Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q3U0K8 (OGFD1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prolyl 3-hydroxylase OGFOD1

EC=1.14.11.-
Alternative name(s):
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1
Gene names
Name:Ogfod1
Synonyms:Kiaa1612
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length545 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit RPS23, thereby regulating protein translation termination efficiency. Involved in stress granule formation By similarity.

Catalytic activity

Peptidyl L-proline + 2-oxoglutarate + O2 = peptidyl trans-3-hydroxy-L-proline + succinate + CO2.

Cofactor

Binds 1 Fe2+ ion per subunit By similarity.

Ascorbate By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the TPA1 family.

Contains 1 Fe2OG dioxygenase domain.

Caution

Contains Thr-228 instead of the expected predicted active site Lys.

Sequence caution

The sequence AAH40267.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3U0K8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3U0K8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     220-262: Missing.
Isoform 3 (identifier: Q3U0K8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     52-52: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 545545Prolyl 3-hydroxylase OGFOD1
PRO_0000288975

Regions

Domain137 – 239103Fe2OG dioxygenase

Sites

Metal binding1551Iron By similarity
Metal binding1571Iron By similarity
Metal binding2181Iron By similarity

Natural variations

Alternative sequence521Missing in isoform 3.
VSP_025853
Alternative sequence220 – 26243Missing in isoform 2.
VSP_025854

Experimental info

Sequence conflict121A → V in BAE38717. Ref.1
Sequence conflict481P → S in BAE38717. Ref.1
Sequence conflict581M → V in BAE38717. Ref.1
Sequence conflict801H → Q in BAE38717. Ref.1
Sequence conflict3971R → Q in BAE38717. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 92F376ABC86CB410

FASTA54562,734
        10         20         30         40         50         60 
MNGKRPADPG PARPMKKGKK QVSAEFSDAV TEEILRKQVA EAWSCRTPFS HEAIALDMDP 

        70         80         90        100        110        120 
FLHCVIPNFI QSQDFLEGLH KELLSLDFHE KYNDLYKFQQ SDDLKNRKEP HISALRKLMF 

       130        140        150        160        170        180 
EDFRAWLSKV SGIDLEPTID MSCAKYEFTD ALLCHDDELE GRRIAFILYL VPSWDRDLGG 

       190        200        210        220        230        240 
TLDLYDTDEH LQPKQIVKSL IPSWNKLVFF EVSPVSFHQV SEVLSEETSR LSISGWFYGP 

       250        260        270        280        290        300 
SLTRPPTYFE PPIPRNPHIP QDHEILYEWI NPAYLEMDYQ MQIQEEFEER SEILLKEFLK 

       310        320        330        340        350        360 
PEKFAEVCEA LEKGDVEWKS HGPPNKRFYE KAEENNLPDV LKECMGLFRS EALFLLLSNL 

       370        380        390        400        410        420 
TGLKLHFLAP SEDDETEEKG EGETASAAAG TEEGTSRRPS GPENNQVAAG SHSQENGEQA 

       430        440        450        460        470        480 
DPEAQEEEAK KESSVPMCQG ELRRWKTGHY TLVHDNTKTE FALDLFLYCG CEGWEPEYGG 

       490        500        510        520        530        540 
FTSYIAKGED EELLIVNPEN NSLALVYRDR ETLRFVKHIN HRSLEQSKAF PSRSGFWDFA 


FIYYE 

« Hide

Isoform 2 [UniParc].

Checksum: B726F1A267873FC9
Show »

FASTA50257,854
Isoform 3 [UniParc].

Checksum: CE6A47CC524D6948
Show »

FASTA54462,605

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-545 (ISOFORM 2).
Strain: C57BL/6J and NOD.
Tissue: Hippocampus, Mammary gland and Spleen.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Eye.
[3]"Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-545 (ISOFORM 3).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK156765 mRNA. Translation: BAE33844.1.
AK164189 mRNA. Translation: BAE37671.1.
AK166343 mRNA. Translation: BAE38717.1.
BC040267 mRNA. Translation: AAH40267.1. Different initiation.
AK122532 mRNA. Translation: BAC65814.1.
CCDSCCDS22535.2. [Q3U0K8-1]
CCDS52636.1. [Q3U0K8-2]
RefSeqNP_001087226.1. NM_001093757.1. [Q3U0K8-2]
NP_808435.3. NM_177767.4. [Q3U0K8-1]
UniGeneMm.251085.

3D structure databases

ProteinModelPortalQ3U0K8.
SMRQ3U0K8. Positions 72-329, 441-519.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid234757. 1 interaction.
STRING10090.ENSMUSP00000105183.

PTM databases

PhosphoSiteQ3U0K8.

Proteomic databases

MaxQBQ3U0K8.
PaxDbQ3U0K8.
PRIDEQ3U0K8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000093301; ENSMUSP00000090991; ENSMUSG00000033009. [Q3U0K8-2]
ENSMUST00000109556; ENSMUSP00000105183; ENSMUSG00000033009. [Q3U0K8-1]
GeneID270086.
KEGGmmu:270086.
UCSCuc009mvq.1. mouse. [Q3U0K8-1]
uc009mvr.1. mouse. [Q3U0K8-2]
uc012gim.1. mouse. [Q3U0K8-3]

Organism-specific databases

CTD55239.
MGIMGI:2442978. Ogfod1.
RougeSearch...

Phylogenomic databases

eggNOGCOG3751.
GeneTreeENSGT00390000002349.
HOGENOMHOG000007015.
HOVERGENHBG056995.
InParanoidQ3U0K8.
OMAFSFVYYE.
OrthoDBEOG7FBRHW.
PhylomeDBQ3U0K8.
TreeFamTF105920.

Gene expression databases

ArrayExpressQ3U0K8.
BgeeQ3U0K8.
GenevestigatorQ3U0K8.

Family and domain databases

InterProIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR019601. Oxoglutarate/Fe-dep_Oase_C.
IPR006620. Pro_4_hyd_alph.
[Graphical view]
PfamPF13640. 2OG-FeII_Oxy_3. 1 hit.
PF10637. Ofd1_CTDD. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio393192.
PROQ3U0K8.
SOURCESearch...

Entry information

Entry nameOGFD1_MOUSE
AccessionPrimary (citable) accession number: Q3U0K8
Secondary accession number(s): Q3TLS5 expand/collapse secondary AC list , Q3TPR4, Q80TB3, Q8CFR6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot