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Protein

E3 ubiquitin-protein ligase HACE1

Gene

Hace1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (By similarity). May also act as a transcription regulator via its interaction with RARB.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei876Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HACE1 (EC:2.3.2.26)
Alternative name(s):
HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1
HECT-type E3 ubiquitin transferase HACE1
Gene namesi
Name:Hace1
Synonyms:Kiaa1320
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2446110 Hace1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice develop spontaneous, late-onset cancer. Moreover, tumor incidence in mice heterozygous for a p53/Tp53 mutation in higher in a Hace1-deficient background.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002806231 – 909E3 ubiquitin-protein ligase HACE1Add BLAST909

Proteomic databases

MaxQBiQ3U0D9
PaxDbiQ3U0D9
PeptideAtlasiQ3U0D9
PRIDEiQ3U0D9

PTM databases

iPTMnetiQ3U0D9
PhosphoSitePlusiQ3U0D9

Expressioni

Gene expression databases

BgeeiENSMUSG00000038822
ExpressionAtlasiQ3U0D9 baseline and differential
GenevisibleiQ3U0D9 MM

Interactioni

Subunit structurei

Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit (By similarity). Interacts with RARB.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229081, 1 interactor
IntActiQ3U0D9, 1 interactor
STRINGi10090.ENSMUSP00000039206

Structurei

3D structure databases

ProteinModelPortaliQ3U0D9
SMRiQ3U0D9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati64 – 93ANK 1Add BLAST30
Repeati97 – 126ANK 2Add BLAST30
Repeati130 – 159ANK 3Add BLAST30
Repeati163 – 192ANK 4Add BLAST30
Repeati196 – 226ANK 5Add BLAST31
Repeati228 – 257ANK 6Add BLAST30
Domaini574 – 909HECTPROSITE-ProRule annotationAdd BLAST336

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0939 Eukaryota
KOG4177 Eukaryota
COG0666 LUCA
COG5021 LUCA
GeneTreeiENSGT00760000118966
HOGENOMiHOG000208454
HOVERGENiHBG004134
InParanoidiQ3U0D9
KOiK12166
OMAiNDMGYNG
OrthoDBiEOG091G025R
PhylomeDBiQ3U0D9
TreeFamiTF323417

Family and domain databases

CDDicd00204 ANK, 1 hit
cd00078 HECTc, 1 hit
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00632 HECT, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SM00119 HECTc, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 5 hits
PS50237 HECT, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3U0D9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL
60 70 80 90 100
SNSKFDVNYA FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT
110 120 130 140 150
PLHLAARNGQ KKCMSKLLEY SADVNICNNE GLTAIHWLAV NGRTELLHDL
160 170 180 190 200
VQHVTDVDVE DAMGQTALHV ACQNGHKTTV QCLLDSGADI NRPNVSGATP
210 220 230 240 250
LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG YGQTCEVLIQ
260 270 280 290 300
YHPRLFQTIV QMTQNEDLRE NMLRQVLQHL SQQSESQYLK ILTGLAEVAT
310 320 330 340 350
TNGHKLLSLS SNYDAQMKSL LRIVRIFCHV FRIGPSSPSN GIDMGYNGNK
360 370 380 390 400
TPRSQVFKPL ELLWHSLDEW LVLIATELMK NKEDSTDITS ILLKQKGQDQ
410 420 430 440 450
EAPSLSAFEP PGPGSYESLP PGPGDSKPEV LAGEQEASAD CQDVISVTAN
460 470 480 490 500
RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV CKHDEVLKCF VNRNPKIIFD
510 520 530 540 550
HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP DSDMVHRPVS
560 570 580 590 600
ENDILLVHRD SIFRSSCEIV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW
610 620 630 640 650
FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL
660 670 680 690 700
ALNHRQLVNI YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS
710 720 730 740 750
DLGLELTFSV ETDVFGAMEE VPLKPGGGSI LVTQNNKAEY VQLVTELRMT
760 770 780 790 800
RAIQPQINAF LQGFHMFIPP SLIQLFDEYE LELLLSGMPE IDVNDWIKNT
810 820 830 840 850
EYTSGYERED PVIQWFWEVV EDITQEERVL LLQFVTGSSR VPHGGFANIM
860 870 880 890 900
GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH

CGSYGYTMA
Length:909
Mass (Da):102,114
Last modified:October 11, 2005 - v1
Checksum:i804F62A06E9C78A0
GO
Isoform 2 (identifier: Q3U0D9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     838-838: S → R
     839-909: Missing.

Note: No experimental confirmation available.
Show »
Length:838
Mass (Da):94,573
Checksum:iB51C2820FDA66E0F
GO
Isoform 3 (identifier: Q3U0D9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     738-781: Missing.

Note: No experimental confirmation available.
Show »
Length:865
Mass (Da):96,919
Checksum:i684FEBC9763DC622
GO
Isoform 4 (identifier: Q3U0D9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     738-781: Missing.
     814-832: Missing.

Note: No experimental confirmation available.
Show »
Length:846
Mass (Da):94,505
Checksum:i8367FBDA8EA0829F
GO

Sequence cautioni

The sequence AAH25474 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92N → S in BAC31390 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023832738 – 781Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST44
Alternative sequenceiVSP_042379814 – 832Missing in isoform 4. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_023833838S → R in isoform 2. 1 Publication1
Alternative sequenceiVSP_023834839 – 909Missing in isoform 2. 1 PublicationAdd BLAST71

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042879 mRNA Translation: BAC31390.1
AK156958 mRNA Translation: BAE33915.1
AC135669 Genomic DNA No translation available.
AC153847 Genomic DNA No translation available.
BC025227 mRNA Translation: AAH25227.1
BC025474 mRNA Translation: AAH25474.1 Different initiation.
BC120695 mRNA Translation: AAI20696.1
BC120697 mRNA Translation: AAI20698.1
AK220383 mRNA Translation: BAD90440.1
CCDSiCCDS23829.1 [Q3U0D9-1]
RefSeqiNP_766061.2, NM_172473.3 [Q3U0D9-1]
UniGeneiMm.458633

Genome annotation databases

EnsembliENSMUST00000037044; ENSMUSP00000039206; ENSMUSG00000038822 [Q3U0D9-1]
GeneIDi209462
KEGGimmu:209462
UCSCiuc007fad.2 mouse [Q3U0D9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHACE1_MOUSE
AccessioniPrimary (citable) accession number: Q3U0D9
Secondary accession number(s): F6VQI5
, F7ALT5, Q5DTY7, Q8BXY2, Q8R160, Q8R3G4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 11, 2005
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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