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Q3U2S4 (OTUD5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
OTU domain-containing protein 5

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme A
Short name=DUBA
Gene names
Name:Otud5
Synonyms:DXImx46e, Sfc7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Can also cleave 'Lys-11'-linked ubiquitin chains (in vitro) By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulation

Inhibited by N-ethyl-maleimide (NEM) By similarity.

Subunit structure

Interacts with TRAF3 By similarity.

Post-translational modification

Phosphorylation at Ser-177 is required for deubiquitinating activity By similarity.

Sequence similarities

Belongs to the peptidase C85 family.

Contains 1 OTU domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3U2S4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3U2S4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     118-160: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566OTU domain-containing protein 5
PRO_0000278224

Regions

Domain213 – 336124OTU
Region218 – 2247Cys-loop By similarity
Region273 – 28311Variable-loop By similarity
Region324 – 3296His-loop By similarity
Compositional bias5 – 113109Pro-rich
Compositional bias33 – 174142Gly-rich

Sites

Active site2211 Potential
Active site2241Nucleophile By similarity
Active site3291 By similarity

Amino acid modifications

Modified residue641Phosphoserine By similarity
Modified residue1651Phosphoserine By similarity
Modified residue1751Phosphotyrosine By similarity
Modified residue1771Phosphoserine By similarity
Modified residue4471Phosphoserine By similarity
Modified residue5021Phosphothreonine By similarity

Natural variations

Alternative sequence118 – 16043Missing in isoform 2.
VSP_023196

Experimental info

Sequence conflict1991Missing in AAF66952. Ref.3
Sequence conflict3061I → F in AAF66952. Ref.3
Sequence conflict3081T → I in BAE33066. Ref.1
Sequence conflict4361A → V in AAF66952. Ref.3
Sequence conflict5261F → FA in BAE33066. Ref.1
Sequence conflict5321D → Y in BAE29078. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 20, 2007. Version 2.
Checksum: 89AAD0FF58792EFB

FASTA56660,306
        10         20         30         40         50         60 
MTILPKKKPP PPDADPANEP PPPGPLPPAP RRGAGVGVGG GGTGVGGGER DRDSGVVGAR 

        70         80         90        100        110        120 
PRASPPPQGP LPGPPGALHR WALAVPPGAV AGPRPQQASP PPCGGPGGPG GGPGDALGAT 

       130        140        150        160        170        180 
TAGVGAAGVV VGVGGTVGVG GCCSGPGHSK RRRQAPGVGA VGGASPEREE VGAGYNSEDE 

       190        200        210        220        230        240 
YEAAAARIEA MDPATVEQQE HWFEKALRDK KGFIIKQMKE DGACLFRAVA DQVYGDQDMH 

       250        260        270        280        290        300 
EVVRKHCMDY LMKNADYFSN YVTEDFTTYI NRKRKNNCHG NHIEMQAMAE MYNRPVEVYQ 

       310        320        330        340        350        360 
YSTEPINTFH GIHQNEDEPI RVSYHRNIHY NSVVNPNKAT IGVGLGLPSF KPGFAEQSLM 

       370        380        390        400        410        420 
KNAIKTSEES WIEQQMLEDK KRATDWEATN EAIEEQVARE SYLQWLRDQE KQARQVRGPS 

       430        440        450        460        470        480 
QPRKASATCS SATAAASSGL EEWTSRSPRQ RSSASSPEHP ELHAELGIKP PSPGTVLALA 

       490        500        510        520        530        540 
KPPSPCAPGT SSQFSAGGDR ATSPLVSLYP ALECRALIQQ MSPSAFGLND WDDDEILASV 

       550        560 
LAVSQQEYLD SMKKNKVHRE PPPDKS 

« Hide

Isoform 2 [UniParc].

Checksum: 5055D6F15219CAFC
Show »

FASTA52356,548

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Bone marrow, Spleen and Tongue.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary gland.
[4]"A transcript map of a 2-Mb BAC contig in the proximal portion of the mouse X chromosome and regional mapping of the scurfy mutation."
Means G.D., Toy D.Y., Baum P.R., Derry J.M.J.
Genomics 65:213-223(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 191-566 (ISOFORMS 1/2).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK009620 mRNA. Translation: BAB26396.1.
AK149777 mRNA. Translation: BAE29078.1.
AK155131 mRNA. Translation: BAE33066.1.
AK171874 mRNA. Translation: BAE42712.1.
BC016529 mRNA. Translation: AAH16529.1.
BC051111 mRNA. Translation: AAH51111.1.
AF229642 mRNA. Translation: AAF66952.1.
AL671995 Genomic DNA. Translation: CAM13606.1.
AL671978 Genomic DNA. Translation: CAM24561.1.
RefSeqNP_613070.2. NM_138604.2.
XP_006527731.1. XM_006527668.1.
UniGeneMm.142827.

3D structure databases

ProteinModelPortalQ3U2S4.
SMRQ3U2S4. Positions 173-336.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000111331.

Protein family/group databases

MEROPSC85.001.

PTM databases

PhosphoSiteQ3U2S4.

Proteomic databases

PaxDbQ3U2S4.
PRIDEQ3U2S4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033494; ENSMUSP00000033494; ENSMUSG00000031154. [Q3U2S4-1]
ENSMUST00000115668; ENSMUSP00000111332; ENSMUSG00000031154. [Q3U2S4-2]
GeneID54644.
KEGGmmu:54644.
UCSCuc009smu.1. mouse. [Q3U2S4-1]

Organism-specific databases

CTD55593.
MGIMGI:1859615. Otud5.

Phylogenomic databases

eggNOGNOG286112.
GeneTreeENSGT00510000048473.
HOGENOMHOG000231360.
HOVERGENHBG060214.
KOK12655.
OrthoDBEOG77Q4X0.
TreeFamTF326812.

Gene expression databases

ArrayExpressQ3U2S4.
BgeeQ3U2S4.
CleanExMM_OTUD5.
GenevestigatorQ3U2S4.

Family and domain databases

InterProIPR003323. OTU.
[Graphical view]
PfamPF02338. OTU. 1 hit.
[Graphical view]
PROSITEPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSOTUD5. mouse.
NextBio311488.
PROQ3U2S4.
SOURCESearch...

Entry information

Entry nameOTUD5_MOUSE
AccessionPrimary (citable) accession number: Q3U2S4
Secondary accession number(s): Q3UE33 expand/collapse secondary AC list , Q91YL5, Q9CV50, Q9JIG6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: February 20, 2007
Last modified: March 19, 2014
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot