Q3TXX4 (VGLU1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vesicular glutamate transporter 1
Short name=VGluT1

Alternative name(s):
Brain-specific Na(+)-dependent inorganic phosphate cotransporter
Solute carrier family 17 member 7

Gene names

Name:	Slc17a7
Synonyms:	Bnpi, Vglut1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Ref.4 Ref.5 Ref.7
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Membrane; Multi-pass membrane protein Potential. Cell junction › synapse › synaptosome Ref.6 Ref.7.
Tissue specificity	Expressed in the molecular layer of the cerebellum and in retina. Ref.4 Ref.7
Developmental stage	Expression in brain increases progressively from four days to adulthood. Ref.3 Ref.5
Disruption phenotype	Mice begin to die 3 weeks after birth. They exhibit a progressive neurological phenotype including blindness, loss of coordination and enhanced startle response. Glutamatergic neurotransmission is drastically reduced due to a decrease in the reserve pool of synaptic vesicles and reduced quantal size. Visual signaling from photoreceptors to retinal output neurons is impaired while photoentrainment and pupillary light responses remain intact. Ref.4 Ref.5 Ref.7
Sequence similarities	Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Ontologies

Keywords
Biological process	Ion transport Neurotransmitter transport Sensory transduction Sodium transport Symport Transport
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse Synaptosome
Domain	Transmembrane Transmembrane helix
Ligand	Sodium
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-glutamate import Inferred from direct assay PubMed 15860731. Source: MGI long-term memory Inferred from mutant phenotype PubMed 17241289. Source: MGI neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of excitatory postsynaptic membrane potential Inferred from mutant phenotype Ref.5. Source: MGI sequestering of neurotransmitter Inferred from mutant phenotype Ref.4. Source: MGI synaptic transmission, glutamatergic Inferred from mutant phenotype Ref.5. Source: MGI
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW excitatory synapse Inferred from direct assay PubMed 17823315PubMed 21356198. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from electronic annotation. Source: UniProtKB-SubCell presynaptic active zone Inferred from direct assay PubMed 18986540. Source: BHF-UCL synaptic vesicle membrane Inferred from direct assay PubMed 15860731. Source: MGI
Molecular_function	inorganic phosphate transmembrane transporter activity Inferred from electronic annotation. Source: Compara sodium-dependent phosphate transmembrane transporter activity Inferred from electronic annotation. Source: Compara sodium:inorganic phosphate symporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Vesicular glutamate transporter 1		PRO_0000331612
Regions
	Topological domain	1 – 63	63	Cytoplasmic Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Topological domain	85 – 116	32	Extracellular Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Topological domain	138 – 140	3	Cytoplasmic Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Topological domain	162 – 169	8	Extracellular Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Topological domain	191 – 208	18	Cytoplasmic Potential
	Transmembrane	209 – 229	21	Helical; Potential
	Topological domain	230 – 236	7	Extracellular Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Topological domain	258 – 302	45	Cytoplasmic Potential
	Transmembrane	303 – 323	21	Helical; Potential
	Topological domain	324 – 341	18	Extracellular Potential
	Transmembrane	342 – 362	21	Helical; Potential
	Topological domain	363 – 378	16	Cytoplasmic Potential
	Transmembrane	379 – 399	21	Helical; Potential
	Topological domain	400 – 401	2	Extracellular Potential
	Transmembrane	402 – 422	21	Helical; Potential
	Topological domain	423 – 435	13	Cytoplasmic Potential
	Transmembrane	436 – 456	21	Helical; Potential
	Topological domain	457 – 469	13	Extracellular Potential
	Transmembrane	470 – 490	21	Helical; Potential
	Topological domain	491 – 560	70	Cytoplasmic Potential
	Compositional bias	530 – 556	27	Pro-rich
Experimental info
	Sequence conflict	500	1	P → Q in BAE34790. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q3TXX4 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: E866668F606B8305	FASTA	560	61,637
	10 20 30 40 50 60 MEFRQEEFRK LAGRALGRLH RLLEKRQEGA ETLELSADGR PVTTHTRDPP VVDCTCFGLP 70 80 90 100 110 120 RRYIIAIMSG LGFCISFGIR CNLGVAIVSM VNNSTTHRGG HVVVQKAQFN WDPETVGLIH 130 140 150 160 170 180 GSFFWGYIVT QIPGGFICQK FAANRVFGFA IVATSTLNML IPSAARVHYG CVIFVRILQG 190 200 210 220 230 240 LVEGVTYPAC HGIWSKWAPP LERSRLATTA FCGSYAGAVV AMPLAGVLVQ YSGWSSVFYV 250 260 270 280 290 300 YGSFGIFWYL FWLLVSYESP ALHPSISEEE RKYIEDAIGE SAKLMNPVTK FNTPWRRFFT 310 320 330 340 350 360 SMPVYAIIVA NFCRSWTFYL LLISQPAYFE EVFGFEISKV GLVSALPHLV MTIIVPIGGQ 370 380 390 400 410 420 IADFLRSRHI MSTTNVRKLM NCGGFGMEAT LLLVVGYSHS KGVAISFLVL AVGFSGFAIS 430 440 450 460 470 480 GFNVNHLDIA PRYASILMGI SNGVGTLSGM VCPIIVGAMT KHKTREEWQY VFLIASLVHY 490 500 510 520 530 540 GGVIFYGVFA SGEKQPWAEP EEMSEEKCGF VGHDQLAGSD ESEMEDEAEP PGAPPAPPPS 550 560 YGATHSTVQP PRPPPPVRDY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Visual cortex.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons." Schaefer M.K.-H., Varoqui H., Defamie N., Weihe E., Erickson J.D. J. Biol. Chem. 277:50734-50748(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[4]	"An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size." Wojcik S.M., Rhee J.S., Herzog E., Sigler A., Jahn R., Takamori S., Brose N., Rosenmund C. Proc. Natl. Acad. Sci. U.S.A. 101:7158-7163(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[5]	"Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites." Fremeau R.T. Jr., Kam K., Qureshi T., Johnson J., Copenhagen D.R., Storm-Mathisen J., Chaudhry F.A., Nicoll R.A., Edwards R.H. Science 304:1815-1819(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
[6]	"Synaptic and vesicular co-localization of the glutamate transporters VGLUT1 and VGLUT2 in the mouse hippocampus." Herzog E., Takamori S., Jahn R., Brose N., Wojcik S.M. J. Neurochem. 99:1011-1018(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Vesicular glutamate transporter 1 is required for photoreceptor synaptic signaling but not for intrinsic visual functions." Johnson J., Fremeau R.T. Jr., Duncan J.L., Renteria R.C., Yang H., Hua Z., Liu X., LaVail M.M., Edwards R.H., Copenhagen D.R. J. Neurosci. 27:7245-7255(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK159064 mRNA. Translation: BAE34790.1. AC149868 Genomic DNA. No translation available.
IPI	IPI00109153.
RefSeq	NP_892038.2. NM_182993.2.
UniGene	Mm.255631.
3D structure databases
ProteinModelPortal	Q3TXX4.
ModBase	Search...
PTM databases
PhosphoSite	Q3TXX4.
Proteomic databases
PaxDb	Q3TXX4.
PRIDE	Q3TXX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000085374; ENSMUSP00000082489; ENSMUSG00000070570.
GeneID	72961.
KEGG	mmu:72961.
UCSC	uc009gtx.2. mouse.
Organism-specific databases
CTD	57030.
MGI	MGI:1920211. Slc17a7.
Phylogenomic databases
eggNOG	COG0477.
GeneTree	ENSGT00630000089608.
HOGENOM	HOG000230812.
HOVERGEN	HBG008834.
InParanoid	Q3TXX4.
KO	K12302.
OMA	MHGHVVI.
OrthoDB	EOG4XWFXC.
Gene expression databases
ArrayExpress	Q3TXX4.
Bgee	Q3TXX4.
Genevestigator	Q3TXX4.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC17A7. mouse.
NextBio	337233.
SOURCE	Search...

Entry information

Entry name

VGLU1_MOUSE

Accession

Primary (citable) accession number: Q3TXX4
Secondary accession number(s): E9QMT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program