM databases

PhosphoSiteiQ3U2S4.

Expressioni

Gene expression databases

BgeeiQ3U2S4.
CleanExiMM_OTUD5.
ExpressionAtlasiQ3U2S4. baseline and differential.
GenevestigatoriQ3U2S4.

Interactioni

Subunit structurei

Interacts with TRAF3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000111331.

Structurei

3D structure databases

ProteinModelPortaliQ3U2S4.
SMRiQ3U2S4. Positions 173-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini213 – 336124OTUPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 2247Cys-loopBy similarity
Regioni273 – 28311Variable-loopBy similarityAdd
BLAST
Regioni324 – 3296His-loopBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 113109Pro-richAdd
BLAST
Compositional biasi33 – 174142Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C85 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG286112.
GeneTreeiENSGT00510000048473.
HOGENOMiHOG000231360.
HOVERGENiHBG060214.
InParanoidiQ3U2S4.
KOiK12655.
OrthoDBiEOG77Q4X0.
PhylomeDBiQ3U2S4.
TreeFamiTF326812.

Family and domain databases

InterProiIPR003323. OTU.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3U2S4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTILPKKKPP PPDADPANEP PPPGPLPPAP RRGAGVGVGG GGTGVGGGER
60 70 80 90 100
DRDSGVVGAR PRASPPPQGP LPGPPGALHR WALAVPPGAV AGPRPQQASP
110 120 130 140 150
PPCGGPGGPG GGPGDALGAT TAGVGAAGVV VGVGGTVGVG GCCSGPGHSK
160 170 180 190 200
RRRQAPGVGA VGGASPEREE VGAGYNSEDE YEAAAARIEA MDPATVEQQE
210 220 230 240 250
HWFEKALRDK KGFIIKQMKE DGACLFRAVA DQVYGDQDMH EVVRKHCMDY
260 270 280 290 300
LMKNADYFSN YVTEDFTTYI NRKRKNNCHG NHIEMQAMAE MYNRPVEVYQ
310 320 330 340 350
YSTEPINTFH GIHQNEDEPI RVSYHRNIHY NSVVNPNKAT IGVGLGLPSF
360 370 380 390 400
KPGFAEQSLM KNAIKTSEES WIEQQMLEDK KRATDWEATN EAIEEQVARE
410 420 430 440 450
SYLQWLRDQE KQARQVRGPS QPRKASATCS SATAAASSGL EEWTSRSPRQ
460 470 480 490 500
RSSASSPEHP ELHAELGIKP PSPGTVLALA KPPSPCAPGT SSQFSAGGDR
510 520 530 540 550
ATSPLVSLYP ALECRALIQQ MSPSAFGLND WDDDEILASV LAVSQQEYLD
560
SMKKNKVHRE PPPDKS
Length:566
Mass (Da):60,306
Last modified:February 20, 2007 - v2
Checksum:i89AAD0FF58792EFB
GO
Isoform 2 (identifier: Q3U2S4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-160: Missing.

Show »
Length:523
Mass (Da):56,548
Checksum:i5055D6F15219CAFC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti199 – 1991Missing in AAF66952. (PubMed:15489334)Curated
Sequence conflicti306 – 3061I → F in AAF66952. (PubMed:15489334)Curated
Sequence conflicti308 – 3081T → I in BAE33066. (PubMed:16141072)Curated
Sequence conflicti436 – 4361A → V in AAF66952. (PubMed:15489334)Curated
Sequence conflicti526 – 5261F → FA in BAE33066. (PubMed:16141072)Curated
Sequence conflicti532 – 5321D → Y in BAE29078. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei118 – 16043Missing in isoform 2. 1 PublicationVSP_023196Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK009620 mRNA. Translation: BAB26396.1.
AK149777 mRNA. Translation: BAE29078.1.
AK155131 mRNA. Translation: BAE33066.1.
AK171874 mRNA. Translation: BAE42712.1.
BC016529 mRNA. Translation: AAH16529.1.
BC051111 mRNA. Translation: AAH51111.1.
AF229642 mRNA. Translation: AAF66952.1.
AL671995 Genomic DNA. Translation: CAM13606.1.
AL671978 Genomic DNA. Translation: CAM24561.1.
CCDSiCCDS29975.1. [Q3U2S4-1]
CCDS72335.1. [Q3U2S4-2]
RefSeqiNP_001277466.1. NM_001290537.1. [Q3U2S4-2]
NP_613070.2. NM_138604.3. [Q3U2S4-1]
UniGeneiMm.142827.

Genome annotation databases

EnsembliENSMUST00000033494; ENSMUSP00000033494; ENSMUSG00000031154. [Q3U2S4-1]
ENSMUST00000115668; ENSMUSP00000111332; ENSMUSG00000031154. [Q3U2S4-2]
GeneIDi54644.
KEGGimmu:54644.
UCSCiuc009smu.1. mouse. [Q3U2S4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK009620 mRNA. Translation: BAB26396.1 .
AK149777 mRNA. Translation: BAE29078.1 .
AK155131 mRNA. Translation: BAE33066.1 .
AK171874 mRNA. Translation: BAE42712.1 .
BC016529 mRNA. Translation: AAH16529.1 .
BC051111 mRNA. Translation: AAH51111.1 .
AF229642 mRNA. Translation: AAF66952.1 .
AL671995 Genomic DNA. Translation: CAM13606.1 .
AL671978 Genomic DNA. Translation: CAM24561.1 .
CCDSi CCDS29975.1. [Q3U2S4-1 ]
CCDS72335.1. [Q3U2S4-2 ]
RefSeqi NP_001277466.1. NM_001290537.1. [Q3U2S4-2 ]
NP_613070.2. NM_138604.3. [Q3U2S4-1 ]
UniGenei Mm.142827.

3D structure databases

ProteinModelPortali Q3U2S4.
SMRi Q3U2S4. Positions 173-336.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000111331.

Protein family/group databases

MEROPSi C85.001.

PTM databases

PhosphoSitei Q3U2S4.

Proteomic databases

PaxDbi Q3U2S4.
PRIDEi Q3U2S4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033494 ; ENSMUSP00000033494 ; ENSMUSG00000031154 . [Q3U2S4-1 ]
ENSMUST00000115668 ; ENSMUSP00000111332 ; ENSMUSG00000031154 . [Q3U2S4-2 ]
GeneIDi 54644.
KEGGi mmu:54644.
UCSCi uc009smu.1. mouse. [Q3U2S4-1 ]

Organism-specific databases

CTDi 55593.
MGIi MGI:1859615. Otud5.

Phylogenomic databases

eggNOGi NOG286112.
GeneTreei ENSGT00510000048473.
HOGENOMi HOG000231360.
HOVERGENi HBG060214.
InParanoidi Q3U2S4.
KOi K12655.
OrthoDBi EOG77Q4X0.
PhylomeDBi Q3U2S4.
TreeFami TF326812.

Enzyme and pathway databases

Reactomei REACT_198532. Negative regulators of RIG-I/MDA5 signaling.

Miscellaneous databases

ChiTaRSi OTUD5. mouse.
NextBioi 311488.
PROi Q3U2S4.
SOURCEi Search...

Gene expression databases

Bgeei Q3U2S4.
CleanExi MM_OTUD5.
ExpressionAtlasi Q3U2S4. baseline and differential.
Genevestigatori Q3U2S4.

Family and domain databases

InterProi IPR003323. OTU.
[Graphical view ]
Pfami PF02338. OTU. 1 hit.
[Graphical view ]
PROSITEi PS50802. OTU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Spleen and Tongue.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "A transcript map of a 2-Mb BAC contig in the proximal portion of the mouse X chromosome and regional mapping of the scurfy mutation."
    Means G.D., Toy D.Y., Baum P.R., Derry J.M.J.
    Genomics 65:213-223(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-566 (ISOFORMS 1/2).

Entry informationi

Entry nameiOTUD5_MOUSE
AccessioniPrimary (citable) accession number: Q3U2S4
Secondary accession number(s): Q3UE33
, Q91YL5, Q9CV50, Q9JIG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: October 29, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3