Reviewed,
UniProtKB/Swiss-Prot Q3U2S4 (OTUD5_MOUSE)
Last modified
January 19, 2010.
Version 35.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: OTU domain-containing protein 5 EC=3.1.2.15 Alternative name(s): Deubiquinating enzyme A Short name=DUBA | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 566 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Negative regulator of innate immune system. Has deubiquitinating activity that is directed towards 'Lys-63'-linked polyubiquitin chains. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production By similarity. |
| Catalytic activity | Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. |
| Enzyme regulation | Inhibited by N-ethyl-maleimide (NEM) By similarity. |
| Subunit structure | Interacts with TRAF3 By similarity. |
| Sequence similarities | Belongs to the peptidase C85 family. Contains 1 OTU domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Ubl conjugation pathway |
| Coding sequence diversity | Alternative splicing |
| Molecular function | Hydrolase Protease Thiol protease |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q3U2S4-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q3U2S4-2) The sequence of this isoform differs from the canonical sequence as follows: 118-160: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 566 | 566 | OTU domain-containing protein 5 | PRO_0000278224 | |||||
Regions | |||||||||
| Domain | 213 – 336 | 124 | OTU | ||||||
| Compositional bias | 5 – 113 | 109 | Pro-rich | ||||||
| Compositional bias | 33 – 174 | 142 | Gly-rich | ||||||
Sites | |||||||||
| Active site | 221 | 1 | Potential | ||||||
| Active site | 224 | 1 | Potential | ||||||
| Active site | 329 | 1 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 64 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 165 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 175 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 177 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 447 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 502 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 503 | 1 | Phosphoserine Ref.5 | ||||||
Natural variations | |||||||||
| Alternative sequence | 118 – 160 | 43 | Missing in isoform 2. | VSP_023196 | |||||
Experimental info | |||||||||
| Sequence conflict | 199 | 1 | Missing in AAF66952. Ref.3 | ||||||
| Sequence conflict | 306 | 1 | I → F in AAF66952. Ref.3 | ||||||
| Sequence conflict | 308 | 1 | T → I in BAE33066. Ref.1 | ||||||
| Sequence conflict | 436 | 1 | A → V in AAF66952. Ref.3 | ||||||
| Sequence conflict | 526 | 1 | F → FA in BAE33066. Ref.1 | ||||||
| Sequence conflict | 532 | 1 | D → Y in BAE29078. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J and NOD. Tissue: Bone marrow, Spleen and Tongue. |
| [2] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary gland. |
| [4] | "A transcript map of a 2-Mb BAC contig in the proximal portion of the mouse X chromosome and regional mapping of the scurfy mutation." Means G.D., Toy D.Y., Baum P.R., Derry J.M.J. Genomics 65:213-223(2000) [PubMed: 10857745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-566 (ISOFORMS 1/2). |
| [5] | "Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-503, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK009620 mRNA. Translation: BAB26396.1. AK149777 mRNA. Translation: BAE29078.1. AK155131 mRNA. Translation: BAE33066.1. AK171874 mRNA. Translation: BAE42712.1. BC016529 mRNA. Translation: AAH16529.1. BC051111 mRNA. Translation: AAH51111.1. AF229642 mRNA. Translation: AAF66952.1. AL671995 Genomic DNA. Translation: CAM13606.1. AL671978 Genomic DNA. Translation: CAM24561.1. |
| IPI | IPI00775941. IPI00776134. |
| RefSeq | NP_613070.2. |
| UniGene | Mm.142827 |
3D structure databases | |
| SMR | Q3U2S4. Positions 208-335. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q3U2S4. |
Proteomic databases | |
| PRIDE | Q3U2S4. |
Genome annotation databases | |
| Ensembl | ENSMUST00000033494; ENSMUSP00000033494; ENSMUSG00000031154; Mus musculus. [Genome view] ENSMUST00000115668; ENSMUSP00000111332; ENSMUSG00000031154; Mus musculus. [Genome view] |
| GeneID | 54644. |
| KEGG | mmu:54644. |
| UCSC | uc009smt.1. mouse. |
Organism-specific databases | |
| CTD | 54644. |
| MGI | MGI:1859615. Otud5. |
Phylogenomic databases | |
| HOVERGEN | Q3U2S4. |
| PhylomeDB | Q3U2S4. |
Gene expression databases | |
| ArrayExpress | Q3U2S4. |
| Bgee | Q3U2S4. |
| CleanEx | MM_OTUD5. |
| Genevestigator | Q3U2S4. |
Family and domain databases | |
| InterPro | IPR003323. OTU. [Graphical view] |
| Pfam | PF02338. OTU. 1 hit. [Graphical view] |
| PROSITE | PS50802. OTU. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 311488. |
| SOURCE | Search... |
Entry information
| Entry name | OTUD5_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q3U2S4 Secondary accession number(s): Q3UE33 Q9JIG6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


