Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q3U0P1 (PALB2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Partner and localizer of BRCA2
Gene names
Name:Palb2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1104 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity By similarity.

Subunit structure

Homooligomer; dissociated upon DNA damage thus allowing association with BRCA1. Oligomerization is essential for its focal accumulation at DNA breaks. Part of a BRCA complex containing BRCA1, BRCA2 and PALB2. Interacts with BRCA1 and this interaction is essential for its function in HRR. Interacts with RAD51AP1 and MORF4L1/MRG15. Interacts with BRCA2, RAD51C, RAD51 and XRCC3; the interactions are direct and it may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with POLH; the interaction is direct By similarity.

Subcellular location

Nucleus. Note: Colocalizes with BRCA2 in nuclear foci By similarity.

Domain

Interaction with BRCA2 occurs through a hydrophobic pocket at the crossover between WD repeats 4 and 5 By similarity.

The coiled coil domain mediates self-association.

The chromatin-association motif (ChAM) mediates association with chromatin, probably through nucleosome core particles, independently from binding to D loop, ssDNA or dsDNA structures.

Sequence similarities

Contains 7 WD repeats.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3U0P1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3U0P1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     170-532: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q3U0P1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     36-755: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q3U0P1-4)

The sequence of this isoform differs from the canonical sequence as follows:
     533-544: DFELPDEDFGLL → GKSRRRVRLRLM
     545-1104: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11041104Partner and localizer of BRCA2
PRO_0000252392

Regions

Repeat772 – 83362WD 1
Repeat835 – 87945WD 2
Repeat880 – 92748WD 3
Repeat928 – 97043WD 4
Repeat976 – 102752WD 5
Repeat1033 – 107139WD 6
Repeat1073 – 110432WD 7
Region1 – 308308Interaction with BRCA1 By similarity
Region1 – 195195Interaction with RAD51 By similarity
Region1 – 157157Required for its oligomerization and is important for its focal concentration at DNA damage sites By similarity
Region374 – 42451ChAM (Chromatin-association motif); required for chromatin association, mediates nucleosome association By similarity
Region693 – 1104412Required for interaction with POLH and POLH DNA synthesis stimulation By similarity
Region771 – 1104334Interaction with RAD51 and BRCA2 By similarity
Region771 – 1104334Interaction with RAD51, BRCA2 and POLH By similarity
Coiled coil9 – 4840 Potential

Amino acid modifications

Modified residue3641Phosphoserine By similarity

Natural variations

Alternative sequence36 – 755720Missing in isoform 3.
VSP_020928
Alternative sequence170 – 532363Missing in isoform 2.
VSP_020929
Alternative sequence533 – 54412DFELP…DFGLL → GKSRRRVRLRLM in isoform 4.
VSP_020930
Alternative sequence545 – 1104560Missing in isoform 4.
VSP_020931

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: D8E027609707D03D

FASTA1,104119,097
        10         20         30         40         50         60 
MEELSGKPLS YAEKEKLKEK LAFLKKEYSR TLARLQRAKR AEKAKNSKKA IEDGVPQPEA 

        70         80         90        100        110        120 
SSQLSHSESI NKGFPCDTLQ SNHLDEETGE NISQILDVEP QSFNCKQGKE VLHTPRAGDI 

       130        140        150        160        170        180 
QGQLLHSTSS PDGKKEQNTL PGTTKTPWEK SSVSQEKEDY FDTNSLALLG KHRKGQESIS 

       190        200        210        220        230        240 
RKNSRTPVSE KTHLLSLRSQ IPDPPALVTG IGEGILIPPS GKSERGIDTL VRGNTVSAEA 

       250        260        270        280        290        300 
AVPSCTASNS NHSQHLEHTP PKSGCKITTQ GPASSTNLVA QDQKMTIFTV NSVVYKAVRA 

       310        320        330        340        350        360 
HGQLPGSPNS CSVNDLTHSN LPANSTPNSK SLKSPSNTVD ERNEPLQEDE ILGPSKNFNL 

       370        380        390        400        410        420 
AAVSPPSTES QIHSCTMLEG LLFPAEYYVR TTRRMSDCQR KIALEAVIQS HLGVKKKELK 

       430        440        450        460        470        480 
KKTKATKAVV LSSEDTDQSE SGMLDTSTGQ SSSGSLSQKL LSPAEVSSPP GPAGKATTPP 

       490        500        510        520        530        540 
PGRGHRGKRK SARTSTLGHC QLLFPPCAAL AVNRSKGKFT KHKCQNRGVV IHDFELPDED 

       550        560        570        580        590        600 
FGLLKLEKLK SCSEKLIESP DSKNCGERLP REGNHAALEE LQRDSETEGL EEELTVPPGE 

       610        620        630        640        650        660 
AYRPGPTLRR QPGSKDLSSS IVLFTPADTA APNDSGRPPP SLCSPAFPIL GMTPALGSQA 

       670        680        690        700        710        720 
AGETLSTEAA QPCSTSQPPL LGDTNSLVNN SKQCNSSACS PKPDTNLQAS GRQGQPACDS 

       730        740        750        760        770        780 
DSGPQATPLP VESFTFRENQ LCGNACLELH EHSTEQTETA DRPACDNLNP GNLQLVSELK 

       790        800        810        820        830        840 
NPSSSCSVDV SAMWWERAGA KEPCIVTACE DVVSLWKPLN SLQWEKVHTW HFTEVPVLQI 

       850        860        870        880        890        900 
VPVPDVYNLI CVALGSLEIR EIRALLCSSG DDSEKQVLLK SGDIKAMLGL TKRRLVSSTG 

       910        920        930        940        950        960 
TFCNQQIQIM TFADDGSSKD EQLLMPPDET VLTFAEVQGT QEALLGTTTV NSIVIWNLKT 

       970        980        990       1000       1010       1020 
GQLLKKMHID DSYQASVCHG AYSEKGLLFV VVSQPCAKES QALGSPVFQL LVINPKTAQS 

      1030       1040       1050       1060       1070       1080 
VGVLLCSLPQ GQAGRFLEGD VKDHVAAAVL TSGTIAIWDL LLGHCTALLP PVSDQSWSLV 

      1090       1100 
KWSGTDSHLL AGQKDGNIFI YRYF 

« Hide

Isoform 2 [UniParc].

Checksum: A931115C63372E41
Show »

FASTA74180,495
Isoform 3 [UniParc].

Checksum: 36E8A26E61ED1C38
Show »

FASTA38442,138
Isoform 4 [UniParc].

Checksum: 7B49F932BFEDA83A
Show »

FASTA54458,971

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1086 (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Skin and Spleen.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6J and FVB/N-3.
Tissue: Embryonic germ cell and Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK028653 mRNA. Translation: BAC26048.1.
AK156701 mRNA. Translation: BAE33811.1.
BC055302 mRNA. Translation: AAH55302.1.
BC066140 mRNA. Translation: AAH66140.1.
RefSeqNP_001074707.1. NM_001081238.2.
UniGeneMm.38348.

3D structure databases

ProteinModelPortalQ3U0P1.
SMRQ3U0P1. Positions 772-1103.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ3U0P1.

Proteomic databases

PRIDEQ3U0P1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063587; ENSMUSP00000063514; ENSMUSG00000044702. [Q3U0P1-3]
ENSMUST00000098068; ENSMUSP00000095675; ENSMUSG00000044702. [Q3U0P1-1]
ENSMUST00000106469; ENSMUSP00000102077; ENSMUSG00000044702. [Q3U0P1-2]
ENSMUST00000130149; ENSMUSP00000121994; ENSMUSG00000044702. [Q3U0P1-4]
GeneID233826.
KEGGmmu:233826.
UCSCuc009joj.1. mouse. [Q3U0P1-1]
uc009jom.1. mouse. [Q3U0P1-4]
uc012ftg.1. mouse. [Q3U0P1-2]
uc012fth.1. mouse. [Q3U0P1-3]

Organism-specific databases

CTD79728.
MGIMGI:3040695. Palb2.

Phylogenomic databases

eggNOGNOG73403.
GeneTreeENSGT00390000014423.
HOGENOMHOG000115428.
HOVERGENHBG082102.
InParanoidQ3U0P1.
KOK10897.
OMANIVIWNL.
OrthoDBEOG72C51Z.
PhylomeDBQ3U0P1.
TreeFamTF351544.

Gene expression databases

ArrayExpressQ3U0P1.
BgeeQ3U0P1.
CleanExMM_PALB2.
GenevestigatorQ3U0P1.

Family and domain databases

Gene3D2.130.10.10. 3 hits.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
SUPFAMSSF50978. SSF50978. 3 hits.
ProtoNetSearch...

Other

ChiTaRSPALB2. mouse.
NextBio381867.
PROQ3U0P1.
SOURCESearch...

Entry information

Entry namePALB2_MOUSE
AccessionPrimary (citable) accession number: Q3U0P1
Secondary accession number(s): Q6NZG9, Q7TMQ4, Q8CEA9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot