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Protein

OTU domain-containing protein 5

Gene

Otud5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Inhibited by N-ethyl-maleimide (NEM).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211Sequence Analysis
Active sitei224 – 2241NucleophileBy similarity
Active sitei329 – 3291By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiREACT_289760. Negative regulators of RIG-I/MDA5 signaling.

Protein family/group databases

MEROPSiC85.001.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme A
Short name:
DUBA
Gene namesi
Name:Otud5
Synonyms:DXImx46e, Sfc7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1859615. Otud5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566OTU domain-containing protein 5PRO_0000278224Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641PhosphoserineBy similarity
Modified residuei165 – 1651PhosphoserineBy similarity
Modified residuei175 – 1751PhosphotyrosineBy similarity
Modified residuei177 – 1771PhosphoserineBy similarity
Modified residuei447 – 4471PhosphoserineBy similarity
Modified residuei502 – 5021PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylation at Ser-177 is required for deubiquitinating activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3U2S4.
PRIDEiQ3U2S4.

PTM databases

PhosphoSiteiQ3U2S4.

Expressioni

Gene expression databases

BgeeiQ3U2S4.
CleanExiMM_OTUD5.
ExpressionAtlasiQ3U2S4. baseline and differential.
GenevisibleiQ3U2S4. MM.

Interactioni

Subunit structurei

Interacts with TRAF3.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033494.

Structurei

3D structure databases

ProteinModelPortaliQ3U2S4.
SMRiQ3U2S4. Positions 173-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini213 – 336124OTUPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 2247Cys-loopBy similarity
Regioni273 – 28311Variable-loopBy similarityAdd
BLAST
Regioni324 – 3296His-loopBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 113109Pro-richAdd
BLAST
Compositional biasi33 – 174142Gly-richAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C85 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG286112.
GeneTreeiENSGT00510000048473.
HOGENOMiHOG000231360.
HOVERGENiHBG060214.
InParanoidiQ3U2S4.
KOiK12655.
OrthoDBiEOG77Q4X0.
PhylomeDBiQ3U2S4.
TreeFamiTF326812.

Family and domain databases

InterProiIPR003323. OTU_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3U2S4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTILPKKKPP PPDADPANEP PPPGPLPPAP RRGAGVGVGG GGTGVGGGER
60 70 80 90 100
DRDSGVVGAR PRASPPPQGP LPGPPGALHR WALAVPPGAV AGPRPQQASP
110 120 130 140 150
PPCGGPGGPG GGPGDALGAT TAGVGAAGVV VGVGGTVGVG GCCSGPGHSK
160 170 180 190 200
RRRQAPGVGA VGGASPEREE VGAGYNSEDE YEAAAARIEA MDPATVEQQE
210 220 230 240 250
HWFEKALRDK KGFIIKQMKE DGACLFRAVA DQVYGDQDMH EVVRKHCMDY
260 270 280 290 300
LMKNADYFSN YVTEDFTTYI NRKRKNNCHG NHIEMQAMAE MYNRPVEVYQ
310 320 330 340 350
YSTEPINTFH GIHQNEDEPI RVSYHRNIHY NSVVNPNKAT IGVGLGLPSF
360 370 380 390 400
KPGFAEQSLM KNAIKTSEES WIEQQMLEDK KRATDWEATN EAIEEQVARE
410 420 430 440 450
SYLQWLRDQE KQARQVRGPS QPRKASATCS SATAAASSGL EEWTSRSPRQ
460 470 480 490 500
RSSASSPEHP ELHAELGIKP PSPGTVLALA KPPSPCAPGT SSQFSAGGDR
510 520 530 540 550
ATSPLVSLYP ALECRALIQQ MSPSAFGLND WDDDEILASV LAVSQQEYLD
560
SMKKNKVHRE PPPDKS
Length:566
Mass (Da):60,306
Last modified:February 20, 2007 - v2
Checksum:i89AAD0FF58792EFB
GO
Isoform 2 (identifier: Q3U2S4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-160: Missing.

Show »
Length:523
Mass (Da):56,548
Checksum:i5055D6F15219CAFC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti199 – 1991Missing in AAF66952 (PubMed:15489334).Curated
Sequence conflicti306 – 3061I → F in AAF66952 (PubMed:15489334).Curated
Sequence conflicti308 – 3081T → I in BAE33066 (PubMed:16141072).Curated
Sequence conflicti436 – 4361A → V in AAF66952 (PubMed:15489334).Curated
Sequence conflicti526 – 5261F → FA in BAE33066 (PubMed:16141072).Curated
Sequence conflicti532 – 5321D → Y in BAE29078 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei118 – 16043Missing in isoform 2. 1 PublicationVSP_023196Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009620 mRNA. Translation: BAB26396.1.
AK149777 mRNA. Translation: BAE29078.1.
AK155131 mRNA. Translation: BAE33066.1.
AK171874 mRNA. Translation: BAE42712.1.
BC016529 mRNA. Translation: AAH16529.1.
BC051111 mRNA. Translation: AAH51111.1.
AF229642 mRNA. Translation: AAF66952.1.
AL671995 Genomic DNA. Translation: CAM13606.1.
AL671978 Genomic DNA. Translation: CAM24561.1.
CCDSiCCDS29975.1. [Q3U2S4-1]
CCDS72335.1. [Q3U2S4-2]
RefSeqiNP_001277466.1. NM_001290537.1. [Q3U2S4-2]
NP_613070.2. NM_138604.3. [Q3U2S4-1]
UniGeneiMm.142827.

Genome annotation databases

EnsembliENSMUST00000033494; ENSMUSP00000033494; ENSMUSG00000031154. [Q3U2S4-1]
ENSMUST00000115668; ENSMUSP00000111332; ENSMUSG00000031154. [Q3U2S4-2]
GeneIDi54644.
KEGGimmu:54644.
UCSCiuc009smu.1. mouse. [Q3U2S4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK009620 mRNA. Translation: BAB26396.1.
AK149777 mRNA. Translation: BAE29078.1.
AK155131 mRNA. Translation: BAE33066.1.
AK171874 mRNA. Translation: BAE42712.1.
BC016529 mRNA. Translation: AAH16529.1.
BC051111 mRNA. Translation: AAH51111.1.
AF229642 mRNA. Translation: AAF66952.1.
AL671995 Genomic DNA. Translation: CAM13606.1.
AL671978 Genomic DNA. Translation: CAM24561.1.
CCDSiCCDS29975.1. [Q3U2S4-1]
CCDS72335.1. [Q3U2S4-2]
RefSeqiNP_001277466.1. NM_001290537.1. [Q3U2S4-2]
NP_613070.2. NM_138604.3. [Q3U2S4-1]
UniGeneiMm.142827.

3D structure databases

ProteinModelPortaliQ3U2S4.
SMRiQ3U2S4. Positions 173-336.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033494.

Protein family/group databases

MEROPSiC85.001.

PTM databases

PhosphoSiteiQ3U2S4.

Proteomic databases

PaxDbiQ3U2S4.
PRIDEiQ3U2S4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033494; ENSMUSP00000033494; ENSMUSG00000031154. [Q3U2S4-1]
ENSMUST00000115668; ENSMUSP00000111332; ENSMUSG00000031154. [Q3U2S4-2]
GeneIDi54644.
KEGGimmu:54644.
UCSCiuc009smu.1. mouse. [Q3U2S4-1]

Organism-specific databases

CTDi55593.
MGIiMGI:1859615. Otud5.

Phylogenomic databases

eggNOGiNOG286112.
GeneTreeiENSGT00510000048473.
HOGENOMiHOG000231360.
HOVERGENiHBG060214.
InParanoidiQ3U2S4.
KOiK12655.
OrthoDBiEOG77Q4X0.
PhylomeDBiQ3U2S4.
TreeFamiTF326812.

Enzyme and pathway databases

ReactomeiREACT_289760. Negative regulators of RIG-I/MDA5 signaling.

Miscellaneous databases

ChiTaRSiOtud5. mouse.
NextBioi311488.
PROiQ3U2S4.
SOURCEiSearch...

Gene expression databases

BgeeiQ3U2S4.
CleanExiMM_OTUD5.
ExpressionAtlasiQ3U2S4. baseline and differential.
GenevisibleiQ3U2S4. MM.

Family and domain databases

InterProiIPR003323. OTU_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Spleen and Tongue.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "A transcript map of a 2-Mb BAC contig in the proximal portion of the mouse X chromosome and regional mapping of the scurfy mutation."
    Means G.D., Toy D.Y., Baum P.R., Derry J.M.J.
    Genomics 65:213-223(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-566 (ISOFORMS 1/2).

Entry informationi

Entry nameiOTUD5_MOUSE
AccessioniPrimary (citable) accession number: Q3U2S4
Secondary accession number(s): Q3UE33
, Q91YL5, Q9CV50, Q9JIG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: June 24, 2015
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.