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Q3TXX4

- VGLU1_MOUSE

UniProt

Q3TXX4 - VGLU1_MOUSE

Protein

Vesicular glutamate transporter 1

Gene

Slc17a7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.3 Publications

    GO - Molecular functioni

    1. inorganic phosphate transmembrane transporter activity Source: Ensembl
    2. sodium:inorganic phosphate symporter activity Source: Ensembl
    3. sodium-dependent phosphate transmembrane transporter activity Source: Ensembl

    GO - Biological processi

    1. L-glutamate import Source: MGI
    2. long-term memory Source: MGI
    3. neurotransmitter transport Source: UniProtKB-KW
    4. regulation of excitatory postsynaptic membrane potential Source: MGI
    5. sequestering of neurotransmitter Source: MGI
    6. synaptic transmission Source: MGI
    7. synaptic transmission, glutamatergic Source: MGI

    Keywords - Biological processi

    Ion transport, Neurotransmitter transport, Sensory transduction, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicular glutamate transporter 1
    Short name:
    VGluT1
    Alternative name(s):
    Brain-specific Na(+)-dependent inorganic phosphate cotransporter
    Solute carrier family 17 member 7
    Gene namesi
    Name:Slc17a7
    Synonyms:Bnpi, Vglut1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1920211. Slc17a7.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cerebellar mossy fiber Source: MGI
    3. excitatory synapse Source: MGI
    4. integral component of membrane Source: UniProtKB-KW
    5. presynaptic active zone Source: BHF-UCL
    6. synapse Source: MGI
    7. synaptic vesicle Source: MGI
    8. synaptic vesicle membrane Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

    Pathology & Biotechi

    Disruption phenotypei

    Mice begin to die 3 weeks after birth. They exhibit a progressive neurological phenotype including blindness, loss of coordination and enhanced startle response. Glutamatergic neurotransmission is drastically reduced due to a decrease in the reserve pool of synaptic vesicles and reduced quantal size. Visual signaling from photoreceptors to retinal output neurons is impaired while photoentrainment and pupillary light responses remain intact.3 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560Vesicular glutamate transporter 1PRO_0000331612Add
    BLAST

    Proteomic databases

    MaxQBiQ3TXX4.
    PaxDbiQ3TXX4.
    PRIDEiQ3TXX4.

    PTM databases

    PhosphoSiteiQ3TXX4.

    Expressioni

    Tissue specificityi

    Expressed in hippocampus (at protein level). Expressed in the molecular layer of the cerebellum and in retina.4 Publications

    Developmental stagei

    Expression in brain increases progressively from four days to adulthood.2 Publications

    Inductioni

    Oscillates diurnally in synaptic vesicles (at protein level).1 Publication

    Gene expression databases

    BgeeiQ3TXX4.
    GenevestigatoriQ3TXX4.

    Interactioni

    Subunit structurei

    Interacts with SHANK3.1 Publication

    Protein-protein interaction databases

    IntActiQ3TXX4. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3TXX4.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6363CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini85 – 11632ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini138 – 1403CytoplasmicSequence Analysis
    Topological domaini162 – 1698ExtracellularSequence Analysis
    Topological domaini191 – 20818CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini230 – 2367ExtracellularSequence Analysis
    Topological domaini258 – 30245CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini324 – 34118ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini363 – 37816CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini400 – 4012ExtracellularSequence Analysis
    Topological domaini423 – 43513CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini457 – 46913ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini491 – 56070CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei64 – 8421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei117 – 13721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei141 – 16121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei209 – 22921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei303 – 32321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei342 – 36221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei379 – 39921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei402 – 42221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei436 – 45621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei470 – 49021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi530 – 55627Pro-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00630000089608.
    HOGENOMiHOG000230812.
    HOVERGENiHBG008834.
    InParanoidiQ3TXX4.
    KOiK12302.
    OMAiMHGHVVI.
    OrthoDBiEOG789C9Z.
    TreeFamiTF313535.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3TXX4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP    50
    VVDCTCFGLP RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG 100
    HVVVQKAQFN WDPETVGLIH GSFFWGYIVT QIPGGFICQK FAANRVFGFA 150
    IVATSTLNML IPSAARVHYG CVIFVRILQG LVEGVTYPAC HGIWSKWAPP 200
    LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV YGSFGIFWYL 250
    FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT 300
    SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV 350
    MTIIVPIGGQ IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS 400
    KGVAISFLVL AVGFSGFAIS GFNVNHLDIA PRYASILMGI SNGVGTLSGM 450
    VCPIIVGAMT KHKTREEWQY VFLIASLVHY GGVIFYGVFA SGEKQPWAEP 500
    EEMSEEKCGF VGHDQLAGSD ESEMEDEAEP PGAPPAPPPS YGATHSTVQP 550
    PRPPPPVRDY 560
    Length:560
    Mass (Da):61,637
    Last modified:July 27, 2011 - v2
    Checksum:iE866668F606B8305
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti500 – 5001P → Q in BAE34790. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK159064 mRNA. Translation: BAE34790.1.
    AC149868 Genomic DNA. No translation available.
    CCDSiCCDS52244.1.
    RefSeqiNP_892038.2. NM_182993.2.
    UniGeneiMm.255631.

    Genome annotation databases

    EnsembliENSMUST00000085374; ENSMUSP00000082489; ENSMUSG00000070570.
    GeneIDi72961.
    KEGGimmu:72961.
    UCSCiuc009gtx.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK159064 mRNA. Translation: BAE34790.1 .
    AC149868 Genomic DNA. No translation available.
    CCDSi CCDS52244.1.
    RefSeqi NP_892038.2. NM_182993.2.
    UniGenei Mm.255631.

    3D structure databases

    ProteinModelPortali Q3TXX4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q3TXX4. 1 interaction.

    PTM databases

    PhosphoSitei Q3TXX4.

    Proteomic databases

    MaxQBi Q3TXX4.
    PaxDbi Q3TXX4.
    PRIDEi Q3TXX4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000085374 ; ENSMUSP00000082489 ; ENSMUSG00000070570 .
    GeneIDi 72961.
    KEGGi mmu:72961.
    UCSCi uc009gtx.2. mouse.

    Organism-specific databases

    CTDi 57030.
    MGIi MGI:1920211. Slc17a7.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00630000089608.
    HOGENOMi HOG000230812.
    HOVERGENi HBG008834.
    InParanoidi Q3TXX4.
    KOi K12302.
    OMAi MHGHVVI.
    OrthoDBi EOG789C9Z.
    TreeFami TF313535.

    Miscellaneous databases

    ChiTaRSi SLC17A7. mouse.
    NextBioi 337233.
    PROi Q3TXX4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3TXX4.
    Genevestigatori Q3TXX4.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Visual cortex.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons."
      Schaefer M.K.-H., Varoqui H., Defamie N., Weihe E., Erickson J.D.
      J. Biol. Chem. 277:50734-50748(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    4. "An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size."
      Wojcik S.M., Rhee J.S., Herzog E., Sigler A., Jahn R., Takamori S., Brose N., Rosenmund C.
      Proc. Natl. Acad. Sci. U.S.A. 101:7158-7163(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
    5. "Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites."
      Fremeau R.T. Jr., Kam K., Qureshi T., Johnson J., Copenhagen D.R., Storm-Mathisen J., Chaudhry F.A., Nicoll R.A., Edwards R.H.
      Science 304:1815-1819(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    6. "Synaptic and vesicular co-localization of the glutamate transporters VGLUT1 and VGLUT2 in the mouse hippocampus."
      Herzog E., Takamori S., Jahn R., Brose N., Wojcik S.M.
      J. Neurochem. 99:1011-1018(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Differential sorting of the vesicular glutamate transporter 1 into a defined vesicular pool is regulated by light signaling involving the clock gene Period2."
      Yelamanchili S.V., Pendyala G., Brunk I., Darna M., Albrecht U., Ahnert-Hilger G.
      J. Biol. Chem. 281:15671-15679(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. "Vesicular glutamate transporter 1 is required for photoreceptor synaptic signaling but not for intrinsic visual functions."
      Johnson J., Fremeau R.T. Jr., Duncan J.L., Renteria R.C., Yang H., Hua Z., Liu X., LaVail M.M., Edwards R.H., Copenhagen D.R.
      J. Neurosci. 27:7245-7255(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    9. "SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties."
      Han K., Holder J.L. Jr., Schaaf C.P., Lu H., Chen H., Kang H., Tang J., Wu Z., Hao S., Cheung S.W., Yu P., Sun H., Breman A.M., Patel A., Lu H.C., Zoghbi H.Y.
      Nature 503:72-77(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SHANK3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiVGLU1_MOUSE
    AccessioniPrimary (citable) accession number: Q3TXX4
    Secondary accession number(s): E9QMT8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3