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Protein

Prolyl 3-hydroxylase OGFOD1

Gene

Ogfod1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit RPS23, thereby regulating protein translation termination efficiency. Involved in stress granule formation (By similarity).By similarity

Catalytic activityi

Peptidyl L-proline + 2-oxoglutarate + O2 = peptidyl trans-3-hydroxy-L-proline + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:
  • Fe2+PROSITE-ProRule annotationNote: Binds 1 Fe2+ ion per subunit.PROSITE-ProRule annotation
  • L-ascorbateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi155IronPROSITE-ProRule annotation1
Metal bindingi157IronPROSITE-ProRule annotation1
Metal bindingi218IronPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase OGFOD1 (EC:1.14.11.-)
Alternative name(s):
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1
Gene namesi
Name:Ogfod1
Synonyms:Kiaa1612
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2442978. Ogfod1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002889751 – 545Prolyl 3-hydroxylase OGFOD1Add BLAST545

Proteomic databases

MaxQBiQ3U0K8.
PaxDbiQ3U0K8.
PeptideAtlasiQ3U0K8.
PRIDEiQ3U0K8.

PTM databases

iPTMnetiQ3U0K8.
PhosphoSitePlusiQ3U0K8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033009.
ExpressionAtlasiQ3U0K8. baseline and differential.
GenevisibleiQ3U0K8. MM.

Interactioni

Protein-protein interaction databases

BioGridi234757. 1 interactor.
STRINGi10090.ENSMUSP00000105183.

Structurei

3D structure databases

ProteinModelPortaliQ3U0K8.
SMRiQ3U0K8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini137 – 239Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST103

Sequence similaritiesi

Belongs to the TPA1 family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3844. Eukaryota.
COG3751. LUCA.
GeneTreeiENSGT00390000002349.
HOGENOMiHOG000007015.
HOVERGENiHBG056995.
InParanoidiQ3U0K8.
OMAiFSFVYYE.
OrthoDBiEOG091G08PF.
PhylomeDBiQ3U0K8.
TreeFamiTF105920.

Family and domain databases

InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR019601. Oxoglutarate/Fe-dep_Oase_C.
IPR006620. Pro_4_hyd_alph.
[Graphical view]
PfamiPF10637. Ofd1_CTDD. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3U0K8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNGKRPADPG PARPMKKGKK QVSAEFSDAV TEEILRKQVA EAWSCRTPFS
60 70 80 90 100
HEAIALDMDP FLHCVIPNFI QSQDFLEGLH KELLSLDFHE KYNDLYKFQQ
110 120 130 140 150
SDDLKNRKEP HISALRKLMF EDFRAWLSKV SGIDLEPTID MSCAKYEFTD
160 170 180 190 200
ALLCHDDELE GRRIAFILYL VPSWDRDLGG TLDLYDTDEH LQPKQIVKSL
210 220 230 240 250
IPSWNKLVFF EVSPVSFHQV SEVLSEETSR LSISGWFYGP SLTRPPTYFE
260 270 280 290 300
PPIPRNPHIP QDHEILYEWI NPAYLEMDYQ MQIQEEFEER SEILLKEFLK
310 320 330 340 350
PEKFAEVCEA LEKGDVEWKS HGPPNKRFYE KAEENNLPDV LKECMGLFRS
360 370 380 390 400
EALFLLLSNL TGLKLHFLAP SEDDETEEKG EGETASAAAG TEEGTSRRPS
410 420 430 440 450
GPENNQVAAG SHSQENGEQA DPEAQEEEAK KESSVPMCQG ELRRWKTGHY
460 470 480 490 500
TLVHDNTKTE FALDLFLYCG CEGWEPEYGG FTSYIAKGED EELLIVNPEN
510 520 530 540
NSLALVYRDR ETLRFVKHIN HRSLEQSKAF PSRSGFWDFA FIYYE
Length:545
Mass (Da):62,734
Last modified:October 11, 2005 - v1
Checksum:i92F376ABC86CB410
GO
Isoform 2 (identifier: Q3U0K8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     220-262: Missing.

Show »
Length:502
Mass (Da):57,854
Checksum:iB726F1A267873FC9
GO
Isoform 3 (identifier: Q3U0K8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     52-52: Missing.

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):62,605
Checksum:iCE6A47CC524D6948
GO

Sequence cautioni

The sequence AAH40267 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12A → V in BAE38717 (PubMed:16141072).Curated1
Sequence conflicti48P → S in BAE38717 (PubMed:16141072).Curated1
Sequence conflicti58M → V in BAE38717 (PubMed:16141072).Curated1
Sequence conflicti80H → Q in BAE38717 (PubMed:16141072).Curated1
Sequence conflicti397R → Q in BAE38717 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02585352Missing in isoform 3. 1 Publication1
Alternative sequenceiVSP_025854220 – 262Missing in isoform 2. 2 PublicationsAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK156765 mRNA. Translation: BAE33844.1.
AK164189 mRNA. Translation: BAE37671.1.
AK166343 mRNA. Translation: BAE38717.1.
BC040267 mRNA. Translation: AAH40267.1. Different initiation.
AK122532 mRNA. Translation: BAC65814.1.
CCDSiCCDS22535.2. [Q3U0K8-1]
CCDS52636.1. [Q3U0K8-2]
RefSeqiNP_001087226.1. NM_001093757.1. [Q3U0K8-2]
NP_808435.3. NM_177767.4. [Q3U0K8-1]
UniGeneiMm.251085.

Genome annotation databases

EnsembliENSMUST00000093301; ENSMUSP00000090991; ENSMUSG00000033009. [Q3U0K8-2]
ENSMUST00000109556; ENSMUSP00000105183; ENSMUSG00000033009. [Q3U0K8-1]
GeneIDi270086.
KEGGimmu:270086.
UCSCiuc009mvq.1. mouse. [Q3U0K8-1]
uc009mvr.1. mouse. [Q3U0K8-2]
uc012gim.1. mouse. [Q3U0K8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK156765 mRNA. Translation: BAE33844.1.
AK164189 mRNA. Translation: BAE37671.1.
AK166343 mRNA. Translation: BAE38717.1.
BC040267 mRNA. Translation: AAH40267.1. Different initiation.
AK122532 mRNA. Translation: BAC65814.1.
CCDSiCCDS22535.2. [Q3U0K8-1]
CCDS52636.1. [Q3U0K8-2]
RefSeqiNP_001087226.1. NM_001093757.1. [Q3U0K8-2]
NP_808435.3. NM_177767.4. [Q3U0K8-1]
UniGeneiMm.251085.

3D structure databases

ProteinModelPortaliQ3U0K8.
SMRiQ3U0K8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234757. 1 interactor.
STRINGi10090.ENSMUSP00000105183.

PTM databases

iPTMnetiQ3U0K8.
PhosphoSitePlusiQ3U0K8.

Proteomic databases

MaxQBiQ3U0K8.
PaxDbiQ3U0K8.
PeptideAtlasiQ3U0K8.
PRIDEiQ3U0K8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093301; ENSMUSP00000090991; ENSMUSG00000033009. [Q3U0K8-2]
ENSMUST00000109556; ENSMUSP00000105183; ENSMUSG00000033009. [Q3U0K8-1]
GeneIDi270086.
KEGGimmu:270086.
UCSCiuc009mvq.1. mouse. [Q3U0K8-1]
uc009mvr.1. mouse. [Q3U0K8-2]
uc012gim.1. mouse. [Q3U0K8-3]

Organism-specific databases

CTDi55239.
MGIiMGI:2442978. Ogfod1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3844. Eukaryota.
COG3751. LUCA.
GeneTreeiENSGT00390000002349.
HOGENOMiHOG000007015.
HOVERGENiHBG056995.
InParanoidiQ3U0K8.
OMAiFSFVYYE.
OrthoDBiEOG091G08PF.
PhylomeDBiQ3U0K8.
TreeFamiTF105920.

Miscellaneous databases

PROiQ3U0K8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033009.
ExpressionAtlasiQ3U0K8. baseline and differential.
GenevisibleiQ3U0K8. MM.

Family and domain databases

InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR019601. Oxoglutarate/Fe-dep_Oase_C.
IPR006620. Pro_4_hyd_alph.
[Graphical view]
PfamiPF10637. Ofd1_CTDD. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOGFD1_MOUSE
AccessioniPrimary (citable) accession number: Q3U0K8
Secondary accession number(s): Q3TLS5
, Q3TPR4, Q80TB3, Q8CFR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Contains Thr-228 instead of the expected predicted active site Lys.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.