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Protein

Extended synaptotagmin-2

Gene

Esyt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tethers the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane. Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport. Plays a role in FGF signaling via its role in the rapid internalization of FGFR1 that has been activated by FGF1 binding; this occurs most likely via the AP-2 complex (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi324Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi325Calcium 1By similarity1
Metal bindingi325Calcium 2By similarity1
Metal bindingi337Calcium 2By similarity1
Metal bindingi384Calcium 1By similarity1
Metal bindingi384Calcium 2By similarity1
Metal bindingi385Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi386Calcium 1By similarity1
Metal bindingi386Calcium 2By similarity1
Metal bindingi386Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi388Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi390Calcium 3By similarity1
Metal bindingi391Calcium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis, Lipid transport, Transport

Keywords - Ligandi

Calcium, Lipid-binding, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Extended synaptotagmin-2
Short name:
E-Syt2
Gene namesi
Name:Esyt2
Synonyms:D12Ertd551e, Fam62b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1261845. Esyt2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 27CytoplasmicSequence analysisAdd BLAST27
Transmembranei28 – 48HelicalSequence analysisAdd BLAST21
Topological domaini49 – 51LumenalSequence analysis3
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Topological domaini73 – 845CytoplasmicSequence analysisAdd BLAST773

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782591 – 845Extended synaptotagmin-2Add BLAST845

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei615PhosphoserineBy similarity1
Modified residuei617PhosphoserineBy similarity1
Modified residuei629PhosphothreonineBy similarity1
Modified residuei660PhosphoserineCombined sources1
Modified residuei662PhosphoserineCombined sources1
Modified residuei663PhosphoserineCombined sources1
Modified residuei667PhosphoserineCombined sources1
Modified residuei679PhosphoserineCombined sources1
Modified residuei682PhosphoserineCombined sources1
Modified residuei685PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3TZZ7.
MaxQBiQ3TZZ7.
PaxDbiQ3TZZ7.
PeptideAtlasiQ3TZZ7.
PRIDEiQ3TZZ7.

PTM databases

iPTMnetiQ3TZZ7.
PhosphoSitePlusiQ3TZZ7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021171.
CleanExiMM_D12ERTD551E.
GenevisibleiQ3TZZ7. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with ESYT1 and ESYT3. Interacts with FGFR1 that has been activated by FGF1 binding. Interacts with the AP-2 complex; identified in a complex with the AP-2 complex and FGFR1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ3TZZ7. 1 interactor.
MINTiMINT-4392453.
STRINGi10090.ENSMUSP00000098548.

Structurei

3D structure databases

ProteinModelPortaliQ3TZZ7.
SMRiQ3TZZ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini296 – 397C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini447 – 541C2 2PROSITE-ProRule annotationAdd BLAST95
Domaini712 – 816C2 3PROSITE-ProRule annotationAdd BLAST105

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 294Glycerophospholipid-binding barrel-like domainBy similarityAdd BLAST176
Regioni757 – 764Required for phosphatidylinositol 4,5-bisphosphate-dependent location at the cell membraneBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi660 – 663Poly-Ser4

Domaini

Anchored to the endoplasmic reticulum membrane by a transmembrane hairpin structure; both N-terminus and C-terminus are cytoplasmic.By similarity
The C2 domains mediate lipid and calcium binding. The N-terminal C2 domain binds calcium ions and is important for calcium-dependent lipid binding and interaction with membranes. Two calcium ions are bound at a high-affinity site and a third calcium ion is bound with lower affinity. May bind up to four calcium ions. In contrast, the second C2 domain apparently does not bind calcium. The third C2 domain mediates interaction with membranes enriched in phosphatidylinositol 4,5-bisphosphate and is required for location at the cell membrane (By similarity).By similarity
Contains a barrel-like domain that binds glycerophospholipids in its interior; can bind two lipid molecules simultaneously. Binds a variety of lipids, including phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol (By similarity).By similarity

Sequence similaritiesi

Belongs to the extended synaptotagmin family.Curated
Contains 3 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410ISCC. Eukaryota.
ENOG410XPR4. LUCA.
GeneTreeiENSGT00550000074417.
HOGENOMiHOG000043080.
HOVERGENiHBG055795.
InParanoidiQ3TZZ7.
OMAiFIEMGQF.
OrthoDBiEOG091G02X7.
PhylomeDBiQ3TZZ7.
TreeFamiTF324255.

Family and domain databases

Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR031468. SMP_LBD.
[Graphical view]
PfamiPF00168. C2. 3 hits.
PF17047. SMP_LBD. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 3 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TZZ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSAGGEGPE AGPGRAGGRS EPEAPGSALS VDLPGLLGQL ARSFALLLPV
60 70 80 90 100
YALGYLGLSF SWVLLALGLL AWCRRSRGLK ASRLCRALAL LEDEEQAVRL
110 120 130 140 150
GVRACDLPAW VHFPDTERAE WLNKTVKHMW PFICQFIEKL FRETIEPAVR
160 170 180 190 200
GANAHLSTFS FTKVDVGQQP LRVNGVKVYT ENVDKRQIIL DLQISFVGNC
210 220 230 240 250
EIDLEIKRYF CRAGVKSIQI HGTMRVILEP LIGDMPLVGA LSIFFLRKPL
260 270 280 290 300
LEINWTGLTN LLDIPGLNGL SDTIILDIIS NYLVLPNRIT VPLVSEVQIA
310 320 330 340 350
QLRFPIPKGV LRIHFIEAQD LQGKDTYLKG LVKGKSDPYG IIRVGNQIFQ
360 370 380 390 400
SKVIKENLSP KWNEVYEALV YEHPGQELEI ELFDEDPDKD DFLGSLMIDL
410 420 430 440 450
IEVEKERLLD EWFTLDEVPK GKLHLKLEWL TLMPDAANLD KVLADIRADK
460 470 480 490 500
DQASDGLSSA LLILYLDSAR NLPSGKKINS NPNPLVQMSV GHKAQESKIR
510 520 530 540 550
YKTSEPVWEE NFTFFIHNPR RQDLEVEVKD EQHQCSLGSL RIPLSQLLTS
560 570 580 590 600
DNMTINQRFQ LSNSGPNSTL KMKIALRVLH LEKQERPPDY QHSAQVKRPS
610 620 630 640 650
VSKEGRKMPI KSQMSASPGT GGANTAPSTP VMGVDDKPAM EEKPQPPEAS
660 670 680 690 700
PLGHRDLGRS SSSLLASPSH IAAKEPTPSI ASDISLPIAT QELRQRLRQL
710 720 730 740 750
ENGTTLGQSP LGQIQLTIRH SSQRNKLIVV VHSCRNLIAF SEDGSDPYVR
760 770 780 790 800
MYLLPDKRRS GRRKTHVSKK TLNPVFDQSF DFSVSLPEVQ RRTLDVAVKN
810 820 830 840
SGGFLSKDKG LLGKVLVVLA SEELAKGWTQ WYDLTEDGTR PQVIT
Length:845
Mass (Da):94,139
Last modified:October 11, 2005 - v1
Checksum:i988F3D97D7F3CF6C
GO
Isoform 2 (identifier: Q3TZZ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-487: Missing.

Show »
Length:358
Mass (Da):39,884
Checksum:i6AE8B94A52027E24
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0232431 – 487Missing in isoform 2. 2 PublicationsAdd BLAST487

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014813 mRNA. Translation: BAB29565.1.
AK044360 mRNA. Translation: BAC31884.1.
AK157352 mRNA. Translation: BAE34059.1.
BC052440 mRNA. Translation: AAH52440.1.
BC054797 mRNA. Translation: AAH54797.1.
BC059230 mRNA. Translation: AAH59230.1.
BC138937 mRNA. Translation: AAI38938.1.
CCDSiCCDS26211.1. [Q3TZZ7-1]
RefSeqiNP_083007.2. NM_028731.5. [Q3TZZ7-1]
XP_017170617.1. XM_017315128.1. [Q3TZZ7-1]
UniGeneiMm.273755.

Genome annotation databases

EnsembliENSMUST00000100986; ENSMUSP00000098548; ENSMUSG00000021171. [Q3TZZ7-1]
GeneIDi52635.
KEGGimmu:52635.
UCSCiuc007pho.1. mouse. [Q3TZZ7-1]
uc007phq.1. mouse. [Q3TZZ7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014813 mRNA. Translation: BAB29565.1.
AK044360 mRNA. Translation: BAC31884.1.
AK157352 mRNA. Translation: BAE34059.1.
BC052440 mRNA. Translation: AAH52440.1.
BC054797 mRNA. Translation: AAH54797.1.
BC059230 mRNA. Translation: AAH59230.1.
BC138937 mRNA. Translation: AAI38938.1.
CCDSiCCDS26211.1. [Q3TZZ7-1]
RefSeqiNP_083007.2. NM_028731.5. [Q3TZZ7-1]
XP_017170617.1. XM_017315128.1. [Q3TZZ7-1]
UniGeneiMm.273755.

3D structure databases

ProteinModelPortaliQ3TZZ7.
SMRiQ3TZZ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3TZZ7. 1 interactor.
MINTiMINT-4392453.
STRINGi10090.ENSMUSP00000098548.

PTM databases

iPTMnetiQ3TZZ7.
PhosphoSitePlusiQ3TZZ7.

Proteomic databases

EPDiQ3TZZ7.
MaxQBiQ3TZZ7.
PaxDbiQ3TZZ7.
PeptideAtlasiQ3TZZ7.
PRIDEiQ3TZZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100986; ENSMUSP00000098548; ENSMUSG00000021171. [Q3TZZ7-1]
GeneIDi52635.
KEGGimmu:52635.
UCSCiuc007pho.1. mouse. [Q3TZZ7-1]
uc007phq.1. mouse. [Q3TZZ7-2]

Organism-specific databases

CTDi57488.
MGIiMGI:1261845. Esyt2.

Phylogenomic databases

eggNOGiENOG410ISCC. Eukaryota.
ENOG410XPR4. LUCA.
GeneTreeiENSGT00550000074417.
HOGENOMiHOG000043080.
HOVERGENiHBG055795.
InParanoidiQ3TZZ7.
OMAiFIEMGQF.
OrthoDBiEOG091G02X7.
PhylomeDBiQ3TZZ7.
TreeFamiTF324255.

Enzyme and pathway databases

ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.

Miscellaneous databases

PROiQ3TZZ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021171.
CleanExiMM_D12ERTD551E.
GenevisibleiQ3TZZ7. MM.

Family and domain databases

Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR031468. SMP_LBD.
[Graphical view]
PfamiPF00168. C2. 3 hits.
PF17047. SMP_LBD. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 3 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESYT2_MOUSE
AccessioniPrimary (citable) accession number: Q3TZZ7
Secondary accession number(s): B2RSN5, Q9D5Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.