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Protein

E3 ubiquitin-protein ligase HACE1

Gene

Hace1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (By similarity). May also act as a transcription regulator via its interaction with RARB.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei876Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HACE1 (EC:2.3.2.26)
Alternative name(s):
HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1
HECT-type E3 ubiquitin transferase HACE1
Gene namesi
Name:Hace1
Synonyms:Kiaa1320
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2446110. Hace1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice develop spontaneous, late-onset cancer. Moreover, tumor incidence in mice heterozygous for a p53/Tp53 mutation in higher in a Hace1-deficient background.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002806231 – 909E3 ubiquitin-protein ligase HACE1Add BLAST909

Proteomic databases

MaxQBiQ3U0D9.
PaxDbiQ3U0D9.
PeptideAtlasiQ3U0D9.
PRIDEiQ3U0D9.

PTM databases

iPTMnetiQ3U0D9.
PhosphoSitePlusiQ3U0D9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038822.
ExpressionAtlasiQ3U0D9. baseline and differential.
GenevisibleiQ3U0D9. MM.

Interactioni

Subunit structurei

Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit (By similarity). Interacts with RARB.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229081. 1 interactor.
IntActiQ3U0D9. 1 interactor.
STRINGi10090.ENSMUSP00000039206.

Structurei

3D structure databases

ProteinModelPortaliQ3U0D9.
SMRiQ3U0D9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati64 – 93ANK 1Add BLAST30
Repeati97 – 126ANK 2Add BLAST30
Repeati130 – 159ANK 3Add BLAST30
Repeati163 – 192ANK 4Add BLAST30
Repeati196 – 226ANK 5Add BLAST31
Repeati228 – 257ANK 6Add BLAST30
Domaini574 – 909HECTPROSITE-ProRule annotationAdd BLAST336

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0939. Eukaryota.
KOG4177. Eukaryota.
COG0666. LUCA.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
HOGENOMiHOG000208454.
HOVERGENiHBG004134.
InParanoidiQ3U0D9.
KOiK12166.
OMAiTCEILIQ.
OrthoDBiEOG091G025R.
PhylomeDBiQ3U0D9.
TreeFamiTF323417.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000569. HECT_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00632. HECT. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
SM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50237. HECT. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3U0D9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL
60 70 80 90 100
SNSKFDVNYA FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT
110 120 130 140 150
PLHLAARNGQ KKCMSKLLEY SADVNICNNE GLTAIHWLAV NGRTELLHDL
160 170 180 190 200
VQHVTDVDVE DAMGQTALHV ACQNGHKTTV QCLLDSGADI NRPNVSGATP
210 220 230 240 250
LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG YGQTCEVLIQ
260 270 280 290 300
YHPRLFQTIV QMTQNEDLRE NMLRQVLQHL SQQSESQYLK ILTGLAEVAT
310 320 330 340 350
TNGHKLLSLS SNYDAQMKSL LRIVRIFCHV FRIGPSSPSN GIDMGYNGNK
360 370 380 390 400
TPRSQVFKPL ELLWHSLDEW LVLIATELMK NKEDSTDITS ILLKQKGQDQ
410 420 430 440 450
EAPSLSAFEP PGPGSYESLP PGPGDSKPEV LAGEQEASAD CQDVISVTAN
460 470 480 490 500
RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV CKHDEVLKCF VNRNPKIIFD
510 520 530 540 550
HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP DSDMVHRPVS
560 570 580 590 600
ENDILLVHRD SIFRSSCEIV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW
610 620 630 640 650
FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL
660 670 680 690 700
ALNHRQLVNI YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS
710 720 730 740 750
DLGLELTFSV ETDVFGAMEE VPLKPGGGSI LVTQNNKAEY VQLVTELRMT
760 770 780 790 800
RAIQPQINAF LQGFHMFIPP SLIQLFDEYE LELLLSGMPE IDVNDWIKNT
810 820 830 840 850
EYTSGYERED PVIQWFWEVV EDITQEERVL LLQFVTGSSR VPHGGFANIM
860 870 880 890 900
GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH

CGSYGYTMA
Length:909
Mass (Da):102,114
Last modified:October 11, 2005 - v1
Checksum:i804F62A06E9C78A0
GO
Isoform 2 (identifier: Q3U0D9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     838-838: S → R
     839-909: Missing.

Note: No experimental confirmation available.
Show »
Length:838
Mass (Da):94,573
Checksum:iB51C2820FDA66E0F
GO
Isoform 3 (identifier: Q3U0D9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     738-781: Missing.

Note: No experimental confirmation available.
Show »
Length:865
Mass (Da):96,919
Checksum:i684FEBC9763DC622
GO
Isoform 4 (identifier: Q3U0D9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     738-781: Missing.
     814-832: Missing.

Note: No experimental confirmation available.
Show »
Length:846
Mass (Da):94,505
Checksum:i8367FBDA8EA0829F
GO

Sequence cautioni

The sequence AAH25474 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92N → S in BAC31390 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023832738 – 781Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST44
Alternative sequenceiVSP_042379814 – 832Missing in isoform 4. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_023833838S → R in isoform 2. 1 Publication1
Alternative sequenceiVSP_023834839 – 909Missing in isoform 2. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042879 mRNA. Translation: BAC31390.1.
AK156958 mRNA. Translation: BAE33915.1.
AC135669 Genomic DNA. No translation available.
AC153847 Genomic DNA. No translation available.
BC025227 mRNA. Translation: AAH25227.1.
BC025474 mRNA. Translation: AAH25474.1. Different initiation.
BC120695 mRNA. Translation: AAI20696.1.
BC120697 mRNA. Translation: AAI20698.1.
AK220383 mRNA. Translation: BAD90440.1.
CCDSiCCDS23829.1. [Q3U0D9-1]
RefSeqiNP_766061.2. NM_172473.3. [Q3U0D9-1]
UniGeneiMm.458633.

Genome annotation databases

EnsembliENSMUST00000037044; ENSMUSP00000039206; ENSMUSG00000038822. [Q3U0D9-1]
GeneIDi209462.
KEGGimmu:209462.
UCSCiuc007fad.2. mouse. [Q3U0D9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042879 mRNA. Translation: BAC31390.1.
AK156958 mRNA. Translation: BAE33915.1.
AC135669 Genomic DNA. No translation available.
AC153847 Genomic DNA. No translation available.
BC025227 mRNA. Translation: AAH25227.1.
BC025474 mRNA. Translation: AAH25474.1. Different initiation.
BC120695 mRNA. Translation: AAI20696.1.
BC120697 mRNA. Translation: AAI20698.1.
AK220383 mRNA. Translation: BAD90440.1.
CCDSiCCDS23829.1. [Q3U0D9-1]
RefSeqiNP_766061.2. NM_172473.3. [Q3U0D9-1]
UniGeneiMm.458633.

3D structure databases

ProteinModelPortaliQ3U0D9.
SMRiQ3U0D9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229081. 1 interactor.
IntActiQ3U0D9. 1 interactor.
STRINGi10090.ENSMUSP00000039206.

PTM databases

iPTMnetiQ3U0D9.
PhosphoSitePlusiQ3U0D9.

Proteomic databases

MaxQBiQ3U0D9.
PaxDbiQ3U0D9.
PeptideAtlasiQ3U0D9.
PRIDEiQ3U0D9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037044; ENSMUSP00000039206; ENSMUSG00000038822. [Q3U0D9-1]
GeneIDi209462.
KEGGimmu:209462.
UCSCiuc007fad.2. mouse. [Q3U0D9-1]

Organism-specific databases

CTDi57531.
MGIiMGI:2446110. Hace1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0939. Eukaryota.
KOG4177. Eukaryota.
COG0666. LUCA.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
HOGENOMiHOG000208454.
HOVERGENiHBG004134.
InParanoidiQ3U0D9.
KOiK12166.
OMAiTCEILIQ.
OrthoDBiEOG091G025R.
PhylomeDBiQ3U0D9.
TreeFamiTF323417.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ3U0D9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038822.
ExpressionAtlasiQ3U0D9. baseline and differential.
GenevisibleiQ3U0D9. MM.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000569. HECT_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00632. HECT. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
SM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50237. HECT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHACE1_MOUSE
AccessioniPrimary (citable) accession number: Q3U0D9
Secondary accession number(s): F6VQI5
, F7ALT5, Q5DTY7, Q8BXY2, Q8R160, Q8R3G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.