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Protein

Vesicular glutamate transporter 1

Gene

Slc17a7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.3 Publications

GO - Molecular functioni

GO - Biological processi

  • brain development Source: Ensembl
  • chemical synaptic transmission Source: MGI
  • excitatory postsynaptic potential Source: MGI
  • L-glutamate import Source: MGI
  • long-term memory Source: MGI
  • neural retina development Source: Ensembl
  • neurotransmitter transport Source: UniProtKB-KW
  • sequestering of neurotransmitter Source: MGI
  • sodium ion transmembrane transport Source: GO_Central
  • synaptic transmission, glutamatergic Source: MGI
  • synaptic vesicle lumen acidification Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Neurotransmitter transport, Sensory transduction, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiR-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-428643. Organic anion transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicular glutamate transporter 1
Short name:
VGluT1
Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7
Gene namesi
Name:Slc17a7
Synonyms:Bnpi, Vglut1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1920211. Slc17a7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 63CytoplasmicSequence analysisAdd BLAST63
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Topological domaini85 – 116ExtracellularSequence analysisAdd BLAST32
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 140CytoplasmicSequence analysis3
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 169ExtracellularSequence analysis8
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Topological domaini191 – 208CytoplasmicSequence analysisAdd BLAST18
Transmembranei209 – 229HelicalSequence analysisAdd BLAST21
Topological domaini230 – 236ExtracellularSequence analysis7
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 302CytoplasmicSequence analysisAdd BLAST45
Transmembranei303 – 323HelicalSequence analysisAdd BLAST21
Topological domaini324 – 341ExtracellularSequence analysisAdd BLAST18
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 378CytoplasmicSequence analysisAdd BLAST16
Transmembranei379 – 399HelicalSequence analysisAdd BLAST21
Topological domaini400 – 401ExtracellularSequence analysis2
Transmembranei402 – 422HelicalSequence analysisAdd BLAST21
Topological domaini423 – 435CytoplasmicSequence analysisAdd BLAST13
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 469ExtracellularSequence analysisAdd BLAST13
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 560CytoplasmicSequence analysisAdd BLAST70

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cerebellar mossy fiber Source: MGI
  • excitatory synapse Source: MGI
  • integral component of membrane Source: MGI
  • postsynapse Source: GOC
  • presynaptic active zone Source: BHF-UCL
  • synapse Source: MGI
  • synaptic vesicle Source: MGI
  • synaptic vesicle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Disruption phenotypei

Mice begin to die 3 weeks after birth. They exhibit a progressive neurological phenotype including blindness, loss of coordination and enhanced startle response. Glutamatergic neurotransmission is drastically reduced due to a decrease in the reserve pool of synaptic vesicles and reduced quantal size. Visual signaling from photoreceptors to retinal output neurons is impaired while photoentrainment and pupillary light responses remain intact.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003316121 – 560Vesicular glutamate transporter 1Add BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei504PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3TXX4.
PaxDbiQ3TXX4.
PeptideAtlasiQ3TXX4.
PRIDEiQ3TXX4.

PTM databases

iPTMnetiQ3TXX4.
PhosphoSitePlusiQ3TXX4.
SwissPalmiQ3TXX4.

Expressioni

Tissue specificityi

Expressed in hippocampus (at protein level). Expressed in the molecular layer of the cerebellum and in retina.4 Publications

Developmental stagei

Expression in brain increases progressively from four days to adulthood.2 Publications

Inductioni

Oscillates diurnally in synaptic vesicles (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000070570.
GenevisibleiQ3TXX4. MM.

Interactioni

Subunit structurei

Interacts with SHANK3.1 Publication

Protein-protein interaction databases

IntActiQ3TXX4. 1 interactor.
STRINGi10090.ENSMUSP00000082489.

Structurei

3D structure databases

ProteinModelPortaliQ3TXX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi530 – 556Pro-richAdd BLAST27

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ3TXX4.
KOiK12302.
OMAiFSWAMAG.
OrthoDBiEOG091G06DG.
TreeFamiTF313535.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3TXX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP
60 70 80 90 100
VVDCTCFGLP RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG
110 120 130 140 150
HVVVQKAQFN WDPETVGLIH GSFFWGYIVT QIPGGFICQK FAANRVFGFA
160 170 180 190 200
IVATSTLNML IPSAARVHYG CVIFVRILQG LVEGVTYPAC HGIWSKWAPP
210 220 230 240 250
LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV YGSFGIFWYL
260 270 280 290 300
FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT
310 320 330 340 350
SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV
360 370 380 390 400
MTIIVPIGGQ IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS
410 420 430 440 450
KGVAISFLVL AVGFSGFAIS GFNVNHLDIA PRYASILMGI SNGVGTLSGM
460 470 480 490 500
VCPIIVGAMT KHKTREEWQY VFLIASLVHY GGVIFYGVFA SGEKQPWAEP
510 520 530 540 550
EEMSEEKCGF VGHDQLAGSD ESEMEDEAEP PGAPPAPPPS YGATHSTVQP
560
PRPPPPVRDY
Length:560
Mass (Da):61,637
Last modified:July 27, 2011 - v2
Checksum:iE866668F606B8305
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti500P → Q in BAE34790 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159064 mRNA. Translation: BAE34790.1.
AC149868 Genomic DNA. No translation available.
CCDSiCCDS52244.1.
RefSeqiNP_892038.2. NM_182993.2.
UniGeneiMm.255631.

Genome annotation databases

EnsembliENSMUST00000085374; ENSMUSP00000082489; ENSMUSG00000070570.
GeneIDi72961.
KEGGimmu:72961.
UCSCiuc009gtx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159064 mRNA. Translation: BAE34790.1.
AC149868 Genomic DNA. No translation available.
CCDSiCCDS52244.1.
RefSeqiNP_892038.2. NM_182993.2.
UniGeneiMm.255631.

3D structure databases

ProteinModelPortaliQ3TXX4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3TXX4. 1 interactor.
STRINGi10090.ENSMUSP00000082489.

PTM databases

iPTMnetiQ3TXX4.
PhosphoSitePlusiQ3TXX4.
SwissPalmiQ3TXX4.

Proteomic databases

MaxQBiQ3TXX4.
PaxDbiQ3TXX4.
PeptideAtlasiQ3TXX4.
PRIDEiQ3TXX4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085374; ENSMUSP00000082489; ENSMUSG00000070570.
GeneIDi72961.
KEGGimmu:72961.
UCSCiuc009gtx.2. mouse.

Organism-specific databases

CTDi57030.
MGIiMGI:1920211. Slc17a7.

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230812.
HOVERGENiHBG008834.
InParanoidiQ3TXX4.
KOiK12302.
OMAiFSWAMAG.
OrthoDBiEOG091G06DG.
TreeFamiTF313535.

Enzyme and pathway databases

ReactomeiR-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-428643. Organic anion transporters.

Miscellaneous databases

ChiTaRSiSlc17a7. mouse.
PROiQ3TXX4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070570.
GenevisibleiQ3TXX4. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVGLU1_MOUSE
AccessioniPrimary (citable) accession number: Q3TXX4
Secondary accession number(s): E9QMT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.