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Protein

Protrudin

Gene

Zfyve27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport (By similarity). Promotes axonal elongation and contributes to the establishment of neuronal cell polarity (PubMed:24251978). Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner. Contributes to both the formation and stabilization of the tubular ER network. Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections (By similarity). Acts as an adapter protein that facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner (PubMed:21976701).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri348 – 414FYVE-typePROSITE-ProRule annotationAdd BLAST67

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protrudin
Alternative name(s):
Zinc finger FYVE domain-containing protein 27
Gene namesi
Name:Zfyve27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1919602 Zfyve27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 66Cytoplasmic1 PublicationAdd BLAST66
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88Lumenal1 Publication1
Transmembranei89 – 109HelicalSequence analysisAdd BLAST21
Topological domaini110 – 192Cytoplasmic1 PublicationAdd BLAST83
Intramembranei193 – 213HelicalSequence analysisAdd BLAST21
Topological domaini214 – 415Cytoplasmic1 PublicationAdd BLAST202

Keywords - Cellular componenti

Cell membrane, Cell projection, Endoplasmic reticulum, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002456021 – 415ProtrudinAdd BLAST415

Post-translational modificationi

Phosphorylated. Phosphorylation is induced by NGF through the MAPK/ERK pathway and modulates interaction with RAB11A (Probable).1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3TXX3
PaxDbiQ3TXX3
PeptideAtlasiQ3TXX3
PRIDEiQ3TXX3

PTM databases

iPTMnetiQ3TXX3
PhosphoSitePlusiQ3TXX3

Expressioni

Tissue specificityi

Astrocytes express both isoform 1 and isoform 2 and oligodendrocytes express only isoform 2 (at protein level). Isoform 1 is expressed specifically in the central nervous system and selectively in neuronal cells. Isoform 2 is expressed in cerebrum, cerebellum, spinal cord, heart, thymus, spleen, intestine and lung.2 Publications

Gene expression databases

BgeeiENSMUSG00000018820
CleanExiMM_ZFYVE27
ExpressionAtlasiQ3TXX3 baseline and differential
GenevisibleiQ3TXX3 MM

Interactioni

Subunit structurei

Can form homooligomers (monomers, dimers and tetramers) (By similarity). Interacts with RAB11A (GDP-bound form); regulates RAB11A (PubMed:21976701). Interacts with FKBP8; may negatively regulate ZFYVE27 phosphorylation (By similarity). Isoform 1 interacts to a greater extent than isoform 2 with VAPB (via MSP domain). Isoform 1 interacts to a greater extent than isoform 2 with VAPA (via MSP domain) (PubMed:24251978). Interaction with VAPA may regulate ZFYVE27 retention in the endoplasmic reticulum and its function in cell projections formation. Interacts with ATL2, ATL3, SPAST and RTN3 (By similarity). Interacts with REEP1, REEP5 and ATL1 (PubMed:24668814). Interacts with RAB11B (GDP-bound form), SURF4, KIF5B and KIF5C (PubMed:21976701). Isoform 1 and 2 interact with KIFA (PubMed:21976701, PubMed:24251978).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ3TXX3
IntActiQ3TXX3 1 interactor.
STRINGi10090.ENSMUSP00000130684

Structurei

3D structure databases

ProteinModelPortaliQ3TXX3
SMRiQ3TXX3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 210Sufficient for localization to endoplasmic reticulum tubular network and for interactions with REEP1, REEP5, ATL1, ATL2, ATL3 and SPASTBy similarityAdd BLAST210
Regioni1 – 92Sufficient for homooligomerizationBy similarityAdd BLAST92
Regioni51 – 64Necessary for interaction with RAB11A and function in neurite outgrowthBy similarityAdd BLAST14
Regioni275 – 365Necessary for interaction with KIF5ABy similarityAdd BLAST91
Regioni290 – 296Necessary for interaction with VAPABy similarity7

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri348 – 414FYVE-typePROSITE-ProRule annotationAdd BLAST67

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJBT Eukaryota
ENOG4111KCR LUCA
GeneTreeiENSGT00390000013298
HOGENOMiHOG000155785
HOVERGENiHBG054907
InParanoidiQ3TXX3
KOiK19368
OMAiRCCSFKV
OrthoDBiEOG091G0QCA
PhylomeDBiQ3TXX3
TreeFamiTF331044

Family and domain databases

Gene3Di3.30.40.101 hit
InterProiView protein in InterPro
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SUPFAMiSSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50178 ZF_FYVE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TXX3-1) [UniParc]FASTAAdd to basket
Also known as: Protrudin-L1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTSDRDLSG PEASPSGMPE VLSECPPAPT KSAAFDLFNL VLSYKRLEIY
60 70 80 90 100
LEPLKDAGDG VRYLLRWQMP LCSLLTCLGL NILFLTLNEG AWYSMGALMI
110 120 130 140 150
SVPALLGYLQ EVCRGQLPES ELMRRKYHSI RQEDLQRVRL SRVHLSRPEA
160 170 180 190 200
VAEVKSFLIQ LEAFLARLCY TCESAYRVLH WENPVVSSQF YGALLGMVCM
210 220 230 240 250
LYLLPLCWVL ALLNSTLFLG NGDFFRVVCE YRACLQRRMN PRQEECACES
260 270 280 290 300
SALQGAGGRG LLDSSPAPTP TEDLTPGSVE EAEEAEPDEE FKDAIEETHL
310 320 330 340 350
VVLEDEEGTP CPAEDELTLQ DNGFLSKNEV LRSKVSRLTE RLRKRYPTNN
360 370 380 390 400
FGNCAGCAAT FSVLKKRRSC SNCGNSFCSR CCSFKVPRSS MGATAPEAQR
410
ETVCVCASCN QTLSK
Length:415
Mass (Da):46,202
Last modified:July 11, 2006 - v2
Checksum:iEC11945BFEDC76D5
GO
Isoform 2 (identifier: Q3TXX3-2) [UniParc]FASTAAdd to basket
Also known as: Protrudin-S1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     297-303: Missing.

Show »
Length:408
Mass (Da):45,410
Checksum:iD349DAF62499DAE1
GO

Sequence cautioni

The sequence AAH42595 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41V → A in BAE34791 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019757297 – 303Missing in isoform 2. 2 Publications7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159065 mRNA Translation: BAE34791.1
BC042595 mRNA Translation: AAH42595.1 Different initiation.
CCDSiCCDS29825.2 [Q3TXX3-1]
CCDS50440.1 [Q3TXX3-2]
RefSeqiNP_001158003.1, NM_001164531.1 [Q3TXX3-2]
NP_796293.2, NM_177319.3 [Q3TXX3-1]
UniGeneiMm.287359
Mm.490064

Genome annotation databases

EnsembliENSMUST00000099443; ENSMUSP00000097042; ENSMUSG00000018820 [Q3TXX3-2]
ENSMUST00000169536; ENSMUSP00000130684; ENSMUSG00000018820 [Q3TXX3-1]
GeneIDi319740
KEGGimmu:319740
UCSCiuc008hnj.2 mouse [Q3TXX3-2]
uc012blu.1 mouse [Q3TXX3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZFY27_MOUSE
AccessioniPrimary (citable) accession number: Q3TXX3
Secondary accession number(s): Q8CFP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: April 25, 2018
This is version 92 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome