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Q3TXX4 (VGLU1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vesicular glutamate transporter 1

Short name=VGluT1
Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7
Gene names
Name:Slc17a7
Synonyms:Bnpi, Vglut1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Ref.4 Ref.5 Ref.7

Subunit structure

Interacts with SHANK3. Ref.8

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane. Membrane; Multi-pass membrane protein Potential. Cell junctionsynapsesynaptosome Ref.6 Ref.7 Ref.8.

Tissue specificity

Expressed in hippocampus (at protein level). Expressed in the molecular layer of the cerebellum and in retina. Ref.4 Ref.7 Ref.8

Developmental stage

Expression in brain increases progressively from four days to adulthood. Ref.3 Ref.5

Disruption phenotype

Mice begin to die 3 weeks after birth. They exhibit a progressive neurological phenotype including blindness, loss of coordination and enhanced startle response. Glutamatergic neurotransmission is drastically reduced due to a decrease in the reserve pool of synaptic vesicles and reduced quantal size. Visual signaling from photoreceptors to retinal output neurons is impaired while photoentrainment and pupillary light responses remain intact. Ref.4 Ref.5 Ref.7

Sequence similarities

Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Ontologies

Keywords
   Biological processIon transport
Neurotransmitter transport
Sensory transduction
Sodium transport
Symport
Transport
   Cellular componentCell junction
Cytoplasmic vesicle
Membrane
Synapse
Synaptosome
   DomainTransmembrane
Transmembrane helix
   LigandSodium
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-glutamate import

Inferred from direct assay PubMed 15860731. Source: MGI

long-term memory

Inferred from mutant phenotype PubMed 17241289. Source: MGI

neurotransmitter transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of excitatory postsynaptic membrane potential

Inferred from mutant phenotype Ref.5. Source: MGI

sequestering of neurotransmitter

Inferred from mutant phenotype Ref.4. Source: MGI

synaptic transmission

Inferred from mutant phenotype Ref.7. Source: MGI

synaptic transmission, glutamatergic

Inferred from mutant phenotype Ref.5. Source: MGI

   Cellular_componentcell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cerebellar mossy fiber

Inferred from direct assay PubMed 23791195. Source: MGI

excitatory synapse

Inferred from direct assay PubMed 16980967PubMed 17823315PubMed 21356198. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

presynaptic active zone

Inferred from direct assay PubMed 18986540. Source: BHF-UCL

synapse

Inferred from direct assay Ref.5PubMed 16814779. Source: MGI

synaptic vesicle

Inferred from direct assay PubMed 15579147. Source: MGI

synaptic vesicle membrane

Inferred from direct assay PubMed 15860731. Source: MGI

   Molecular_functioninorganic phosphate transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

sodium-dependent phosphate transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

sodium:inorganic phosphate symporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Vesicular glutamate transporter 1
PRO_0000331612

Regions

Topological domain1 – 6363Cytoplasmic Potential
Transmembrane64 – 8421Helical; Potential
Topological domain85 – 11632Extracellular Potential
Transmembrane117 – 13721Helical; Potential
Topological domain138 – 1403Cytoplasmic Potential
Transmembrane141 – 16121Helical; Potential
Topological domain162 – 1698Extracellular Potential
Transmembrane170 – 19021Helical; Potential
Topological domain191 – 20818Cytoplasmic Potential
Transmembrane209 – 22921Helical; Potential
Topological domain230 – 2367Extracellular Potential
Transmembrane237 – 25721Helical; Potential
Topological domain258 – 30245Cytoplasmic Potential
Transmembrane303 – 32321Helical; Potential
Topological domain324 – 34118Extracellular Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 37816Cytoplasmic Potential
Transmembrane379 – 39921Helical; Potential
Topological domain400 – 4012Extracellular Potential
Transmembrane402 – 42221Helical; Potential
Topological domain423 – 43513Cytoplasmic Potential
Transmembrane436 – 45621Helical; Potential
Topological domain457 – 46913Extracellular Potential
Transmembrane470 – 49021Helical; Potential
Topological domain491 – 56070Cytoplasmic Potential
Compositional bias530 – 55627Pro-rich

Experimental info

Sequence conflict5001P → Q in BAE34790. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q3TXX4 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: E866668F606B8305

FASTA56061,637
        10         20         30         40         50         60 
MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP VVDCTCFGLP 

        70         80         90        100        110        120 
RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG HVVVQKAQFN WDPETVGLIH 

       130        140        150        160        170        180 
GSFFWGYIVT QIPGGFICQK FAANRVFGFA IVATSTLNML IPSAARVHYG CVIFVRILQG 

       190        200        210        220        230        240 
LVEGVTYPAC HGIWSKWAPP LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV 

       250        260        270        280        290        300 
YGSFGIFWYL FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT 

       310        320        330        340        350        360 
SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV MTIIVPIGGQ 

       370        380        390        400        410        420 
IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS KGVAISFLVL AVGFSGFAIS 

       430        440        450        460        470        480 
GFNVNHLDIA PRYASILMGI SNGVGTLSGM VCPIIVGAMT KHKTREEWQY VFLIASLVHY 

       490        500        510        520        530        540 
GGVIFYGVFA SGEKQPWAEP EEMSEEKCGF VGHDQLAGSD ESEMEDEAEP PGAPPAPPPS 

       550        560 
YGATHSTVQP PRPPPPVRDY 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Visual cortex.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons."
Schaefer M.K.-H., Varoqui H., Defamie N., Weihe E., Erickson J.D.
J. Biol. Chem. 277:50734-50748(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[4]"An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size."
Wojcik S.M., Rhee J.S., Herzog E., Sigler A., Jahn R., Takamori S., Brose N., Rosenmund C.
Proc. Natl. Acad. Sci. U.S.A. 101:7158-7163(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[5]"Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites."
Fremeau R.T. Jr., Kam K., Qureshi T., Johnson J., Copenhagen D.R., Storm-Mathisen J., Chaudhry F.A., Nicoll R.A., Edwards R.H.
Science 304:1815-1819(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[6]"Synaptic and vesicular co-localization of the glutamate transporters VGLUT1 and VGLUT2 in the mouse hippocampus."
Herzog E., Takamori S., Jahn R., Brose N., Wojcik S.M.
J. Neurochem. 99:1011-1018(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Vesicular glutamate transporter 1 is required for photoreceptor synaptic signaling but not for intrinsic visual functions."
Johnson J., Fremeau R.T. Jr., Duncan J.L., Renteria R.C., Yang H., Hua Z., Liu X., LaVail M.M., Edwards R.H., Copenhagen D.R.
J. Neurosci. 27:7245-7255(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties."
Han K., Holder J.L. Jr., Schaaf C.P., Lu H., Chen H., Kang H., Tang J., Wu Z., Hao S., Cheung S.W., Yu P., Sun H., Breman A.M., Patel A., Lu H.C., Zoghbi H.Y.
Nature 503:72-77(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SHANK3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK159064 mRNA. Translation: BAE34790.1.
AC149868 Genomic DNA. No translation available.
CCDSCCDS52244.1.
RefSeqNP_892038.2. NM_182993.2.
UniGeneMm.255631.

3D structure databases

ProteinModelPortalQ3TXX4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ3TXX4. 1 interaction.

PTM databases

PhosphoSiteQ3TXX4.

Proteomic databases

MaxQBQ3TXX4.
PaxDbQ3TXX4.
PRIDEQ3TXX4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000085374; ENSMUSP00000082489; ENSMUSG00000070570.
GeneID72961.
KEGGmmu:72961.
UCSCuc009gtx.2. mouse.

Organism-specific databases

CTD57030.
MGIMGI:1920211. Slc17a7.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00630000089608.
HOGENOMHOG000230812.
HOVERGENHBG008834.
InParanoidQ3TXX4.
KOK12302.
OMAMHGHVVI.
OrthoDBEOG789C9Z.
TreeFamTF313535.

Gene expression databases

BgeeQ3TXX4.
GenevestigatorQ3TXX4.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC17A7. mouse.
NextBio337233.
PROQ3TXX4.
SOURCESearch...

Entry information

Entry nameVGLU1_MOUSE
AccessionPrimary (citable) accession number: Q3TXX4
Secondary accession number(s): E9QMT8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot