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Protein

Protrudin

Gene

Zfyve27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Involved in nerve growth factor-induced neurite formation. May have a more general role in cell projections formation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri348 – 41467FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protrudin
Alternative name(s):
Zinc finger FYVE domain-containing protein 27
Gene namesi
Name:Zfyve27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1919602. Zfyve27.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6666CytoplasmicSequence analysisAdd
BLAST
Transmembranei67 – 8721HelicalSequence analysisAdd
BLAST
Topological domaini88 – 881ExtracellularSequence analysis
Transmembranei89 – 10921HelicalSequence analysisAdd
BLAST
Topological domaini110 – 19283CytoplasmicSequence analysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST
Topological domaini214 – 415202ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • dendrite Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • endoplasmic reticulum tubular network Source: MGI
  • growth cone membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • recycling endosome membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Endoplasmic reticulum, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415ProtrudinPRO_0000245602Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi214 – 2141N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylated. Phosphorylation is induced by NGF through the MAPK/ERK pathway and modulates interaction with RAB11A (Probable).1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ3TXX3.
MaxQBiQ3TXX3.
PaxDbiQ3TXX3.
PeptideAtlasiQ3TXX3.
PRIDEiQ3TXX3.

PTM databases

iPTMnetiQ3TXX3.
PhosphoSiteiQ3TXX3.

Expressioni

Tissue specificityi

Most abundant in cerebrum and cerebellum. Also detected in heart, thymus, spleen, intestine and lung.1 Publication

Gene expression databases

BgeeiQ3TXX3.
CleanExiMM_ZFYVE27.
ExpressionAtlasiQ3TXX3. baseline and differential.
GenevisibleiQ3TXX3. MM.

Interactioni

Subunit structurei

Interacts with SPAST. Interacts with RAB11A (GDP-bound form); regulates RAB11A. Interacts with FKBP8; may negatively regulate ZFYVE27 phosphorylation. Interacts with VAPA (via MSP domain); may regulate ZFYVE27 retention in the endoplasmic reticulum and its function in cell projections formation. Interacts with VAPB (via MSP domain) (By similarity).By similarity

Protein-protein interaction databases

IntActiQ3TXX3. 1 interaction.
STRINGi10090.ENSMUSP00000130684.

Structurei

3D structure databases

ProteinModelPortaliQ3TXX3.
SMRiQ3TXX3. Positions 345-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 6414Necessary for interaction with RAB11A and function in neurite outgrowthBy similarityAdd
BLAST
Regioni290 – 2967Necessary for interaction with VAPABy similarity

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri348 – 41467FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJBT. Eukaryota.
ENOG4111KCR. LUCA.
GeneTreeiENSGT00390000013298.
HOGENOMiHOG000155785.
HOVERGENiHBG054907.
InParanoidiQ3TXX3.
KOiK19368.
OMAiTVCMLYL.
OrthoDBiEOG7VTDNG.
PhylomeDBiQ3TXX3.
TreeFamiTF331044.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3TXX3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQTSDRDLSG PEASPSGMPE VLSECPPAPT KSAAFDLFNL VLSYKRLEIY
60 70 80 90 100
LEPLKDAGDG VRYLLRWQMP LCSLLTCLGL NILFLTLNEG AWYSMGALMI
110 120 130 140 150
SVPALLGYLQ EVCRGQLPES ELMRRKYHSI RQEDLQRVRL SRVHLSRPEA
160 170 180 190 200
VAEVKSFLIQ LEAFLARLCY TCESAYRVLH WENPVVSSQF YGALLGMVCM
210 220 230 240 250
LYLLPLCWVL ALLNSTLFLG NGDFFRVVCE YRACLQRRMN PRQEECACES
260 270 280 290 300
SALQGAGGRG LLDSSPAPTP TEDLTPGSVE EAEEAEPDEE FKDAIEETHL
310 320 330 340 350
VVLEDEEGTP CPAEDELTLQ DNGFLSKNEV LRSKVSRLTE RLRKRYPTNN
360 370 380 390 400
FGNCAGCAAT FSVLKKRRSC SNCGNSFCSR CCSFKVPRSS MGATAPEAQR
410
ETVCVCASCN QTLSK
Length:415
Mass (Da):46,202
Last modified:July 11, 2006 - v2
Checksum:iEC11945BFEDC76D5
GO
Isoform 2 (identifier: Q3TXX3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     298-304: Missing.

Show »
Length:408
Mass (Da):45,410
Checksum:iD349DAF62499DAE1
GO

Sequence cautioni

The sequence AAH42595.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti41 – 411V → A in BAE34791 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei298 – 3047Missing in isoform 2. 1 PublicationVSP_019757

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159065 mRNA. Translation: BAE34791.1.
BC042595 mRNA. Translation: AAH42595.1. Different initiation.
CCDSiCCDS29825.2. [Q3TXX3-1]
CCDS50440.1. [Q3TXX3-2]
RefSeqiNP_001158003.1. NM_001164531.1. [Q3TXX3-2]
NP_796293.2. NM_177319.3. [Q3TXX3-1]
UniGeneiMm.287359.
Mm.490064.

Genome annotation databases

EnsembliENSMUST00000099443; ENSMUSP00000097042; ENSMUSG00000018820. [Q3TXX3-2]
ENSMUST00000169536; ENSMUSP00000130684; ENSMUSG00000018820. [Q3TXX3-1]
GeneIDi319740.
KEGGimmu:319740.
UCSCiuc008hnj.2. mouse. [Q3TXX3-2]
uc012blu.1. mouse. [Q3TXX3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159065 mRNA. Translation: BAE34791.1.
BC042595 mRNA. Translation: AAH42595.1. Different initiation.
CCDSiCCDS29825.2. [Q3TXX3-1]
CCDS50440.1. [Q3TXX3-2]
RefSeqiNP_001158003.1. NM_001164531.1. [Q3TXX3-2]
NP_796293.2. NM_177319.3. [Q3TXX3-1]
UniGeneiMm.287359.
Mm.490064.

3D structure databases

ProteinModelPortaliQ3TXX3.
SMRiQ3TXX3. Positions 345-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3TXX3. 1 interaction.
STRINGi10090.ENSMUSP00000130684.

PTM databases

iPTMnetiQ3TXX3.
PhosphoSiteiQ3TXX3.

Proteomic databases

EPDiQ3TXX3.
MaxQBiQ3TXX3.
PaxDbiQ3TXX3.
PeptideAtlasiQ3TXX3.
PRIDEiQ3TXX3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099443; ENSMUSP00000097042; ENSMUSG00000018820. [Q3TXX3-2]
ENSMUST00000169536; ENSMUSP00000130684; ENSMUSG00000018820. [Q3TXX3-1]
GeneIDi319740.
KEGGimmu:319740.
UCSCiuc008hnj.2. mouse. [Q3TXX3-2]
uc012blu.1. mouse. [Q3TXX3-1]

Organism-specific databases

CTDi118813.
MGIiMGI:1919602. Zfyve27.

Phylogenomic databases

eggNOGiENOG410IJBT. Eukaryota.
ENOG4111KCR. LUCA.
GeneTreeiENSGT00390000013298.
HOGENOMiHOG000155785.
HOVERGENiHBG054907.
InParanoidiQ3TXX3.
KOiK19368.
OMAiTVCMLYL.
OrthoDBiEOG7VTDNG.
PhylomeDBiQ3TXX3.
TreeFamiTF331044.

Miscellaneous databases

ChiTaRSiZfyve27. mouse.
PROiQ3TXX3.
SOURCEiSearch...

Gene expression databases

BgeeiQ3TXX3.
CleanExiMM_ZFYVE27.
ExpressionAtlasiQ3TXX3. baseline and differential.
GenevisibleiQ3TXX3. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Visual cortex.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Eye.
  3. "Protrudin induces neurite formation by directional membrane trafficking."
    Shirane M., Nakayama K.I.
    Science 314:818-821(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "Regulation of apoptosis and neurite extension by FKBP38 is required for neural tube formation in the mouse."
    Shirane M., Ogawa M., Motoyama J., Nakayama K.I.
    Genes Cells 13:635-651(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.

Entry informationi

Entry nameiZFY27_MOUSE
AccessioniPrimary (citable) accession number: Q3TXX3
Secondary accession number(s): Q8CFP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: July 6, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.