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Protein

Apoptosis-inducing factor 3

Gene

Aifm3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Induces apoptosis through a caspase dependent pathway. Reduces mitochondrial membrane potential (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi109 – 1091Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi111 – 1111Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi128 – 1281Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi131 – 1311Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Binding sitei235 – 2351FADSequence analysis
Binding sitei240 – 2401FADSequence analysis
Binding sitei270 – 2701FAD; via amide nitrogen and carbonyl oxygenSequence analysis
Binding sitei467 – 4671FADSequence analysis
Binding sitei514 – 5141FAD; via carbonyl oxygenSequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi201 – 2055FADSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Apoptosis, Electron transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis-inducing factor 3 (EC:1.-.-.-)
Alternative name(s):
Apoptosis-inducing factor-like protein
Gene namesi
Name:Aifm3
Synonyms:Aifl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1919418. Aifm3.

Subcellular locationi

  • Mitochondrion

  • Note: Does not translocate to the nucleus upon induction of apoptosis.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 605605Apoptosis-inducing factor 3PRO_0000255661Add
BLAST

Proteomic databases

MaxQBiQ3TY86.
PaxDbiQ3TY86.
PRIDEiQ3TY86.

PTM databases

iPTMnetiQ3TY86.
PhosphoSiteiQ3TY86.

Expressioni

Gene expression databases

BgeeiQ3TY86.
GenevisibleiQ3TY86. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023448.

Structurei

3D structure databases

ProteinModelPortaliQ3TY86.
SMRiQ3TY86. Positions 72-170, 192-562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 16596RieskePROSITE-ProRule annotationAdd
BLAST

Domaini

The Rieske domain induces apoptosis.By similarity

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1336. Eukaryota.
COG0446. LUCA.
COG2146. LUCA.
GeneTreeiENSGT00530000063416.
HOGENOMiHOG000276711.
HOVERGENiHBG052926.
InParanoidiQ3TY86.
OMAiLHSKTGD.
OrthoDBiEOG77DJ5F.
PhylomeDBiQ3TY86.
TreeFamiTF314028.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
IPR028202. Reductase_C.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF14759. Reductase_C. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00469. PNDRDTASEII.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TY86-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGCFSKPKP VELKIEVVLP EKERGKEELS ASGKGSPRGY QGNGTARHFH
60 70 80 90 100
AEERLPTPQP YPSPQDCVEA TVCHVKDLEN GQMREVELGW GKVLLVKDNG
110 120 130 140 150
EFHALGHKCP HYGAPLVKGV LSRGRVRCPW HGACFNISTG DLEDFPGLDS
160 170 180 190 200
LHKFQVKIEK EKVTIRASKQ ALQLQRRTKV MAKCISPSAG HSSSTNVLIV
210 220 230 240 250
GAGAAGLVCA ETLRQEGFSD RIVLCTLDRH LPYDRAKLSK SLDAQPEQLA
260 270 280 290 300
LRPKEFFRAY GIEMLTEAQV VTVDVRNKKV VFKDGFKLEY SKLLLAPGSS
310 320 330 340 350
PKTLTCKGKD VENVFTIRTP EDANRVLRLA RGRNAVVVGA GFLGMEVAAY
360 370 380 390 400
LTEKAHSVSV VELEETPFRR FLGERVGRAL MKMFENNRVK FYMQTEVSEL
410 420 430 440 450
RAQEGKLQEV VLKSSKVLRA DVCVLGIGAV PATGFLRQSG IGLDSRGFIP
460 470 480 490 500
VNKMMQTNVP GVFAAGDAVT FPLAWRNNRK VNIPHWQMAH AQGRVAAQNM
510 520 530 540 550
LAQEAEINTV PYLWTAMFGK SLRYAGYGEG FDDVIIQGDL EELKFVAFYT
560 570 580 590 600
KSDEVIAVAS MNYDPIVSKV AEVLASGRAI RKREVELFML HSKTGDMSWL

TGKGS
Length:605
Mass (Da):66,792
Last modified:October 11, 2005 - v1
Checksum:iD4811CB2B2CD0739
GO
Isoform 2 (identifier: Q3TY86-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     587-593: Missing.

Note: No experimental confirmation available.
Show »
Length:598
Mass (Da):65,935
Checksum:iAF9A044EC3E59579
GO
Isoform 3 (identifier: Q3TY86-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     494-500: RVAAQNM → MASPEAS
     501-605: Missing.

Show »
Length:500
Mass (Da):55,040
Checksum:i2A456EA3D4E8B4EF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti138 – 1381S → T in BAC33988 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei494 – 5007RVAAQNM → MASPEAS in isoform 3. 2 PublicationsVSP_021304
Alternative sequencei501 – 605105Missing in isoform 3. 2 PublicationsVSP_021305Add
BLAST
Alternative sequencei587 – 5937Missing in isoform 2. 1 PublicationVSP_021306

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049928 mRNA. Translation: BAC33988.1.
AK158809 mRNA. Translation: BAE34677.1.
BC096476 mRNA. Translation: AAH96476.1.
BC120685 mRNA. Translation: AAI20686.1.
CCDSiCCDS28003.1. [Q3TY86-2]
CCDS79430.1. [Q3TY86-1]
RefSeqiNP_001277999.1. NM_001291070.1. [Q3TY86-1]
NP_780387.2. NM_175178.4. [Q3TY86-2]
XP_006522655.1. XM_006522592.2. [Q3TY86-1]
UniGeneiMm.40038.

Genome annotation databases

EnsembliENSMUST00000023448; ENSMUSP00000023448; ENSMUSG00000022763. [Q3TY86-2]
ENSMUST00000115685; ENSMUSP00000111349; ENSMUSG00000022763. [Q3TY86-1]
GeneIDi72168.
KEGGimmu:72168.
UCSCiuc007ykw.2. mouse. [Q3TY86-1]
uc007ykx.2. mouse. [Q3TY86-3]
uc007yky.2. mouse. [Q3TY86-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049928 mRNA. Translation: BAC33988.1.
AK158809 mRNA. Translation: BAE34677.1.
BC096476 mRNA. Translation: AAH96476.1.
BC120685 mRNA. Translation: AAI20686.1.
CCDSiCCDS28003.1. [Q3TY86-2]
CCDS79430.1. [Q3TY86-1]
RefSeqiNP_001277999.1. NM_001291070.1. [Q3TY86-1]
NP_780387.2. NM_175178.4. [Q3TY86-2]
XP_006522655.1. XM_006522592.2. [Q3TY86-1]
UniGeneiMm.40038.

3D structure databases

ProteinModelPortaliQ3TY86.
SMRiQ3TY86. Positions 72-170, 192-562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023448.

PTM databases

iPTMnetiQ3TY86.
PhosphoSiteiQ3TY86.

Proteomic databases

MaxQBiQ3TY86.
PaxDbiQ3TY86.
PRIDEiQ3TY86.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023448; ENSMUSP00000023448; ENSMUSG00000022763. [Q3TY86-2]
ENSMUST00000115685; ENSMUSP00000111349; ENSMUSG00000022763. [Q3TY86-1]
GeneIDi72168.
KEGGimmu:72168.
UCSCiuc007ykw.2. mouse. [Q3TY86-1]
uc007ykx.2. mouse. [Q3TY86-3]
uc007yky.2. mouse. [Q3TY86-2]

Organism-specific databases

CTDi150209.
MGIiMGI:1919418. Aifm3.

Phylogenomic databases

eggNOGiKOG1336. Eukaryota.
COG0446. LUCA.
COG2146. LUCA.
GeneTreeiENSGT00530000063416.
HOGENOMiHOG000276711.
HOVERGENiHBG052926.
InParanoidiQ3TY86.
OMAiLHSKTGD.
OrthoDBiEOG77DJ5F.
PhylomeDBiQ3TY86.
TreeFamiTF314028.

Miscellaneous databases

PROiQ3TY86.
SOURCEiSearch...

Gene expression databases

BgeeiQ3TY86.
GenevisibleiQ3TY86. MM.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.30.390.30. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
IPR028202. Reductase_C.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
PF14759. Reductase_C. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSiPR00469. PNDRDTASEII.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF51905. SSF51905. 1 hit.
SSF55424. SSF55424. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Hippocampus and Visual cortex.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Strain: FVB/N.
    Tissue: Brain and Kidney.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiAIFM3_MOUSE
AccessioniPrimary (citable) accession number: Q3TY86
Secondary accession number(s): Q0VBD6, Q4VAA4, Q8BWV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: October 11, 2005
Last modified: June 8, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.