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Q3U2S4

- OTUD5_MOUSE

UniProt

Q3U2S4 - OTUD5_MOUSE

Protein

OTU domain-containing protein 5

Gene

Otud5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (20 Feb 2007)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    Inhibited by N-ethyl-maleimide (NEM).By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei221 – 2211Sequence Analysis
    Active sitei224 – 2241NucleophileBy similarity
    Active sitei329 – 3291By similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. protein K48-linked deubiquitination Source: UniProtKB
    2. protein K63-linked deubiquitination Source: UniProtKB
    3. response to lipopolysaccharide Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_198532. Negative regulators of RIG-I/MDA5 signaling.

    Protein family/group databases

    MEROPSiC85.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    OTU domain-containing protein 5 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme A
    Short name:
    DUBA
    Gene namesi
    Name:Otud5
    Synonyms:DXImx46e, Sfc7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1859615. Otud5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 566566OTU domain-containing protein 5PRO_0000278224Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei64 – 641PhosphoserineBy similarity
    Modified residuei165 – 1651PhosphoserineBy similarity
    Modified residuei175 – 1751PhosphotyrosineBy similarity
    Modified residuei177 – 1771PhosphoserineBy similarity
    Modified residuei447 – 4471PhosphoserineBy similarity
    Modified residuei502 – 5021PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-177 is required for deubiquitinating activity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ3U2S4.
    PRIDEiQ3U2S4.

    PTM databases

    PhosphoSiteiQ3U2S4.

    Expressioni

    Gene expression databases

    BgeeiQ3U2S4.
    CleanExiMM_OTUD5.
    GenevestigatoriQ3U2S4.

    Interactioni

    Subunit structurei

    Interacts with TRAF3.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000111331.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3U2S4.
    SMRiQ3U2S4. Positions 173-336.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini213 – 336124OTUPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2247Cys-loopBy similarity
    Regioni273 – 28311Variable-loopBy similarityAdd
    BLAST
    Regioni324 – 3296His-loopBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi5 – 113109Pro-richAdd
    BLAST
    Compositional biasi33 – 174142Gly-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C85 family.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG286112.
    GeneTreeiENSGT00510000048473.
    HOGENOMiHOG000231360.
    HOVERGENiHBG060214.
    KOiK12655.
    OrthoDBiEOG77Q4X0.
    PhylomeDBiQ3U2S4.
    TreeFamiTF326812.

    Family and domain databases

    InterProiIPR003323. OTU.
    [Graphical view]
    PfamiPF02338. OTU. 1 hit.
    [Graphical view]
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3U2S4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTILPKKKPP PPDADPANEP PPPGPLPPAP RRGAGVGVGG GGTGVGGGER    50
    DRDSGVVGAR PRASPPPQGP LPGPPGALHR WALAVPPGAV AGPRPQQASP 100
    PPCGGPGGPG GGPGDALGAT TAGVGAAGVV VGVGGTVGVG GCCSGPGHSK 150
    RRRQAPGVGA VGGASPEREE VGAGYNSEDE YEAAAARIEA MDPATVEQQE 200
    HWFEKALRDK KGFIIKQMKE DGACLFRAVA DQVYGDQDMH EVVRKHCMDY 250
    LMKNADYFSN YVTEDFTTYI NRKRKNNCHG NHIEMQAMAE MYNRPVEVYQ 300
    YSTEPINTFH GIHQNEDEPI RVSYHRNIHY NSVVNPNKAT IGVGLGLPSF 350
    KPGFAEQSLM KNAIKTSEES WIEQQMLEDK KRATDWEATN EAIEEQVARE 400
    SYLQWLRDQE KQARQVRGPS QPRKASATCS SATAAASSGL EEWTSRSPRQ 450
    RSSASSPEHP ELHAELGIKP PSPGTVLALA KPPSPCAPGT SSQFSAGGDR 500
    ATSPLVSLYP ALECRALIQQ MSPSAFGLND WDDDEILASV LAVSQQEYLD 550
    SMKKNKVHRE PPPDKS 566
    Length:566
    Mass (Da):60,306
    Last modified:February 20, 2007 - v2
    Checksum:i89AAD0FF58792EFB
    GO
    Isoform 2 (identifier: Q3U2S4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         118-160: Missing.

    Show »
    Length:523
    Mass (Da):56,548
    Checksum:i5055D6F15219CAFC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti199 – 1991Missing in AAF66952. (PubMed:15489334)Curated
    Sequence conflicti306 – 3061I → F in AAF66952. (PubMed:15489334)Curated
    Sequence conflicti308 – 3081T → I in BAE33066. (PubMed:16141072)Curated
    Sequence conflicti436 – 4361A → V in AAF66952. (PubMed:15489334)Curated
    Sequence conflicti526 – 5261F → FA in BAE33066. (PubMed:16141072)Curated
    Sequence conflicti532 – 5321D → Y in BAE29078. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei118 – 16043Missing in isoform 2. 1 PublicationVSP_023196Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK009620 mRNA. Translation: BAB26396.1.
    AK149777 mRNA. Translation: BAE29078.1.
    AK155131 mRNA. Translation: BAE33066.1.
    AK171874 mRNA. Translation: BAE42712.1.
    BC016529 mRNA. Translation: AAH16529.1.
    BC051111 mRNA. Translation: AAH51111.1.
    AF229642 mRNA. Translation: AAF66952.1.
    AL671995 Genomic DNA. Translation: CAM13606.1.
    AL671978 Genomic DNA. Translation: CAM24561.1.
    CCDSiCCDS29975.1. [Q3U2S4-1]
    CCDS72335.1. [Q3U2S4-2]
    RefSeqiNP_001277466.1. NM_001290537.1. [Q3U2S4-2]
    NP_613070.2. NM_138604.3. [Q3U2S4-1]
    UniGeneiMm.142827.

    Genome annotation databases

    EnsembliENSMUST00000033494; ENSMUSP00000033494; ENSMUSG00000031154. [Q3U2S4-1]
    ENSMUST00000115668; ENSMUSP00000111332; ENSMUSG00000031154. [Q3U2S4-2]
    GeneIDi54644.
    KEGGimmu:54644.
    UCSCiuc009smu.1. mouse. [Q3U2S4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK009620 mRNA. Translation: BAB26396.1 .
    AK149777 mRNA. Translation: BAE29078.1 .
    AK155131 mRNA. Translation: BAE33066.1 .
    AK171874 mRNA. Translation: BAE42712.1 .
    BC016529 mRNA. Translation: AAH16529.1 .
    BC051111 mRNA. Translation: AAH51111.1 .
    AF229642 mRNA. Translation: AAF66952.1 .
    AL671995 Genomic DNA. Translation: CAM13606.1 .
    AL671978 Genomic DNA. Translation: CAM24561.1 .
    CCDSi CCDS29975.1. [Q3U2S4-1 ]
    CCDS72335.1. [Q3U2S4-2 ]
    RefSeqi NP_001277466.1. NM_001290537.1. [Q3U2S4-2 ]
    NP_613070.2. NM_138604.3. [Q3U2S4-1 ]
    UniGenei Mm.142827.

    3D structure databases

    ProteinModelPortali Q3U2S4.
    SMRi Q3U2S4. Positions 173-336.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000111331.

    Protein family/group databases

    MEROPSi C85.001.

    PTM databases

    PhosphoSitei Q3U2S4.

    Proteomic databases

    PaxDbi Q3U2S4.
    PRIDEi Q3U2S4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033494 ; ENSMUSP00000033494 ; ENSMUSG00000031154 . [Q3U2S4-1 ]
    ENSMUST00000115668 ; ENSMUSP00000111332 ; ENSMUSG00000031154 . [Q3U2S4-2 ]
    GeneIDi 54644.
    KEGGi mmu:54644.
    UCSCi uc009smu.1. mouse. [Q3U2S4-1 ]

    Organism-specific databases

    CTDi 55593.
    MGIi MGI:1859615. Otud5.

    Phylogenomic databases

    eggNOGi NOG286112.
    GeneTreei ENSGT00510000048473.
    HOGENOMi HOG000231360.
    HOVERGENi HBG060214.
    KOi K12655.
    OrthoDBi EOG77Q4X0.
    PhylomeDBi Q3U2S4.
    TreeFami TF326812.

    Enzyme and pathway databases

    Reactomei REACT_198532. Negative regulators of RIG-I/MDA5 signaling.

    Miscellaneous databases

    ChiTaRSi OTUD5. mouse.
    NextBioi 311488.
    PROi Q3U2S4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3U2S4.
    CleanExi MM_OTUD5.
    Genevestigatori Q3U2S4.

    Family and domain databases

    InterProi IPR003323. OTU.
    [Graphical view ]
    Pfami PF02338. OTU. 1 hit.
    [Graphical view ]
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Spleen and Tongue.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary gland.
    4. "A transcript map of a 2-Mb BAC contig in the proximal portion of the mouse X chromosome and regional mapping of the scurfy mutation."
      Means G.D., Toy D.Y., Baum P.R., Derry J.M.J.
      Genomics 65:213-223(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-566 (ISOFORMS 1/2).

    Entry informationi

    Entry nameiOTUD5_MOUSE
    AccessioniPrimary (citable) accession number: Q3U2S4
    Secondary accession number(s): Q3UE33
    , Q91YL5, Q9CV50, Q9JIG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: February 20, 2007
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3