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Protein

Microtubule-associated serine/threonine-protein kinase 3

Gene

Mast3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei418 – 4181ATPPROSITE-ProRule annotation
Active sitei512 – 5121Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi395 – 4039ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated serine/threonine-protein kinase 3 (EC:2.7.11.1)
Gene namesi
Name:Mast3
Synonyms:Kiaa0561
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2683541. Mast3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13211321Microtubule-associated serine/threonine-protein kinase 3PRO_0000277823Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei107 – 1071PhosphoserineCombined sources
Modified residuei159 – 1591PhosphoserineCombined sources
Modified residuei168 – 1681PhosphoserineBy similarity
Modified residuei702 – 7021PhosphoserineCombined sources
Modified residuei732 – 7321PhosphoserineCombined sources
Modified residuei750 – 7501PhosphoserineCombined sources
Modified residuei795 – 7951PhosphoserineBy similarity
Modified residuei803 – 8031PhosphoserineBy similarity
Modified residuei813 – 8131PhosphoserineBy similarity
Modified residuei814 – 8141PhosphoserineBy similarity
Modified residuei1237 – 12371PhosphoserineCombined sources
Modified residuei1289 – 12891PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3U214.
MaxQBiQ3U214.
PaxDbiQ3U214.
PRIDEiQ3U214.

PTM databases

iPTMnetiQ3U214.
PhosphoSiteiQ3U214.

Expressioni

Gene expression databases

CleanExiMM_MAST3.

Interactioni

Subunit structurei

Interacts with PTEN.By similarity

Protein-protein interaction databases

BioGridi244925. 6 interactions.
STRINGi10090.ENSMUSP00000128703.

Structurei

3D structure databases

ProteinModelPortaliQ3U214.
SMRiQ3U214. Positions 203-303, 964-1060.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini389 – 662274Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini663 – 73472AGC-kinase C-terminalAdd
BLAST
Domaini966 – 105489PDZPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi18 – 131114Ser-richAdd
BLAST
Compositional biasi836 – 8416Poly-Pro
Compositional biasi1102 – 117574Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0606. Eukaryota.
ENOG410XPWX. LUCA.
HOGENOMiHOG000046662.
HOVERGENiHBG052414.
InParanoidiQ3U214.
KOiK08789.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3U214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSRREKLLI PALTLDLSPS SQSPCLLSPG SPCSPCSPSL GLQPWSCRSG
60 70 80 90 100
NRKSLVVGTP SPTLSRPLSP LSVPTAGNSP LDSPRNFSAA AAISFPFARR
110 120 130 140 150
ADGRRWSLAS LPSSGYGTNT PSSTVSSSSS SRERLHQLPF QPTADELRFL
160 170 180 190 200
SKHFRSSESV VDEDGGRSPR LRPRSRSLSP GRTSGTFDNE IVMMNHVYRE
210 220 230 240 250
RFPKATAQME GRLQDFLAAF APGDRLALAD GVLGFIHHQI VELARDCLAK
260 270 280 290 300
SGEALVTSRY FLEMQDKLER LLQDAHERSD SAEVGFIVQL VRKLLIIISR
310 320 330 340 350
PARLLECLEF DPEEFYHLLE AAEGQAREDQ GVKTDLPRYI IRQLGLAKDP
360 370 380 390 400
LEEIQPLNDL DESQPPAPGS PESRGLGGPS RRKPCESDFE TIKLISNGAY
410 420 430 440 450
GAVYLVRHRD TRQRFAIKKI NKQNLILRNQ IQQVFVERDI LTFAENPFVV
460 470 480 490 500
GMFCSFETRR HLCMVMEYVE GGDCATLLKN MGPLPVDMAR MYFAETVLAL
510 520 530 540 550
EYLHNYGIVH RDLKPDNLLI TSLGHIKLTD FGLSKIGLMS MATNLYEGHI
560 570 580 590 600
EKDAREFVDK QVCGTPEYIA PEVIFRQGYG KPVDWWAMGV ILYEFLVGCV
610 620 630 640 650
PFFGDTPEEL FGQVVSDEIM WPEGDEALPL DAQDLITRLL RQSPMDRLGT
660 670 680 690 700
GGTHEVKQHP FFLALDWAGL LRHKAEFVPQ LEAEDDTSYF DTRSERYRHL
710 720 730 740 750
GSEDDETNDE ESSTEIPQFS SCSHRFSKVY SSSEFLAVQP TPTFAERSFS
760 770 780 790 800
EDREDGWGQS LGDRGRRLSA DLRLRSWTPA SSCQPSSCQT DRGPSPSLLS
810 820 830 840 850
TISLDVMPKF AFSSEDEGAS SGPADPQKPV FILGEPDPPP PTTPVTPKPC
860 870 880 890 900
NLSADTAVLS HARLRSNSTG ARHSTPRPLD AGRGRRLGGS RDPGPEKPRA
910 920 930 940 950
SPGGSGGRVP KSASVSALSL IITADDGSGG PLMSPLSPRS LSSNPSSRDS
960 970 980 990 1000
SPSRDPSPVC GSLRPPIVIH SSGKKYGFSL RAIRVYMGDS DVYTVHHVVW
1010 1020 1030 1040 1050
SVEEGSPAQE AGLRAGDLIT HINGESVLGL VHMDVVELLL KSGNKISLRT
1060 1070 1080 1090 1100
TALENTSIKV GPARKNVAKG RMARRSKRSR RRETQDRRKS LFKRISKPSS
1110 1120 1130 1140 1150
VLHTSRSFSS GLQHSLSSSE SLPGSPTHSL SPSPTTPCRS PAPDAPTDTA
1160 1170 1180 1190 1200
SPPNVSPSSS SPASPATGHT RPSSLHGLAA KLGPPRHKSG RRKSTSSIPP
1210 1220 1230 1240 1250
SPLACPPVPT PPPRSPSPLP GHIPIPARSP RLRRGQSADK LGLGTSERLD
1260 1270 1280 1290 1300
GDGGRRARGA EAELVVMRRL HLSERRDSFK KQEAVQEVSF DEEPGPPRGV
1310 1320
PKIAVQGAEA TPGTPGHARK D
Length:1,321
Mass (Da):144,174
Last modified:July 27, 2011 - v3
Checksum:i792F7083719CF402
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811G → E in BAE33328 (PubMed:16141072).Curated
Sequence conflicti263 – 2631E → G in BAE33328 (PubMed:16141072).Curated
Sequence conflicti762 – 7665GDRGR → DAWVG in BC024265 (PubMed:15489334).Curated
Sequence conflicti1148 – 11569DTASPPNVS → GGRALSLAL in BC024265 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC162446 Genomic DNA. No translation available.
AK155568 mRNA. Translation: BAE33328.1.
BC024265 mRNA. No translation available.
AK172971 mRNA. Translation: BAD32249.1.
RefSeqiNP_955012.2. NM_199308.2.
UniGeneiMm.250731.

Genome annotation databases

GeneIDi546071.
KEGGimmu:546071.
UCSCiuc009mbq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC162446 Genomic DNA. No translation available.
AK155568 mRNA. Translation: BAE33328.1.
BC024265 mRNA. No translation available.
AK172971 mRNA. Translation: BAD32249.1.
RefSeqiNP_955012.2. NM_199308.2.
UniGeneiMm.250731.

3D structure databases

ProteinModelPortaliQ3U214.
SMRiQ3U214. Positions 203-303, 964-1060.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi244925. 6 interactions.
STRINGi10090.ENSMUSP00000128703.

PTM databases

iPTMnetiQ3U214.
PhosphoSiteiQ3U214.

Proteomic databases

EPDiQ3U214.
MaxQBiQ3U214.
PaxDbiQ3U214.
PRIDEiQ3U214.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi546071.
KEGGimmu:546071.
UCSCiuc009mbq.1. mouse.

Organism-specific databases

CTDi23031.
MGIiMGI:2683541. Mast3.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0606. Eukaryota.
ENOG410XPWX. LUCA.
HOGENOMiHOG000046662.
HOVERGENiHBG052414.
InParanoidiQ3U214.
KOiK08789.

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.

Miscellaneous databases

ChiTaRSiMast3. mouse.
PROiQ3U214.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MAST3.

Family and domain databases

Gene3Di1.20.1480.20. 1 hit.
2.30.42.10. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR015022. MA_Ser/Thr_Kinase_dom.
IPR023142. MAST_pre-PK_dom.
IPR001478. PDZ.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF08926. DUF1908. 1 hit.
PF00595. PDZ. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF140482. SSF140482. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50106. PDZ. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-786.
    Strain: NOD.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 762-1156.
    Tissue: Liver.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 907-1321.
    Tissue: Thymus.
  5. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1237 AND SER-1289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107; SER-159; SER-702; SER-732; SER-750 AND SER-1289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiMAST3_MOUSE
AccessioniPrimary (citable) accession number: Q3U214
Secondary accession number(s): E9QQ28, Q6A047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 93 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.